环境因子对中国柽柳遗传变异的影响

doi:10.11686/cyxb2018691

草业学报 ›› 2019, Vol. 28 ›› Issue (10): 178-186.DOI: 10.11686/cyxb2018691

环境因子对中国柽柳遗传变异的影响

孙丽坤^1,*, 刘光琇², 张宝贵³, 章高森²

1.甘肃农业大学,甘肃兰州 730070;
2.中国科学院西北生态环境资源研究所,甘肃兰州 730000;
3.太原师范学院,山西晋中 030619

收稿日期:2018-10-15 修回日期:2018-11-28 出版日期:2019-10-20 发布日期:2019-10-20
通讯作者: E-mail: sunlk_baby@126.com
作者简介:孙丽坤(1987-),女,山东泰安人,讲师,博士。E-mail: sunlk_baby@126.com
基金资助:
甘肃省青年科技基金计划(18JR3RA181)和甘肃农业大学学校人才专项经费(2017RCZX-19)资助

Effects of environmental factors on population genetic diversity of Tamarix chinensis

SUN Li-kun^1,*, LIU Guang-xiu², ZHANG Bao-gui³, ZHANG Gao-sen²

1.Gansu Agricultural University, Lanzhou 730070, China;
2.Northwest Institute of Eco-Environmental and Resources, Chinese Academy of Sciences, Lanzhou 730000, China;
3.Taiyuan Normal University, Jinzhong 030619, China

Received:2018-10-15 Revised:2018-11-28 Online:2019-10-20 Published:2019-10-20
Contact: E-mail: sunlk_baby@126.com

摘要/Abstract

摘要： 分别以核糖体DNA(nuclear ribosomal DNA, nrDNA)的内转录间隔区(internal transcribed spacer regions, ITS)和两条叶绿体DNA(chloroplast DNA, cpDNA)片段—trnL-trnF, rps16为分子标记,研究了20个自然分布的中国柽柳群体遗传变异与环境因子和地理距离间的相关性。结果表明:ITS序列(616 bp)中共发现了10个多态位点,定义了11种单倍型;总核苷酸多样性和总单倍型多样性分别为2.170和0.814。两个cpDNA分子标记片段的拼接序列(1542 bp)中共发现了14个多态位点,定义了16种单倍型,总核苷酸多样性和总单倍型多样性分别为0.500和0.586。ITS遗传多样性与地理、气候和土壤因子间相关性分析显示:海拔、温度和经度是影响中国柽柳群体遗传变异的主要因素。在低海拔、温暖和靠海近的湿润东部群体,ITS遗传多样性较高。而cpDNA的遗传变异与各种环境因子间没有显著的相关性。Mantel检测发现中国柽柳ITS的遗传变异与地理距离显著正相关,而cpDNA的遗传变异没有显著的地理渐变趋势,该结果进一步揭示了中国柽柳强大的种子流在降低群体间遗传分化上发挥了重要作用。

关键词: 中国柽柳, 核苷酸多样性, 单倍型多样性, 遗传分化, 环境因子

Abstract: In this study, we examined the relationships between environmental factors and the genetic diversity of 20 Tamarix chinensis. populations throughout its native range. Genetic diversity was evaluated by comparing the sequences of nuclear ribosomal internal transcribed spacer regions (ITS) and two chloroplast DNA (cpDNA) regions (trnL-trnF and rps16). Eleven haplotypes were identified on the basis of 10 variable sites within the 616 bp sequenced ITS region, and the total nucleotide diversity and haplotype diversity indexes were 2.170 and 0.814, respectively. Sixteen haplotypes were identified on the basis of 14 variable sites within the two chloroplast regions (1542 bp), and the total nucleotide diversity and haplotype diversity indexes were 0.500 and 0.586, respectively. The results showed that the genetic diversity based on the ITS region is influenced mainly by altitude, temperature, and longitude. Populations with rich genetic diversity exist at lower altitudes, in warmer habitats, and in eastern regions nearer to the sea. We detected no significant relationships between the genetic diversity of cpDNA and environmental variables. Using the Mantel test, a significant relationship was detected between matrices of ITS genetic differentiation and matrices of geographical distance, but this relationship was not detected from the cpDNA data. This result further proved that seed flow is an important factor contributing to the low genetic differentiation of T. chinensis populations.

