Abstract: SRAP (sequence-related amplified polymorphism) markers were used to analyze genetic diversity of 41 white clover (Trifolium repens) accessions using 20 pairs of random primers. The results were: 1) The primer pairs amplified 479 clear bands, including 411 polymorphic bands, which ranged from 13 to 44 bands per primer pair with an average of 23.95 bands. The percent of polymorphic loci was 85.16%, and the polymorphism information content ranged from 0.182 to 0.334 with an average of 0.268, which showed that the tested white clover had a rich genetic diversity of germplasm. 2) Genetic similarity ranged from 0.586 6 to 0.989 6 among all accessions with an average of 0.730 7, which also suggested that there was a rich genetic variation among the white clover germplasms. 3) Cluster analysis and principal component analysis of 41 white clover accessions divided them into 3 groups on the genetic similarity of 0.71 and most of the accessions from the same or similar ecologic geographical environment clustered in one group, showing a certain degree of eco-geographical distribution.