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草业学报 ›› 2021, Vol. 30 ›› Issue (1): 130-139.DOI: 10.11686/cyxb2020070

• 研究论文 • 上一篇    下一篇

外源褪黑素对干旱胁迫下烟草幼苗光合碳同化和内源激素的影响

李冬1,2(), 申洪涛3, 王艳芳1, 王悦华1, 王丽君1, 赵世民4, 刘领1()   

  1. 1.河南科技大学农学院,河南 洛阳 471023
    2.贵州省烟草公司黔东南州公司,贵州 凯里 556000
    3.河南中烟工业有限责任公司技术中心,河南 郑州 450000
    4.河南省烟草公司洛阳市公司技术中心,河南 洛阳 471023
  • 收稿日期:2020-02-21 修回日期:2020-03-23 出版日期:2021-01-20 发布日期:2021-01-08
  • 通讯作者: 刘领
  • 作者简介:Corresponding author. E-mail: liulinghenan@126.com
    李冬(1995-),男,河南洛阳人,在读硕士。E-mail: ldluoyang@126.com
  • 基金资助:
    国家自然基金项目(31700367);洛阳市烟草公司科技攻关项目(LYKJ201803);河南中烟工业有限责任公司资助项目(2020410001340007)

Effects of exogenous melatonin on photosynthetic carbon assimilation and endogenous hormones in tobacco seedlings under drought stress

Dong LI1,2(), Hong-tao SHEN3, Yan-fang WANG1, Yue-hua WANG1, Li-jun WANG1, Shi-min ZHAO4, Ling LIU1()   

  1. 1.College of Agriculture,Henan University of Science and Technology,Luoyang 471023,China
    2.Qiandongnan Branch of Guizhou Tobacco Company,Kaili 556000,China
    3.Technology Center,China Tobacco Henan Industrial Co. ,Ltd. ,Zhengzhou 450000,China
    4.Technology Center,Luoyang Branch of Henan Provincial Tobacco Company,Luoyang 471023,China
  • Received:2020-02-21 Revised:2020-03-23 Online:2021-01-20 Published:2021-01-08
  • Contact: Ling LIU

摘要:

为明确外源褪黑素(MT)对干旱胁迫下烟草幼苗生长缓解的生理机制。以抗旱性不同的两个烤烟品种‘豫烟6号’(Y6,耐旱型)和‘豫烟10号’(Y10,敏感型)为材料,研究了喷施0.2 mmol·L-1外源MT对干旱胁迫下烟草幼苗生长、叶片叶绿素荧光参数、光合碳同化酶活性、碳水化合物和内源激素含量的影响。结果表明,干旱胁迫下,两个烟草品种幼苗的生长均受到不同程度的抑制,且Y10的变化幅度大于Y6;外源褪黑素能有效增加干旱胁迫下烟草幼苗的株高、茎粗、生物量,降低根冠比,提高PSⅡ最大光化学效率(Fv/Fm)、实际光化学效率(ΦPSⅡ)和光化学猝灭系数(qP),降低非光化学猝灭系数(NPQ),提高景天庚酮糖-1, 7-二磷酸酶(SBPase)和核酮糖-1, 5-二磷酸羧化酶(Rubisco)活性,增加可溶性总糖、蔗糖、果糖、生长素(IAA)、赤霉素(GA)和玉米素核苷(ZR)含量,减少淀粉和脱落酸(ABA)含量,且对抗旱性弱的‘豫烟10号’作用效果更强。说明外源MT能够通过增强光合碳同化酶活性,调节叶片内源激素和碳水化合物的代谢,从而缓解干旱胁迫对光合机构的伤害,增强烟草幼苗的抗旱性。

关键词: 褪黑素, 干旱胁迫, 烟草幼苗, 光合碳同化酶, 内源激素, 碳水化合物

Abstract:

In order to elucidate the physiological mechanism of exogenous melatonin (MT) application in relieving drought stress on tobacco seedlings growth. The drought-tolerant variety ‘Yuyan No.6’ and drought-sensitive variety ‘Yuyan No.10’ were tested for their response following foliar-spraying with MT at concentration of 0.2 mmol·L-1 under normal water supply and drought stress. Parameters measured included seedlings growth, chlorophyll fluorescence parameters, photosynthetic carbon assimilation enzyme activity, and the contents of carbohydrates and endogenous hormones of the tobacco seedlings. The results showed that drought stress significantly inhibited the seeding growth of both tobacco cultivars, and the inhibiting effects in drought-sensitive ‘Yuyan No.10’ were greater than that in drought-tolerant ‘Yuyan No.6’. Exogenous melatonin application remarkably increased plant height, stem diameter, biomass, reduced the root-shoot ratio, increased maximal PSⅡ quantum yield (Fv/Fm), PSⅡ actual photochemical efficiency (ΦPSⅡ) and photochemical quenching coefficient (qP), decreased non-photochemical quenching coefficient (NPQ), enhanced the activities of sedoheptulose-1,7-bisphosphatase (SBPase) and ribulose-1,5-bisphosphate carboxylase (Rubisco), and increased the contents of soluble total sugar, sucrose, fructose, auxin (IAA), gibberellin (GA) and zeatin nucleoside (ZR), reduced the contents of starch and abscisic acid (ABA) of tobacco seedlings under drought stress. The changes in drought-sensitive ‘Yuyan No.10’ were greater than those in drought-tolerant ‘Yuyan No.6’. The results indicated that exogenous MT played an important role on enhancing the activity of photosynthetic carbon assimilation enzyme, regulating the metabolism of endogenous hormones and carbohydrates, relieving the damage of photosynthetic apparatus caused by drought stress, and strengthening the drought resistance of tobacco seedlings.

Key words: melatonin, drought stress, tobacco seedlings, photosynthetic carbon assimilation enzyme, endogenous hormones, carbohydrates