Abstract: Mature embryos of bentgrass (Agrostis stolonifera) Penn A-1 were used to establish a highly efficient regeneration system. The dual genes Lyz-GFP (Lysozyme gene and green fluorescence protein gene) were transformed into embryonic calli of creeping bentgrass by an agrobacterium-mediation method to obtain transgenic plants with disease resistance capability. The best conditions for transformation of the Lyz-GFP genes in Penn A-1 were studied. The callus induction ratio of Penn A-1 was highest at 36% on MS medium supplemented with 2.0 mg/L 2, 4-D and 0.1 mg/L 6-BA, and the calli had the optimum growth status. The highest differentiation ratio was 42.5% on MSO medium supplemented with 0.5 mg/L NAA. The study also showed that growth of agrobacterium LBA4404 was restrained by 300 mg/L cefotaxime (Cef). Transformed calli performed well in later growth periods as well as in the regeneration of transgenic plants after infection with agrobacterium LBA4404 (OD₆₀₀: 0.3-0.5) carried with pBI121-Lyz-GFP for 10-15 min, and co-cultured for 3 days. The transformation ratio of calli was 12.5%; the regeneration ratio of transgenic plants was 27.5%; the transgenic plants strongly expressed fluorescence, and the 750 bp target fragments of the GFP gene were amplified by PCR from 2 acquired transgenic plants with expression of Penn A-1 fluorescence.