Acta Prataculturae Sinica ›› 2022, Vol. 31 ›› Issue (5): 135-143.DOI: 10.11686/cyxb2021097
Qing ZHANG(), Jing XING, Jia-ming YAO, Ting-chao YIN, Xin-ru HUANG, Yue HE, Jing ZHANG(), Bin XU
Received:
2021-03-16
Revised:
2021-03-29
Online:
2022-05-20
Published:
2022-03-30
Contact:
Jing ZHANG
Qing ZHANG, Jing XING, Jia-ming YAO, Ting-chao YIN, Xin-ru HUANG, Yue HE, Jing ZHANG, Bin XU. The role of a cytokinin signaling pathway type-B ARR transcription factor, LpARR10, in cadmium tolerance of perennial ryegrass[J]. Acta Prataculturae Sinica, 2022, 31(5): 135-143.
引物名称Primer | 上游引物序列Forward primer sequence (5′-3′) | 下游引物序列Reverse primer sequence (5′-3′) |
---|---|---|
LpARR10-CDS | ATTTggatccATGCGCGTGCTCGCCGTC | ATTTactagtGACCAGCTGCCAGTCCCCAT |
LpARR10-qRT | GATGTCGGGAATACGGGCGG | GAGCCCCCTTTGCAGCCTAC |
AtTUBULIN4-qRT | CTGTTTCCGTACCCTCAAGC | AGGGAAACGAAGACAGCAAG |
Bar gene | TCAAATCTCGGTGACGGGCAGGAC | AACCGCAGGAGTGGACGGACGA |
Table 1 Primers used in the study
引物名称Primer | 上游引物序列Forward primer sequence (5′-3′) | 下游引物序列Reverse primer sequence (5′-3′) |
---|---|---|
LpARR10-CDS | ATTTggatccATGCGCGTGCTCGCCGTC | ATTTactagtGACCAGCTGCCAGTCCCCAT |
LpARR10-qRT | GATGTCGGGAATACGGGCGG | GAGCCCCCTTTGCAGCCTAC |
AtTUBULIN4-qRT | CTGTTTCCGTACCCTCAAGC | AGGGAAACGAAGACAGCAAG |
Bar gene | TCAAATCTCGGTGACGGGCAGGAC | AACCGCAGGAGTGGACGGACGA |
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