Acta Prataculturae Sinica ›› 2026, Vol. 35 ›› Issue (2): 68-82.DOI: 10.11686/cyxb2025081
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Ji-yuan ZHANG1(
), Hai-quan AN2, Jing-yi PAN1, Chang LIU1, Si-si LONG1, Li-li ZHAO1(
)
Received:2025-03-11
Revised:2025-04-29
Online:2026-02-20
Published:2025-12-24
Contact:
Li-li ZHAO
Ji-yuan ZHANG, Hai-quan AN, Jing-yi PAN, Chang LIU, Si-si LONG, Li-li ZHAO. Drought resistance evaluation of seed germination and seedling growth of seven alfalfa cultivars[J]. Acta Prataculturae Sinica, 2026, 35(2): 68-82.
品种 Cultivars | 千粒重Thousand seeds weight (g) | 来源 Source | 原产地 Place of origin |
|---|---|---|---|
| 皇冠Phabulous | 2.32 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 美国America |
| 角斗士Gladiator | 2.25 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 意大利Italy |
| PANGO | 2.25 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 美国America |
| 巨能801 Magnum 801 | 2.10 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 美国America |
| WL525 | 2.54 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 美国America |
| 维多利亚Victoria | 2.19 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 加拿大Canada |
| 迪特Dieter | 2.18 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 意大利Italy |
Table 1 Alfalfa cultivars tested
品种 Cultivars | 千粒重Thousand seeds weight (g) | 来源 Source | 原产地 Place of origin |
|---|---|---|---|
| 皇冠Phabulous | 2.32 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 美国America |
| 角斗士Gladiator | 2.25 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 意大利Italy |
| PANGO | 2.25 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 美国America |
| 巨能801 Magnum 801 | 2.10 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 美国America |
| WL525 | 2.54 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 美国America |
| 维多利亚Victoria | 2.19 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 加拿大Canada |
| 迪特Dieter | 2.18 | 贵州众智恒生态科技有限公司Guizhou Zhongzhiheng Ecological Technology Co., Ltd | 意大利Italy |
品种 Cultivars | PEG-6000浓度 PEG-6000 concentration (%) | 干重 Dry weight (g·plant-1) | 鲜重 Fresh weight (g·plant-1) | 相对含水量 Relative water content (%) |
|---|---|---|---|---|
皇冠 Phabulous | 0 | 0.0015±0.0001Bab | 0.0284±0.0022Aa | 94.72±0.0022Ab |
| 15 | 0.0017±0.0001Ab | 0.0242±0.0011Bbc | 92.88±0.0040Bc | |
角斗士 Gladiator | 0 | 0.0017±0.0003Aa | 0.0219±0.0036Bc | 92.26±0.0013Bd |
