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    20 February 2026, Volume 35 Issue 2
    Dynamic monitoring of land degradation in the Three-River Headwaters Region over the past 30 years using unoccupied aerial vehicle imagery and Landsat data
    Lin-xing ZHAO, Yan-he WANG, Zi-chao WANG, Ma-qiang XU, Ze-yu LI, Chang-xian QI, Bao-zu CUI, Zong-bao WANG
    2026, 35(2):  1-14.  DOI: 10.11686/cyxb2025108
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    Land degradation is a critical ecological and environmental issue that threatens ecosystem stability and food security in China. As a key ecological barrier in western China, the Three-River Headwaters Region is undergoing severe land degradation, which presents significant challenges for regional ecological security and socioeconomic development. Based on field sampling, unoccupied aerial vehicle (UAV) imagery, and Landsat data, this study established a multi-source data framework for monitoring land degradation by employing random forest (RF), support vector machine (SVM), and classification and regression tree (CART) models. The framework was applied to monitor land degradation dynamics in the Three-River Headwaters Region over the past three decades (1993-2003, 2003-2013, and 2013-2023), and to analyze its spatiotemporal evolution patterns. The results indicate that: 1) The integration of UAV and satellite data significantly improved the accuracy of degraded land detection. Among the models tested in this study, the RF model based on spectral and vegetation indexes and topographic features achieved the highest accuracy. Specifically, the accuracy of identifying desertified land reached 94.73% with an F1-score of 95.85%, while the accuracy of detecting degraded black soil beach land reached 90.98% with an F1-score of 95.18%. 2) From 1993 to 2023, the areas of non-degraded land and degraded black soil beach land initially increased and then decreased. The area of salinized land showed a fluctuating trend-increasing initially, then decreasing, and increasing again, while the area of desertified land continuously declined. For all degradation types, more than half of the affected areas remained in a stable state throughout the monitoring period. 3) Overall, both the severity of black soil beach degradation and desertification showed a decreasing trend, with substantial reductions in the area of severely degraded black soil land moderately desertified land. In contrast, there were only small changes in the area of mildly and moderately salinized land, but a notable decrease in the area of severely salinized land.

    Distribution of soil aggregates in different vegetation micro-patches in alpine meadows and the relationship with organic carbon mineralization
    Jing WANG, Xu-dong LI, Tian-hu HAN, De-cao NIU, Chun-li BAI, Ding GUO
    2026, 35(2):  15-27.  DOI: 10.11686/cyxb2025079
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    Micro-vegetation patchification is a critical process during the retrogressive succession of grasslands. This study explored how vegetation patches influence soil aggregate distribution and stability, and the subsequent effects on soil organic carbon (SOC) mineralization in the Qinghai-Tibet Plateau. Soil aggregates were fractionated using the wet-sieving method into three size classes: macro-aggregates (>250 μm), micro-aggregates (53-250 μm), and silt-clay fractions (<53 μm). SOC mineralization was quantified through indoor incubation experiments. It was found that macro-aggregates dominated all vegetation patches (55.31%-74.01%), but their content and aggregate stability significantly decreased in non-native patches compared to the native vegetation (CK). Notably, macro-aggregate loss was as high as 25.27% in bare areas (BA) (P<0.05). SOC content in macro-, micro-, and silt-clay fractions was highest in CK, Potentilla fruticosa, and Polygonum viviparum patches, followed by Ligularia virgaurea (LV) and BA. SOC cumulative mineralization quantity peaked in LV patches (12.38 g·kg?1) and was lowest in BA (8.26 g·kg?1). Additionally, cumulative mineralization rate was significantly higher in LV and BA than in other patches (P<0.05). Positive correlations were observed between SOC cumulative mineralization quantity and macro-aggregate content as well as aggregate stability (P<0.05). Conversely, cumulative mineralization rate was negatively correlated with aggregate stability and C/N ratio (P<0.01). These findings suggest that analyzing aggregate composition, stability, and mineralization dynamics during patchification processes can provide scientific insights for sustainable grassland management and accurate climate change assessments in the Qinghai-Tibet Plateau.

    Effects of short-term sheep manure return on soil quality of desert steppe
    Dong-ya LIU, Yan YANG, Jing LIU, Bo WANG, Zhi-gang LI
    2026, 35(2):  28-39.  DOI: 10.11686/cyxb2025084
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    The return of livestock manure through grazing plays a significant role in grassland soil nutrient cycling and improvement of physical properties. However, the impact of short-term livestock manure return on soil quality in desert steppes remains understudied. This research simulated sheep manure accumulation under different grazing intensities to explore the effects of four manure accumulation levels (MN, 0 kg·ha-1; ML, 4000 kg·ha-1; MM, 8000 kg·ha-1; MH, 16000 kg·ha-1) on soil quality of a desert steppe in Yanchi, Ningxia. The results demonstrated: 1) After two years of manure application, soil temperature tended to increase with increased manure accumulation level, although the average soil temperature differed significantly only in MH compared to MN, ML and MM (P<0.05). Similarly, average soil moisture content was significantly higher in MH than in ML and MM (P<0.05). 2) Soil pH decreased while extracellular enzyme activity increased with increased manure accumulation. 3) Manure return promoted the formation of soil macro-aggregates while reducing the proportion of micro-aggregates quantities, and the soil stability increased with increased accumulation of sheep manure. 4) Compared to MN, ML, MM and MH also significantly enhanced the soil quality index (P<0.05). Correlation analysis and structural equation modelling further revealed that manure return improved soil hydrothermal conditions, and stimulated enzyme activities related to carbon and nitrogen cycling, thereby increasing nutrient inputs and macro-aggregate formation, and ultimately enhancing soil quality. In conclusion, low, medium and high levels of manure application effectively improved soil quality, providing both a theoretical foundation and practical guidance for restoring degraded grassland soils.

