根际促生菌筛选及其接种剂对箭筈豌豆生长影响的研究

doi:10.11686/cyxb20140528

摘要/Abstract

摘要：

通过测定分离自箭筈豌豆和玉米根际4株细菌的固氮酶活性、溶磷量及分泌生长素能力,将其制成植物根际接种剂,并结合半固体培养试验测定接种剂对箭筈豌豆生长的影响。结果表明,菌株J3-1、J1-15和Y16具备溶磷和分泌生长素能力,J1-15的溶磷能力最强,为548.9 mg/L,Y16分泌生长素能力最好,达17.8 μg/mL,且菌株Y16具较强固氮能力,J3固氮酶活性为366.51 C₂H₄ nmol/(mL·h)。与对照组相比,单一菌株制备的接种剂处理(Y16)可使箭筈豌豆地上生物量、地下生物量分别显著增加104.5%和254.1%(P<0.05),复合接种剂处理F(J3-1+J1-15+Y16+J3)使箭筈豌豆地上和地下生物量分别增加76.1%和192.3%。综合各指标,复合接种剂处理效果明显优于单一接种剂,处理F(J3-1+J1-15+Y16+J3)可使箭筈豌豆株高、根长、根表面积、根体积、根系活力,分别较对照增加29.4%,70.0%,174.0%,194.6%,38.3%。这主要是由于菌种间的互作效应造成的。

Abstract:

To investigate the effect of rhizobacteria inoculants on the growth of Vicia sativa, four rhizobacteria strains (J3-1, J1-15, Y16 and J3) were assessed for potentially useful characteristics with the potential to enhance the growth and yield of V. sativa; high nitrogenase activity, high phosphate solubilisation and ability to secrete 3-indoleacetic acid (IAA) into the rhizosphere. Strains J3-1, J1-15 and Y16 had the ability to solubilize phosphate and secrete IAA. J1-15, Y16, J3-1 had the strongest phosphate solubilizing capacity (548.9 mg/L), IAA secreting ability (17.8 μg/mL), and nitrogenase activity (366.51 C₂H₄ nmol/mL·h), respectively. Y16 increased the aboveground biomass and underground biomass of V. sativa by 104.5% and 254.1%, respectively, compared to the non-inoculated control. An inoculant mixture of all four strains increased the aboveground biomass and underground biomass of V. sativa by 76.1% and 192.3%, respectively, relative to the control. This resulted from an increase in plant height, root length, root surface area, root volume and root activity of 29.4%, 70.0%, 174.0%, 194.6% and 38.3%, respectively. The results suggest that inoculants including mixtures of rhizobacteria strains may have significant benefits for V. sativa cultivation.

中图分类号:

S816

马文彬,姚拓,王国基,张玉霞,荣良燕. 根际促生菌筛选及其接种剂对箭筈豌豆生长影响的研究[J]. 草业学报, 2014, 23(5): 241-248.

MA Wen-bin,YAO Tuo,WANG Guo-ji,ZHANG Yu-xia,RONG Liang-yan. Assessment of rhizobacteria strains for Vicia sativa[J]. Acta Prataculturae Sinica, 2014, 23(5): 241-248.

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