Genetic diversity of Dichondra repens germplasm in southwest China by SRAP

Abstract

Abstract: The genetic diversity of 21 Dichondra repens, were analyzed using 19 highly polymorphic and stable primer pairs selected from 80 pairs of SRAP primers and the 19 primer pairs produced 212 bands, including 156 polymorphic bands (8.2 bands per primer pair). The average percentage of polymorphic bands was 73.58%. Genetic similarity of D. repens was calculated by Nei’s method and showed that the genetic similarity ranged from 0.444-0.980, and the average Nei’s coefficient was 0.76. It suggested that there was a rich genetic diversity among the resources of D. repens tested. The shape of the SRAP dendrogram showed that the 21 materials could be divided into three groups with 0.78 thresholds, and materials from the same origin frequently clustered into the same group. The research indicated a correlation among the wild resources and their geographical and ecological environments.

CLC Number:

Q943

FU Wei, GAN You-min, WU Jia-hai, WANG Xiao-li, CHEN Wei, ZHANG Jian-bo. Genetic diversity of Dichondra repens germplasm in southwest China by SRAP[J]. Acta Prataculturae Sinica, 2010, 19(6): 164-170.

References

[1] 中国科学院植物研究所. 中国高等植物图鉴(第三册)[M]. 北京: 科学出版社, 1983.
[2] 中国科学院中国植物志编辑委员会. 中国植物志(第64卷, 第1分册)[M]. 北京: 科学出版社, 1979.
[3] Pereira J A, Quintana R D, Monge S. Diets of plains vizcacha, greater rhea and cattle in Argentina[J]. Journal of Range Management, 2003, 56: 1, 13-20.
[4] 刘光明, 彭友林, 宋泽运. 马蹄金的形态组织鉴定[J]. 常德师范学院学报(自然科学版), 2001, 13(1): 73, 76-77.
[5] 谢彩云, 尚以顺. 优良草坪植物马蹄金的生物学特性及栽培管理技术[J]. 贵州农业科学, 2001, 29(1): 51-52.
[6] 黄必志.马蹄金草坪的建植与养护技术[J]. 草原与草坪, 2000, 3(1): 41-42.
[7] Brede A D, Sun S. Diveristy of turfgrass germp las in the Asian Pacific rim count ries and potential for reducing genetic vulnerability[J]. Crop Science, 1995, 35: 317-321.
[8] Li G, Quiros C F. Sequence-related amplified polymorphism(SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica[J]. Theoretical and Applied Genetics, 2001, 103: 455-461.
[9] 李慧芝, 尹燕萍, 张春庆, 等. SRAP在葱栽培品种遗传多样性研究中的适用性分析[J]. 园艺学报, 2007, 34(4): 929-934.
[10] 昝逢刚, 吴转娣, 曾淇, 等. 荔枝种质遗传多样性的SRAP分析[J]. 分子植物育种, 2009, 7(3): 562-568.
[11] 张西西, 徐进, 王涛, 等. 万寿菊杂交一代遗传多态性的SRAP标记分析[J]. 园艺学报, 2008, 35(8): 1221-1226.
[12] Li G, Gao M, Yang B, et al. Gene for alignment between the Brassica and Arobidopsis genomes by direct transcriptome mapping[J]. Theoretical and Applied Genetics, 2003, 107: 168-180.
[13] 缪体云, 薄天岳, 陈锦秀. 甘蓝种质资源遗传多样性的SRAP分析[J]. 分子植物育种, 2010, 8(1): 94-98.
[14] 郑轶琦, 刘建秀. 草坪草分子遗传图谱的构建与应用研究进展[J]. 草业学报, 2009, 18(1): 155-162.
[15] 海燕, 何宁, 康明辉, 等. 新型分子标记SRAP及其应用[J]. 河南农业科学, 2006, (9): 9-12.
[16] Ferriol M, Pico B, Nuez F. Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers[J]. Theoretical and Applied Genetics, 2003, 107: 271-283.
[17] 郭海林, 刘建秀, 高鹤, 等. 结缕草属优良品系SSR指纹图谱的构建[J]. 草业学报, 2007, 16(2): 53-59.
[18] 郭海林, 郑轶琦, 陈宣, 等. 结缕草属植物种间关系和遗传多样的SRAP标记分析[J]. 草业学报, 2009, 18(5): 201-210.
[19] 薛丹丹, 郭海林, 郑轶琦, 等. 结缕草属植物杂交后代杂种真实性鉴定——SRAP分子标记[J]. 草业学报, 2009, 18(1): 72-79.
[20] 陈宣, 郭海林, 薛丹丹, 等. 结缕草属植物耐盐性SRAP分子标记研究[J]. 草业学报, 2009, 18(2): 66-75.
[21] 季杨, 张新全, 马啸, 等. 多花黑麦草品种(系)间杂交及其杂种后代SRAP遗传分析[J]. 草业学报, 2009, 18(4): 260-265.
[22] Budak H, Shearrman R C, Parmaksiz I, et al. Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism makers[J]. Theoretical and Applied Genetics, 2004, 108: 328-334.
[23] Budak H, Shearman R C, Gaussoin R E, et al. Application of sequence-related amplified polymorphism makers for character2ization of turf grass species[J]. Hortscience, 2004, 39(5): 955-958.
[24] Budak H, Shearman R C, Parmaksiz I, et al. Comparative analysis of seeded and vegetative biotype buffalograsses based on phytogenetic relationship using ISSRs, SSRs, RAPDs and SRAP[J]. Theoretical and Applied Genetics, 2004, 109: 280-288.
[25] 王志勇, 袁学军, 刘建秀, 等. 狗牙根SRAP-PCR反应体系优化及引物筛选[J]. 草业学报, 2008, 17(3): 79-85.
[26] 易杨杰, 张新全, 黄琳凯, 等. 野生狗牙根种质遗传多样性的SRAP研究[J]. 遗传, 2008, 30(1): 94-100.
[27] 曾兵. 鸭茅种质资源遗传多样性的分子标记及优异种质评价[D].雅安: 四川农业大学, 2007.
[28] 萧运峰, 高洁. 草坪植物——马蹄金的研究[J]. 四川草原, 1997, 2(4): 43-45.
[29] 陈燕, 刘忠义, 干友民, 等. 野生马蹄金无性系构件种群组成及生物量结构变异性[J]. 江西农业学报, 2008, 20(2): 23-25.
[30] 古长标, 郭成宝, 唐泉, 等. 南京地区马蹄金草坪草的引种与利用[J]. 江苏农业科学, 2000, 2: 58-59.
[31] 田志宏, 严寒, 许本波, 等. 马蹄金下胚轴离培养与植株再生[J]. 湖北农学院学报, 2002, 22(6): 497-500.
[32] 陈万胜, 王元英, 罗成刚, 等.利用正交设计优化烟草SRAP反应体系[J]. 分子植物育种, 2008, 6(1): 177-182.
[33] Rohlf F J. NTSYS-pc, Numerical Taxonomy and Multivariate Analysis System (Version 2.1)[M]. New York: Exeter Software, 2001.
[34] 王昆蕾, 干友民, 费凌, 等. 西南区野生马蹄金居群生态特性及形态变异[J]. 湖北农业科学, 2006, 45(6): 798-798.
[35] 王昆蕾. 西南区野生马蹄金遗传多样性研究[D]. 雅安: 四川农业大学, 2007.
[36] 陈智华, 苗佳敏, 钟金城, 等. 野生垂穗披碱草种质遗传多样性的SRAP研究[J]. 草业学报, 2009, 18(5): 192-200.