    Species diversity and community characteristics of weeds on the banks of the Wenzhou expressway
    Jia-qi HE, Lin WU, Zhi-min QIU, Xu-yong GAO, Lun-lun GAO, Shuang-bin FU, Yan-ping YANG, Pei-long WANG, Wan XU, Zhuang ZHOU
    2026, 35(2):  40-53.  DOI: 10.11686/cyxb2025119
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    Weed invasion leads to the degradation of vegetation on the banks of roadways, affecting the recovery and stability of the ecosystem. Studying the species diversity and community characteristics of weeds on road banks is very important for understanding the pattern of weed community succession and for designing precise control methods. In this study, a contiguous grid quadrat method was used to investigate the vegetation on the road bank at seven sites along the Wenzhou Expressway in Zhejiang Province. We identified the species of weeds and the dominant species in the weed communities, and evaluated the diversity, stability, and similarity of weed communities. The results show that: 1) 65 species of weeds were present at the sampling sites, of which 47.69% were annual, 10.77% were annual or biennial, and 41.54% were perennial. Bidens albaGeranium carolinianumYoungia japonicaErigeron sumatrensisMiscanthus floridulusIpomoea triloba, and Lactuca indica were identified as the dominant weeds. 2) Patrick’s index and Shannon’s index were higher in B. alba-dominated communities than in other communities, whereas Simpson’s index and Pielou’s index were lower in B. alba-dominated communities than in other communities. Patrick’s index was lower in M. floridulus-dominated communities than in other communities, whereas Simpson’s index and Pielou’s index were higher. 3) The communities dominated by B. alba and M. floridulus were more stable than other communities. The similarity among different weed communities was low; i.e., the types and numbers of species differed among the communities. Overall, our results show that the weeds on the banks of the Wenzhou Expressway mainly belong to the Asteraceae and Poaceae families. Because of their different growth habits, the invasion of B. alba (the representative weed of the Asteraceae family) has increased the diversity of communities, while the invasion of M. floridulus (the representative weed of the Poaceae family) has reduced the diversity of communities. These results indicate that different types of plants should be considered according to the local situation when replanting road banks to promote the formation of a stable and sustainable community system and enhance resistance to weed invasion.

    A meta-analysis on the effect of fertilizer type on alfalfa seed yield under various climate and soil conditions
    Yang-kun LI, Zhuan-lin BEN, Jun-yu ZHANG, Hui-min YANG
    2026, 35(2):  54-67.  DOI: 10.11686/cyxb2025102
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    Supply of high-quality forage in sufficient quantity is a fundamental requirement for the development of efficient and sustainable animal production systems. To attain this goal, continuous expansion of the planting area of high-quality forages such as alfalfa is required. There is thus an urgent need to expand alfalfa seed production to meet the demand for alfalfa planting, but there remains a lack of information about effects of fertilization on alfalfa seed yield under different climatic and soil conditions. This study comprised a meta-analysis to investigate the effects of different fertilizer applications on alfalfa seed yield in China and explored the interactions between the fertilizer effect and climate and soil conditions. The results showed: 1) Fertilization could significantly increase alfalfa seed yield, with the greatest yield increase observed with the application of micronutrient fertilizers alone, while the application of potassium fertilizer alone and the combined application of nitrogen and potassium fertilizers led to yield reduction. 2) With increase in annual precipitation, the yield-enhancement effect of single fertilizer applications weakened, while the yield-enhancement effect of multi-fertilizer applications was strengthened. Higher annual temperatures resulted in weakening of the yield-enhancement effect, except in the case of micronutrient fertilizers alone. 3) The effect of soil factors on the fertilization effect varied significantly among different fertilization categories. Higher soil organic matter and total nitrogen content enhanced the yield-enhancement effect of most fertilization categories, while slightly weakening the yield-enhancement effect of micronutrient fertilizer application alone. Higher soil alkali-hydrolyzable nitrogen and available phosphorus content reduced the yield-enhancement effect of nitrogen and potassium application alone but increased the yield-enhancement effect of phosphorus application alone, with inconsistent effects on the yield-enhancement effects of multi-fertilizer applications. Higher soil available potassium content increased the yield-enhancement effect of all fertilization categories. In summary, this study provides guidance for optimizing fertilization management measures for alfalfa for seed production in different climatic regions and for different soils in China.

