野生新麦草种质醇溶蛋白遗传变异分析

草业学报 ›› 2011, Vol. 20 ›› Issue (4): 311-315.

野生新麦草种质醇溶蛋白遗传变异分析

陈仕勇,马啸,张新全^*,武小龙

四川农业大学动物科技学院草业科学系, 四川雅安 625014

收稿日期:2010-06-04 出版日期:2011-04-25 发布日期:2011-08-20
作者简介:陈仕勇(1984-),男,四川三台人,在读博士。E-mail:chengshi8827@163.com
基金资助:
国家重点基础研究973计划(2007CB108907),国家科技支撑计划(2008BADB3B10)和国家自然科学基金项目(31072077)资助。

Genetic variation analysis of gliadin from wild germplasm of Psathyrostachys juncea

CHEN Shi-yong, MA Xiao, ZHANG Xin-quan, WU Xiao-long

The Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China

Received:2010-06-04 Online:2011-04-25 Published:2011-08-20

摘要/Abstract

摘要： 采用酸性聚丙烯酰胺凝胶电泳技术(A-PAGE)对来自中国新疆、俄罗斯西伯利亚及蒙古等地的35份野生新麦草种质进行醇溶蛋白的遗传变异分析,获得如下结果,1)电泳共检测到37条醇溶蛋白条带,多态性条带比率高达100%;2)种质间遗传相似系数变幅为0~0.827 6,平均值为0.450 9,表明供试材料具有丰富的醇溶蛋白遗传多样性;3)聚类分析将供试材料分成5类,相同或者相似生态地理来源的材料能够聚在一起,表明醇溶蛋白带谱与其地理分布具有一定的相关性;4)种质间的Shannon多样性指数H_t=0.579 6,类群平均多样性指数H_group=0.483 5,其中类群内和类群间的遗传变异分别为83.42%和16.58%,这可能与新麦草异花授粉的繁育方式有关。本研究结果将为新麦草种质资源的保护和利用提供理论依据。

Abstract: Acid polyacrylamide gel electrophoresis (A-PAGE) was used to analyse the gliadin genetic variation among thirty-five wild accessions of Psathyrostachys juncea collected from Xinjiang in China, the Siberia region in Russia, and from Mongolia. 1) A total of 37 bands were obtained from the accessions and all were polymorphic; 2) The Dice genetic similarity coefficient of the tested materials ranged from 0-0.827 6, with a mean of 0.450 9. There was rich genetic polymorphism among the tested wild materials of P. juncea; 3) The 35 wild accessions can be divided into five groups and can be clustered with the same or similar eco-geographical origins. Results also showed some relationship between the patterns of gliadin bands and geographical distribution; 4) Genetic differentiations between and within groups were estimated by Shannon’s diversity index. The results showed H_t=0.579 6 and H_group=0.483 5, which indicated that 83.42% genetic variance existed within groups, and 16.58% genetic variance was between the groups. This might be related to the cross-pollinated breeding system of P. juncea. The results of the study will supply more information for germplasm resources, conservation and breeding of P. juncea.

中图分类号:

陈仕勇,马啸,张新全,武小龙. 野生新麦草种质醇溶蛋白遗传变异分析[J]. 草业学报, 2011, 20(4): 311-315.

CHEN Shi-yong, MA Xiao, ZHANG Xin-quan, WU Xiao-long. Genetic variation analysis of gliadin from wild germplasm of Psathyrostachys juncea[J]. Acta Prataculturae Sinica, 2011, 20(4): 311-315.

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