The genetic diversity of three ecological Medicago ruthenica germplasms revealed by ISSR and SSR

Abstract

Abstract: Quantitative and classification was based on germplasm collection and utilization. The genetic diversity of 14 wild Medicago ruthenica was assessed by screening with 18 pairs of SSR and 18 ISSR primers. SSR primers amplified 109 clear polymorphic bands, and the polymorphism rate was 80.09% with a similarity coefficient ranging from 0.669 to 0.885. ISSR primers amplified 125 polymorphic bands and the polymorphism ratio was 87.08% with a similarity coefficient ranging from 0.448 to 0.811. UPGMA (Unweighted Pair Group Method with Arithmetic mean) cluster analysis based on two markers showed that part of the M. ruthenica material grouped into one group. Mantel tests showed that the genetic similarity of two markers had a significant correlation (r=0.019 6, t=0.121 2). Compared with yellow M. ruthenica genetic diversity levels, those of M. ruthenica were higher. The genetic difference was highest in M. ruthenica var. oblongifolia.

CLC Number:

Q943

LI Hong-yan, LI Zhi-yong, SHI Wen-gui, CAI Li-yan, ZHANG Jing-ping. The genetic diversity of three ecological Medicago ruthenica germplasms revealed by ISSR and SSR[J]. Acta Prataculturae Sinica, 2012, 21(5): 107-113.

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