不同产地白三叶种质遗传多样性的SRAP分析

摘要/Abstract

摘要： 采用SRAP分子标记技术,对不同产地的41份白三叶材料进行遗传多样性分析,筛选出20对随机引物组合,获得以下结果:1)20对随机引物组合共扩增出479条清晰可辨的条带,每对引物组合PCR扩增出13～44条带纹,平均为23.95条,多态性条带411条,多态性位点百分率(PPB)占85.16%,多态性信息含量(PIC)范围为0.182～0.334,平均为0.268,表明供试白三叶种质具有丰富的遗传多样性;2)材料间遗传相似系数(GS)范围为0.5866~0.9896,平均GS值为0.7307;表明供试白三叶种质在分子水平具有相对较远的亲缘关系;3)对所有材料进行聚类分析和主成分分析发现,在GS=0.71的水平上,可将41份白三叶材料分成3大类,大部分来自相同或相似生态地理环境的材料能聚为一类,呈现出一定的生态地理环境分布规律。

Abstract: SRAP (sequence-related amplified polymorphism) markers were used to analyze genetic diversity of 41 white clover (Trifolium repens) accessions using 20 pairs of random primers. The results were: 1) The primer pairs amplified 479 clear bands, including 411 polymorphic bands, which ranged from 13 to 44 bands per primer pair with an average of 23.95 bands. The percent of polymorphic loci was 85.16%, and the polymorphism information content ranged from 0.182 to 0.334 with an average of 0.268, which showed that the tested white clover had a rich genetic diversity of germplasm. 2) Genetic similarity ranged from 0.586 6 to 0.989 6 among all accessions with an average of 0.730 7, which also suggested that there was a rich genetic variation among the white clover germplasms. 3) Cluster analysis and principal component analysis of 41 white clover accessions divided them into 3 groups on the genetic similarity of 0.71 and most of the accessions from the same or similar ecologic geographical environment clustered in one group, showing a certain degree of eco-geographical distribution.

中图分类号:

张婧源,彭燕,罗燕,马啸. 不同产地白三叶种质遗传多样性的SRAP分析[J]. 草业学报, 2010, 19(5): 130-138.

ZHANG Jing-yuan, PENG Yan, LUO Yan, MA Xiao. Genetic diversity in white clover germplasm detected by SRAP markers[J]. Acta Prataculturae Sinica, 2010, 19(5): 130-138.

