东祁连山高寒草地几株醉马草内生细菌的生物功能评价及鉴定

doi:10.11686/cyxb20140529

摘要/Abstract

摘要：

采用表面消毒划线法从醉马草分离获得6株内生细菌,分别命名为261MG1、261MG2、261MG3、261MG4、261MY5和261MY6,并对其进行了生物功能测定和鉴定。结果表明,有5株内生细菌对马铃薯炭疽病菌和马铃薯坏疽病菌有较好拮抗作用,有4株对马铃薯枯萎病菌有较好拮抗作用,且261MG2、261MG3、261MG4和261MY6对3种病原菌均有较好拮抗作用;除261MG4外均有固氮和产吲哚乙酸能力;通过培养性状和形态特征,结合16S rDNA序列相似性分析,将261MG1、261MG2、261MG3、261MY6、261MG4和261MY5分别鉴定为枯草芽孢杆菌Bacillus subtilis、Bacillus axarquiensis、莫海威芽孢杆菌Bacillus mojavensis、解淀粉芽孢杆菌Bacillus amyloliquefaciens、耐盐短杆菌Brevibacterium halotolerans和棒形杆菌Clavibacter sp.。该研究结果为醉马草内生细菌进一步开发利用提供了依据。

Abstract:

6 endophytic bacteria of Achnatherum inebrians collected from Alpine grassland on East Qilian Mountains were isolated using a surface disinfection method, and named 261MG1, 261MG2, 261MG3, 261MG4, 261MY5 and 261MY6, respectively. Of these, 5 strains had strong antifungal activity against Colletotrichum coccodes and Phoma foveata, and 4 strains were active against Fusarium avenaceum. In addition, 261MG2, 261MG3, 261MG4 and 261MY6 had inhibitory activity against 3 other pathogens. Except for 261MG4, the endophytic bacteria had the nitrogen-fixing ability and IAA (indole acetic acid) secreting. Based on the culture characteristics, morphological characteristics and 16S rDNA gene sequence analysis, 261MG1, 261MG2, 261MG3, 261MY6, 261MG4 and 261MY5 were identified as Bacillus subtilis, Bacillus axarquiensis, Bacillus mojavensis, Bacillus amyloliquefaciens, Brevibacterium michiganensis, and Clavibacter sp., respectively. The study provides the evidence for the existence of endophytic bacteria of A. inebrians in East Qilian Mountains Alpine grassland, and the initial exploration of their identity and properties.

中图分类号:

S543⁺.901

杨成德,王颖,王玉琴,姚玉玲,薛莉,徐长林,陈秀蓉. 东祁连山高寒草地几株醉马草内生细菌的生物功能评价及鉴定[J]. 草业学报, 2014, 23(5): 249-255.

YANG Cheng-de,WANG Ying,WANG Yu-qin,YAO Yu-ling,XUE Li,XU Chang-lin,CHEN Xiu-rong. Identification and determination of biological functions of endophytic bacteria from Achnatherum inebrians in Alpine grassland of East Qilian Mountains[J]. Acta Prataculturae Sinica, 2014, 23(5): 249-255.

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