Effect of nitrogen addition on soil bacterial diversity in alpine degraded grasslands of differing slope

doi:10.11686/cyxb2020109

Abstract

Abstract:

The aim of this study was to determine the effects of nitrogen addition on the bacterial community in soils in degraded alpine meadows with different slopes in Guoluo Prefecture， in the Sanjiangyuan Region. Degraded grasslands with different slopes were supplemented with nitrogen at three levels： low nitrogen （LN， 2 g N·m^-2）， medium nitrogen （MN， 5 g N·m^-2）， high nitrogen （HN， 10 g N·m^-2）， and the effects on soil bacterial diversity were determined. The 16s rDNA region of soil bacteria was sequenced and analyzed using Miseq PE250 sequencing technology. Actinomycota and Proteobacteria were the two main bacterial classes in the grassland soil in the study area. Individual phyla with extremely low abundance in soil and those with high abundance （>61.4%）， including unidentified bacterial genera， differed significantly between gentle slope and steep slope regions （P<0.05）. Different nitrogen addition levels did not significantly effect on the relative abundance of bacterial phyla and most bacterial genera in degraded alpine grassland， but had significant effects on some less abundant genera （P<0.05）. On both gentle and steep slopes， the overall soil microbial richness and diversity tended to initially decrease and then increase with increasing nitrogen addition. The HN treatment inhibited the decrease of soil bacterial richness and diversity. The effect of nitrogen addition on bacterial diversity was threshold-limited. Among the three treatments， the MN treatment significantly reduced soil bacterial richness and diversity （P<0.05）.

Key words: grassland degradation, nitrogen addition, bacterial diversity, high-throughput sequencing

Cheng-yi LI, Xi-lai LI, Yuan-wu YANG, Hong-lin LI, De-fei LIANG. Effect of nitrogen addition on soil bacterial diversity in alpine degraded grasslands of differing slope[J]. Acta Prataculturae Sinica, 2020, 29(12): 161-170.

Figures/Tables 8

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坡度 Slope	细菌属 Genera of bacteria	P值 P-value
坡度 Slope	细菌属 Genera of bacteria	CK/LN	CK/MN	CK/HN	LN/MN	LN/HN	MN/HN
缓坡 Gentle slope	伯克氏菌属 Chitinimonas	1.000	0.002	1.000	0.002	1.000	0.002
	杆菌属 Lacibacter	0.143	0.007	1.000	0.227	0.143	0.007
	产碱菌属 Azohydromonas	0.034	0.233	0.003	0.351	0.412	0.080
	酸杆菌属 Acidibacter	0.003	0.193	0.087	0.102	0.220	0.683
	生丝微菌属 Hyphomicrobium	0.316	0.504	0.005	0.738	0.074	0.034
	斯科曼氏菌属 Williamsia	0.065	0.282	0.443	0.443	0.009	0.065
	泉发菌属 Terrimonas	0.049	0.655	0.110	0.016	0.710	0.041
陡坡 Steep slope	发酵单胞菌属 Zymomonas	0.494	0.003	1.000	0.022	0.494	0.003
	鼎湖杆菌属 Dinghuibacter	0.192	0.004	1.000	0.117	0.192	0.004
	立克次体属 Rickettsia	0.009	0.009	0.009	1.000	1.000	1.000
	黄杆菌属 Luteolibacter	0.338	0.211	0.061	0.027	0.005	0.532
	根霉菌属 Rhizomicrobium	0.128	0.206	0.911	0.005	0.102	0.249
	微酸菌属 Ilumatobacter	0.019	0.434	0.017	0.118	0.970	0.109
	玫瑰螺旋菌属 Skermanella	0.334	0.193	0.147	0.023	0.016	0.882

坡度 Slope	细菌属 Genera of bacteria	P值 P-value
坡度 Slope	细菌属 Genera of bacteria	CK/LN	CK/MN	CK/HN	LN/MN	LN/HN	MN/HN
缓坡 Gentle slope	伯克氏菌属 Chitinimonas	1.000	0.002	1.000	0.002	1.000	0.002
	杆菌属 Lacibacter	0.143	0.007	1.000	0.227	0.143	0.007
	产碱菌属 Azohydromonas	0.034	0.233	0.003	0.351	0.412	0.080
	酸杆菌属 Acidibacter	0.003	0.193	0.087	0.102	0.220	0.683
	生丝微菌属 Hyphomicrobium	0.316	0.504	0.005	0.738	0.074	0.034
	斯科曼氏菌属 Williamsia	0.065	0.282	0.443	0.443	0.009	0.065
	泉发菌属 Terrimonas	0.049	0.655	0.110	0.016	0.710	0.041
陡坡 Steep slope	发酵单胞菌属 Zymomonas	0.494	0.003	1.000	0.022	0.494	0.003
	鼎湖杆菌属 Dinghuibacter	0.192	0.004	1.000	0.117	0.192	0.004
	立克次体属 Rickettsia	0.009	0.009	0.009	1.000	1.000	1.000
	黄杆菌属 Luteolibacter	0.338	0.211	0.061	0.027	0.005	0.532
	根霉菌属 Rhizomicrobium	0.128	0.206	0.911	0.005	0.102	0.249
	微酸菌属 Ilumatobacter	0.019	0.434	0.017	0.118	0.970	0.109
	玫瑰螺旋菌属 Skermanella	0.334	0.193	0.147	0.023	0.016	0.882

坡度 Slope	处理 Treatment	Chao1指数 Chao1 index	观测深度 Goods_coverage	观测物种数 Observed_species	Shannon指数 Shannon index
缓坡Gentle slope	CK	3220±68Ab	0.968±0.001Aa	2460±25Aa	9.41±0.16Ab
	LN	3158±121ABa	0.968±0.002Aa	2412±80Ab	9.34±0.07ABb
	MN	2990±181Bb	0.970±0.002Aa	2266±135Bb	9.23±0.12Bb
	HN	3170±19ABa	0.968±0.001Aa	2397±61Aa	9.36±0.10ABb
陡坡Steep slope	CK	3490±178Aa	0.962±0.002Ab	2594±130Aa	9.46±0.08Aa
	LN	3479±63Aa	0.963±0.001Ab	2539±54Aa	9.39±0.14Aa
	MN	3323±75Ba	0.962±0.001Ab	2568±78Aa	9.30±0.19Ba
	HN	3405±250Aa	0.963±0.003Ab	2492±147Aa	9.44±0.06Aa

坡度 Slope	处理 Treatment	Chao1指数 Chao1 index	观测深度 Goods_coverage	观测物种数 Observed_species	Shannon指数 Shannon index
缓坡Gentle slope	CK	3220±68Ab	0.968±0.001Aa	2460±25Aa	9.41±0.16Ab
	LN	3158±121ABa	0.968±0.002Aa	2412±80Ab	9.34±0.07ABb
	MN	2990±181Bb	0.970±0.002Aa	2266±135Bb	9.23±0.12Bb
	HN	3170±19ABa	0.968±0.001Aa	2397±61Aa	9.36±0.10ABb
陡坡Steep slope	CK	3490±178Aa	0.962±0.002Ab	2594±130Aa	9.46±0.08Aa
	LN	3479±63Aa	0.963±0.001Ab	2539±54Aa	9.39±0.14Aa
	MN	3323±75Ba	0.962±0.001Ab	2568±78Aa	9.30±0.19Ba
	HN	3405±250Aa	0.963±0.003Ab	2492±147Aa	9.44±0.06Aa

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