盐胁迫对新麦草种子萌发及幼苗期生理特性的影响

doi:10.11686/cyxb2018430

摘要/Abstract

摘要： 为研究新麦草种子萌发期及幼苗期的耐盐性,以前期在盐碱地种植的6个不同品种的新麦草为材料,采用0.6%~2.0%的8个浓度梯度NaCl溶液分别处理6种材料,蒸馏水为对照。统计盐胁迫处理下6个品种种子的发芽率、发芽势、根长、芽长等萌发指标。将经1.8%、2.0%高浓度NaCl处理下萌发的幼苗移栽到育苗钵中培育,材料生长到三叶期后,再次进行0.6%浓度NaCl中度盐胁迫,并测定叶片的生长高度,可溶性糖、游离脯氨酸含量,SOD、POD、CAT等抗氧化酶活性及丙二醛含量和电导率。通过隶属函数和主成分分析对6种新麦草萌发期与苗期的耐盐性进行综合评价。结果表明,随着盐浓度的增加,新麦草的发芽率、发芽势、活力指数和相对生长速度均呈下降趋势;幼苗叶片中脯氨酸、可溶性糖、丙二醛的含量和相对电导率在中度盐胁迫后增加;SOD、POD、CAT的变化在不同材料中表现不一致。主成分分析表明盐胁迫下发芽指标是评价新麦草耐盐性的主要成分,其次是抗氧化酶活性和膜质过氧化性。

关键词: 新麦草, 盐胁迫, 种子萌发, 生理特性

Abstract: Salt tolerance of Psathyrostachys juncea at the germination and seedling growth stages was evaluated using 6 different accessions planted in saline-alkali soil. Seeds of the 6 accessions were treated with 0.6%, 0.8%, 1.0%, 1.2%, 1.4%, 1.6%, 1.8% and 2.0% NaCl solutions, and pure water was used as the Control. The germination rate, germination potential, root length and shoot lengths were measured under salt stress. The seedlings germinated under the high 1.8% and 2.0% NaCl solution concentrations were transplanted into nursery pots. After these transplanted seedlings reached the three-leaf stage, 0.6% NaCl solution was added to the growth media, representing medium salt stress. Leaf length was measured. The soluble sugar, free proline, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and other antioxidant enzyme activity and malondialdehyde (MDA) contents were also measured. The general salt tolerance of the six P. juncea accessions at the germination and seedling growth stages was evaluated by subordinate function analysis. The results showed that germination rate, germination potential, seedling vigor index and relative growth rate all decreased with increase in salt concentration. The contents of free proline, soluble sugar, MDA and relative electric conductivity of leaves were increased by exposure to medium salt stress in seedling stage. The changes in SOD, POD and CAT activities varied in different materials. Principal component analysis showed that germination related indexes under salt stress were the key indicators of P. juncea salt tolerance. Antioxidant enzyme activity and MDA level were also identified as salt-tolerance indicators by the second principal component.

Key words: Psathyrostachys juncea, salt stress, seed germination, physiological characteristics

李珍, 云岚, 石子英, 王俊, 张晨, 郭宏宇, 盛誉. 盐胁迫对新麦草种子萌发及幼苗期生理特性的影响[J]. 草业学报, 2019, 28(8): 119-129.

LI Zhen, YUN Lan, SHI Zi-ying, WANG Jun, ZHANG Chen, GUO Hong-yu, SHENG Yu. Physiological characteristics of Psathyrostachys juncea at seed germination and seedling growth stages under salt stress[J]. Acta Prataculturae Sinica, 2019, 28(8): 119-129.

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