铝胁迫对西藏野生垂穗披碱草种子萌发及幼苗生长的影响

doi:10.11686/cyxb2017341

草业学报 ›› 2018, Vol. 27 ›› Issue (7): 155-165.DOI: 10.11686/cyxb2017341

铝胁迫对西藏野生垂穗披碱草种子萌发及幼苗生长的影响

蔺永和^1,2, 吴景², 方江平¹, 张卫红³, 苗彦军^1,*, 李勇胜²

1.西藏农牧学院,西藏林芝 860000;
2.兰州大学草地农业科技学院,甘肃兰州 730020;
3.扬州大学动物科学与技术学院,江苏扬州225009

收稿日期:2017-08-11 修回日期:2017-10-26 出版日期:2018-07-20 发布日期:2018-07-20
通讯作者: * E-mail:myj666@126.com
作者简介:蔺永和(1992-),男,甘肃定西人,在读硕士。E-mail:1278171543@qq.com
基金资助:
国家重点研发计划项目(2016YFC0502002;2016YFC0502006),西藏自治区科技厅重点项目(2017ZDKJZC-15;2016KJTZD01)和西藏农牧学院“雪域英才工程”支持项目(2015XYB03)资助

Effects of aluminum stress on germination characteristics and seedling growth of wild Elymus nutans seeds in Tibet

LIN Yong-he^1,2, WU Jing², FANG Jiang-ping¹, ZHANG Wei-hong³, MIAO Yan-jun^1,*, LI Yong-sheng²

1.Tibet Agricultural and Animal Husbandry College, Nyingchi 860000, China;
2.College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
3.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2017-08-11 Revised:2017-10-26 Online:2018-07-20 Published:2018-07-20
Contact: * E-mail:myj666@126.com

摘要/Abstract

摘要： 采用水培的方法,以采自西藏高寒区域不同地区的野生垂穗披碱草种子为材料,初步探讨了酸性环境下(pH 4.5)不同浓度的Al³⁺(0、0.5、1.0和1.5 mmol·L^-1)胁迫对垂穗披碱草种子萌发及幼苗生长的影响,以期为酸铝土壤的改良提供优良的种质资源。研究结果显示,低浓度Al³⁺(0.5、1.0 mmol·L^-1)对3种垂穗披碱草种子的发芽率无显著影响(P>0.05),但Al³⁺浓度为1.5 mmol·L^-1时,3种垂穗披碱草种子的发芽率均被显著抑制(P<0.05)。同时,3种野生牧草的根长、株高、根重及茎叶重在Al³⁺胁迫下受到了不同程度的抑制,且根系被抑制的程度大于茎叶。此外,植物幼苗中丙二醛和可溶性糖作为受胁迫程度的重要指标,其含量在3种垂穗披碱草幼苗中均随Al³⁺浓度的升高呈现出上升的趋势。结果还表明,1.5 mmol·L^-1 Al³⁺处理时,那曲、申扎、浪卡子垂穗披碱草根系中铝含量分别是地上部的12.2、15.3、17.5倍。铝毒和磷缺失往往是酸性土壤中同时存在的问题,这严重影响了植物生长,研究表明Al³⁺抑制了垂穗披碱草对磷的吸收及转运。通过隶属函数法对3种垂穗披碱草的抗铝特性评估结果显示,采自申扎县的垂穗披碱草对Al³⁺的耐性较高,可驯化育种,为酸性土壤的改良提供优质的种质资源。

关键词: 垂穗披碱草, 铝胁迫, 种子萌发, 幼苗生长, 铝富集, 磷含量, 西藏

Abstract: In general, Elymus nutans grows well in hostile environments at high altitudes. Aluminum (Al) stress is one of the serious factors limiting plant productivity in acidic soils. However, studies of the influence of aluminum on this plant are still lacking. The seeds of wild E. nutans from three different areas in Tibet (Naqu, Shenza and Langkazi Counties) were used as materials in this study. The major experimental method involved hydroponic culture with four concentrations of Al³⁺ (0, 0.5, 1.0 and 1.5 mmol·L^-1) under acidic conditions (pH 4.5). In order to provide robust germplasm resources for the improvement of acid-aluminum soil, the effects of Al³⁺ stress on the seed germination and seedling growth were initially explored. Our investigations indicated that there were no significant differences (P>0.05) in the germination rate of the three samples of wild E. nutans seeds at lower concentrations of Al³⁺ stress (0.5 and 1.0 mmol·L^-1) compared to the control, but that results were adverse at higher concentrations (1.5 mmol·L^-1). The results showed that root length, plant height, root weight and shoots’ weight in the three samples decreased with increasing Al³⁺ concentration. In addition, the MDA and soluble sugar contents of seedlings substantially increased with Al³⁺ application. Data showed that Al was poorly translocated from the roots to aerial parts of the three seedling samples. In the treatment groups, the Al content of roots increased 12.2, 15.3 and 17.5 times more than that of the aerial parts. Al toxicity and phosphorus (P) deficiency often co-exist in acidic soils and limit plant growth and crop production. In this study, we observed that Al stress induced a reduction in P accumulation in the plants. Using eight indicators for seedlings under Al stress, we performed a comprehensive evaluation of Al tolerance of the three E. nutans samples and showed that the Shenza County sample was highly tolerant to aluminum and so could be used for domestication and breeding to provide good germplasm resources for the improvement of acid-aluminum soils.

Key words: Elymus nutans, aluminum stress, seeds germination, seedlings growth, aluminum accumulation, phosphorus content, Tibet

蔺永和, 吴景, 方江平, 张卫红, 苗彦军, 李勇胜. 铝胁迫对西藏野生垂穗披碱草种子萌发及幼苗生长的影响[J]. 草业学报, 2018, 27(7): 155-165.

LIN Yong-he, WU Jing, FANG Jiang-ping, ZHANG Wei-hong, MIAO Yan-jun, LI Yong-sheng. Effects of aluminum stress on germination characteristics and seedling growth of wild Elymus nutans seeds in Tibet[J]. Acta Prataculturae Sinica, 2018, 27(7): 155-165.

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铝胁迫对西藏野生垂穗披碱草种子萌发及幼苗生长的影响

Effects of aluminum stress on germination characteristics and seedling growth of wild Elymus nutans seeds in Tibet

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