| 15 | 0.0017±0.0001Ab | 0.0297±0.0014Aa | 94.23±0.0017Ab | |
| PANGO | 0 | 0.0011±0.0001Bb | 0.0278±0.0030Aab | 95.68±0.0060Aa |
| 15 | 0.0022±0.0001Aa | 0.0232±0.0022Bc | 90.34±0.0085Be | |
巨能801 Magnum 801 | 0 | 0.0015±0.0001Aab | 0.0230±0.0021Ac | 93.38±0.0033Bc |
| 15 | 0.0012±0.0004Ac | 0.0272±0.0044Aab | 95.56±0.0091Aa | |
| WL525 | 0 | 0.0011±0.0002Bb | 0.0272±0.0021Aab | 95.64±0.0107Aa |
| 15 | 0.0019±0.0001Aab | 0.0225±0.0026Bc | 91.33±0.0126Bde | |
维多利亚 Victoria | 0 | 0.0016±0.0002Aa | 0.0238±0.0022Abc | 93.23±0.0060Bc |
| 15 | 0.0010±0.0000Bc | 0.0255±0.0010Abc | 95.86±0.0019Aa | |
迪特 Dieter | 0 | 0.0014±0.0002Aab | 0.0273±0.0019Aab | 94.54±0.0059Ab |
| 15 | 0.0013±0.0003Ac | 0.0164±0.0008Bd | 92.09±0.0143Bcd |
Table 2 Effects of drought stress on seedling biomass and relative water content of seven alfalfa cultivars
品种 Cultivars | PEG-6000浓度 PEG-6000 concentration (%) | 干重 Dry weight (g·plant-1) | 鲜重 Fresh weight (g·plant-1) | 相对含水量 Relative water content (%) |
|---|---|---|---|---|
皇冠 Phabulous | 0 | 0.0015±0.0001Bab | 0.0284±0.0022Aa | 94.72±0.0022Ab |
| 15 | 0.0017±0.0001Ab | 0.0242±0.0011Bbc | 92.88±0.0040Bc | |
角斗士 Gladiator | 0 | 0.0017±0.0003Aa | 0.0219±0.0036Bc | 92.26±0.0013Bd |
| 15 | 0.0017±0.0001Ab | 0.0297±0.0014Aa | 94.23±0.0017Ab | |
| PANGO | 0 | 0.0011±0.0001Bb | 0.0278±0.0030Aab | 95.68±0.0060Aa |
| 15 | 0.0022±0.0001Aa | 0.0232±0.0022Bc | 90.34±0.0085Be | |
巨能801 Magnum 801 | 0 | 0.0015±0.0001Aab | 0.0230±0.0021Ac | 93.38±0.0033Bc |
| 15 | 0.0012±0.0004Ac | 0.0272±0.0044Aab | 95.56±0.0091Aa | |
| WL525 | 0 | 0.0011±0.0002Bb | 0.0272±0.0021Aab | 95.64±0.0107Aa |
| 15 | 0.0019±0.0001Aab | 0.0225±0.0026Bc | 91.33±0.0126Bde | |
维多利亚 Victoria | 0 | 0.0016±0.0002Aa | 0.0238±0.0022Abc | 93.23±0.0060Bc |
| 15 | 0.0010±0.0000Bc | 0.0255±0.0010Abc | 95.86±0.0019Aa | |
迪特 Dieter | 0 | 0.0014±0.0002Aab | 0.0273±0.0019Aab | 94.54±0.0059Ab |
| 15 | 0.0013±0.0003Ac | 0.0164±0.0008Bd | 92.09±0.0143Bcd |
品种 Cultivars | PEG-6000浓度 PEG-6000 concentration (%) | 苗长 Seedling length (mm) | 芽长 Bud length (mm) | 根长 Root length (mm) | 芽根比 Bud root ratio |
|---|---|---|---|---|---|
皇冠 Phabulous | 0 | 28.74±3.20Bc | 18.37±2.07Aa | 10.37±1.63Bd | 1.79±0.28Aa |
| 15 | 46.33±4.12Aa | 15.04±1.22Bb | 31.28±3.30Aa | 0.48±0.04Bc | |
角斗士 Gladiator | 0 | 31.96±6.13Abc | 16.82±1.80Aa | 15.14±4.41Abcd | 1.15±0.23Abcd |
