草业学报 ›› 2023, Vol. 32 ›› Issue (10): 115-128.DOI: 10.11686/cyxb2022447
• 研究论文 • 上一篇
陈博1(), 牛艺帆1, 刘晓宇1, 张苏晗1, 牛军鹏2, 郭志鹏2, 刘超齐1, 郭永鹏1, 郭玉霞1()
收稿日期:
2022-11-14
修回日期:
2023-02-02
出版日期:
2023-10-20
发布日期:
2023-07-26
通讯作者:
郭玉霞
作者简介:
E-mail: yuxiaguo@163.com基金资助:
Bo CHEN1(), Yi-fan NIU1, Xiao-yu LIU1, Su-han ZHANG1, Jun-peng NIU2, Zhi-peng GUO2, Chao-qi LIU1, Yong-peng GUO1, Yu-xia GUO1()
Received:
2022-11-14
Revised:
2023-02-02
Online:
2023-10-20
Published:
2023-07-26
Contact:
Yu-xia GUO
摘要:
为了探明腐皮镰刀菌对紫花苜蓿种苗的致病性,本研究采用常规组织分离法从苜蓿根部分离纯化真菌,对分离得到的菌株进行形态学结合分子生物学的鉴定,并以菌饼放置种子的方法,分别以水琼脂培养基(water agar, WA)和马铃薯葡萄糖琼脂培养基(potato dextrose agar, PDA)对22个紫花苜蓿品种的种子进行试验,在14 d测定相对根长、相对苗长、相对根苗长度比、相对发芽率和病情指数,并通过隶属函数综合各个指标,对22个苜蓿品种的抗病性进行评价。结果表明:腐皮镰刀菌为导致苜蓿根腐病的病原真菌;在两种培养基质上,腐皮镰刀菌对22个苜蓿品种均有致病性,且在PDA培养基上的致病性强于WA培养基;参试的22个苜蓿品种之间抗病性有所差异,塔苜5S的抗病性最强,中苜1号、Bara310SC、乐寒F3和金皇后的抗病性较强,中兰2号、赛迪7、龙牧803和甘农3号的抗病性较弱,Paola的抗病性最弱。
陈博, 牛艺帆, 刘晓宇, 张苏晗, 牛军鹏, 郭志鹏, 刘超齐, 郭永鹏, 郭玉霞. 腐皮镰刀菌病原致病性及苜蓿品种抗病性研究[J]. 草业学报, 2023, 32(10): 115-128.
Bo CHEN, Yi-fan NIU, Xiao-yu LIU, Su-han ZHANG, Jun-peng NIU, Zhi-peng GUO, Chao-qi LIU, Yong-peng GUO, Yu-xia GUO. An evaluation of the pathogenicity of Fusarium solani and disease resistance of alfalfa varieties[J]. Acta Prataculturae Sinica, 2023, 32(10): 115-128.
序号 Number | 品种 Variety | 秋眠级 FDG | 来源 Source | 育成国家 Breeding country |
---|---|---|---|---|
1 | 金皇后Golden-queen | 2 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 美国America |
2 | 塔苜F6 TARIMU F6 | 6 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 加拿大Canada |
3 | 塔苜5S TARIMU 5S | 5 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 美国America |
4 | 乐寒F4 OPTIMUS F4 | 4 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 加拿大Canada |
5 | 游客Eureka | 8 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 澳大利亚Australia |
6 | WL354HQ | 4 | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd | 美国America |
7 | 乐寒F3 OPTIMUS F3 | 3 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 加拿大Canada |
8 | 中苜1号Zhongmu No.1 | 2 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
9 | 龙牧803 Longmu 803 | 1 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
10 | 塔苜F5 TARIMU F5 | 5 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 中国China |
11 | Bara310SC | 3 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 美国America |
12 | 赛迪7 Sardi 7 | 7 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 澳大利亚Australia |
13 | 甘农9号Gannong No.9 | 7~8 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
14 | 中兰2号Zhonglan No.2 | 3 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
15 | WL363HQ | 5 | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd | 美国America |
16 | Paola | 5 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 意大利Italy |
