转露地菊CgDREB22基因的烟草抗逆性分析

doi:10.11686/cyxb2019552

草业学报 ›› 2020, Vol. 29 ›› Issue (10): 109-118.DOI: 10.11686/cyxb2019552

转露地菊CgDREB22基因的烟草抗逆性分析

黄沁梅¹, 杨伊如¹, 陈丽飞², 尹航¹, 刘贺¹, 刘颖婕¹, 周蕴薇^2,*, 何淼^1,*

1.东北林业大学园林学院,黑龙江哈尔滨 150040;
2.吉林农业大学园艺学院,吉林长春 130118

收稿日期:2019-12-17 出版日期:2020-10-20 发布日期:2020-10-20
通讯作者: *.E-mail: dlzhyw@126.com; hm2017148@126.com
作者简介:黄沁梅(1996-),女,江苏泰州人,在读硕士。E-mail: 1727494083@qq.com
基金资助:
国家自然科学基金项目(31870687)资助

Analysis of stress-resistance of CgDREB22 from Chrysanthemum×grandiflora in tabacco

HUANG Qin-mei¹, YANG Yi-ru¹, CHEN Li-fei², YIN Hang¹, LIU He¹, LIU Ying-jie¹, ZHOU Yun-wei^2,*, HE Miao^1,*

1. College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China;
2. College of Horticulture, Jilin Agricultural University, Changchun 130118, China

Received:2019-12-17 Online:2020-10-20 Published:2020-10-20
Contact: *.E-mail: dlzhyw@126.com; hm2017148@126.com

摘要/Abstract

摘要： DREB转录因子是AP2/ERF转录因子的一个亚家族,主要参与植物对干旱、高盐和低温等逆境的分子应答机制。前期研究从露地菊中分离出一个CgDREB22基因,构建植物过表达载体并用农杆菌介导法转化烟草。对获得的T₁代转基因烟草种子和幼苗进行高盐(150 mmol·L^-1 NaCl )、干旱(150 mmol·L^-1甘露醇)、低温(4 ℃)等处理,进而观察表型并测定相关生理指标。结果表明:在低温、干旱和高盐胁迫下,转基因烟草幼苗鲜重和根长都低于野生型;在高盐和干旱胁迫下,转基因烟草幼苗脯氨酸含量、抗氧化物酶[超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)]活性均显著低于野生型。综上说明,露地菊CgDREB22基因在植物受到非生物胁迫的过程中起负调控的作用。

关键词: 露地菊, CgDREB22, 烟草, 非生物胁迫

Abstract: DREB transcription factor is a subfamily of the AP2/ERF transcription factors, which is involved in the molecular response mechanism of plants to enviromental stresses such as drought, salinity and low temperature. The CgDREB22 gene from Chrysanthemum×grandiflora was incorporated into tobacco using an agrobacterium-mediated method. Seeds and seedlings of the T₁ generation transgenic tobacco were treated with 150 mmol·L^-1 NaCl, 150 mmol·L^-1 mannitol, and low temperature (4 ℃). It was found that under NaCl exposure, osmotically simulated drought stress, and low temperature stress, the fresh weight and root length of transgenic tobacco were lower than in untransformed comparisons. Under NaCl exposure and osmotically simulated drought stress, the resistance mechanism of transgenic tobacco was destroyed,and the proline content and antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) of transgenic tobacco were significantly lower than the untransformed comparison plants. In summary, the CgDREB22 plays a negative role in regulating environmental stresses in plants.

Key words: Chrysanthemum×grandiflora, CgDREB22, tobacco, enviromental stresses

黄沁梅, 杨伊如, 陈丽飞, 尹航, 刘贺, 刘颖婕, 周蕴薇, 何淼. 转露地菊CgDREB22基因的烟草抗逆性分析[J]. 草业学报, 2020, 29(10): 109-118.

HUANG Qin-mei, YANG Yi-ru, CHEN Li-fei, YIN Hang, LIU He, LIU Ying-jie, ZHOU Yun-wei, HE Miao. Analysis of stress-resistance of CgDREB22 from Chrysanthemum×grandiflora in tabacco[J]. Acta Prataculturae Sinica, 2020, 29(10): 109-118.

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转露地菊CgDREB22基因的烟草抗逆性分析

Analysis of stress-resistance of CgDREB22 from Chrysanthemum×grandiflora in tabacco

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