| 15 | 34.38±5.79Abc | 14.95±0.84Ab | 19.43±6.56Ab | 0.89±0.48Abc | |
| PANGO | 0 | 34.77±2.26Aabc | 18.70±1.24Aa | 16.07±2.74Abcd | 1.19±0.23Abc |
| 15 | 25.75±5.72Bd | 8.51±1.90Bc | 17.24±3.86Abc | 0.49±0.02Bc | |
巨能801 Magnum 801 | 0 | 35.35±7.50Aabc | 16.00±1.69Aa | 19.35±6.02Aabc | 0.86±0.19Bcde |
| 15 | 30.71±1.95Acd | 18.10±1.64Aab | 12.60±2.44Ac | 1.49±0.38Aa | |
| WL525 | 0 | 30.26±1.58Bc | 16.62±1.32Aa | 13.64±2.11Bcd | 1.24±0.26Ab |
| 15 | 37.67±3.54Ab | 18.81±5.10Aa | 18.85±3.65Abc | 1.05±0.43Aab | |
维多利亚 Victoria | 0 | 38.31±4.87Aab | 17.03±1.75Ba | 21.28±4.78Aab | 0.83±0.19Bde |
| 15 | 37.90±3.50Ab | 20.56±0.38Aa | 17.33±3.52Abc | 1.22±0.25Aab | |
迪特 Dieter | 0 | 40.71±4.99Aa | 17.47±1.78Aa | 23.23±4.37Aa | 0.77±0.17Be |
| 15 | 36.47±2.68Abc | 18.65±0.88Aa | 17.81±2.54Abc | 1.06±0.15Aab |
Table 3 Effects of drought stress on seedling growth of seven alfalfa cultivars
品种 Cultivars | PEG-6000浓度 PEG-6000 concentration (%) | 苗长 Seedling length (mm) | 芽长 Bud length (mm) | 根长 Root length (mm) | 芽根比 Bud root ratio |
|---|---|---|---|---|---|
皇冠 Phabulous | 0 | 28.74±3.20Bc | 18.37±2.07Aa | 10.37±1.63Bd | 1.79±0.28Aa |
| 15 | 46.33±4.12Aa | 15.04±1.22Bb | 31.28±3.30Aa | 0.48±0.04Bc | |
角斗士 Gladiator | 0 | 31.96±6.13Abc | 16.82±1.80Aa | 15.14±4.41Abcd | 1.15±0.23Abcd |
| 15 | 34.38±5.79Abc | 14.95±0.84Ab | 19.43±6.56Ab | 0.89±0.48Abc | |
| PANGO | 0 | 34.77±2.26Aabc | 18.70±1.24Aa | 16.07±2.74Abcd | 1.19±0.23Abc |
| 15 | 25.75±5.72Bd | 8.51±1.90Bc | 17.24±3.86Abc | 0.49±0.02Bc | |
巨能801 Magnum 801 | 0 | 35.35±7.50Aabc | 16.00±1.69Aa | 19.35±6.02Aabc | 0.86±0.19Bcde |
| 15 | 30.71±1.95Acd | 18.10±1.64Aab | 12.60±2.44Ac | 1.49±0.38Aa | |
| WL525 | 0 | 30.26±1.58Bc | 16.62±1.32Aa | 13.64±2.11Bcd | 1.24±0.26Ab |
| 15 | 37.67±3.54Ab | 18.81±5.10Aa | 18.85±3.65Abc | 1.05±0.43Aab | |
维多利亚 Victoria | 0 | 38.31±4.87Aab | 17.03±1.75Ba | 21.28±4.78Aab | 0.83±0.19Bde |
| 15 | 37.90±3.50Ab | 20.56±0.38Aa | 17.33±3.52Abc | 1.22±0.25Aab | |
迪特 Dieter | 0 | 40.71±4.99Aa | 17.47±1.78Aa | 23.23±4.37Aa | 0.77±0.17Be |
| 15 | 36.47±2.68Abc | 18.65±0.88Aa | 17.81±2.54Abc | 1.06±0.15Aab |
| 项目 Item | PC 1 | PC 2 | PC 3 | PC 4 | PC 5 |
|---|---|---|---|---|---|
| X1 发芽率Germination rate | 0.3652 | 0.1368 | 0.0529 | 0.0467 | 0.0586 |
| X2 发芽势Germination potential | 0.3210 | 0.0467 | 0.0848 | 0.3509 | 0.0437 |
