不同稻茬土壤对紫云英根瘤生长特性的影响研究

doi:10.11686/cyxb2019356

草业学报 ›› 2020, Vol. 29 ›› Issue (6): 153-161.DOI: 10.11686/cyxb2019356

不同稻茬土壤对紫云英根瘤生长特性的影响研究

刘芳^1,5, 陈震², 徐雯², 储志英³, 管永祥², 吴桂成⁴, 还静¹, 孙政国^{1,5, *}

1.南京农业大学草业学院,江苏南京 210095;
2.江苏省农业技术推广总站,江苏南京 210036;
3.江苏省武进区雪堰镇农机农技站,江苏武进 213169;
4.昆山市农业技术推广中心,江苏昆山 215300;
5.南京大学(苏州)高新技术研究院,江苏苏州 215123

收稿日期:2019-08-14 修回日期:2020-01-02 出版日期:2020-06-20 发布日期:2020-06-20
通讯作者: * E-mail: sunzg@njau.edu.cn
作者简介:刘芳(1996-),女,陕西神木人,在读硕士。E-mail: liuf0710@126.com
基金资助:
江苏现代农业(水稻)产业技术体系轮作休耕创新团队项目(JATS[2018]217)和苏州市农业科技创新项目(SNG2018081)资助

Impacts of different paddy soils on nodule growth characteristics of Chinese milk vetch

LIU Fang^1,5, CHEN Zhen², XU Wen², CHU Zhi-ying³, GUAN Yong-xiang², WU Gui-cheng⁴, HUAN Jing¹, SUN Zheng-guo^{1,5, *}

1. College of Agro-grassland Science, Nanjing Agricultural University, Nanjing 210095, China;
2. Jiangsu Provincial Agricultural Technology Extension Station, Nanjing 210036, China;
3. Agricultural and Machinery Station of Xueyan Town, Wujin District, Wujin 213169, China;
4. Kunshan Agricultural Technology Promotion Center, Kunshan 215300, China;
5. Nanjing University (Suzhou) High-tech Institute, Suzhou 215123, China

Received:2019-08-14 Revised:2020-01-02 Online:2020-06-20 Published:2020-06-20

摘要/Abstract

摘要： 紫云英根瘤生长特性对不同土壤理化指标变化的响应存在一定的差异性,尤其是在稻茬条件下相关研究较少。为全面分析水稻-紫云英轮作模式下紫云英根瘤生长特性与土壤理化指标之间的关系,试验通过野外调查与室内分析相结合的形式,采取相关分析和通径分析等方法,对苏南太湖流域和宁镇丘陵2个稻作区不同土壤条件下稻茬紫云英根瘤生长特性的差异性进行比较研究。结果表明:不同采样地生长的紫云英单株根瘤数、根瘤重量及固氮酶活性也不同。其中昆山采样地的紫云英单株根瘤数和固氮酶活性均达到最大值(分别为86.24·plant^-1,1362.73 nmol C₂H₄·min^-1·g^-1),而丹阳采样地单株根瘤数和固氮酶活性均最低(分别为1.13·plant^-1,9.25 nmol C₂H₄·min^-1·g^-1);根瘤数量与土壤理化性质的相关性分析,根瘤数量与全氮、有机质、硝态氮极显著相关(P<0.01),与含水量、有效磷显著相关(P<0.05);通过土壤理化性质与根瘤数量的通径分析发现,硝态氮、全氮、有机质、有效磷、土壤pH综合作用依次降低,其中有机质、硝态氮、有效磷的直接通径系数为正值(P₂=1.629,P₁=0.633,P₄=0.448),是促进紫云英结瘤的主要因子,土壤全氮的直接通径系数为负值(P₅=-1.138),是结瘤的主要限制因子。紫云英根瘤生长特性与土壤环境之间存在相互影响、相互协同关系,所以,在实际生产中,稻茬紫云英生长应增施一定数量的有机肥与磷肥,并酌减氮肥用量,以充分提高紫云英结瘤固氮能力。

