Effects of excess manganese toxicity on growth and antioxidant enzyme activity in Stylosanthes guianensis seedlings

doi:10.11686/cyxb2017441

Abstract

Abstract: The aim of this study was to investigate the potential mechanisms underlying stylo (Stylosanthes guianensis) tolerance to high Mn levels. The growth responses and Mn tolerance in stylo were assessed in two stylo genotypes, TF210 and TF226 grown hydroponically. Plant dry weight, root growth parameters, chlorophyll concentration (soil plant analyzer development, SPAD), maximum photochemical efficiency (F_v/F_m) as well as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities were measured under two Mn levels. Results showed that significant decreases in shoots and roots growth, SPAD and F_v/F_m values were observed in stylo genotype TF210 compared to TF226 under 400 μmol·L^-1 MnSO₄ treatment, suggesting that TF226 was a more Mn-tolerant genotype compared to TF210. Although Mn content in shoots and roots of both genotypes was increased by high Mn treatment, Mn content in TF226 was lower than that in TF210, suggesting that less Mn was accumulated in TF226 compared to TF210. Furthermore, SOD, POD and CAT activities in leaves of TF226 were significantly increased under high Mn treatment, while only SOD activity in leaves of TF210 was increased by high Mn. Activities of the three tested enzymes in TF226 were higher than those in TF210 under high Mn. These results suggest that regulations of root growth, Mn accumulation and antioxidant enzyme activities might be the adaptive mechanisms of stylo tolerance to Mn toxicity. The present study not only provides theoretical basis for dissecting Mn tolerant mechanisms, but also providing germplasm resources for improving adaptation to Mn toxicity in stylo.

Key words: Stylosanthes, Mn toxicity, root growth, antioxidant enzyme

LIU Pan-dao, LUO Jia-jia, BAI Chang-jun, CHEN Zhi-jian, LIU Guo-dao. Effects of excess manganese toxicity on growth and antioxidant enzyme activity in Stylosanthes guianensis seedlings[J]. Acta Prataculturae Sinica, 2018, 27(9): 194-200.

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