农杆菌不同菌液浓度对柳枝稷种子萌发及幼苗生长发育的影响

草业学报 ›› 2012, Vol. 21 ›› Issue (1): 206-212.

农杆菌不同菌液浓度对柳枝稷种子萌发及幼苗生长发育的影响

徐开杰, 燕明献, 柴乖强, 孙风丽, 刘曙东, 奚亚军^*

西北农林科技大学农学院,陕西杨凌 712100

出版日期:2012-02-20 发布日期:2012-02-20
通讯作者: E-mail:xiyajun11@126.com
作者简介:徐开杰(1981-),男,山东日照人,在读博士。
基金资助:
国家自然科学基金(30871571),国家948计划项目(2011-G1-19)和西北农林科技大学基本科研业务费专项资金(CX200903)资助。

Switchgrass seeds germination and seedling growth and development with different concentration of Agrobacterium tumefaciens

XU Kai-jie, YAN Ming-xian, CHAI Guai-qiang, SUN Feng-li, LIU Shu-dong, XI Ya-jun

College of Agriculture, Northwest Agriculture and Forestry University, Yangling 712100, China

Online:2012-02-20 Published:2012-02-20

摘要/Abstract

摘要： 以柳枝稷品系‘西稷1号’、‘西稷2号’和‘西稷3号’为主区,以农杆菌菌液浓度为副区,对农杆菌浸种处理后柳枝稷种子萌发及幼苗生长发育相关性状进行了研究。结果表明,品系与农杆菌菌液浓度的互作效应不显著;不同基因型品系对农杆菌的敏感程度不同,其中西稷3号最不敏感;随着农杆菌菌液浓度的升高,柳枝稷种子发芽率、幼苗成苗率及叶绿素含量呈下降趋势,幼苗POD活性、CAT活性、MDA含量呈上升趋势,SOD活性呈先升后降的趋势。综合农杆菌对柳枝稷种子萌发及幼苗生理生化特性影响,在柳枝稷农杆菌浸种转化法中菌液浓度控制在1.2 OD₆₀₀较为适宜。

Abstract: With the switchgrass strains (Xiji No.1, Xiji No.2, Xiji No.3) as the dominated factors, the concentration of Agrobacterium tumefaciens as the deputy factors, the effect of switchgrass seed germination and seedling physio-biochemical characteristics were studied, using A. tumefaciens-soaking method. The results showed that the interaction effect between the strains and the concentration was not significant; The effects of A. tumefaciens on switchgrass strains were different, and the Xiji No.3 was more insensitive to A. tumefaciens than the other strains. With the concentration of A. tumefaciens increased, the switchgrass seed germination rate, the seedling plantingpercent and chlorophyll content showed downtrend, and the seedling POD activity, CAT activity and MDA content showed uptrend, and the seedling SOD activity initially increased but then decreased. To sum up, the most appropiate concentration of A. tumefaciens may be under 1.2 OD₆₀₀ in switchgrass seeds soaking.

中图分类号:

Q945.3

徐开杰, 燕明献, 柴乖强, 孙风丽, 刘曙东, 奚亚军 . 农杆菌不同菌液浓度对柳枝稷种子萌发及幼苗生长发育的影响[J]. 草业学报, 2012, 21(1): 206-212.

XU Kai-jie, YAN Ming-xian, CHAI Guai-qiang, SUN Feng-li, LIU Shu-dong, XI Ya-jun. Switchgrass seeds germination and seedling growth and development with different concentration of Agrobacterium tumefaciens[J]. Acta Prataculturae Sinica, 2012, 21(1): 206-212.

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