关键词: 稻茬, 紫云英, 根瘤特性, 有机质, 有效磷, 全氮

Abstract: Nodule growth characteristics of Chinese milk vetch (CMV) differ in response to different soil physical and chemical properties, especially in different paddy soils. This study aimed to analyze the relationship between nodule characteristics of CMV and soil physical and chemical properties in CMV used in crop rotations with rice in Taihu and Ning-zhen rice growing regions. Correlation analysis and path analysis were used to associate differences in nodule characteristics with particular soil conditions in rice stubble. It was found that root nodule number, nodule weight and nitrogenase activity of milk vetch in rice-CMV crop rotations were affected by soil conditions. Nodule number per plant and the nitrogenase activity in Kunshan sample point were high (86.24·plant^-1, 1362.73 nmol C₂H₄·min^-1·g^-1, respectively), while corresponding values for Danyang sample point were low (1.13·plant^-1, 9.25 nmol C₂H₄·min^-1·g^-1, respectively). Path coefficients for nitrate nitrogen, total nitrogen, organic matter and available phosphorus were generally strongly correlated with nodule number, and organic matter, nitrate nitrogen and available phosphorus were positively correlated (P₂=1.629, P₁=0.633, P₄=0.448) but total nitrogen negatively correlated (P₅=-1.138, P represents direct path coefficient of factors). Soil organic matter, nitrate nitrogen and available phosphorus had the greatest positive influence on nodule number, whereas high soil total nitrogen reduced nodule number. There is a synergistic relationship between root nodule characteristics and the soil environment. In practice, appropriate organic fertilizer and phosphate fertilizer should be used to improve the nitrogen fixation capacity in CMV, rather than nitrogen fertilizer.

Key words: rice stubble, Chinese milk vetch, root nodule characteristic, organic matter, available phosphorus, total nitrogen

刘芳, 陈震, 徐雯, 储志英, 管永祥, 吴桂成, 还静, 孙政国. 不同稻茬土壤对紫云英根瘤生长特性的影响研究[J]. 草业学报, 2020, 29(6): 153-161.

LIU Fang, CHEN Zhen, XU Wen, CHU Zhi-ying, GUAN Yong-xiang, WU Gui-cheng, HUAN Jing, SUN Zheng-guo. Impacts of different paddy soils on nodule growth characteristics of Chinese milk vetch[J]. Acta Prataculturae Sinica, 2020, 29(6): 153-161.

