Genetic diversity of Cichorium intybus germplasm resources using sequence-related amplified polymorphism (SRAP) molecular markers

Abstract

Abstract: Genetic diversity of 48 accessions of all Cichorium intybus collected from 5 geographical groups was analyzed using sequence-related amplified polymorphism (SRAP) molecular markers with 28 SRAP primer pairs. 1) A total of 333 alleles were detected at 28 loci, an average of 11.89. Occurrence of polymorphic sites (P) was 95.09% and the polymorphic information content (PIC) ranged from 0.23(me3+em3)to 0.44(me1+em10), an average of 0.33. 2) The genetic similarity (GS) among all accessions ranged from 0.55 to 0.89 with an average of 0.69. For all geographical groups, the GS value ranged from 0.63 to 0.96. Among these groups, a high genetic diversity was observed in groups from the Netherlands (P, 95.52%) and Italy (P, 95.38%). These results suggested that there was a high genetic diversity among all C. intybus accessions tested. 3) Based on cluster and principal component analyses, 48 accessions could be divided into five groups by the nearest phylogenetic relationship, and the five groups reflected the five geographical origins.

CLC Number:

Q943
S540.24

LUO Yan, BAI Shi-qie, PENG Yan, ZHANG Yu, MA Xiao. Genetic diversity of Cichorium intybus germplasm resources using sequence-related amplified polymorphism (SRAP) molecular markers[J]. Acta Prataculturae Sinica, 2010, 19(5): 139-147.

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