    Drought resistance evaluation of seed germination and seedling growth of seven alfalfa cultivars
    Ji-yuan ZHANG, Hai-quan AN, Jing-yi PAN, Chang LIU, Si-si LONG, Li-li ZHAO
    2026, 35(2):  68-82.  DOI: 10.11686/cyxb2025081
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    In this research a 15% polyethylene glycol-6000 (PEG-6000) solution was employed to simulate drought stress and test germination and seedling growth responses to drought of seven alfalfa (Medicago sativa) cultivars, namely, Phabulous, Gladiator, PANGO, Magnum 801, WL525, Victoria, and Dieter, for 14 days. The responses and changes in seed germination, seedling growth, and physiological and biochemical indexes to drought stress were measured and analyzed in the laboratory. It was found that under drought stress, the germination index and vigor index of the six alfalfa seeds, except PANGO, decreased significantly (P<0.05). The dry weight, seedling length and root length of Phabulous and WL525 seedlings increased significantly (P<0.05). The relative water content of seedlings of Magnum 801, Victoria and Gladiator increased significantly (P<0.05). The peroxidase (POD) activity of the six cultivars, except Gladiator, increased significantly (P<0.05), and the malondialdehyde (MDA) content increased significantly (P<0.05) in all cultivars except Victoria. The superoxide dismutase (SOD) activity increased significantly (P<0.05) in Phabulous, Dieter, and Magnum 801. The proline (Pro) and soluble sugar (SS) content increased significantly (P<0.05) in Gladiator. Using data for 17 traits defining seed germination, seedling growth and physiology and biochemistry, a multivariate evaluation was conducted using principal component analysis combined with the membership function method. In this way, the drought resistance of the seven alfalfa cultivars during seed germination and early seedling growth was ranked from strong to weak as follows: Magnum 801>Gladiator>WL525>Victoria>PANGO>Dieter>Phabulous. According to a hierarchical cluster analysis, the cultivars were classified into three categories: 1) Strongly drought-resistant cultivars are ‘Magnum 801’; 2) Cultivars with medium drought-resistance are ‘Gladiator’, ‘WL525’ and ‘Victoria’; 3) Varieties with weak drought-resistance are ‘PANGO’, ‘Dieter’ and ‘Phabulous’.

    Effects of herbage ground cover on orchard soil fertility enhancement in the Longdong Region of Gansu Province
    Hao-qi REN, Tong LI, Rong YANG, Hong WANG, Ming-xin ZHAO, Xue-zhou LIU, Shu-jun SONG, Kai SUN, Jun-wu XUE
    2026, 35(2):  83-94.  DOI: 10.11686/cyxb2025091
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    This study examined soil nutrient differences between clean-cultivated orchards and those with grass or similar ground cover to identify the effect of orchard management regime on soil properties in arid regions of China. We identified 45 pairs of orchards in 12 counties of the Longdong area of Gansu Province for sampling. The two orchards in each pair were near to each other and matched for age and type of fruit tree, but differed in that one was clean-cultivated, while the other was managed with herbage ground cover. Systematic analysis was conducted on the variations of soil organic matter (SOM), total nitrogen (TN), total carbon (TC), total phosphorus (TP), alkaline-hydrolyzable nitrogen (AN), and pH in the soil surface. The impacts of climatic conditions, soil types, and grass cultivation practices were deliberated upon. The results showed that: 1) In comparison to clean tillage, the soil SOM, TN, TC, AN, and pH levels were increased by 26.7%, 7.1%, 10.4%, 18.2%, and 2.5%, respectively, where herbage ground cover was present, while TP decreased by 8.3%. The 45 pairs of orchards were categorized for the same 6 parameters as increased, unchanged or decreased when herbage ground cover was present and the resulting frequency ratios of percentage of the 45 paired sites increased∶percentage of sites increased under herbage cover SOM, TN, TC, AN, pH and TP levels were 44.4%, 35.6%, 53.3%, 37.8%, 73.3% and 26.7%, and decreased were 24.4%, 33.3%, 24.4%, 26.7%, 22.2%, and 51.1%, respectively. 2) Annual rainfall showed a significant positive correlation with SOM, TC, AN, and pH (P<0.05). At sites with annual rainfall greater than 400 mm, both SOM and TC rates of change exceeded zero. However, annual air temperature did not have a significant impact on TP and pH, but exhibited a positive correlation with SOM and AN (P<0.05). 3) A significant positive correlation was observed between the number of years since establishment of herbage cover and the rates of change in TN, TP, AN, SOM, and TC (P<0.05). There was a trend of a negative linear association between pH and the number of years since herbage establishment, however, this trend did not achieve statistical significance. The rates of change in SOM, TN, TC, AN, and TP all increased with time since establishment of orchard ground cover, with TP showing the highest growth rate at 8.2 g·kg-1·yr-1. Furthermore, orchards with ground cover established for more than 4 years demonstrated higher levels of SOM and TN compared to those clean-cultivated orchards. 4) The rate of change of TP under sown vegetation cover (-18.3%) was significantly more negative than that in natural grassland (4.9%), while the opposite was true for pH, with sown ground cover exhibiting a faster rate of pH increase than naturally occurring herbage. The rate of TC increase in dark loessial soil was 25.7% higher than that in yellow loam soil, while the rate of TP decrease was 27.6% faster in dark loessial than yellow loam soil (P<0.05). These results offer a scientific foundation for optimizing and promoting orchard grassing techniques in the Longdong area. They can also assist fruit growers in selecting appropriate grassing methods and management practices based on local climate conditions and soil types, so as to improve orchard soil fertility and promote the sustainable development of orchard ecosystem.

