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草业学报 ›› 2018, Vol. 27 ›› Issue (5): 201-209.DOI: 10.11686/cyxb2017247

• 研究论文 • 上一篇    下一篇

尼龙袋法评定苜蓿和谷草的组合效应评估

庄二林, 王慧娟, 田秀娥, 万美娇, 张阳, 王永军*   

  1. 西北农林科技大学动物科技学院,陕西 杨凌 712100
  • 收稿日期:2017-05-22 修回日期:2017-07-27 出版日期:2018-05-20 发布日期:2018-05-20
  • 通讯作者: * E-mail: dkxywyj@163.com
  • 作者简介:庄二林(1989-),男,河南新乡人,硕士。E-mail:1090034246@qq.com
  • 基金资助:
    科技部公益性行业专项“北方作物秸秆饲用化利用技术研究与示范”(201503134)资助

Evaluating the associate effect of alfalfa hay and millet stalks with Nylon-bag technique

ZHUANG Er-lin, WANG Hui-juan, TIAN Xiu-e, WAN Mei-jiao, ZHANG Yang, WANG Yong-jun*   

  1. College of Animal Science and Technology, Northwest Agriculture and Forestry University, Yangling 712100, China
  • Received:2017-05-22 Revised:2017-07-27 Online:2018-05-20 Published:2018-05-20
  • Contact: * E-mail: dkxywyj@163.com

摘要: 为研究苜蓿和谷草以何种比例搭配组合可以较好地发挥它们之间的组合效应,以5只安装永久瘤胃瘘管的小尾寒羊作为试验动物,采用5×5拉丁方试验设计,将谷草和苜蓿分别以不同比例(0∶100、100∶0、25∶75、50∶50、75∶25)组成粗饲料,与精料补充料以精粗比40∶60组成5种日粮(Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ),通过尼龙袋法测定日粮主要营养成分的瘤胃降解率。以粗蛋白(CP)和干物质(DM)瘤胃有效降解率EDCP和EDDM作为计算组合效应指数的主要观察测定指标,计算组合效应指数;同时将中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)瘤胃有效降解率EDNDF和EDADF作为对EDDM进行剖分的二级观察指标,揭示组合效应的发生机理。结果表明:1)各组EDDM、EDCP、EDNDF和EDADF均随组合中苜蓿比例的增加而显著提高(P<0.05);2)以EDDM为指标评定各日粮的组合效应时,Ⅲ、Ⅳ和Ⅴ三组的组合效应指数分别为0.8%、4.0%和3.7%;以EDCP为指标评定各日粮的组合效应时,Ⅲ、Ⅳ、Ⅴ三组的组合效应指数分别为2.5%、3.5%和3.1%,Ⅳ组基于EDDM和EDCP计算的组合效应指数均大于Ⅲ和Ⅴ组,效果最好;3)Ⅲ、Ⅳ、Ⅴ三种日粮中CF(粗纤维)对DM组合效应指数的贡献率分别为101.55%、68.75%和55.41%。

关键词: 苜蓿, 谷草, 尼龙袋法, 有效降解率, 组合效应

Abstract: This study aimed to investigate the optimal proportion of millet stalks in a mixed diet of millet stalks and alfalfa hay. Five small-tailed Han sheep with permanent rumen fistulae were selected as experimental animals and a 5×5 Latin square design was set up with five individual sheep as replicates to test each of five dietary mixtures. All five diets were composed of a 40∶60 mixture of concentrate and roughage. However, the roughage varied in the proportion of millet stalks and alfalfa hay (0∶100, 100∶0, 25∶75, 50∶50, 75∶25, for diets Ⅰ, Ⅱ, Ⅲ, Ⅳ, and Ⅴ, respectively). The digestibility and effective degradability (ED) of major nutrition composition in diets were measured using Nylon-bag technique. EDDM and EDCP were used as the primary assessment index to calculate an associate effects index (AEI); EDNDF and EDADF were used as a secondary index to reveal the mechanism of the associative effect between millet stalks and alfalfa hay. It was found that: 1) The ED of DM, CP, NDF and ADF was significantly increased with increasing proportion of alfalfa hay (P<0.05). 2) The AEI of groups Ⅲ, Ⅳ, and Ⅴ was 0.8%, 4.0% and 3.7%, respectively, when the EDDM was the only evaluation index, while the AEI of groups Ⅲ, Ⅳ, and Ⅴ, was 2.5%, 3.5% and 3.1%, respectively, when the EDCP was the only evaluation index. Thus, diet Ⅳ was the best. 3) In diets Ⅲ, Ⅳ, and Ⅴ the respective contributions of CF to the AEIDM were 101.55%, 68.75% and 55.41%.

Key words: alfalfa hay, millet stalks, Nylon-bag technique, effective degradability, associate effects