参考文献

[1] Sofi P, Wani S. Prospects of nitrogen fixation in rice. Asian Journal of Plant Sciences, 2007, 6(1): 203-213.
[2] Kim M K, Lee Y H, Kang T H, et al. Influence of Chinese milk vetch (Astragalus sinicus) with no-tillage on soil biotic factors and rice yield. Journal of the Korean Society for Applied Biological Chemistry, 2011, 54(6): 899-909.
[3] Haag A F, Arnold M F F, Myka K K, et al. Molecular insights into bacteroid development during Rhizobium-legume symbiosis. Fems Microbiology Reviews, 2013, 37(3): 364-383.
[4] Chalk P M, Craswell E T. An overview of the role and significance of ¹⁵N methodologies in quantifying biological N₂ fixation (BNF) and BNF dynamics in agro-ecosystems. Symbiosis, 2018, 75(1): 1-16.
[5] Cleveland C C, Townsend A R, Schimel D S, et al. Global patterns of terrestrial biological nitrogen (N₂) fixation in natural ecosystems. Global Biogeochemical Cycles, 1999, 13(2): 623-646.
[6] Asagi N, Ueno H. Nitrogen dynamics in paddy soil applied with various ¹⁵N-labelled green manures. Plant and Soil, 2009, 322(1/2): 251-262.
[7] Tang W G, Tang H M, Luo Z Z, et al. Impacts of winter planting patterns on soil heavy metal content and grain quality in late rice in double cropping rice area. Acta Agronomica Sinca, 2011, 37(8): 1457-1464.
汤文光, 唐海明, 罗尊长, 等. 不同种植模式对稻田土壤重金属含量及晚稻稻米品质的影响. 作物学报, 2011, 37(8): 1457-1464.
[8] Lin X J, Cao W D, Wu Y Q, et al. Advance in Astragalus sinicus research. Pratacultural Science, 2011, 28(1): 135-140.
林新坚, 曹卫东, 吴一群, 等. 紫云英研究进展. 草业科学, 2011, 28(1): 135-140.
[9] Du W H, Cao Z Z. Regulating alfalfa (Medicago sativa) vegetative and reproductive growth. Acta Agrestia Sinica, 2005, 13(4): 354-355.
杜文华, 曹致中. 紫花苜蓿营养生长与生殖生长的调控. 草地学报, 2005, 13(4): 354-355.
[10] Su W, Lu J W, Liu W, et al. Effect of N, P and K fertilizer combination and application rate on yield of Astragalus sinicus. Chinese Journal of Eco-Agriculture, 2009, 17(6): 1094-1098.
苏伟, 鲁剑巍, 刘威, 等. 氮磷钾肥用量对紫云英产量效应的研究. 中国生态农业学报, 2009, 17(6): 1094-1098.
[11] Zhou F H. Classification of soil types in Jiangsu Province and its practicalities. Chinese Journal of Soil Science, 1959, 2(5): 30-40.
周傳槐. 江苏省土壤类型的划分及其实用意义. 土壤通报, 1959, 2(5): 30-40.
[12] Shao W J, Song Y X, Wang C, et al. Spatial-temporal variation and associated driving factors of pH values in soils in the past 30 years in the southern Jiangsu Province. Geological Journal of China Universities, 2016, 22(2): 264-273.
邵文静, 宋垠先, 王成, 等. 近30年来苏南耕地土壤pH时空变化特征及影响因素分析. 高校地质学报, 2016, 22(2): 264-273.
[13] Liu W G, Shan L, Deng X P. Responses of plant to soil compaction. Plant Physiology Communications, 2001, 37(3): 254-260.
刘晚苟, 山仑, 邓西平. 植物对土壤紧实度的反应. 植物生理学通讯, 2001, 37(3): 254-260.
[14] Jin J, Wang G H, Liu X B, et al. Root morphology and nodule traits of two soybean varieties on alfisol and mollisol. Chinese Journal of Applied Ecology, 2008, 19(8): 1747-1753.
金剑, 王光华, 刘晓冰, 等. 两个大豆品种在暗棕壤和黑土中的根系形态和根瘤性状. 应用生态学报, 2008, 19(8): 1747-1753.
[15] Brewin N J. Development of the legume root nodule. Annual Review of Cell Biology, 1991, 7(1): 191.
[16] Chen H G. Development of paddy soil characteristics and green manure farming system in paddy fields. Acta Pedologica Sinica, 1955, 3(2): 97-111.
陈华癸. 水稻土特性的发展和水稻田的绿肥耕作制. 土壤学报, 1955, 3(2): 97-111.