    Creation of ethyl methanesulfonate chemical mutants of Hemarthria compressa seed stem
    Hui PENG, Lin MU, Jia-xin SHEN, Jing-xuan WANG, Jing HUANG, Yu-xun HUANG, Zhi-fei ZHANG
    2026, 35(2):  95-106.  DOI: 10.11686/cyxb2025092
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    Hemarthria compressa isa perennial species of the Poaceae family, with fast growth rate, strong adaptability and stress resistance. It is a very important forage resource in the southern region of China. This study investigated the use of ethyl methanesulfonate (EMS) as a chemical agent to produce mutants of H. compressa. Stems of seedlings were used to provide cells of H. compressa for exposure to EMS and two factors were tested: EMS concentration (0%, 0.2%, 0.4%, 0.6%, 0.8% or 1.0%) and treatment time (2, 4 or 6 h). It was found that the optimal EMS concentration and treatment time for mutagenesis of H. compressa is 0.6% and 6 hours. Phenotypic comparison testing identified an excellent H. compressa mutant among the EMS-mutatated population, designated number 5-5-4. Comparative testing of mutant 5-5-4 and the wild type under low phosphorus stress conditions showed that 5-5-4 had greater root and root tip number, longest root length and total root length, and a more developed root hair structure than the wild-type. Low phosphorus stress resulted in a significant decrease in phosphorus uptake, but the phosphorus use efficiency was greatly improved and the phosphorus utilization efficiency in roots and stems of mutant 5-5-4 was higher than in the wild type. Under low phosphorus stress, the activities of acid phosphatase, superoxide dismutase and peroxidase were also higher in roots of the mutant and roots of the mutant exhibited greater presence of phosphate-solubilizing bacteria, including BacillusRamlibacter and Rhodoferax in the rhizosphere. In summary, this study used EMS chemical mutagenesis technology to generate H. compressa mutants and analyzed the tolerance to low phosphorus stress and associated physiological parameters in a promising mutant, resulting in new germplasm resources with low phosphorus tolerance. These results provide both technical data for further mutagenesis work and breeding material development of improved H. compressa varieties to support animal husbandry in low phosphorus areas in southern China.

    Responses of leaf trait networks to extreme drought in typical steppe plants of Inner Mongolia
    Ya-nan QIAO, Hong-qiang WANG, Ying LI, Qiang YU
    2026, 35(2):  107-119.  DOI: 10.11686/cyxb2025118
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    With the intensification of global climate change, extreme drought events are occurring with increasing frequency, posing severe threats to plant survival and ecosystem functioning. Leaf trait networks, which integrate multiple functional traits and their interactions, provide a comprehensive framework for understanding plant responses to environmental stress. Although previous studies have examined the effects of extreme drought on individual or a few leaf traits, the response of the overall trait network structure and its key traits under extreme drought remains unclear. This study was conducted in the typical steppe of Inner Mongolia, where two types of extreme drought were simulated: a 66% reduction in precipitation from May to August each year (CHR), and a 100% reduction in precipitation during June and July each year (INT). We systematically measured 20 leaf traits and employed a trait network analysis approach to assess the impacts of extreme drought on trait variation and network properties. It was found that extreme drought significantly reduced leaf water potential and increased magnesium concentration, while weakening network connectivity and complexity, as indicated by decreases in the number of edges, edge density, and average clustering coefficient. Furthermore, by classifying the selected traits into hydraulic, compositional, and morphological categories, we found that hydraulic traits consistently exhibited the highest degree, closeness, and betweenness under both drought treatments. This highlights their central regulatory role in the network, suggesting that they play a dominant role in coordinating the responses of other functional traits to drought stress. This study provides novel insights into the integrated regulatory mechanisms of plant adaptation to extreme drought from a trait network perspective. Our findings enhance the understanding of plant drought resistance strategies and ecological adaptability, and offer a theoretical foundation for predicting plant ecological responses under future climate change scenarios.

