Analysis of genetic diversity in Salix oritrepha from different altitudes in Gansu Qilian Mountains using SSR markers

Abstract

Abstract: Genetic diversity and genetic structure of Salix oritrepha (about 180 individuals in total) from nine altitudes on the Qilian mountains were analyzed by SSR (simple sequence repeat) markers. Fifteen highly stable and repeatable SSRs (selected from 47 SSRs of Populus) detected a total of 211 alleles of which 176 (85%) were polymorphic. At the species level, the Shannon index (I) was 0.419 2, Nei’s gene diversity index (H) was 0.279 6, the coefficient of gene differentiation (Gst) was 0.093 5, and the gene flow (Nm) was 4.848 7. There was more genetic differentiation within altitudes than between altitudes. All of the results include P, H, and I showed that S. oritrepha has a high genetic diversity in the Gansu Qilian mountain. Cluster analysis showed a direct correlation between genetic distance and geographic distance. Genetic diversity of S. oritrepha was notably affected by the latitude and altitude: the higher the altitude gradient, the more abundant the genetic diversity of S. oritrepha.

CLC Number:

GUO Min, MA Yan-jun, LI Yi. Analysis of genetic diversity in Salix oritrepha from different altitudes in Gansu Qilian Mountains using SSR markers[J]. Acta Prataculturae Sinica, 2012, 21(5): 114-121.

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