草业学报 ›› 2014, Vol. 23 ›› Issue (5): 143-152.DOI: 10.11686/cyxb20140516
潘明洪1,凌瑶2,景文1,马洪平1,彭燕1*
收稿日期:
2014-03-31
出版日期:
2014-10-20
发布日期:
2014-10-20
通讯作者:
Email:pengyanlee@163.com
作者简介:
潘明洪(1988-),男,四川乐山人,在读硕士。E-mail:www.pmh68@163.com
基金资助:
四川省科技支撑项目(No.2013NZ0013)和十二五农村领域国家科技计划课题(No.2011BAD17B03)资助
PAN Ming-hong1,LING Yao2,JING Wen1,MA Hong-ping1,PENG Yan1
Received:
2014-03-31
Online:
2014-10-20
Published:
2014-10-20
摘要:
为阐明四川部分地区野生白三叶根瘤菌的遗传多样性及系统发育地位,对分离自四川雅安、康定、泸定、西昌、成都和乐山6个地区白三叶根瘤的69株菌进行系统研究。采用16S rDNA限制性片段长度多态性分析(restriction fragment length polymorphism, RFLP)和16S rDNA基因、持家基因(recA、atpD、glnII)、结瘤基因(nodC)、固氮基因(nifH)系统发育分析的方法进行了研究。结果表明,16S rDNA PCR-RFLP中所有供试菌株产生了4种酶切图谱类型,表现出较为丰富的遗传多样性。持家基因与16S rDNA基因系统发育分析结果基本一致,9株代表菌株主要分布在α-变形菌纲(Alpha-Proteobacteria)的根瘤菌属(Rhizobium),并与豌豆根瘤菌三叶草生物型(R. leguminosarum bv. trifolii) ATCC 14480T的亲缘关系较近。PCR可扩增出nodC和nifH基因片段,但从属于土壤杆菌属(Agrobacterium)的菌株LS1105中则扩增不出这两个基因。所有供试菌株被鉴定到了种的水平,证实了68株为白三叶根瘤菌,并通过不同采样地点菌株之间的比较,发现白三叶与根瘤菌的共生关系因地理分布不同而具有多样性,对于丰富白三叶根瘤菌资源及其开发利用具有重要意义。
中图分类号:
潘明洪,凌瑶,景文,马洪平,彭燕. 四川白三叶根瘤菌遗传多样性及系统发育研究[J]. 草业学报, 2014, 23(5): 143-152.
PAN Ming-hong,LING Yao,JING Wen,MA Hong-ping,PENG Yan. Genetic diversity and phylogeny of rhizobia isolated from white clover in Sichuan Province[J]. Acta Prataculturae Sinica, 2014, 23(5): 143-152.
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