    Comparison of skull characteristics between Rhombomys opimus and Meriones libycus in the southern desert of the Junggar Basin
    You-ping AN, Rong ZHANG, Jiu-qi ZHAO, Die CHEN, Su-wen YANG, Xiao-hui WANG, SUBINUER·jiapaer, Da-wei WANG
    2026, 35(2):  120-130.  DOI: 10.11686/cyxb2025082
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    To investigate the differences in skull morphological characteristics and their relationship with body weight between the sympatric desert rodents Rhombomys opimus and Meriones libycus in the southern margin of the Junggar Basin, and to explore whether they exhibit convergent adaptations to the hyperarid and high-temperature desert environment, this study conducted a rodent community survey using the snap-trapping method in the desert region of the southern Junggar Basin, Xinjiang Uygur Autonomous Region. Thirteen skull morphological indices, including total skull length, basal length, median palatal length, zygomatic width, and least breadth between the orbits, were measured from captured specimens. The interspecific differences in skull traits and their correlations with body weight were analyzed. Results indicated that: 1) Except for greatest length of the nasals (P=0.276), all 12 other skull indices exhibited highly significant interspecific differences (P<0.01); 2) Among the 13 skull indices, only the length of the down tooth row of M. libycus showed a significant intraspecific sexual dimorphism (P<0.05), while other indices displayed no significant sex-based differences (P>0.05); 3) Skull morphology trends relative to body weight revealed an extremely significantly positive correlation between profile length and body weight in R. opimusR2=0.4808, P<0.01). For M. libycus, basal length (R2=0.3214), zygomatic breadth (R2=0.6020), and lower jaw length (R2=0.5656) exhibited extremely significant positive correlations with body weight (P<0.01). In conclusion, R. opimus and M. libycus display distinct interspecific differences in multiple skull traits. Notably, profile length in R. opimus, and basal length, zygomatic breadth, and total length in M. libycus showed continued growth into adulthood, while other traits lacked clear patterns. These findings supplement taxonomic and morphological data for both species and provide a reference for species identification and evolutionary studies.

    Response of drying rate and nutrient content changes to the main environmental factors during alfalfa natural drying in irrigation areas
    Jian-min DU, Zhan-jun WANG, Wei WANG, Xue-peng MA, Dong-ning LI, Ji-xiang LI
    2026, 35(2):  131-142.  DOI: 10.11686/cyxb2025103
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    This study comprehensively analyzed the dynamics of drying rate and nutrient content during natural drying of alfalfa (Medicago sativa) in irrigated regions to better understand the key environmental factors influencing alfalfa hay drying. Meteorological and soil moisture data were collected and plant traits including water content, and major nutrient components (crude protein, CP; crude ash, Ash; ether extract, EE; neutral detergent fiber, NDF; acid detergent fiber, ADF; water-soluble carbohydrate, WSC; and relative feeding value, RFV) were measured in alfalfa with flattened stems harvested using mechanical equipment, at different cutting dates. The processes of water loss and nutrient changes were analyzed, and key environmental factors affecting drying rate and nutrient dynamics were identified through correlation analysis. Furthermore, the study divided the natural drying process of alfalfa into physiological (from cutting of alfalfa to when the whole-plant moisture content decreased to approximately 40%) and biochemical (the whole-plant moisture content decreased from 40% to 18%) drying stages, and analyzed and compared these two stages respectively. The results showed that: 1) The physiological drying stage accounted for 19.80%-52.25% of the total natural drying process, with the proportion decreasing gradually as cutting dates from May to September were delayed. During the physiological drying stage, drying rates followed the order: leaves>whole plant>stems; in contrast, the biochemical drying stage exhibited the order: stems>whole plant>leaves. Drying rates of stems, leaves, and whole plants in the physiological drying stage were 1.79-, 7.69-, and 3.43-fold higher, respectively, than those in the biochemical drying stage. 2) Contents of CP, WSC, EE, and RFV decreased throughout the drying process (including the beginning and end of drying, for both physiological and biochemical drying stages), whereas Ash, NDF, and ADF contents increased. Losses of EE, RFV, WSC or gains of Ash, NDF, ADF occurred during the physiological stage, with loss rates ranging from 52.09%-75.00% and gain rates from 48.53%-62.94%. CP loss primarily occurred during the biochemical drying stage, accounting for 71.18%-75.82% of total loss. 3) During alfalfa drying, environmental factors influenced drying rate in the order: air temperature>solar radiation intensity>wind speed>air relative humidity>0-10 cm soil moisture. The key factors (air temperature, solar radiation intensity, and wind speed) showed significant (P<0.05) or highly significant (P<0.01) positive correlations with drying rate. Nutrient content in alfalfa hay was primarily affected by air temperature, wind speed, air relative humidity, and 0-10 cm soil moisture.

