高寒生态脆弱区“黑土滩”草地植被与土壤微生物数量特征研究

doi:10.11686/cyxb20140525

摘要/Abstract

摘要：

为从微观视野认识“黑土滩”草地退化机理及为该类型草地改良和恢复提供理论依据和科技支撑,本研究以甘肃省天祝县高寒草地为例,测定分析了草地植被、土壤三大类微生物(真菌、细菌和放线菌)和氮素生理群(氨化细菌、硝化细菌、反硝化细菌、好气性和嫌气性固氮菌)在空间层次和不同季节的数量变化。结果表明:“黑土滩”草地植被以微孔草和香薷为优势种,其盖度和植物量较低;微生物主要分布在0~20 cm,三大类微生物数量以细菌最多、真菌最少,分别占总数的84.60%和0.17%;氮素生理群数量以好气性固氮菌最多、嫌气性固氮菌最少,分别占总数的57.51%和0.05%。微生物数量表现出明显的季节动态,8月份达到最大值,除土壤细菌(10月)之外,真菌、放线菌和各氮素生理群微生物最小值都出现在4月份。土壤氮素含量、草地植物量均与硝化细菌、好气性固氮菌数量呈正相关关系,而与反硝化细菌数量呈负相关关系,并且都达到了显著水平(P<0.05)。

Abstract:

In order to better define the soil dynamics of the black beach soil, in grassland dynamics and provide theory and techniques for grassland restoration informed by microbiology, we researched the soil of molehills in an ecologically vulnerable alpine region of Tianzhu County, Gansu Province. We detected and analyzed the microbial population of 3 main categories of soil microorganisms (fungi, bacteria and actinomycetes) and the bacteria groups involved in nitrogen transformation (ammonifiers, nitrifiers, denitrifying bacteria, aerobic and anaerobic nitrogen fixing bacteria). Taxa were cataloged by soil depth and season of sample collection using plate colony count and MPN methods. In addition, the above-ground plant biomass and total Soil N content were investigated by regular methods. Key results are: the dominant species in molehill grassland was Microula sikkimensis, the vegetation coverage and above-ground plant biomass was low. The seasonal dynamic of microbial population was well defined. The maximum number of taxa of fungi, bacteria, actinomycetes and N-transforming bacteria was observed in the 0-20 cm soil layer in August, and the minimum in the 20-40 cm layer in April, except for the ammonifier and aerobic N-fixing soil bacteria for which the number was observed in October. There was a positive correlation between both soil N and plant biomass and the microbial population of nitrifiers and aerobic N-fixing bacteria. By contrast, there was a negative correlation between soil N and denitrifying bacteria.

中图分类号:

S812.2

卢虎,李显刚,姚拓,蒲小鹏. 高寒生态脆弱区“黑土滩”草地植被与土壤微生物数量特征研究[J]. 草业学报, 2014, 23(5): 214-222.

LU Hu,LI Xian-gang,YAO Tuo,PU Xiao-peng. Characteristics of vegetation and soil microorganisms of molehill grassland in an ecologically vulnerable alpine region[J]. Acta Prataculturae Sinica, 2014, 23(5): 214-222.

参考文献

Reference:

[1] Li X L. Natural factors and formative mechanism of "Black Beach" formed on grassland in Qinghai-Tibetan plateau[J]. Pratacultural Science, 2002, 19(1): 20-22. 

[2] Ma Y T, Shang Z H, Shi J J, et al. Studies on communities diversity and their structure of "black-soil-land" degraded grassland in the headwater of Yellow River[J]. Pratacultural Science, 2006, 23(12): 6-11.

[3] Li X L, Huang B N. The cause of "Black Soil Patch" grassland in Qinghai Province and management countermeasures[J]. Grassland of China, 1995, 4: 64-67, 51.

[4] Liu W X, Ma S, Liu G P. Countermeasures of "Black Beach" in Tianjun County[J]. Qinghai Prataculture, 2010, 19(1): 21-24.