| X3 发芽指数Germination index | 0.3546 | 0.1194 | 0.0780 | 0.1903 | 0.0571 |
| X4 活力指数Vitality index | 0.3623 | 0.0553 | 0.1358 | 0.0530 | 0.1770 |
| X5 干重Dry weight | 0.2628 | 0.3338 | 0.1266 | 0.1266 | 0.1628 |
| X6 鲜重Fresh weight | 0.1794 | 0.0350 | 0.3092 | 0.4302 | 0.3595 |
| X7 相对含水量Relative water content | 0.1712 | 0.4238 | 0.1137 | 0.1909 | 0.0863 |
| X8 苗长Seedling length | 0.0671 | 0.4335 | 0.2217 | 0.1878 | 0.0738 |
| X9 芽长Bud length | 0.2116 | 0.2089 | 0.3784 | 0.0678 | 0.2957 |
| X10 根长Root length | 0.2206 | 0.3782 | 0.0130 | 0.1789 | 0.1084 |
| X11 芽根比Bud root ratio | 0.3043 | 0.0915 | 0.2717 | 0.0553 | 0.3173 |
| X12 过氧化物酶活性Peroxidase activity | 0.2073 | 0.0589 | 0.0865 | 0.5371 | 0.1533 |
| X13 超氧化物歧化酶活性Superoxide dismutase activity | 0.1160 | 0.2880 | 0.3690 | 0.1704 | 0.3086 |
| X14 过氧化氢酶活性Catalase activity | 0.1521 | 0.3481 | 0.0467 | 0.3652 | 0.0905 |
| X15 丙二醛含量Malondialdehyde content | 0.0092 | 0.0310 | 0.5448 | 0.0762 | 0.2911 |
| X16 脯氨酸含量Proline content | 0.2596 | 0.0969 | 0.3492 | 0.1770 | 0.2753 |
| X17 可溶性糖含量Soluble sugar content | 0.1960 | 0.2620 | 0.1006 | 0.1874 | 0.5515 |
| 特征值 Eigenvalue | 6.806 | 3.797 | 2.953 | 1.861 | 1.123 |
| 贡献率Contribution rate (%) | 40.037 | 22.334 | 17.368 | 10.945 | 6.604 |
| 累积贡献率Cumulative contribution rate (%) | 40.037 | 62.371 | 79.740 | 90.685 | 97.289 |
| 权重 Weight | 0.412 | 0.230 | 0.179 | 0.113 | 0.068 |
Table 4 Principal component analysis of indicators of seven alfalfa cultivars under drought stress
| 项目 Item | PC 1 | PC 2 | PC 3 | PC 4 | PC 5 |
|---|---|---|---|---|---|
| X1 发芽率Germination rate | 0.3652 | 0.1368 | 0.0529 | 0.0467 | 0.0586 |
| X2 发芽势Germination potential | 0.3210 | 0.0467 | 0.0848 | 0.3509 | 0.0437 |
| X3 发芽指数Germination index | 0.3546 | 0.1194 | 0.0780 | 0.1903 | 0.0571 |
| X4 活力指数Vitality index | 0.3623 | 0.0553 | 0.1358 | 0.0530 | 0.1770 |
| X5 干重Dry weight | 0.2628 | 0.3338 | 0.1266 | 0.1266 | 0.1628 |
| X6 鲜重Fresh weight | 0.1794 | 0.0350 | 0.3092 | 0.4302 | 0.3595 |
| X7 相对含水量Relative water content | 0.1712 | 0.4238 | 0.1137 | 0.1909 | 0.0863 |
| X8 苗长Seedling length | 0.0671 | 0.4335 | 0.2217 | 0.1878 | 0.0738 |
| X9 芽长Bud length | 0.2116 | 0.2089 | 0.3784 | 0.0678 | 0.2957 |
| X10 根长Root length | 0.2206 | 0.3782 | 0.0130 | 0.1789 | 0.1084 |
| X11 芽根比Bud root ratio | 0.3043 | 0.0915 | 0.2717 | 0.0553 | 0.3173 |