    Analysis of weed species and community characteristics in soybean-maize fields with a banded compound planting pattern in Sichuan Province
    Shi-jie ZHOU, Yi-lian WANG, Pan SUN, Jing SHANG, Wen-yu YANG, Xue-gui WANG, Ji-zhi YANG
    2026, 35(2):  143-154.  DOI: 10.11686/cyxb2025110
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    In order to clarify the current situation of weeds in soybean (Glycine max)-maize (Zea mays) banded compound planting pattern fields in Sichuan Province, the occurrence of the weeds was investigated from 2023 to 2024 in 29 towns of 13 cities in Sichuan Province using an inverted W-shaped nine-point sampling method, and the relative abundance of weeds in each region was calculated to determine the dominant species. This was followed by analysis of community characteristics using the Berger-Parker index, Margalef index, Shannon-Wiener index, Simpson index, and Sorensen similarity coefficient to assess community structural similarities. soybean-maize In 2024, The highest dominance index of weeds was found in the southwestern Sichuan region, with a Berger-Parker index of 5.84, while the Simpson index, Shannon-Wiener index and Pielou index were lower than those for weeds in other regions, and where the dominant weeds were Digitaria sanguinalisAlternanthera philoxeroidesCommelina communis and Persicaria runcinata with relative dominance values of 25.92, 18.11, 13.48 and 10.00, respectively. The second-highest weed dominance was found in the eastern Sichuan region where Echinochloa crus-galliD. sanguinalis and A. philoxeroides were the dominant weeds, with relative dominance values of 35.65, 11.82 and 11.76, respectively. The Simpson index, Shannon Wiener index, and Pielou index in the western Sichuan region were higher than those in other regions, and the dominant weeds in this region were D. sanguinalisArthraxon hispidusE. crus-galli and A. philoxeroides, with relative dominance values of 33.46, 33.41, 7.61 and 6.68, respectively. The dominant weeds in the central Sichuan region were D. sanguinalisA. hispidusCalystegia hederacea and Cyperus rotundus, with relative dominance values of 36.16, 14.20, 7.95 and 7.11, respectively. The Sorensen similarity coefficient analysis revealed significant structural differences in weed communities among different regions, with similarity indices ranging between 0.5 and 0.6. From this analysis, D. sanguinalisA. hispidusA. philoxeroides and E. crus-galli emerge across different regions of Sichuan as the dominant weed species in soybean-maize fields sown with a banded compound planting pattern. The findings provide a theoretical basis for the scientific selection of targeted control agents and optimization of weed management strategies.

    Effects of stubble height and harvest period on the nutritional components and mycotoxin content of the silage maize cultivar ‘Yu Qingyu No. 3’ and in vitro rumen fermentation parameters for beef cattle
    Guo-cong LI, Fu-quan YIN, Ai-hong LAO, Yu-xin BAI, Sheng-nan WANG, Yan-li LU
    2026, 35(2):  155-166.  DOI: 10.11686/cyxb2025120
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    The aim of this experiment was to study the effects of stubble height and harvest time on the nutritional components and mycotoxin content of silage made from maize (Zea mays ‘Yu Qingyu No. 3’) as well as in vitro rumen fermentation parameters for beef cattle. The overall aim of this research is to provide a theoretical support for improving the feed value of silage maize. The silage maize cultivar ‘Yu Qingyu No. 3’ was cut to five different stubble heights (20, 30, 40, 50, and 60 cm) and harvested at four different stages (1/4, 1/2 and 3/4 milk line stage, and dough stage), a total of 20 treatments were tested, each replicated three times. According to the five-point sampling method, 10 silage maize plants in each stubble height treatment group were harvested at each harvest period. The harvested plants were crushed and then the fibers were cut to about 1 cm in length, and ensiled in a silage tank for 90 days. After ensiling, samples were analyzed for nutritional components, mycotoxin content, and in vitro rumen fermentation parameters for beef cattle. The results showed that: 1) The crude protein (CP), crude ash (Ash), acid detergent fiber (ADF), and neutral detergent fiber (NDF) contents and gross energy (GE) all differed significantly among the stubble height treatments and among the harvest period treatments (P<0.05). At a stubble height of 40 cm, the gross energy content showed a trend of initial increase and then decrease with the delay of the harvest time, reaching the maximum value at the 3/4 milk line stage. 2) The zearalenone (ZEN) and aflatoxin (AFs) contents in the maize differed significantly among the stubble height and harvest period treatments (P<0.05). At a stubble height of 50 cm, the zearalenone content showed a trend of initial decrease and then increase with the delay of the harvest date, reaching the minimum value at the 3/4 milk line stage. 3) In the in vitro fermentation analyses, the concentrations of acetic acid, propionic acid, and butyric acid at 24 and 48 hours differed significantly between the stubble height and the harvest time treatments (P<0.05). At the stubble height of 30 cm and harvest at the 3/4 milk line stage, the concentration of propionic acid reached the maximum value after 48 hours of in vitro fermentation, while the concentration of butyric acid was lowest at 24 hours and showed a pattern of initial decrease followed by an increase with delayed harvest. In summary, to produce high-quality silage, the maize cultivar ‘Yu Qingyu No. 3’ should be cut to a stubble height of 30-50 cm and harvested at the 3/4 milk line stage.