17 | WL168HQ | 2 | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd | 加拿大Canada |
18 | 大业1号Daye No.1 | 3~5 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
19 | 甘农3号Gannong No.3 | 3 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
20 | 公农1号Gongnong No.1 | 1 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
21 | WL440HQ | 5 | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd | 美国America |
22 | 天马Pegasis | 9 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 澳大利亚Australia |
表1 参试苜蓿品种及其来源
Table 1 Testing alfalfa varieties in the experiment and their sources
序号 Number | 品种 Variety | 秋眠级 FDG | 来源 Source | 育成国家 Breeding country |
---|---|---|---|---|
1 | 金皇后Golden-queen | 2 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 美国America |
2 | 塔苜F6 TARIMU F6 | 6 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 加拿大Canada |
3 | 塔苜5S TARIMU 5S | 5 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 美国America |
4 | 乐寒F4 OPTIMUS F4 | 4 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 加拿大Canada |
5 | 游客Eureka | 8 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 澳大利亚Australia |
6 | WL354HQ | 4 | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd | 美国America |
7 | 乐寒F3 OPTIMUS F3 | 3 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 加拿大Canada |
8 | 中苜1号Zhongmu No.1 | 2 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
9 | 龙牧803 Longmu 803 | 1 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
10 | 塔苜F5 TARIMU F5 | 5 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 中国China |
11 | Bara310SC | 3 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 美国America |
12 | 赛迪7 Sardi 7 | 7 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 澳大利亚Australia |
13 | 甘农9号Gannong No.9 | 7~8 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
14 | 中兰2号Zhonglan No.2 | 3 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
15 | WL363HQ | 5 | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd | 美国America |
16 | Paola | 5 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 意大利Italy |
17 | WL168HQ | 2 | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd | 加拿大Canada |
18 | 大业1号Daye No.1 | 3~5 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
19 | 甘农3号Gannong No.3 | 3 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
20 | 公农1号Gongnong No.1 | 1 | 酒泉大业种业有限责任公司Jiuquan Daye Seed Co., Ltd | 中国China |
21 | WL440HQ | 5 | 北京正道生态科技有限公司Beijing Rytway Ecological Technology Co., Ltd | 美国America |
22 | 天马Pegasis | 9 | 百绿(天津)国际草业有限公司Barenbrug (Tianjin) International Grass Co., Ltd | 澳大利亚Australia |
