Abstract: The genetic diversity and relationships of 98 germplasms of five species and one variety of Zoysia were analyzed using SRAP markers. A total of 362 bands were detected with 54 pairs of SRAP primers and 337 of the bands were polymorphic (PPB=93.09%). The Nei’s gene diversity index (H) was 0.240 9 and the Shannon diversity index (I) was 0.374 6, indicating a high level of genetic diversity of zoysiagrass. The genetic similarity (GS) among 96 Zoysia germplasms ranged from 0.593 9 to 0.983 4 with an average of 0.758. Eight showed that a large genetic polymorphism existed among the germplasms of Zoysia grass. The GS between Z. japonica and Z. sinica, and between Z. matrella and Z. tenuifolia were relatively high compared with the GS of other species. The variance ranges of GS within species of Z. japonica, Z. sinica and Z. matrella were 0.599 4－0.983 4, 0.624 3－0.980 7 and 0.663 0－0.947 5, respectively. Based on the presence of bands, 96 zoysia germplasm were classified into seven major groups by UPGMA cluster analysis, in which group I and group II included three species of Z. Japonica, Z. sinica and a few accessions of Z. matrella. Group III included four accessions introduced from America. Group IV mainly comsisted of Z. matrella and Z. tenuifolia. Z. machrostachya (Z010), Z .sinica var nipponica (Z122) and Z. sinica (Z115) formed a group by themselves. The Results of UPGMA cluster showed that some accessions of Z. japonica, Z. sinica and Z. matrella clustered into one group: no one species clustered into one group, Moreover, a very few groups contained only one accession, suggesting that it had a special genetic base. In this paper, the diversity and relationships among 96 germplasms have been investigated and a preliminary discussion made on the ownership of part accessions. This research should provide a scientific basis at the molecular level for further study and the application of Zoysia grass germplasms.