    The community structure and functional diversity of rhizosphere soil fungi of three plant species in the alpine grassland of Xinjiang
    Yi-xin SONG, Ming-yuan LI, Ya-sheng MAIRIYANGU, Ji-lian WANG
    2026, 35(2):  167-178.  DOI: 10.11686/cyxb2025089
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    This research investigated the rhizosphere fungal community structure and ecological functions among alpine grassland plant species (Taraxacum mongolicumPoa annua and Daucus carota) in the alpine grassland of Kizilsu Kirgiz Autonomous Prefecture, Xinjiang, using high-throughput sequencing technology. Correlations between soil physicochemical properties and fungal community structure were also analyzed. No significant differences in fungal alpha diversity were found among plant species. However, an analysis of similarities (ANOSIM) test indicated that the fungal community structure of P. annua was significantly different from that of the other two plant species. The dominant fungal phyla were identified as Ascomycota, Basidiomycota, and Mortierellomycota, while the predominant genera included ArchaeorhizomycesHygrocybeThelebolus, and Podospora. LefSe analysis identified PodosporaHygrocybe, and Archaeorhizomyces as biomarkers significantly associated with T. mongolicumP. annua, and D. carota, respectively. FUNGuild functional annotation revealed that nutritional modes of fungi across all samples were predominantly saprotrophic (27.1%-28.6%), with undefined saprotroph fungi being the most dominant functional group (18.5%-24.9%). Soil organic matter, alkali-hydrolyzable nitrogen, available phosphorus, and total nitrogen were identified as key determinants influencing fungal diversity. In conclusion, the structure and composition of rhizosphere fungal communities in the alpine grassland of Kizilsu Kirgiz Autonomous Prefecture was specific to plant species. These results provide a scientific basis for analyzing the mechanism of plant-microbe interactions and formulating sustainable management strategies for degraded grassland.

    Effects of common mycorrhizal networks on the growth and alkali tolerance of Pennisetum alopecuroides under different planting patterns
    Meng-hui YANG, Ya-jie LIU, Na LI, Chun-xue YANG
    2026, 35(2):  179-194.  DOI: 10.11686/cyxb2025145
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    Arbuscular mycorrhizal (AM) fungi connect the roots of different plants via common mycorrhizal networks (CMNs), mediating nutrient transfer and resource allocation among plants and influencing plant interactions. Soil salinization threatens the sustainability of grassland ecosystems, but the impact of CMNs on donor plant growth and alkali stress tolerance in alkaline environments remains unclear. In this study, Pennisetum alopecuroides inoculated with AM fungi served as the donor plant. Different transplanting patterns were established using compartmentalized pot experiments: no adjacent receiver plant on the other side of the root barrier mesh (neighborless plants), an adjacent uninoculated P. alopecuroides receiver plant (conspecific planting), and an adjacent uninoculated Plantago asiatica receiver plant (heterospecific planting). This experiment was designed to investigate the role of CMNs under alkali stress on the donor plant P. alopecuroides. The results demonstrate that: 1) Under alkali stress, the presence of neighboring plants (particularly conspecific neighbors) significantly increased the mycorrhizal colonization rate and colonization intensity in P. alopecuroides. 2) Under alkali stress and AM inoculation, P. alopecuroides in the conspecific neighbor treatment exhibited significantly higher mycorrhizal dependency and soil easily extractable glomalin-related soil protein content compared with both the neighborless and heterospecific neighbor treatments. 3) Alkali stress significantly inhibited the growth of P. alopecuroides, but the establishment of CMNs alleviated this effect. 4) Under alkali stress, CMNs significantly enhanced the photosynthetic capacity, levels of osmotic adjustment substances, and antioxidant enzyme activities in P. alopecuroides, while reducing malondialdehyde and superoxide anion radical contents. 5) A subordinate function analysis indicated that under alkali stress and AM inoculation, the conspecific neighbor treatment conferred the strongest alkali tolerance upon P. alopecuroides. The results of this study demonstrate that AM fungus-driven CMNs can promote the growth of P. alopecuroides and enhance its alkali tolerance by mediating plant-plant interactions. Notably, CMNs formed with conspecific neighbors had the most pronounced beneficial effect on P. alopecuroides.

    Bacterial community assembly in the rhizosphere and endosphere of different perennial alfalfa varieties with low fall dormancy rates
    Zhao-ming WANG, Li-na ZHENG, Yue-hua ZHANG, Wei ZHAO, Xiang CHEN, Zhen-yu JIA
    2026, 35(2):  195-207.  DOI: 10.11686/cyxb2025190
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    Plant growth and stress resilience rely on beneficial interactions between roots and symbiotic microorganisms. Low fall dormancy alfalfa (Medicago sativa) varieties exhibit strong adaptability to short-day and low-temperature conditions, but the characteristics of their root-associated microbial assemblies and cultivar-specific variations remain unclear. In this study, four low fall dormancy alfalfa cultivars-‘Lanmu No. 1’, ‘Gongnong No. 1’, ‘Golden empress’ (all M. sativa), and ‘Gannong No. 1’ (Medicago lupulina) -were cultivated at a field experimental station in Gannan, Gansu Province. Using 16S rRNA gene amplicon sequencing, we analyzed rhizospheric and endophytic bacterial communities and their assembly mechanisms. The results show that bacterial alpha diversity was significantly higher in the rhizosphere than in endosphere, with distinct community structures observed among cultivars. Compared with the other cultivars, the cold-tolerant cultivar ‘Lanmu No. 1’ was uniquely enriched with Streptacidiphilus in the rhizosphere, showed increased endophytic bacterial diversity, and greater complexity and connectivity of microbial co-occurrence networks. Analysis of community assembly processes revealed that both rhizospheric and root endophytic communities were primarily governed by heterogeneous selection (42%-55%) and homogeneous selection (56%-83%). Notably, deterministic selection had a stronger influence on root-associated communities in ‘Lanmu No. 1’, ‘Gongnong No. 1’, and ‘Gannong No. 1’ than in ‘Golden empress’. This study elucidates the assembly patterns and cultivar-driven divergence of root-associated microbiomes in low fall dormancy alfalfa. These results provide a theoretical foundation for microbiome manipulation to improve forage crop adaptability in cold, high-altitude regions.