图1 紫花苜蓿根中分离的腐皮镰刀菌形态特征A为PDA上菌落正面形态,B为PDA上菌落背面形态,C为产孢结构,D为厚垣孢子,E为大分生孢子,F为小分生孢子。A is the frontal morphology of the colony on PDA, B is the back morphology of the colony on PDA, C is conidia, D is chlamydospores, E is macroconidium, F is microconidium.
Fig. 1 Morphological characteristics of F. solani isolated from alfalfa roots
序号Number | 品种 Variety | WA发芽率Germination (%) | PDA发芽率Germination (%) | WA病情指数Disease index | PDA病情指数Disease index | ||||
---|---|---|---|---|---|---|---|---|---|
对照组 Control group | 接菌组 Inoculation group | 对照组 Control group | 接菌组 Inoculation group | 对照组 Control group | 接菌组 Inoculation group | 对照组 Control group | 接菌组 Inoculation group | ||
1 | 金皇后Golden-queen | 100.00±0.00A | 93.00±2.89Aabc | 100.00±8.18A | 96.00±9.28Aa | 0.00±0.00B | 74.00±3.00Abcd | 0.00±0.00B | 81.00±1.73Abcdef |
2 | 塔苜F6 TARIMU F6 | 100.00±2.94A | 86.00±5.08Babcde | 100.00±0.00A | 60.00±8.66Bg | 0.00±0.00B | 70.33±2.89Acde | 0.00±0.00B | 87.67±2.08Aa |
3 | 塔苜5S TARIMU 5S | 100.00±2.94A | 96.00±5.08Aab | 100.00±2.99A | 84.00±2.99Babcde | 0.00±0.00B | 66.00±3.00Ae | 0.00±0.00B | 75.33±5.77Aghi |
4 | 乐寒F4 OPTIMUS F4 | 100.00±5.26A | 84.00±15.79Abcde | 100.00±2.99A | 91.00±11.95Aab | 0.00±0.00B | 72.67±4.04Abcde | 0.00±0.00B | 73.33±2.89Ahi |
5 | 游客Eureka | 100.00±5.97A | 91.00±7.90Aabcd | 100.00±2.94A | 79.00±5.87Bbcde | 0.00±0.00B | 79.00±2.00Aab | 0.00±0.00B | 80.33±0.58Abcdefg |
6 | WL354HQ | 100.00±13.32A | 92.00±11.54Aabcd | 100.00±11.11A | 81.00±8.49Abcde | 0.00±0.00B | 73.33±3.06Abcde | 0.00±0.00B | 80.33±3.21Abcdefg |
7 | 乐寒F3 OPTIMUS F3 | 100.00±2.94A | 88.00±2.94Babcd | 100.00±2.99A | 87.00±5.17Babcd | 0.00±0.00B | 74.67±5.03Aabcd | 0.00±0.00B | 73.00±1.73Ai |
8 | 中苜1号Zhongmu No.1 | 100.00±2.99A | 91.00±5.97Aabcd | 100.00±8.18A | 96.00±10.71Aa | 0.00±0.00B | 72.67±6.51Abcde | 0.00±0.00B | 77.33±2.08Adefghi |
9 | 龙牧803 Longmu 803 | 100.00±0.00A | 91.00±2.89Babcd | 100.00±0.00A | 83.00±7.64Babcde | 0.00±0.00B | 77.67±0.58Aabc | 0.00±0.00B | 83.00±1.00Aabc |
10 | 塔苜F5 TARIMU F5 | 100.00±5.26A | 92.00±3.04Aabc | 100.00±5.26A | 71.00±8.04Befg | 0.00±0.00B | 72.33±3.79Abcde | 0.00±0.00B | 83.00±5.20Aabc |
11 | Bara310SC | 100.00±5.26A | 80.00±3.04Bcdef | 100.00±9.62A | 88.00±5.56Aabc | 0.00±0.00B | 76.67±4.51Aabc | 0.00±0.00B | 78.33±0.58Abcdefghi |
12 | 赛迪7 Sardi 7 | 100.00±9.12A | 70.00±8.04Bf | 100.00±5.97A | 72.00±5.17Befg | 0.00±0.00B | 82.00±4.00Aa | 0.00±0.00B | 82.00±4.00Abcd |
13 | 甘农9号Gannong No.9 | 100.00±2.99A | 84.00±7.90Babcde | 100.00±2.94A | 77.00±2.94Bbcde | 0.00±0.00B | 80.00±2.00Aab | 0.00±0.00B | 75.67±1.15Afghi |
14 | 中兰2号Zhonglan No.2 | 100.00±0.00A | 73.00±7.64Bef | 100.00±5.26A | 77.00±8.04Bcde | 0.00±0.00B | 79.33±1.53Aab | 0.00±0.00B | 76.33±0.58Aefghi |
15 | WL363HQ | 100.00±2.94A | 84.00±5.87Babcde | 100.00±0.00A | 81.00±7.64Bbcde | 0.00±0.00B | 75.33±3.51Aabc | 0.00±0.00B | 80.33±2.89Abcdefg |
