Effects of enclosure on soil microbial carbon source utilization characteristics of sagebrush desert grassland

doi:10.11686/cyxb2024071

Abstract

Abstract:

This study was conducted in Hutubi and Manas Counties of Changji Hui Autonomous Prefecture in Xinjiang Uygur Autonomous Region， and investigated how soil microbial carbon source utilization patterns in sagebrush desert grassland changed in response to fencing enclosure. Field investigation and laboratory analyses were combined to measure and analyze the characteristics of soil microbial carbon source utilization both inside and outside the enclosure. Factors driving responses were examined based on vegetation characteristics and physicochemical properties of the soil. The results showed： 1） After fencing enclosure， the aboveground biomass （AGB） and litter biomass （LB） of sagebrush desert grassland were significantly increased by 142.53%-250.05% and 135.68%-259.84% （P<0.05）， respectively. The contents of soil organic carbon （SOC）， total nitrogen （TN） and total phosphorus （TP） in 0-10 cm and 30-50 cm soil layers were increased by 4.88%-35.33%， 10.48%-46.58% and 4.48%-21.88%， respectively， and the contents of total nitrogen and total phosphorus also showed significant changes （P<0.05）. 2） The utilization by soil microbial communities of six categories of carbon source increased significantly after enclosure， and the behavior of surface and deeper soil layers was consistent （P>0.05）. 3） After enclosure， the McIntosh index and Shannon-Wiener index increased by 14.72%-47.01% and 36.29%-74.76%， respectively， while the Simpson index decreased by 1.09%-7.53% （P>0.05）， except in the 0-10 cm soil horizon in Hutubi County. Enclosure improved the microbial diversity of sagebrush desert grassland. 4） Through redundancy analysis， it was found that the main driving factors of microbial carbon source in the 0-10 cm soil layer were SOC and litter biomass， while the driving factors in the 30-50 cm soil layer were soil TP and aboveground biomass. In conclusion， the enclosure by fencing in sagebrush desert grassland was found to enhance the metabolic activity of soil microorganisms， thereby fostering nutrient cycling and transformation within the ecosystem， specifically in terms of SOC， TN and TP. Furthermore， this practice led to an improvement in microbial diversity， ultimately promoting the restoration of degraded grassland and enhancing the overall health and resilience of the sagebrush desert grassland ecosystem.

Key words: enclosure, sagebrush desert grassland, metabolic activity of carbon sources, microbial carbon source diversity, Biolog-ECO

ASITAIKEN·Julihaiti, Zong-jiu SUN, Bing-jie YU, DIDAER·Bisulidan, Mei-sha LI, Yi-sheng JING. Effects of enclosure on soil microbial carbon source utilization characteristics of sagebrush desert grassland[J]. Acta Prataculturae Sinica, 2025, 34(1): 29-40.

Figures/Tables 7

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项目 Item	指标 Index	土层 Soil layer （cm）	呼图壁Hutubi		玛纳斯Manas
项目 Item	指标 Index	土层 Soil layer （cm）	放牧Freely grazing	封育Grazing exclusion	放牧Freely grazing	封育Grazing exclusion
生物量 Biomass （g·m^-2）	地上Aboveground	-	82.29±10.08b	199.58±14.62a	64.30±10.66b	225.08±19.20a
	凋落物Litter	-	9.91±3.06b	35.66±3.62a	9.39±1.54b	22.13±1.90a
	地下Belowground	0~10	164.45±72.15a	206.50±30.95a	253.42±149.84a	107.00±54.22a
	地下Belowground	30~50	22.40±5.54b	98.25±29.03a	54.17±6.50a	23.83±10.13a
土壤理化性质 Soil physicochemical properties	酸碱度pH	0~10	7.96±0.04a	7.91±0.10a	8.39±0.01a	8.32±0.09a
	酸碱度pH	30~50	8.28±0.16a	8.27±0.16a	8.39±0.10a	8.59±0.16a
	容重Bulk density （g·cm^-3）	0~10	1.31±0.02a	1.31±0.03a	1.16±0.03a	1.24±0.04a
	容重Bulk density （g·cm^-3）	30~50	1.40±0.09a	1.42±0.04a	1.25±0.09a	1.29±0.03a
	含水量Water content （%）	0~10	4.62±1.05a	3.08±1.08a	1.97±0.50a	2.95±0.60a
	含水量Water content （%）	30~50	8.23±2.09a	5.66±0.93a	6.31±1.18a	5.13±0.95a
	有机碳Organic carbon （g·kg^-1）	0~10	15.78±1.01a	16.55±1.45a	12.34±2.60a	16.70±1.47a
	有机碳Organic carbon （g·kg^-1）	30~50	8.05±0.58a	9.31±1.48a	6.59±1.06a	7.85±0.71a
	全氮Total nitrogen （g·kg^-1）	0~10	2.76±0.07b	3.15±0.07a	2.34±0.09b	3.43±0.13a
	全氮Total nitrogen （g·kg^-1）	30~50	2.23±0.08b	2.60±0.09a	2.10±0.20a	2.32±0.23a
	全磷Total phosphorus （g·kg^-1）	0~10	0.73±0.02a	0.78±0.03a	0.77±0.01b	0.87±0.03a
	全磷Total phosphorus （g·kg^-1）	30~50	0.67±0.03a	0.70±0.03a	0.64±0.08a	0.78±0.02a