参考文献

[1] 姜树坤, 马慧, 刘君, 等. 利用SRAP标记分析玉米遗传多样性[J]. 分子植物育种, 2007, 5(3): 412-416.
[2] 张建成, 王传堂, 焦坤, 等. SRAP标记技术在花生种子纯度鉴定中的应用[J]. 中国农学通报, 2005, 21(12): 35-39.
[3] 赵丽萍, 柳李旺, 龚义勤, 等. 萝卜品种指纹图谱SRAP与AFLP分析[J]. 植物研究, 2007, 27(6): 687-684.
[4] Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a newmarker system based on a simple PCR reaction: Its application tomapping and gene tagging in Brassica[J]. Theoretical and Applied Genetics, 2001, 103: 455-461.
[5] Barrett B, Griffiths A, Schreiber M, et al. A microsatellite map of white clover[J]. Theoretical and Applied Genetics, 2004, 109(3): 596-608.
[6] Ferriol M, Pico B, Nuze F. Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers[J]. Theoretical and Applied Genetics, 2003, 107: 271-282.
[7] Han X Y, Wang L S, Shu Q Y, et al. Molecular characterization of tree peony germplasm using sequence-related amplified polymorphism markers[J]. Biochemical Genetics, 2008, 46: 162-179.
[8] Guo D L, Luo Z R. Genetic relationships of some PCNA persimmons (Diospyros kaki Thunb.) from China and Japan revealed by SRAP analysis[J]. Genetic Resources and Crop Evolution, 2006, 53: 1597-1603.
[9] Budak H, Shearman R C, Parmaksiz I, et al. Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers[J]. Theoretical and Applied Genetics, 2004, 108: 328-334.
[10] 陈智华, 苗佳敏, 钟金城. 野生垂穗披碱草种质遗传多样性的SRAP研究[J]. 草业学报, 2009, 18(5): 192-200.
[11] 陈宣, 郭海林, 薛丹丹. 结缕草属植物耐盐性SRAP分子标记研究[J]. 草业学报, 2009, 18(2): 66-75.
[12] 郭海林, 郑轶琦, 陈宣. 结缕草属植物种间关系和遗传多样性的SRAP标记分析[J]. 草业学报, 2009, 18(5): 201-210.
[13] Vandemark G J, Ariss J J, Bauchan G A, et al. Estimating genetic relationships among historical sources of alfalfa germplasm and selected cultivars with sequence related amplified polymorphisms[J]. Euphytica, 2006, 152: 9-16.
[14] 张建成, 王传堂, 焦坤, 等. SRAP标记技术在花生种子纯度鉴定中的应用[J]. 中国农学通报, 2005, 12(21): 35-39.
[15] 董宽虎, 沈益新. 饲草生产学[M]. 北京: 中国农业出版社, 2003: 78-79.
[16] 陈宝书. 牧草饲料作物栽培学[M]. 北京: 中国农业出版社, 2001: 224.
[17] 切彩. 11分白三叶材料形态特征及其同工酶和种子贮藏蛋白遗传多样性研究[D]. 乌鲁木齐: 新疆农业大学, 2009.
[18] 杨珍. 高加索三叶草鱼白三叶草及其杂交胚立体培养技术的研究[D]. 呼和浩特: 内蒙古农业大学, 2008.
[19] 杨珍, 何丽君, 王明玖. 高加索三叶草×白三叶胚萌发条件的探索[J]. 草业科学, 2009, 26(1): 50-54.
[20] Sharmas T R, Singh R K, Chahota R, et al. Analysis of genetic diversity in white clover (Trifolium repens) accessions using agro-morphological and RAPD markers[J]. Journal of Genetics & Breeding, 2005, 59: 3-4.
[21] Gustine D L, Huff D R. Genetic variation within and among white clover populations from managed permanent pastures of the Northeastern USA[J]. Crop Science, 1999, 39: 524-530.
[22] Joyce T A, Abberton M T, Michaelson-Yeates T P T, et al. Relationships between genetic distance measured by RAPD-PCR and heterosis in inbred lines of white clover (Trifolium repens L.)[J]. Euphytica, 1999, 107(3): 159-165.
[23] George J, Dobrowolski M P, Van-Zijll-de-Jong E, et al. Assessment of genetic diversity in cultivars of white clover (Trifolium repens L.) detected by SSR polymorphisms[J]. Genome, 2006, 49: 8.
[24] Klliker R, Hones E S, Jahufer M Z Z, et al. Bulked AFLP analysis for the assessment of genetic diversity in white clover(Trifolium repens L.)[J]. Euphytica, 2001, 121: 305-315.
[25] Saghai-Maroof M A, Soliman K M, Jorgensen R A. Ribosomal DNA pacer-length polymorphisms in barely: Ribosomal inheritance, chromosomal location, and population dynamics[J]. Proceedings of the National Academy of Sciences of the United States of America, 1984, 81: 801.
[26] 王志勇, 袁学军, 刘建秀, 等. 狗牙根SRAP-PCR反应体系优化及引物筛选[J]. 草业学报, 2008, 3(17): 79-85.
[27] 薛丹丹, 郑轶琦, 王志勇, 等. 结缕草属植物SRAP-PCR体系的建立和优化[J]. 草业学报, 2008, 6(17): 93-101.
[28] 易杨杰, 张新全, 黄琳凯, 等. 野生狗牙根种质遗传多样性的SRAP研究[J]. 遗传, 2008, 30(1): 94-100.
[29] 许绍斌, 陶玉芬, 杨昭庆, 等. 简单快速的DNA银染和胶保存方法[J]. 遗传, 2002, 24(3): 335-336.
[30] Roldan-Ruiz I, Dendauw J, Van Bockstaele E, et al. AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.)[J]. Molecular Breeding, 2000, 6: 125-134.
[31] Archak S, Gaikwad A B, Gautam D, et al. Comparative assessment of DNA fingerprinting techniques (RAPD, ISSR and AFLP) for genetic analysis of cashew (Anacardium occidentale L.) accessions of India[J]. Genome, 2003, 46: 362-369.
[32] Powell W, Morgante M, Andre C, et al. The comparison of RAPD, AFLP and SSR(microsatellite) marker for germplasm analysis[J]. Molecular Breeding, 1996, 2: 225-238.
[33] 车永和, 李立会, 何蓓如. 冰草属植物遗传多样性取样策略基于醇溶蛋白的研究[J]. 植物遗传资源学报, 2004, 5: 216-221.
[34] 冯宗云, 李宏, 张立立, 等. 西藏野生大麦醇溶蛋白的遗传多样性[J]. 四川大学学报, 2004, 41: 440-445.
[35] 车永和, 李立会, 何培茹. 冰草属(Agropyron Gaertn.)植物遗传多样性取样策略基于醇溶蛋白的研究[J]. 植物遗传资源学报, 2004, 5: 216-221.
[36] Rohlf F J. NTSYS-pc. Numerial Taxonomy and Multivariate Analysis System (Version 2.0)[M]. New York: Exter Softwere, 1994.
[37] Kongkiatngam P, Waterway M J, Fortin M G, et al. Genetic variation within and between two cultivars of red clover(Trifolium pratense L.)-Comparisons of morphological, isozyme, and RAPD markers[J]. Euphytica, 1995, 84: 237-246.
[38] Ulloa O, Ortega F, Campos H. Analysis of genetic diversity in red clover (Trifolium pratense L.)breeding populations as revealed by RAPD genetic markers[J]. Genome, 2003, 46(6): 529-535.
[39] 关潇. 野生紫花苜蓿种质资源遗传多样性研究[D]. 北京: 北京林业大学, 2009.
[40] Wilson B L, Kitzmiller J, Rolle W, et al. Isozyme variation and its environmental correlates in Elymus glaucus from the California Floristic Province[J]. Canadian Journal of Botany-revue Canadienne de Botanique, 2001, 79: 139-153.
[41] 谢国禄. 北美白三叶草收集种和品种的RAPD标记遗传变异[J]. Crop Science, 2002, 42(2): 342-347.
[42] 安晓珂. 三叶草属三种植物遗传多样性的RAPD分析[D]. 北京: 中国农业科学院, 2008.
[43] Bortolini F, Agnol M D, Schifino-Wittmann M T. Molecular characterization of the USDA white clover (Trifolium repens L.)core collection by RAPD markers[J]. Genetic Resources and Crop Evolution, 2006, 53: 1081-1087