16 | Paola | 100.00±0.00A | 70.00±5.00Bf | 100.00±2.94A | 62.00±2.94Bfg | 0.00±0.00B | 77.00±4.58Aabc | 0.00±0.00B | 81.00±3.46Abcdef |
17 | WL168HQ | 100.00±2.94A | 83.00±7.77Bbcdef | 100.00±2.94A | 89.00±2.94Babc | 0.00±0.00B | 73.67±4.93Abcde | 0.00±0.00B | 81.33±1.15Abcde |
18 | 大业1号Daye No.1 | 100.00±3.09A | 80.00±5.36Bcdef | 100.00±3.09A | 75.00±5.36Bdef | 0.00±0.00B | 77.33±5.13Aabc | 0.00±0.00B | 78.67±0.58Abcdefgh |
19 | 甘农3号Gannong No.3 | 100.00±0.00A | 78.00±2.89Bdef | 100.00±5.97A | 72.00±5.17Befg | 0.00±0.00B | 79.67±3.51Aab | 0.00±0.00B | 83.67±2.08Aab |
20 | 公农1号Gongnong No.1 | 100.00±2.94A | 98.00±2.94Aa | 100.00±0.00A | 83.00±2.89Babcde | 0.00±0.00B | 67.33±4.51Ade | 0.00±0.00B | 79.67±1.53Abcdefg |
21 | WL440HQ | 100.00±3.27A | 84.00±5.66Babcde | 100.00±6.30A | 78.00±8.33Bbcde | 0.00±0.00B | 77.33±4.04Aabc | 0.00±0.00B | 78.00±6.00Acdefghi |
22 | 天马Pegasis | 100.00±5.26A | 87.00±12.15Aabcd | 100.00±2.94A | 79.00±2.94Bbcde | 0.00±0.00B | 76.33±6.66Aabc | 0.00±0.00B | 83.00±1.00Aabc |
表2 腐皮镰刀菌对22个紫花苜蓿品种相对发芽率和病情指数的影响
Table 2 Effects of F. solani on relative germination percentage and disease index of 22 alfalfa cultivars
序号Number | 品种 Variety | WA发芽率Germination (%) | PDA发芽率Germination (%) | WA病情指数Disease index | PDA病情指数Disease index | ||||
---|---|---|---|---|---|---|---|---|---|
对照组 Control group | 接菌组 Inoculation group | 对照组 Control group | 接菌组 Inoculation group | 对照组 Control group | 接菌组 Inoculation group | 对照组 Control group | 接菌组 Inoculation group | ||
1 | 金皇后Golden-queen | 100.00±0.00A | 93.00±2.89Aabc | 100.00±8.18A | 96.00±9.28Aa | 0.00±0.00B | 74.00±3.00Abcd | 0.00±0.00B | 81.00±1.73Abcdef |
2 | 塔苜F6 TARIMU F6 | 100.00±2.94A | 86.00±5.08Babcde | 100.00±0.00A | 60.00±8.66Bg | 0.00±0.00B | 70.33±2.89Acde | 0.00±0.00B | 87.67±2.08Aa |
3 | 塔苜5S TARIMU 5S | 100.00±2.94A | 96.00±5.08Aab | 100.00±2.99A | 84.00±2.99Babcde | 0.00±0.00B | 66.00±3.00Ae | 0.00±0.00B | 75.33±5.77Aghi |
4 | 乐寒F4 OPTIMUS F4 | 100.00±5.26A | 84.00±15.79Abcde | 100.00±2.99A | 91.00±11.95Aab | 0.00±0.00B | 72.67±4.04Abcde | 0.00±0.00B | 73.33±2.89Ahi |
5 | 游客Eureka | 100.00±5.97A | 91.00±7.90Aabcd | 100.00±2.94A | 79.00±5.87Bbcde | 0.00±0.00B | 79.00±2.00Aab | 0.00±0.00B | 80.33±0.58Abcdefg |
6 | WL354HQ | 100.00±13.32A | 92.00±11.54Aabcd | 100.00±11.11A | 81.00±8.49Abcde | 0.00±0.00B | 73.33±3.06Abcde | 0.00±0.00B | 80.33±3.21Abcdefg |
7 | 乐寒F3 OPTIMUS F3 | 100.00±2.94A | 88.00±2.94Babcd | 100.00±2.99A | 87.00±5.17Babcd | 0.00±0.00B | 74.67±5.03Aabcd | 0.00±0.00B | 73.00±1.73Ai |
8 | 中苜1号Zhongmu No.1 | 100.00±2.99A | 91.00±5.97Aabcd | 100.00±8.18A | 96.00±10.71Aa | 0.00±0.00B | 72.67±6.51Abcde | 0.00±0.00B | 77.33±2.08Adefghi |