| X12 过氧化物酶活性Peroxidase activity | 0.2073 | 0.0589 | 0.0865 | 0.5371 | 0.1533 |
| X13 超氧化物歧化酶活性Superoxide dismutase activity | 0.1160 | 0.2880 | 0.3690 | 0.1704 | 0.3086 |
| X14 过氧化氢酶活性Catalase activity | 0.1521 | 0.3481 | 0.0467 | 0.3652 | 0.0905 |
| X15 丙二醛含量Malondialdehyde content | 0.0092 | 0.0310 | 0.5448 | 0.0762 | 0.2911 |
| X16 脯氨酸含量Proline content | 0.2596 | 0.0969 | 0.3492 | 0.1770 | 0.2753 |
| X17 可溶性糖含量Soluble sugar content | 0.1960 | 0.2620 | 0.1006 | 0.1874 | 0.5515 |
| 特征值 Eigenvalue | 6.806 | 3.797 | 2.953 | 1.861 | 1.123 |
| 贡献率Contribution rate (%) | 40.037 | 22.334 | 17.368 | 10.945 | 6.604 |
| 累积贡献率Cumulative contribution rate (%) | 40.037 | 62.371 | 79.740 | 90.685 | 97.289 |
| 权重 Weight | 0.412 | 0.230 | 0.179 | 0.113 | 0.068 |
品种 Cultivars | 隶属函数值Subordinate function values μ(Xj ) | 综合评价值 Comprehensive evaluation (D) | 排序 Sort | ||||
|---|---|---|---|---|---|---|---|
| PC 1 | PC 2 | PC 3 | PC 4 | PC 5 | |||
| 皇冠Phabulous | 0.0000 | 0.1856 | 0.5822 | 0.4526 | 0.5167 | 0.2332 | 7 |
| 角斗士Gladiator | 0.2607 | 0.8494 | 1.0000 | 0.9071 | 0.8180 | 0.6399 | 2 |
| PANGO | 0.2695 | 0.6646 | 0.5640 | 0.0000 | 0.2457 | 0.3815 | 5 |
| 巨能801 Magnum 801 | 1.0000 | 1.0000 | 0.4875 | 0.4970 | 0.3074 | 0.8063 | 1 |
| WL525 | 0.9295 | 0.0000 | 0.8548 | 0.7233 | 0.1753 | 0.6296 | 3 |
| 维多利亚Victoria | 0.7198 | 0.3426 | 0.0000 | 0.5532 | 1.0000 | 0.5059 | 4 |
| 迪特Dieter | 0.1790 | 0.6234 | 0.0699 | 1.0000 | 0.0000 | 0.3426 | 6 |
Table 5 Subordinate function values and comprehensive evaluation values of seven alfalfa cultivars under drought stress
品种 Cultivars | 隶属函数值Subordinate function values μ(Xj ) | 综合评价值 Comprehensive evaluation (D) | 排序 Sort | ||||
|---|---|---|---|---|---|---|---|
| PC 1 | PC 2 | PC 3 | PC 4 | PC 5 | |||
| 皇冠Phabulous | 0.0000 | 0.1856 | 0.5822 | 0.4526 | 0.5167 | 0.2332 | 7 |
| 角斗士Gladiator | 0.2607 | 0.8494 | 1.0000 | 0.9071 | 0.8180 | 0.6399 | 2 |
| PANGO | 0.2695 | 0.6646 | 0.5640 | 0.0000 | 0.2457 | 0.3815 | 5 |
| 巨能801 Magnum 801 | 1.0000 | 1.0000 | 0.4875 | 0.4970 | 0.3074 | 0.8063 | 1 |
| WL525 | 0.9295 | 0.0000 | 0.8548 | 0.7233 | 0.1753 | 0.6296 | 3 |
| 维多利亚Victoria | 0.7198 | 0.3426 | 0.0000 | 0.5532 | 1.0000 | 0.5059 | 4 |
| 迪特Dieter | 0.1790 | 0.6234 | 0.0699 | 1.0000 | 0.0000 | 0.3426 | 6 |
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