草业学报 ›› 2021, Vol. 30 ›› Issue (1): 130-139.DOI: 10.11686/cyxb2020070
李冬1,2(), 申洪涛3, 王艳芳1, 王悦华1, 王丽君1, 赵世民4, 刘领1()
收稿日期:
2020-02-21
修回日期:
2020-03-23
出版日期:
2021-01-20
发布日期:
2021-01-08
通讯作者:
刘领
作者简介:
Corresponding author. E-mail: liulinghenan@126.com基金资助:
Dong LI1,2(), Hong-tao SHEN3, Yan-fang WANG1, Yue-hua WANG1, Li-jun WANG1, Shi-min ZHAO4, Ling LIU1()
Received:
2020-02-21
Revised:
2020-03-23
Online:
2021-01-20
Published:
2021-01-08
Contact:
Ling LIU
摘要:
为明确外源褪黑素(MT)对干旱胁迫下烟草幼苗生长缓解的生理机制。以抗旱性不同的两个烤烟品种‘豫烟6号’(Y6,耐旱型)和‘豫烟10号’(Y10,敏感型)为材料,研究了喷施0.2 mmol·L-1外源MT对干旱胁迫下烟草幼苗生长、叶片叶绿素荧光参数、光合碳同化酶活性、碳水化合物和内源激素含量的影响。结果表明,干旱胁迫下,两个烟草品种幼苗的生长均受到不同程度的抑制,且Y10的变化幅度大于Y6;外源褪黑素能有效增加干旱胁迫下烟草幼苗的株高、茎粗、生物量,降低根冠比,提高PSⅡ最大光化学效率(Fv/Fm)、实际光化学效率(ΦPSⅡ)和光化学猝灭系数(qP),降低非光化学猝灭系数(NPQ),提高景天庚酮糖-1, 7-二磷酸酶(SBPase)和核酮糖-1, 5-二磷酸羧化酶(Rubisco)活性,增加可溶性总糖、蔗糖、果糖、生长素(IAA)、赤霉素(GA)和玉米素核苷(ZR)含量,减少淀粉和脱落酸(ABA)含量,且对抗旱性弱的‘豫烟10号’作用效果更强。说明外源MT能够通过增强光合碳同化酶活性,调节叶片内源激素和碳水化合物的代谢,从而缓解干旱胁迫对光合机构的伤害,增强烟草幼苗的抗旱性。
李冬, 申洪涛, 王艳芳, 王悦华, 王丽君, 赵世民, 刘领. 外源褪黑素对干旱胁迫下烟草幼苗光合碳同化和内源激素的影响[J]. 草业学报, 2021, 30(1): 130-139.
Dong LI, Hong-tao SHEN, Yan-fang WANG, Yue-hua WANG, Li-jun WANG, Shi-min ZHAO, Ling LIU. Effects of exogenous melatonin on photosynthetic carbon assimilation and endogenous hormones in tobacco seedlings under drought stress[J]. Acta Prataculturae Sinica, 2021, 30(1): 130-139.
品种 Variety | 处理 Treatment | 株高 Plant height (cm) | 茎粗 Stem diameter (cm) | 地上部鲜重 Shoot fresh weight (g·plant-1) | 地下部鲜重 Root fresh weight (g·plant-1) | 地上部干重 Shoot dry weight (g·plant-1) | 地下部干重 Root dry weight (g·plant-1) | 根冠比 Root-shoot ratio (DW) |
---|---|---|---|---|---|---|---|---|
Y10 | CK | 4.33±0.13b | 0.84±0.03a | 34.25±0.54b | 6.87±0.30a | 4.02±0.09b | 0.69±0.02a | 0.17±0.01c |
MT | 4.80±0.24a | 0.86±0.01a | 37.53±1.29a | 6.97±0.40a | 4.38±0.04a | 0.70±0.03a | 0.16±0.01c | |
MD | 2.78±0.11d | 0.65±0.01c | 9.75±0.59d | 2.31±0.15c | 1.07±0.06d | 0.24±0.02c | 0.22±0.02a | |
MD+MT | 3.40±0.14c | 0.73±0.01b | 16.39±0.37c | 3.51±0.12b | 1.90±0.03c | 0.36±0.02b | 0.19±0.01b | |
Y6 | CK | 13.65±0.75a | 0.74±0.02a | 24.53±0.87b | 6.88±0.09a | 3.15±0.05b | 0.67±0.02a | 0.21±0.01b |
MT | 13.85±0.19a | 0.77±0.01a | 27.83±0.45a | 6.85±0.18a | 3.32±0.03a | 0.67±0.04a | 0.20±0.01b | |
MD | 10.75±0.22b | 0.64±0.02b | 10.89±0.32d | 3.36±0.21c | 1.55±0.03d | 0.42±0.01c | 0.27±0.01a | |
MD+MT | 11.05±0.21b | 0.68±0.02b | 15.95±0.43c | 4.49±0.17b | 2.04±0.03c | 0.53±0.01b | 0.26±0.01a |
表1 外源MT对干旱胁迫下烟草幼苗生长的影响
Table 1 Effects of exogenous MT on the growth of tobacco seedlings under drought stress
品种 Variety | 处理 Treatment | 株高 Plant height (cm) | 茎粗 Stem diameter (cm) | 地上部鲜重 Shoot fresh weight (g·plant-1) | 地下部鲜重 Root fresh weight (g·plant-1) | 地上部干重 Shoot dry weight (g·plant-1) | 地下部干重 Root dry weight (g·plant-1) | 根冠比 Root-shoot ratio (DW) |