[17] Li Y G, Zhou J C. Main factors affecting symbiotic nitrogen fixation efficiency of rhizobium and genetic transformation. Microbiology China, 2002, 29(6): 86-89.
李友国, 周俊初. 影响根瘤菌共生固氮效率的主要因素及遗传改造. 微生物学通报, 2002, 29(6): 86-89.
[18] Yan J, Han X Z, Ding J, et al. Responses of growth, nodulation and yield of soybean to different nitrogen and phosphorus fertilization management. Journal of Plant Nutrition and Fertilize, 2014, 20(2): 318-325.
严君, 韩晓增, 丁娇, 等. 东北黑土区大豆生长、结瘤及产量对氮、磷的响应. 植物营养与肥料学报, 2014, 20(2): 318-325.
[19] Ao Y, Wu Q, Xue P, et al. Research progresses on diversity of quality traits of landrace rice in Taihu Lake region. China Rice, 2016, 22(5): 27-30.
敖雁, 吴启, 薛萍, 等. 太湖地区水稻地方品种品质性状多样性研究进展. 中国稻米, 2016, 22(5): 27-30.
[20] Zhu Y. The study of realtime flood forecasting regulation system in Taihu Basin. Nanjing: Hohai University, 2003.
朱琰. 太湖流域实时洪水预报调度系统研究. 南京: 河海大学, 2003.
[21] Ma T H, Zhu X M, Pan Z R. Study on vulnerability assessment of shallow groundwater in the typical plain area in the Taihu Basin. Ground Water, 2014, 36(6): 53-56.
马天海, 朱晓明, 潘扎荣. 太湖流域典型平原地区浅层地下水脆弱性研究. 地下水, 2014, 36(6): 53-56.
[22] Yuan M M, Liu Q, Zhang S L. Characteristics of nitrogen fixation of winter green manure in paddy soil in the Taihu Lake area. Chinese Journal of Soil Science, 2010, 41(5): 1115-1119.
袁嫚嫚, 刘勤, 张少磊. 太湖地区稻田土壤冬绿肥固氮特性研究. 土壤通报, 2010, 41(5): 1115-1119.
[23] Chen P Q, Chen H Z. Relationship between site conditions and Pinus massoniana growth in low mountains and hilly areas of Ningzhen. Journal of Jiangsu Forestry Science and Technology, 1983, 10(2): 16-21.
陈佩钦, 陈厚照. 宁镇低山丘陵立地条件与马尾松生长的关系. 江苏林业科技, 1983, 10(2): 16-21.
[24] Xu Y L, Li X C. Comprehensive development and utilization of forestry resources in low mountains and hills areas of Ning-zhen-Yang. Journal of Jiangsu Forestry Science and Technology, 1987, 14(2): 1-5.
徐宜良, 李晓储. 宁镇扬低山丘陵林业资源的综合开发利用. 江苏林业科技, 1987, 14(2): 1-5.
[25] Bradstreet R B. Kjeldahl method for organic nitrogen. Analytical Chemistry, 1965, 26(1): 185-187.
[26] Bao S D. Soil agricultural chemistry analysis (Third Edition). Beijing: China Agricultural Press, 2000: 44-49.
鲍士旦. 土壤农化分析(第三版). 北京: 中国农业出版社, 2000: 44-49.
[27] Stewart W D, Fitzgerald G P, Burris R H. In situ studies on N₂ fixation using the acetylene reduction technique. Proceedings of the National Academy of Sciences of the United States of America, 1967, 58(5): 2071-2078.
[28] Wang J, Xu X H. Effects of rhizobium, soybean variety, soil type on nitrogenase activity. Journal of Northeast Agricultural University, 2008, 39(9): 36-39.
王晶, 许修宏. 不同根瘤菌、大豆品种、土壤类型对固氮酶活性的影响. 东北农业大学学报, 2008, 39(9): 36-39.
[29] Xie Z J, Zhou C H, He Y Q, et al. A review of Astragalus sinicus in paddy fields in south China since 2000s. Acta Prataculturae Sinica, 2018, 27(8): 185-196.
谢志坚, 周春火, 贺亚琴, 等. 21世纪我国稻区种植紫云英的研究现状及展望. 草业学报, 2018, 27(8): 185-196.
[30] Liu X Y, Shi F L, Zhang L, et al. Studies on the plant growth and root nodule of different Medicago materials at branching stage. Chinese Journal of Grassland, 2015, 37(3): 116-120.
刘旭艳, 石凤翎, 张璐, 等. 不同苜蓿材料分枝期生长与结瘤的初步研究. 中国草地学报, 2015, 37(3): 116-120.
[31] Yin G L, Li Y J, Zhang Z F, et al. Characteristics of soil nutrients and bacterial community composition under different rotation patterns in grassland. Acta Ecologica Sinica, 2020, 40(5): 1-9.
尹国丽, 李亚娟, 张振粉, 等. 不同草田轮作模式下土壤养分及细菌群落组成特征. 生态学报, 2020, 40(5): 1-9.