Key words: Tamarix chinensis, nucleotide diversity, haplotype diversity, genetic differentiation, environmental factors

孙丽坤, 刘光琇, 张宝贵, 章高森. 环境因子对中国柽柳遗传变异的影响[J]. 草业学报, 2019, 28(10): 178-186.

SUN Li-kun, LIU Guang-xiu, ZHANG Bao-gui, ZHANG Gao-sen. Effects of environmental factors on population genetic diversity of Tamarix chinensis[J]. Acta Prataculturae Sinica, 2019, 28(10): 178-186.

参考文献

[1] Scheiner S M.Genetics and evolution of phenotypic plasticity. Annual Review of Ecology & Systematics, 1993, 24(1): 35-68.
[2] Frankham R.Challenges and opportunities of genetic approaches to biological conservation. Biological Conservation, 2010, 143(9): 1919-1927.
[3] Hao L J, Lin S Z, Xie L, et al. Analysis of genetic diversity of Pisticia chinensis (Anacardiaceae) by microsatellite markers. Genomics and Applied Biology, 2011, 30(10): 1055-1064.
郝丽娟, 林善枝, 谢磊, 等. 黄连木遗传多样性的SSR分析. 基因组学与应用生物学, 2011, 30(10): 1055-1064.
[4] Parmesan C.Ecological and evolutionary responses to recent climate change. Annual Review of Ecology Evolution & Systematics, 2006, 37(1): 637-669.
[5] Klimeš L, DolezžAl J. An experimental assessment of the upper elevational limit of flowering plants in the western Himalayas. Ecography, 2010, 33(3): 590-596.
[6] Xu Y L, Cai N H, Chen S, et al. Relationships between the genetic diversity of Pinus yunnanensis Franch. natural populations and ecological factors. Chinese Journal of Ecology, 2016, 35(7): 1767-1775.
许玉兰, 蔡年辉, 陈诗, 等. 云南松天然群体遗传变异与生态因子的相关性. 生态学杂志, 2016, 35(7): 1767-1775.
[7] Shah J M, Asgher Z, Zeng J B, et al. Growth and physiological characterization of low nitrogen responses in Tibetan wild barley (Hordeum spontaneum) and cultivated barley (Hordeum vulgare). Journal of Plant Nutrition, 2017, 40(6): 861-868.
[8] Ma D W, Wang S H, Luo T, et al. Effects of environmental factors on the genetic diversity of Pogonatherum paniceum. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2006, 45(2): 73-77.
马丹炜, 王胜华, 罗通, 等. 环境因子对岩生植物金发草遗传多样性的影响. 中山大学学报: 自然科学版, 2006, 45(2): 73-77.
[9] Xu B, Sun G L, Wang X M, et al. Population genetic structure is shaped by historical, geographic, and environmental factors in the Leguminous shrub Caragana microphylla, on the Inner Mongolia Plateau of China. BMC Plant Biology, 2017, 17(200):1-12.
[10] Mjf B, Jaf D F, Freitas L B.Ecological drivers of plant genetic diversity at the southern edge of geographical distributions: Forestal vines in a temperate region. Genetics & Molecular Biology, 2018, 41(Supple 1): 318-326.
[11] Wang Q, Wang B.The application of DNA molecular markers in fruit tree genetics. Hereditas, 2000, 22(5): 339-344.
王倩, 王斌. DNA分子标记在果树遗传学研究上的应用. 遗传, 2000, 22(5): 339-344.
[12] Alvarez I, Wendel J F.Ribosomal ITS sequences and plant phylogenetic inference. Molecular Phylogenetics & Evolution, 2003, 29(3): 417-434.
[13] Poczai P, Hyvönen J.Nuclear ribosomal spacer regions in plant phylogenetics: Problems and prospects. Molecular Biology Reports, 2010, 37(4): 1897-1912.