项目 Item	指标 Index	土层 Soil layer （cm）	呼图壁Hutubi		玛纳斯Manas
项目 Item	指标 Index	土层 Soil layer （cm）	放牧Freely grazing	封育Grazing exclusion	放牧Freely grazing	封育Grazing exclusion
生物量 Biomass （g·m^-2）	地上Aboveground	-	82.29±10.08b	199.58±14.62a	64.30±10.66b	225.08±19.20a
	凋落物Litter	-	9.91±3.06b	35.66±3.62a	9.39±1.54b	22.13±1.90a
	地下Belowground	0~10	164.45±72.15a	206.50±30.95a	253.42±149.84a	107.00±54.22a
	地下Belowground	30~50	22.40±5.54b	98.25±29.03a	54.17±6.50a	23.83±10.13a
土壤理化性质 Soil physicochemical properties	酸碱度pH	0~10	7.96±0.04a	7.91±0.10a	8.39±0.01a	8.32±0.09a
	酸碱度pH	30~50	8.28±0.16a	8.27±0.16a	8.39±0.10a	8.59±0.16a
	容重Bulk density （g·cm^-3）	0~10	1.31±0.02a	1.31±0.03a	1.16±0.03a	1.24±0.04a
	容重Bulk density （g·cm^-3）	30~50	1.40±0.09a	1.42±0.04a	1.25±0.09a	1.29±0.03a
	含水量Water content （%）	0~10	4.62±1.05a	3.08±1.08a	1.97±0.50a	2.95±0.60a
	含水量Water content （%）	30~50	8.23±2.09a	5.66±0.93a	6.31±1.18a	5.13±0.95a
	有机碳Organic carbon （g·kg^-1）	0~10	15.78±1.01a	16.55±1.45a	12.34±2.60a	16.70±1.47a
	有机碳Organic carbon （g·kg^-1）	30~50	8.05±0.58a	9.31±1.48a	6.59±1.06a	7.85±0.71a
	全氮Total nitrogen （g·kg^-1）	0~10	2.76±0.07b	3.15±0.07a	2.34±0.09b	3.43±0.13a
	全氮Total nitrogen （g·kg^-1）	30~50	2.23±0.08b	2.60±0.09a	2.10±0.20a	2.32±0.23a
	全磷Total phosphorus （g·kg^-1）	0~10	0.73±0.02a	0.78±0.03a	0.77±0.01b	0.87±0.03a
	全磷Total phosphorus （g·kg^-1）	30~50	0.67±0.03a	0.70±0.03a	0.64±0.08a	0.78±0.02a

项目 Item	指标 Index	土层 Soil layer （cm）	解释率Explanation rate （%）		显著性Significance （P）
项目 Item	指标 Index	土层 Soil layer （cm）	放牧 Freely grazing	封育 Grazing exclusion	放牧 Freely grazing	封育 Grazing exclusion
生物量 Biomass	地上Aboveground	0~10	17.0	22.1	0.254	0.168
	地上Aboveground	30~50	8.2	14.0	0.686	0.356
	凋落物Litter	0~10	12.1	20.5	0.592	0.204
	凋落物Litter	30~50	8.2	2.7	0.556	0.954
	地下Belowground	0~10	22.1	2.6	0.208	0.996
	地下Belowground	30~50	11.9	6.3	0.456	0.740
土壤理化性质 Soil physicochemical properties	酸碱度pH	0~10	6.2	27.4	0.908	0.070
	酸碱度pH	30~50	10.4	6.5	0.548	0.714
	容重Bulk density	0~10	8.2	18.3	0.816	0.250
	容重Bulk density	30~50	32.0	8.9	0.062	0.656
	含水量Water content	0~10	18.0	9.0	0.272	0.716
	含水量Water content	30~50	3.2	3.8	0.972	0.910
	有机碳Organic carbon	0~10	17.5	16.4	0.256	0.296
	有机碳Organic carbon	30~50	4.6	7.1	0.908	0.710
	全氮Total nitrogen	0~10	9.0	10.5	0.746	0.678
	全氮Total nitrogen	30~50	13.2	8.2	0.424	0.652
	全磷Total phosphorus	0~10	11.6	15.7	0.588	0.398
	全磷Total phosphorus	30~50	13.3	25.6	0.446	0.182

项目 Item	指标 Index	土层 Soil layer （cm）	解释率Explanation rate （%）		显著性Significance （P）
项目 Item	指标 Index	土层 Soil layer （cm）	放牧 Freely grazing	封育 Grazing exclusion	放牧 Freely grazing	封育 Grazing exclusion
生物量 Biomass	地上Aboveground	0~10	17.0	22.1	0.254	0.168
	地上Aboveground	30~50	8.2	14.0	0.686	0.356
	凋落物Litter	0~10	12.1	20.5	0.592	0.204
	凋落物Litter	30~50	8.2	2.7	0.556	0.954
	地下Belowground	0~10	22.1	2.6	0.208	0.996
	地下Belowground	30~50	11.9	6.3	0.456	0.740
土壤理化性质 Soil physicochemical properties	酸碱度pH	0~10	6.2	27.4	0.908	0.070
	酸碱度pH	30~50	10.4	6.5	0.548	0.714
	容重Bulk density	0~10	8.2	18.3	0.816	0.250
	容重Bulk density	30~50	32.0	8.9	0.062	0.656
	含水量Water content	0~10	18.0	9.0	0.272	0.716
	含水量Water content	30~50	3.2	3.8	0.972	0.910
	有机碳Organic carbon	0~10	17.5	16.4	0.256	0.296
	有机碳Organic carbon	30~50	4.6	7.1	0.908	0.710
	全氮Total nitrogen	0~10	9.0	10.5	0.746	0.678
	全氮Total nitrogen	30~50	13.2	8.2	0.424	0.652
	全磷Total phosphorus	0~10	11.6	15.7	0.588	0.398
	全磷Total phosphorus	30~50	13.3	25.6	0.446	0.182

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