镉胁迫对白三叶的富集能力、叶片显微结构及其生理特性的影响

doi:10.11686/cyxb20140621

摘要/Abstract

摘要： 以白三叶为试验材料,对其进行不同浓度Cd²⁺(0, 100, 200, 300, 400, 500 μmol/L)胁迫7 d,结果表明,随Cd²⁺浓度增加,白三叶各营养器官Cd积累量随之升高,各器官对Cd的富集能力表现为:根>叶>茎;同时,富集在叶片部分的Cd对白三叶叶片产生了毒害作用,使叶肉细胞的细胞壁着色加深,栅栏组织细胞以及海绵组织细胞的形状、大小及细胞排列的疏密程度发生改变。Cd²⁺胁迫后,白三叶叶片叶绿素含量显著降低(P<0.05),叶片的MDA含量呈上升趋势,且与Cd²⁺浓度之间存在剂量效应关系;整个胁迫过程中,SOD,POD和CAT活性则呈先升高后降低的趋势。相关性分析结果表明,Chl a和Chl b含量与Cd²⁺浓度间呈极显著负相关;MDA含量与Cd²⁺浓度间呈极显著正相关;SOD,POD,CAT之间呈极显著正相关,三者发挥协同作用以抵抗逆境毒害,体现了白三叶对重金属Cd的适应性。

Abstract: Cadmium (Cd) toxicity was investigated in Trifolium repens using accumulation ability microstructure physiological. T. repens were treated with Cd²⁺(0, 100, 200, 300, 400, 500 μmol/L) for 7 days. Cd accumulation in different organs of T. repens gradually increased with the increase in the concentration of Cd. The accumulation content of different organs followed the sequence: roots>leaves>stems. Meanwhile, Cd accumulation in the leaves produced certain toxicity: cell walls of mesophyll cell stained deepening, the shape, size, and density were also changed. In response to Cd²⁺, more MDA and ROS contents were produced in leaves than untreated of T. repens. Compared with untreated, the photosynthetic pigments contents decreased significantly (P<0.05). After Cd²⁺ treated, the content of MDA generally increased, and there is a dose-response relationship between the concentration of Cd. Throughout the process of stress, SOD, POD, CAT activity increased initially and then decreased. Correlation analysis showed that: the content of Chl a and Chl b were significantly negatively correlated with Cd²⁺ concentration. While the content of MDA was highly significant positively correlated with Cd²⁺ concentration. The activity of SOD, POD and CAT were highly significant positively among each other. Above three play synergistic role to reduce injury. These physiological indicators reflect T. repens adaptability to Cd²⁺ stress.

中图分类号:

S816

韩宝贺,朱宏. 镉胁迫对白三叶的富集能力、叶片显微结构及其生理特性的影响[J]. 草业学报, 2014, 23(6): 167-175.

HAN Bao-he,ZHU Hong. Effects of cadmium stress on accumulation ability, microstructure and physiological property in leaves of Trifolium repens[J]. Acta Prataculturae Sinica, 2014, 23(6): 167-175.

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