[14] Wang Y S, Huang H W, Wang Y.Recent progress in plant molecular population genetics. Hereditas, 2007, 29(10): 1191-1198.
王云生, 黄宏文, 王瑛. 植物分子群体遗传学研究动态. 遗传, 2007, 29(10): 1191-1198.
[15] Zhang D Y, Pan B R, Yin L K.The photogeographical studies of Tamarix (Tamaricaceae). Acta Botanbica Yunnanica, 2003, 25(4): 415-427.
张道远, 潘伯荣, 尹林克. 柽柳科柽柳属的植物地理研究. 云南植物研究, 2003, 25(4): 415-427.
[16] Zhang D Y, Yi L K, Pan B R.A review on the study of salt glands of Tamarix. Acta Botanica Boreali-Occidentalia Sinica, 2003, 23(1): 190-194.
张道远, 尹林克, 潘伯荣. 柽柳泌盐腺结构、功能及分泌机制研究进展. 西北植物学报, 2003, 23(1): 190-194.
[17] Zhang J, Yi L K, Zhang D Y.RAPD analysis of on genetic diversity of natural populations of Tamarix hispida. Acta Botanbica Yunnanica, 2003, 25(5): 557-562.
张娟, 尹林克, 张道远. 刚毛柽柳天然居群遗传多样性初探. 云南植物研究, 2003, 25(5): 557-562.
[18] Zhao J K.Study on genetic structure of Tamarix chinensis in natural populations in Yellow River Delta. Nanjing: Nanjing Forestry University, 2006.
赵景奎. 黄河三角洲柽柳群体遗传多样性的研究. 南京: 南京林业大学, 2006.
[19] Li R.Study on develop of EST-SSR primer and genetic structure of Tamarix chinensis. Nanjing: Nanjing Forestry University, 2007.
李锐. 柽柳 SSR 标记开发及群体遗传结构分析. 南京: 南京林业大学, 2007.
[20] Jiang Z, Chen Y, Bao Y.Population genetic structure of Tamarix chinensis, in the Yellow River Delta, China. Plant Systematics & Evolution, 2012, 298(1): 147-153.
[21] Gaskin J F, Wilson L M.Phylogenetic relationships among native and naturalized Hieracium (Asteraceae) in Canada and the United States based on plastid DNA sequences. Systematic Botany, 2007, 32(2): 478-485.
[22] Taberlet P L, Gielly L, Pautou G, et al. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology, 1991, 17(5): 1105-1109.
[23] Gaskin J F, Schaal B A.Molecular phylogenetic investigation of US invasive Tamarix. Systematic Botany, 2003, 28(1): 86-95.
[24] Zhang M L, Meng H H, Zhang H X, et al. Himalayan origin and evolution of Myricaria (Tamaricaeae) in the Neogene. PLoS One, 2014, 9(6): e97582
[25] He M Z, Zhang K, Tan H J, et al. Nutrient levels within leaves, stems, and roots of the xeric species Reaumuria soongorica in relation to geographical, climatic, and soil conditions. Ecology & Evolution, 2015, 5(7): 1494-1503.
[26] Zhang R H.Study on the genetic variation of Tamarix chinensis populations. Nanjing: Nanjing Forestry University, 2011.
张如华. 柽柳群体遗传变异研究. 南京: 南京林业大学, 2011.
[27] Huang W D, Zhao X Y, Li Y L, et al. Genetic diversity analysis of Caragana microphylla population in different altitude gradients. Pratacultural Science, 2015, 32(4): 552-559.
黄文达, 赵学勇, 李玉霖, 等. 不同海拔梯度下小叶锦鸡儿的居群遗传多样性. 草业科学, 2015, 32(4): 552-559.
[28] Li M, Wang S X, Gao B J.Analysis of genetic diversity of chinese pine (Pinus tabulaeformis) natural secondary forest populations and correlation with theirs habitat ecological factors. Acta Ecologica Sinica, 2013, 33(12): 3602-3610.
李明, 王树香, 高宝嘉. 油松天然次生林居群遗传多样性及与产地地理气候因子的关联分析. 生态学报, 2013, 33(12): 3602-3610.

环境因子对中国柽柳遗传变异的影响

Effects of environmental factors on population genetic diversity of Tamarix chinensis

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