9 | 龙牧803 Longmu 803 | 100.00±0.00A | 91.00±2.89Babcd | 100.00±0.00A | 83.00±7.64Babcde | 0.00±0.00B | 77.67±0.58Aabc | 0.00±0.00B | 83.00±1.00Aabc |
10 | 塔苜F5 TARIMU F5 | 100.00±5.26A | 92.00±3.04Aabc | 100.00±5.26A | 71.00±8.04Befg | 0.00±0.00B | 72.33±3.79Abcde | 0.00±0.00B | 83.00±5.20Aabc |
11 | Bara310SC | 100.00±5.26A | 80.00±3.04Bcdef | 100.00±9.62A | 88.00±5.56Aabc | 0.00±0.00B | 76.67±4.51Aabc | 0.00±0.00B | 78.33±0.58Abcdefghi |
12 | 赛迪7 Sardi 7 | 100.00±9.12A | 70.00±8.04Bf | 100.00±5.97A | 72.00±5.17Befg | 0.00±0.00B | 82.00±4.00Aa | 0.00±0.00B | 82.00±4.00Abcd |
13 | 甘农9号Gannong No.9 | 100.00±2.99A | 84.00±7.90Babcde | 100.00±2.94A | 77.00±2.94Bbcde | 0.00±0.00B | 80.00±2.00Aab | 0.00±0.00B | 75.67±1.15Afghi |
14 | 中兰2号Zhonglan No.2 | 100.00±0.00A | 73.00±7.64Bef | 100.00±5.26A | 77.00±8.04Bcde | 0.00±0.00B | 79.33±1.53Aab | 0.00±0.00B | 76.33±0.58Aefghi |
15 | WL363HQ | 100.00±2.94A | 84.00±5.87Babcde | 100.00±0.00A | 81.00±7.64Bbcde | 0.00±0.00B | 75.33±3.51Aabc | 0.00±0.00B | 80.33±2.89Abcdefg |
16 | Paola | 100.00±0.00A | 70.00±5.00Bf | 100.00±2.94A | 62.00±2.94Bfg | 0.00±0.00B | 77.00±4.58Aabc | 0.00±0.00B | 81.00±3.46Abcdef |
17 | WL168HQ | 100.00±2.94A | 83.00±7.77Bbcdef | 100.00±2.94A | 89.00±2.94Babc | 0.00±0.00B | 73.67±4.93Abcde | 0.00±0.00B | 81.33±1.15Abcde |
18 | 大业1号Daye No.1 | 100.00±3.09A | 80.00±5.36Bcdef | 100.00±3.09A | 75.00±5.36Bdef | 0.00±0.00B | 77.33±5.13Aabc | 0.00±0.00B | 78.67±0.58Abcdefgh |
19 | 甘农3号Gannong No.3 | 100.00±0.00A | 78.00±2.89Bdef | 100.00±5.97A | 72.00±5.17Befg | 0.00±0.00B | 79.67±3.51Aab | 0.00±0.00B | 83.67±2.08Aab |
20 | 公农1号Gongnong No.1 | 100.00±2.94A | 98.00±2.94Aa | 100.00±0.00A | 83.00±2.89Babcde | 0.00±0.00B | 67.33±4.51Ade | 0.00±0.00B | 79.67±1.53Abcdefg |
21 | WL440HQ | 100.00±3.27A | 84.00±5.66Babcde | 100.00±6.30A | 78.00±8.33Bbcde | 0.00±0.00B | 77.33±4.04Aabc | 0.00±0.00B | 78.00±6.00Acdefghi |
22 | 天马Pegasis | 100.00±5.26A | 87.00±12.15Aabcd | 100.00±2.94A | 79.00±2.94Bbcde | 0.00±0.00B | 76.33±6.66Aabc | 0.00±0.00B | 83.00±1.00Aabc |
图3 WA培养基上腐皮镰刀菌对22个紫花苜蓿品种生长的影响横坐标苜蓿品种从左往右依次是:金皇后、塔苜F6、塔苜5S、乐寒F4、游客、WL354HQ、乐寒F3、中苜1号、龙牧803、塔苜F5、Bara310SC、赛迪7、甘农9号、中兰2号、WL363HQ、Paola、WL168HQ、大业1号、甘农3号、公农1号、WL440HQ、天马。纵坐标为相对苗长和相对根长。不同小写字母表示差异显著(P<0.05),下同。Alfalfa varieties from left to right respectively are: Golden-queen, TARIMU F6, TARIMU 5S, OPTIMUS F4, Eureka, WL354HQ, OPTIMUS F3, Zhongmu No.1, Longmu 803, TARIMU F5, Bara310SC, Sardi 7, Gannong No.9, Zhonglan No.2, WL363HQ, Paola, WL168HQ, Daye No.1, Gannong No.3, Gongnong No.1, WL440HQ, Pegasis. The ordinate is relative seedling length and relative root length, different lowercase letters indicate significant differences (P<0.05), the same below.