    Effects of γ-aminobutyric acid on cadmium stress tolerance and cadmium uptake and transport in creeping bentgrass
    Yi-lin DI, Si-tian LIU, Xin-ying LIU, Yong DU, Zhou LI
    2026, 35(2):  208-220.  DOI: 10.11686/cyxb2025112
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    Cadmium (Cd) is a common heavy metal pollutant in soil that seriously reduces the growth and development of turfgrasses. γ-Aminobutyric acid (GABA) is an important growth regulator in plants, and is involved in regulating tolerance to abiotic stress. In this study, we determined the effects of exogenous GABA on the endogenous GABA content, chlorophyll fluorescence parameters, cell membrane stability, and Cd uptake and transport in different organs of creeping bentgrass (Agrostis stolonifera). The results show that Cd stress significantly reduced the relative water content, chlorophyll content, and photochemical efficiency in leaves, leading to membrane lipid peroxidation and reduced cell membrane stability. The application of 0.5 mmol·L-1 GABA significantly increased the endogenous GABA content, chlorophyll content, relative water content, photochemical efficiency (Fv/Fm), and performance index on an absorption basis (PIABS), and reduced the accumulation of malondialdehyde and electrolyte leakage in leaves of creeping bentgrass under Cd stress. The Cd content in leaves and roots increased sharply under Cd stress, but Cd accumulated to much higher levels in the roots than in the leaves. Exogenous application of GABA significantly reduced Cd accumulation in the leaves, but increased Cd accumulation in the roots, indicating that GABA inhibited the transport of Cd from roots to aboveground parts. This process may be related to changes in gene expression, because application of GABA resulted in significant upregulation of AsZIP2AsNRAMP1, and AsNRAMP5 in roots, and downregulation of AsNRAMP1 and AsNRAMP5 in leaves. In addition, GABA significantly upregulated AsHMA1AsHMA3AsABCC2, and AsABCC4 in the roots of plants under Cd stress, which promoted the compartmentalization of Cd ions into vacuoles to reduce Cd toxicity. These results not only provide new information about the mechanism by which GABA regulates Cd tolerance in plants, but also provide a technical reference for the cultivation and management of cold-season turfgrass in Cd-polluted soils.

    A comparative study of differences in condensed tannin synthesis between Medicago sativa and Onobrychis viciifolia at different growth stages
    Li-juan CHEN, Rong GAO, Jian-xi WANG, Hui-ling MA
    2026, 35(2):  221-236.  DOI: 10.11686/cyxb2025115
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    In this study, we explored the differences in condensed tannin (CT) synthesis and accumulation between alfalfa (Medicago sativa) and sainfoin (Onobrychis viciifolia). Stems and leaves of the alfalfa cultivars ‘Gannong No. 3’, ‘Gannong No. 7’, and ‘Elite’, and the sainfoin ‘Gansu’ were sampled across six growth stages from seedling to maturity. The contents of total phenolics, total flavonoids, CTs, and secondary metabolites (rutin, gallic acid, epicatechin gallate, epicatechin, catechin) were determined. In addition, to decipher the regulatory mechanism of condensed tannin synthesis, we measured the activities of key enzymes in the phenylpropanoid metabolic pathway (phenylalanine ammonia-lyase, PAL; 4-coumarate-CoA ligase, 4CL; cinnamate 4-hydroxylase, C4H) and the flavonoid pathway (anthocyanidin reductase, ANR and leucoanthocyanidin reductase, LAR). The results show that: 1) The condensed tannin content varied significantly among growth stages, peaking at the bud stage in O. viciifolia ‘Gansu’ and the pod-setting stage in M. sativa, while the condensed tannin content was generally higher in leaves than in stems. 2) The trends in the contents of total flavonoids and rutin were analogous to those of condensed tannins. 3) The activities of 4CL, ANR, PAL, LAR, and C4H significantly increased at the bud stage of O. viciifolia ‘Gansu’ and the pod-setting stage of M. sativa, indicative of enhanced metabolic flow of the phenylpropanoid pathway towards condensed tannin synthesis. Among the alfalfa varieties, M. sativa ‘Gannong No. 3’ exhibited significantly higher condensed tannin content at the pod-setting stage (P<0.05), suggesting stronger tannin synthesis potential. The results of this study clarify the dynamic correlations between key CT synthesis enzymes and metabolites, providing a theoretical basis for metabolic regulation of CT in forage crops and for breeding high-tannin varieties.