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草业学报 ›› 2014, Vol. 23 ›› Issue (2): 253-259.DOI: 10.11686/cyxb20140230

• 研究论文 • 上一篇    下一篇

高效稳定纤维素分解菌群筛选及其分解特性研究

王得武1,姚拓2,杨巧丽1,韩华雯2,张英2,卢虎2,滚双宝1*   

  1. 1.甘肃农业大学动物科学技术学院,甘肃 兰州 730070;
    2.甘肃农业大学草业学院,甘肃 兰州 730070
  • 收稿日期:2013-03-13 出版日期:2014-02-25 发布日期:2014-04-20
  • 通讯作者: E-mail:gunsb@gsau.edu.cn
  • 作者简介:王得武(1987-),男,甘肃平川人,在读硕士。E-mail:wangdw66@163.com
  • 基金资助:
    十二五“国家科技支撑计划项目课题”西北绿洲农牧循环技术集成与示范(No. 2012BAD14B10-5),甘肃省农业科技创新项目(No. GNCX-2012-45)和甘肃省农业科技创新项目“新型生态养猪关键技术开发、集成与示范”(GNCX-2009-13)资助。

Screening and degradation characterization of efficient and stable cellulose degrading microbial communities

WANG De-wu1, YAO Tuo2, YANG Qiao-li1, HAN Hua-wen2, ZHANG Ying2, LU Hu2, GUN Shuang-bao1   

  1. 1.College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2.Pratacultural College, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2013-03-13 Online:2014-02-25 Published:2014-04-20

摘要: 为获得能够在常温条件下(28~32℃)快速分解纤维素的微生物群体,利用限制性培养基从不同材料(森林腐殖质、腐烂的玉米秸秆、牛场料槽旁土样、麦垛底部土样和牛鸡粪混合储粪池中土样)的土样中筛选纤维素分解菌群,采用失重法测定菌群对不同纤维材料的分解能力及其在不同初始酸碱条件下的分解特性,用固体平板法对其菌株组成特性进行了初步探究。结果表明,不同材料筛选的纤维素分解菌群中,以牛鸡粪混合储粪池土样筛选的菌群分解效果最佳,该菌群48 h能将培养基内滤纸分解成糊状;6 d对滤纸、玉米秸秆、稻草秸秆、小麦秸秆、柞木木屑和杨木木屑分解率分别为94.95%,48.52%,45.05%,44.30%,11.00%,1.22%。在发酵液初始 pH 5~11范围内,对滤纸分解率超过84.59%,并将反应体系pH最终调节稳定在8.5~8.8;该菌群经分离纯化得到的8株真菌、6株细菌和3株放线菌相互接种构建的人工菌群不具备纤维素分解能力。筛选的纤维素分解菌群能够高效分解纤维素,同时对秸秆类木质纤维材料也具有较强的分解能力。

Abstract: A range of materials (primeval forest humus, rotten corn straw, soil samples near by cattle farm trough, bottom of wheat-rick, cattle and chicken feces compost) were screened on restrictive media at room temperature to obtain efficient, cellulose degrading, microbial communities. The capability for degradation and decomposition of fibrous materials at different initial pH conditions were analyzed using a weight loss method. The composition and characterization of this microbial system was identified using a solid plate culture method. The cellulose microbial communities screened from cattle and chicken faeces compost were better than those from other materials for decomposition of cellulose. They could decompose filter paper into a paste after 48 hours culture, and filter paper, corn straw, rice straw, wheat straw, xylosma and poplar wood chips were degraded by 94.95%, 48.52%, 45.05%, 44.30%, 11.00% and 1.22% respectively after 6 days continues cultivation. With an initial pH of 4 to 10 of the zymotic fluid reaction system, cellulose microbial communities could degraded 84.59% of the filter paper, and the pH finally stabilized in the range of 8.5-8.8. Eight fungal strains, 6 bacterial strains and 3 actinomycete strains were isolated and purified from this cellulose microbial community. these strains were incubated into each other artificially, but could not degrade cellulose. The results of this study indicate that cellulose microbial communities had strong capability to degrade cellulose and straw class lignocellulose.

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