---|---|---|---|---|---|---|---|---|
Y10 | CK | 4.33±0.13b | 0.84±0.03a | 34.25±0.54b | 6.87±0.30a | 4.02±0.09b | 0.69±0.02a | 0.17±0.01c |
MT | 4.80±0.24a | 0.86±0.01a | 37.53±1.29a | 6.97±0.40a | 4.38±0.04a | 0.70±0.03a | 0.16±0.01c | |
MD | 2.78±0.11d | 0.65±0.01c | 9.75±0.59d | 2.31±0.15c | 1.07±0.06d | 0.24±0.02c | 0.22±0.02a | |
MD+MT | 3.40±0.14c | 0.73±0.01b | 16.39±0.37c | 3.51±0.12b | 1.90±0.03c | 0.36±0.02b | 0.19±0.01b | |
Y6 | CK | 13.65±0.75a | 0.74±0.02a | 24.53±0.87b | 6.88±0.09a | 3.15±0.05b | 0.67±0.02a | 0.21±0.01b |
MT | 13.85±0.19a | 0.77±0.01a | 27.83±0.45a | 6.85±0.18a | 3.32±0.03a | 0.67±0.04a | 0.20±0.01b | |
MD | 10.75±0.22b | 0.64±0.02b | 10.89±0.32d | 3.36±0.21c | 1.55±0.03d | 0.42±0.01c | 0.27±0.01a | |
MD+MT | 11.05±0.21b | 0.68±0.02b | 15.95±0.43c | 4.49±0.17b | 2.04±0.03c | 0.53±0.01b | 0.26±0.01a |
品种Variety | 处理Treatment | Fv/Fm | ΦPSⅡ | qP | NPQ |
---|---|---|---|---|---|
Y10 | CK | 0.827±0.009a | 0.482±0.130a | 0.691±0.015a | 1.128±0.099c |
MT | 0.825±0.005a | 0.486±0.040a | 0.681±0.013a | 1.201±0.081c | |
MD | 0.791±0.004c | 0.362±0.009c | 0.529±0.015c | 1.851±0.057a | |
MD+MT | 0.817±0.002b | 0.421±0.005b | 0.635±0.015b | 1.507±0.041b | |
Y6 | CK | 0.831±0.001a | 0.450±0.005a | 0.646±0.011a | 1.333±0.025bc |
MT | 0.830±0.002a | 0.468±0.009a | 0.657±0.006a | 1.235±0.039c | |
MD | 0.809±0.003c | 0.414±0.006b | 0.547±0.005c | 1.678±0.026a | |
MD+MT | 0.829±0.004b | 0.446±0.008a | 0.604±0.007b | 1.426±0.041b |
表2 外源MT对干旱胁迫下烟草幼苗叶片叶绿素荧光参数的影响
Table 2 Effect of exogenous MT on chlorophyll fluorescence parameters in leaves of tobacco seedlings under drought stress
品种Variety | 处理Treatment | Fv/Fm | ΦPSⅡ | qP | NPQ |
---|---|---|---|---|---|
Y10 | CK | 0.827±0.009a | 0.482±0.130a | 0.691±0.015a | 1.128±0.099c |
MT | 0.825±0.005a | 0.486±0.040a | 0.681±0.013a | 1.201±0.081c | |
MD | 0.791±0.004c | 0.362±0.009c | 0.529±0.015c | 1.851±0.057a | |
MD+MT | 0.817±0.002b | 0.421±0.005b | 0.635±0.015b | 1.507±0.041b | |
Y6 | CK | 0.831±0.001a | 0.450±0.005a | 0.646±0.011a | 1.333±0.025bc |
MT | 0.830±0.002a | 0.468±0.009a | 0.657±0.006a | 1.235±0.039c | |
MD | 0.809±0.003c | 0.414±0.006b | 0.547±0.005c | 1.678±0.026a | |
MD+MT | 0.829±0.004b | 0.446±0.008a | 0.604±0.007b | 1.426±0.041b |
图1 外源MT对干旱胁迫下烟草幼苗叶片光合碳同化酶活性的影响不同小写字母表示同一品种不同处理间差异显著(P<0.05)。下同。Different small letters mean significant difference at 0.05 level among different treatments of the same variety. The same below.
Fig.1 Effects of exogenous MT on photosynthetic carbon assimilation enzyme activity in leaves of tobacco seedlings under drought stress
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