[32] Liu Y B. Effects of organic rice-Chinese milk vetch rotation on organic rice yield and soil fertility. Jiangsu Agricultural Sciences, 2014, 42(12): 72-74.
刘亚柏. 有机水稻-红花草轮作对有机稻产量及土壤肥力的影响. 江苏农业科学, 2014, 42(12): 72-74.
[33] Zheng H M, Zhai N N, Mao Y L, et al. Comparison of nodulation competition ability between different strains of Rhizobia isolated from Astragalus sinicus. Jiangsu Agricultural Sciences, 2014, 42(11): 403-406.
郑会明, 翟娜娜, 毛怡玲, 等. 紫云英根瘤菌不同菌株间结瘤竞争能力的比较. 江苏农业科学, 2014, 42(11): 403-406.
[34] Ferguson B J, Indrasumunar A, Hayashi S, et al. Molecular analysis of legume nodule development and autoregulation. Journal of Integrative Plant Biology, 2010, 52(1): 61-76.
[35] David J, Khan K S. Effect of nitrogen application on nodulation in inoculated chickpea (Cicer arietinum). Journal of Biological Sciences, 2001, 1(3): 87-89.
[36] Cun Z X, Zhou Z G, He X H, et al. Regulation of nitrogen and phosphorus fertilizer dosage on nodulation and growth of soybean. Soybean Science, 2014, 33(2): 215-217.
寸植贤, 周志刚, 何霞红, 等. 氮、磷肥用量对大豆结瘤和生长的调节. 大豆科学, 2014, 33(2): 215-217.
[37] Jeong S, Hong J K, Jho E H, et al. Interaction among soil physicochemical properties, bacterial community structure, and arsenic contamination: Clay-induced change in long-term arsenic contaminated soils. Journal of Hazardous Materials, 2019, 378. doi: 10.1016/j.jhazmat.2019.06.006.
[38] Lauber C L, Strickland M S, Bradford M A, et al. The influence of soil properties on the structure of bacterial and fungal communities across land-use types. Soil Biology and Biochemistry, 2008, 40(9): 2407-2415.
[39] Zhao T, Ma C H, Wang D, et al. Correlation between rhizobia distribution and the physical/chemical properties in soil of winter wheat intercropped with sweet clover. Acta Prataculturae Sinica, 2018, 27(4): 45-55.
赵涛, 马春晖, 王栋, 等. 冬小麦套种草木樨土壤中根瘤菌分布与土壤理化性质的相关性分析. 草业学报, 2018, 27(4): 45-55.
[40] Yu T Y, Li X L, Lu Y, et al. Effect of phosphorus (P) on nitrogen (N) uptake and utilization in peanut. Acta Agronomica Sinica, 2019, 45(6): 912-921.
于天一, 李晓亮, 路亚, 等. 磷对花生氮素吸收和利用的影响. 作物学报, 2019, 45(6): 912-921.
[41] Fan J J, Liu X J, Li W Q. Effects of $NO_{3}^{-}$-N/$NH_4^+$-N ratios on root growth and nitrogen-fixation characteristics of alfalfa. Journal of Gansu Agricultural University, 2016, 51(1): 114-119.
范俊俊, 刘晓静, 李文卿. $NO_{3}^{-}$-N/N$NH_4^+$-N配比对紫花苜蓿根系生长及固氮特性的影响. 甘肃农业大学学报, 2016, 51(1): 114-119.
[42] Ye F, Liu X J, Zhang J X. Effects of nitrogen forms on growth, nodulation and nitrogen-fixation of alfalfa at flowering stage. Grassland and Turf, 2014, 34(2): 1-6.
叶芳, 刘晓静, 张进霞. 氮素形态对紫花苜蓿盛花期生长及结瘤固氮的影响. 草原与草坪, 2014, 34(2): 1-6.
[43] Li F L, Li Z P, Liu M, et al. Effects of different concentrations of nitrogen and soil moistures on paddy soil nitrification and microbial characteristics. Chinese Journal of Eco-Agriculture, 2012, 20(9): 1113-1118.
栗方亮, 李忠佩, 刘明, 等. 氮素浓度和水分对水稻土硝化作用和微生物特性的影响. 中国生态农业学报, 2012, 20(9): 1113-1118.
[44] Chen H G, Zhou Q. Studies on nitrification and nitrifying microorganisms in paddy soil Ⅱ. nitrification in paddy soil. Acta Pedologica Sinica, 1961, 9(1/2): 56-64.
陈华癸, 周启. 水稻田土壤中的硝化作用和硝化微生物的研究Ⅱ.水稻田土壤中的硝化作用. 土壤学报, 1961, 9(1/2): 56-64.

不同稻茬土壤对紫云英根瘤生长特性的影响研究

Impacts of different paddy soils on nodule growth characteristics of Chinese milk vetch

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