Fig.3 Effects of F. solani on the growth of 22 alfalfa cultivars in WA medium
序号 Number | 品种 Variety | WA | PDA | 综合评价值 D value | 排名 Rank | ||
---|---|---|---|---|---|---|---|
对照组 Control group | 接菌组 Inoculation group | 对照组 Control group | 接菌组 Inoculation group | ||||
1 | 金皇后Golden-queen | 1.00±0.16A | 0.80±0.18Bbcd | 1.00±0.23A | 0.44±0.13Bdefg | 0.588 | 5 |
2 | 塔苜F6 TARIMU F6 | 1.00±0.14A | 0.80±0.20Bbcd | 1.00±0.20A | 0.46±0.12Bbcdef | 0.497 | 9 |
3 | 塔苜5S TARIMU 5S | 1.00±0.13A | 0.89±0.25Bab | 1.00±0.15A | 0.54±0.15Bab | 0.760 | 1 |
4 | 乐寒F4 OPTIMUS F4 | 1.00±0.16A | 0.64±0.17Be | 1.00±0.22A | 0.45±0.11Bcdefg | 0.470 | 13 |
5 | 游客Eureka | 1.00±0.12A | 0.74±0.20Bde | 1.00±0.19A | 0.47±0.14Bbcdef | 0.508 | 8 |
6 | WL354HQ | 1.00±0.14A | 0.64±0.16Be | 1.00±0.12A | 0.56±0.20Ba | 0.488 | 11 |
7 | 乐寒F3 OPTIMUS F3 | 1.00±0.24A | 0.93±0.30Aa | 1.00±0.19A | 0.52±0.11Babc | 0.609 | 4 |
8 | 中苜1号 Zhongmu No.1 | 1.00±0.19A | 0.77±0.21Bcd | 1.00±0.19A | 0.45±0.11Bcdefg | 0.641 | 2 |
9 | 龙牧803 Longmu 803 | 1.00±0.16A | 0.76±0.17Bcd | 1.00±0.16A | 0.46±0.12Bbcdef | 0.348 | 20 |
10 | 塔苜F5 TARIMU F5 | 1.00±0.15A | 0.87±0.23Babc | 1.00±0.23A | 0.51±0.18Babcd | 0.463 | 15 |
11 | Bara310SC | 1.00±0.18A | 0.81±0.18Bbcd | 1.00±0.22A | 0.43±0.11Befg | 0.613 | 3 |
12 | 赛迪7 Sardi 7 | 1.00±0.17A | 0.78±0.17Bcd | 1.00±0.18A | 0.41±0.17Bfg | 0.352 | 19 |
13 | 甘农9号Gannong No.9 | 1.00±0.17A | 0.78±0.26Bcd | 1.00±0.18A | 0.39±0.12Bfg | 0.410 | 17 |
14 | 中兰2号Zhonglan No.2 | 1.00±0.19A | 0.71±0.15Bde | 1.00±0.16A | 0.40±0.15Bfg | 0.362 | 18 |
15 | WL363HQ | 1.00±0.18A | 0.63±0.16Be | 1.00±0.24A | 0.41±0.10Bfg | 0.477 | 12 |
16 | Paola | 1.00±0.18A | 0.77±0.15Bcd | 1.00±0.17A | 0.41±0.13Bfg | 0.275 | 22 |
17 | WL168HQ | 1.00±0.14A | 0.76±0.16Bcd | 1.00±0.17A | 0.41±0.09Bfg | 0.469 | 14 |
18 | 大业1号Daye No.1 | 1.00±0.21A | 0.64±0.16Be | 1.00±0.22A | 0.46±0.13Bbcdef | 0.491 | 10 |
19 | 甘农3号Gannong No.3 | 1.00±0.15A | 0.76±0.16Bcd | 1.00±0.15A | 0.43±0.10Bdefg | 0.278 | 21 |
20 | 公农1号Gongnong No.1 | 1.00±0.12A | 0.78±0.16Bbcd | 1.00±0.16A | 0.37±0.08Bg | 0.524 | 7 |
21 | WL440HQ | 1.00±0.18A | 0.70±0.15Bde | 1.00±0.19A | 0.51±0.24Babcd | 0.427 | 16 |
22 | 天马Pegasis | 1.00±0.17A | 0.78±0.19Bbcd | 1.00±0.20A | 0.42±0.10Bfg | 0.537 | 6 |
表3 腐皮镰刀菌对22个紫花苜蓿品种相对根苗长度比的影响及综合评价
Table 3 Effects of F. solani on relative root-shoot ratio of 22 alfalfa cultivars and comprehensive evaluation
序号 Number | 品种 Variety | WA | PDA | 综合评价值 D value | 排名 Rank | ||
---|---|---|---|---|---|---|---|
