不同叶型白三叶抗氧化保护及渗透调节生理对干旱胁迫的响应

摘要/Abstract

摘要： 以大叶型和小叶型白三叶为研究对象,在干旱胁迫及复水条件下,通过测定2种叶型白三叶叶片相对含水量、电导率、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、鸟氨酸转氨酶(OAT)和渗透调节物质(可溶性糖、游离脯氨酸和甜菜碱)等的生理变化,来探讨2种叶型白三叶耐旱机制之间的差异。试验结果表明,随着干旱胁迫时间的延长,2种叶型白三叶叶片相对含水量都呈下降趋势,电导率和丙二醛含量增加,超氧化物歧化酶和过氧化氢酶活性呈先上升后下降趋势,鸟氨酸转氨酶活性急剧升高,渗透调节物质不断积累。但抗旱性较强的小叶型白三叶维持了显著低的膜脂过氧化,显著高的相对含水量、细胞膜稳定性、鸟氨酸转氨酶活性、过氧化氢酶和超氧化物歧化酶活性,渗透调节物质的积累也显著高于大叶型白三叶。复水之后2种叶型白三叶都有不同程度的恢复,但小叶型白三叶恢复较好。

Abstract: Mechanisms of drought tolerance were studied in large-leaved and small-leaved white clovers by measuring physiological changes, including relative water content, electrolyte leakage, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), ornithine aminotransferase (OAT) and osmotic adjustment substances (soluble sugar, free proline and betaine), under drought stress and rewatering. With an extension of drought stress, relative water content of the two materials decreased, while electrolyte leakage and MDA content increased. At the same time, SOD and CAT activities initially increased but then decreased and there was a sharp rise in OAT activity and in content of osmotic adjustment substances. Under drought stress, significantly higher relative water content, cell membrane stability, content of osmotic adjustment substances and activities of SOD, CAT and OAT as well as lower lipid peroxidation level were found in the small-leaved clover leaves compared with those of the large-leaved clover. After re-watering, there were varying degrees of recovery in the two leaf types of white clover, but overall the small-leaved white clover recovered better.

中图分类号:

李州,彭燕,苏星源. 不同叶型白三叶抗氧化保护及渗透调节生理对干旱胁迫的响应[J]. 草业学报, 2013, 22(2): 257-263.

LI Zhou,PENG Yan,SU Xing-yuan. Physiological responses of white clover by different leaf types associated with anti-oxidative enzyme protection and osmotic adjustment under drought stress[J]. Acta Prataculturae Sinica, 2013, 22(2): 257-263.

