Effects of combinations of microorganisms on lactic acid production using fermentation straw

Abstract

Abstract: This study investigated the production of ligninase and L-lactic acid from mixed fermentation with Trichoderma reesei, Coprinus comatus, and Rhizopus oryzae. Under optimal conditions with an inoculation proportion of C. comatus to T. reesei of 5∶2, a time interval of 12 h, shake rotation speed of 150 r/min, after inocubation for 3 d at 26℃, the laccase activity reached 3 267.1 U/mL, an increase of 106% compared to single cultures. The agricultural wastes were treated for 84 h with crude enzymes extracted from the mixed fermentation of T. reesei and C.comatus at pH 5.0, 50℃ with a shaking speed 120 r/min: A 55.2% hydrolysis rate was obtained. The hydrolysed sugar was transformed into L-lactic acid by R. oryzae. Under optimal conditions, 3.36 g/L L-lactic acid was produced and the sugar conversion rate reached 67.40%.

CLC Number:

S816.6

XU Juan-juan, GE Chun-mei, SUN Qin-ying, ZHANG-Jie, FAN Mei-zhen, PAN ren-rui. Effects of combinations of microorganisms on lactic acid production using fermentation straw[J]. Acta Prataculturae Sinica, 2010, 19(6): 120-125.

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