对照组 Control group | 接菌组 Inoculation group | 对照组 Control group | 接菌组 Inoculation group | ||||
1 | 金皇后Golden-queen | 1.00±0.16A | 0.80±0.18Bbcd | 1.00±0.23A | 0.44±0.13Bdefg | 0.588 | 5 |
2 | 塔苜F6 TARIMU F6 | 1.00±0.14A | 0.80±0.20Bbcd | 1.00±0.20A | 0.46±0.12Bbcdef | 0.497 | 9 |
3 | 塔苜5S TARIMU 5S | 1.00±0.13A | 0.89±0.25Bab | 1.00±0.15A | 0.54±0.15Bab | 0.760 | 1 |
4 | 乐寒F4 OPTIMUS F4 | 1.00±0.16A | 0.64±0.17Be | 1.00±0.22A | 0.45±0.11Bcdefg | 0.470 | 13 |
5 | 游客Eureka | 1.00±0.12A | 0.74±0.20Bde | 1.00±0.19A | 0.47±0.14Bbcdef | 0.508 | 8 |
6 | WL354HQ | 1.00±0.14A | 0.64±0.16Be | 1.00±0.12A | 0.56±0.20Ba | 0.488 | 11 |
7 | 乐寒F3 OPTIMUS F3 | 1.00±0.24A | 0.93±0.30Aa | 1.00±0.19A | 0.52±0.11Babc | 0.609 | 4 |
8 | 中苜1号 Zhongmu No.1 | 1.00±0.19A | 0.77±0.21Bcd | 1.00±0.19A | 0.45±0.11Bcdefg | 0.641 | 2 |
9 | 龙牧803 Longmu 803 | 1.00±0.16A | 0.76±0.17Bcd | 1.00±0.16A | 0.46±0.12Bbcdef | 0.348 | 20 |
10 | 塔苜F5 TARIMU F5 | 1.00±0.15A | 0.87±0.23Babc | 1.00±0.23A | 0.51±0.18Babcd | 0.463 | 15 |
11 | Bara310SC | 1.00±0.18A | 0.81±0.18Bbcd | 1.00±0.22A | 0.43±0.11Befg | 0.613 | 3 |
12 | 赛迪7 Sardi 7 | 1.00±0.17A | 0.78±0.17Bcd | 1.00±0.18A | 0.41±0.17Bfg | 0.352 | 19 |
13 | 甘农9号Gannong No.9 | 1.00±0.17A | 0.78±0.26Bcd | 1.00±0.18A | 0.39±0.12Bfg | 0.410 | 17 |
14 | 中兰2号Zhonglan No.2 | 1.00±0.19A | 0.71±0.15Bde | 1.00±0.16A | 0.40±0.15Bfg | 0.362 | 18 |
15 | WL363HQ | 1.00±0.18A | 0.63±0.16Be | 1.00±0.24A | 0.41±0.10Bfg | 0.477 | 12 |
16 | Paola | 1.00±0.18A | 0.77±0.15Bcd | 1.00±0.17A | 0.41±0.13Bfg | 0.275 | 22 |
17 | WL168HQ | 1.00±0.14A | 0.76±0.16Bcd | 1.00±0.17A | 0.41±0.09Bfg | 0.469 | 14 |
18 | 大业1号Daye No.1 | 1.00±0.21A | 0.64±0.16Be | 1.00±0.22A | 0.46±0.13Bbcdef | 0.491 | 10 |
19 | 甘农3号Gannong No.3 | 1.00±0.15A | 0.76±0.16Bcd | 1.00±0.15A | 0.43±0.10Bdefg | 0.278 | 21 |
20 | 公农1号Gongnong No.1 | 1.00±0.12A | 0.78±0.16Bbcd | 1.00±0.16A | 0.37±0.08Bg | 0.524 | 7 |
21 | WL440HQ | 1.00±0.18A | 0.70±0.15Bde | 1.00±0.19A | 0.51±0.24Babcd | 0.427 | 16 |
22 | 天马Pegasis | 1.00±0.17A | 0.78±0.19Bbcd | 1.00±0.20A | 0.42±0.10Bfg | 0.537 | 6 |
图5 接种腐皮镰刀菌后的苜蓿苗的长势及病情指数分级标准A为WA培养基上对照组,B为WA培养基实验组,C为PDA培养基上对照组,D为PDA培养基上实验组,E为病情指数分级标准。从左到右是0~V级。A is the control group on WA medium, B is the experimental group on WA medium, C is the control group on PDA medium, D is the experimental group on PDA medium, E is the disease index grading standard. From left to right, it’s 0-V.
Fig. 5 The growth and grading standard of disease index of alfalfa seedlings inoculated with F. solani
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