参考文献

[1] 金忠民, 沙伟. 白三叶抗旱生理的研究[J]. 北方园艺, 2010, 18: 50-52.
[2] 高暝, 李毅, 种培芳, 等. 渗透胁迫下不同地理种源白刺的生理响应[J]. 草业学报, 2011, 20(3): 99-107.
[3] Hendry G A F. Oxygen free radical process and seed longevity[J]. Seed Science Research, 1993, 3: 141-153.
[4] Sairam R K, Vasanthan B, Ajay A. Calcium regulates Gladiolus flower senescence by influencing antioxidative enzymes activity[J]. Acta Physiologiae Plantarum, 2011, 33: 1897-1904.
[5] Mustapha E, Ahmadou M V, Hahib K. Osmoregulation and osmoprotection in the leaf cells of two olive cultivars subjected to severe water deficit[J]. Acta Physiologiae Plantarum, 2009, 31: 711-721.
[6] Lee B R, Li L S, Jung W J, et al. Water deficit-induced oxidative stress and the activation of antioxidant enzymes in white clover leaves[J]. Biologia Plantarum, 2009, 53(3): 505-510.
[7] Wang C Q, Li R C. Enhancement of superoxide dismutase activity in the leaves of white clover (Trifolium repens L.) in response to polyethylene glycol-iduced water stress[J]. Acta Physiologiae Plantarum, 2008, 30: 841-847.
[8] 朱旺生. 干旱胁迫对不同白三叶品种幼苗抗旱性的影响[J]. 草原与饲料, 2010, 30(6): 39-41.
[9] 殷秀杰, 王明玖, 石凤翎, 等. 三种三叶草幼苗期抗旱性差异的研究[J]. 中国草地学报, 2008, 30(2): 68-73.
[10] Irina V, Yasar A, Klimentina D, et al. Drought stress tolerance of red and white clover-comparative analysis of some chaperonins and dehydrins[J]. Scientia Horticulturae, 2011, 130: 653-659.
[11] 钟国成, 张力文, 张利, 等. 不同叶型丹参光合特性研究[J]. 草业学报, 2011, 20(4): 116-122.
[12] Berdour M, Caradus J R, Woodfield D R, et al. Water stress and water use efficiency of ten white clover cultivars[J]. Agronomy Society of New Zealand Special Publication, 1996, 11: 159-162.
[13] Barrs H D, Weatherley P E. A re-examination of the relative turgidity techniques for estimating water deficits in leaves[J]. Australian Journal of Biological Sciences, 1962, 15: 413-428.
[14] Blum A, Ebercon A. Cell membrane stability as a measure of drought and heat tolerance in wheat[J]. Crop Science, 1981, 21: 43-47.
[15] Dhindsa R S, Dhindsa P P, Thorpe T A. Leaf senescence: correlated with increased leaves of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase[J]. Journal of Experimental Botany, 1981, 32: 93-101.
[16] Giannopolities C N, Ries S K. Superoxide dismutase: I. Occurrence in higher plants[J]. Plant Physiology, 1977, 59: 309-314.
[17] Chance B, Maehly A C. Assay of catalase and peroxidase[J]. Methods in Enzymology, 1955, 2: 764-775.
[18] 邹琦. 植物生理学实验指导[M]. 北京: 中国农业出版社, 2000: 161-174.
[19] 王学奎, 章文华, 郝再彬. 植物生理生化原理与技术[M]. 北京: 高等教育出版社, 2006: 202.
[20] 王金贺, 王丽丽, 吴玉梅, 等. 比色法测定饲料甜菜中甜菜碱含量[J]. 中国甜菜糖业, 2008, 1: 5-8.
[21] 余光辉. 水分胁迫下假俭草脯氨酸累积的ABA、Ca²⁺调节[D]. 广州: 华南师范大学, 2003.
[22] 贾利强. 金沙江热河谷造林树种抗旱特性的研究[D]. 北京: 北京林业大学, 2003.
[23] 刘晓军, 洪光宇, 袁志诚, 等. 干热胁迫下两种苇状羊茅对不同水肥处理的响应机理[J]. 草业学报, 2011, 20(1): 46-54.
[24] 邱真静, 李毅, 种培芳. PEG胁迫对不同地理种源沙拐枣生理特性的影响[J]. 草业学报, 2011, 20(3): 108-114.
[25] 张小芸, 何近刚, 孙学辉, 等. 转果聚糖合成关键酶基因多年生黑麦草的获得及抗旱性的提高[J]. 草业学报, 2011, 20(1): 111-118.
[26] 种培芳, 苏世平, 李毅. 4种地理种群红砂的抗旱性综合评价[J]. 草业学报, 2011, 20(5): 26-33.
[27] Hrishikesh U, Sanjib K P, Biman K D. Variation of physiological and antioxidative responses in tea cultivars subjected to elevated water stress followed by rehydration recovery[J]. Acta Physiologiae Plantarum, 2008, 30: 457-468.
[28] 韩建秋. 水分胁迫对白三叶叶片脂质过氧化作用及保护酶活性的影响[J]. 安徽农业科学, 2010, 38(23): 12325-12327.
[29] Raul B, Juan J I, Jesus M S, et al. Short-term drought response of two white clover clones, sensitive and tolerant to O₃[J]. Physiologia Plantarum, 2006, 127: 658-669.
[30] 刘海艳, 齐孝辉, 韩雪. 干旱胁迫及复水对四个白三叶品种叶片保护酶活性的影响[J]. 北方园艺, 2010, 2010(3): 104-106.
[31] Morgan J M. Osmoregulation and water stress in higher plants[J]. Annual Review of Plant Physiology, 1984, 35: 299-319.
[32] 鲍健寅, 杨特武, 冯蕊华, 等. 高温和干旱对白三叶生长发育及生理特性的影响[J]. 草业学报, 1995, 4(4): 9-16.
[33] 杨特武, 鲍健寅, 何光明, 等. 干旱胁迫下白三叶器官生理特性变化及其SOD在抗旱中的作用[J]. 中国草地, 1997, 4: 55-61.
[34] Turner L B. The extent and pattern of osmotic adjustment in white clover (Trifolium repens L.) during the development of water stress[J]. Annals of Botany, 1990, 66(6): 721-727.
[35] Iannucci A, Russo M, Arena L. Water deficit effects on osmotic adjustment and solute accumulation in leaves of annual clovers[J]. European Journal of Agronomy, 2002, 16(2): 111-122.