[5] Cheng X Y, Hou Y, Ren G H, et al. Allelopathic effects of aqueous extracts from "Black Soil Patch"poisonous weeds on elymus nutans in Degraded Alpine Meadow[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(10): 2057-2064.

[6] Li S X, Ma Y S, Wang Y L, et al. Study on seasonal dynamic of soil seed bank of artificial grassland in Black Soil Beach[J]. Chinese Qinghai Journal of Animal and Veterinary Sciences, 2011, 41(5): 4-6.

[7] Wang Y L, Ma Y S, Shi J J, et al. Dynamics of biomass and nutrition of mixed-sown pasture at 'black soil beach' in Yellow River headwater area[J]. Pratacultural Science, 2010, 27(5): 19-22.

[8] Li X L. Effect of resowing grasses on resuming vegetation of "Black Soil Patch"[J]. Pratacultural Science, 1996, 13(5): 17-19.

[9] Chen S Y, Liu W J, Ye B S, et al. Species diversity of vegetation in relation to biomass and environmental factors in the upper area of the Shule River[J]. Acta Prataculturae Sinica, 2011, 20(3): 70-83.

[10] Yao T, Long R J. Dynamics of soil microbial population under disturbance in Tianzhu Alpine grassland[J]. Acta Prataculturae Sinica, 2006, 15(4): 93-96.

[11] Yao T, Long R J, Shi S L, et al. Populations of soil nitrogen bacteria groups in alpine steppe of different disturbed habitats in Tianzhu [J]. Acta Pedologica Sinica, 2007, 44(1): 122-129.

[12] Xiao F J, Ouyang H. Ecosystem health and its evolution indicator and method[J]. Journal of Natural Resources, 2002, 17(2): 203-209.

[13] Gao Q Z, Duan M J, Li Y E, et al. Change trends of grassland coverage and pasture growth in the Northwest Area of Tibet[J]. Chinese Journal of Agrometeorology, 2010, 31(4): 582-585.

[14] Zhang Y X, Li X B, Chen Y H. Overview of field and multi-scale remote sensing measurement approaches to grassland vegetation coverage[J]. Advance in Earth Sciences, 2003, 18(1): 85-93.

[15] Fan Y G, Hu Y K, Li K H, et al. Effects of Different Disturbances on the Diversity and Biomass of the Phytobiocoenoses in Alpine Steppes[J]. Arid Zone Research, 2008, 25(4): 531-536.

[16] Chinese Academy of Sciences Institute of Nanjing Soil Science microbes room. Soil microbial research method[M]. Beijing: Science Press, 1985.

[17] Xu G H, Zheng H Y. Soil microbial analysis methods manual[M]. Beijing: Agricultural Press, 1986.

[18] Yao H Y, Huang C Y. Soil microbial ecology and experimental techniques[M]. Beijing: Science Press, 2007.

[19] Lu R K. Agricultural chemical analysis of the soil[M]. Beijing: China Agricultural Science and Technology Press, 2000: 147-195.

[20] Wang Y B, Wang G X, Wu Q B, et al. The impact of vegetation degeneration on hydrology features of alpine soil[J]. Journal of Glaciology and Geocryology, 2010, 32(5): 989-998.

[21] Wang S J, Su H, Gao L. The preliminary research on soil microorganism quantity at Kubuqi Sandland[J]. Chinese Journal of Grassland, 2008, (6): 91-95.

[22] Ding L L, Qi B, Shang Z H, et al. The characteristics of soil microorganism quantity under different alpine grasslands in eastern Qilian Mountain[J].Journal of Agro-Environment Science, 2007, (6): 106-113

[23] Gao X X, Man B Y, Chen X R, et al. Identification and determination of biological characteristics of Kobresia capillifolia endophytic bacteria X4 in the East Qilian Mountain Alpine grasslands[J]. Acta Prataculturae Sinica, 2013, 22(4): 137-146.

[24] Gu L J, Xu B L, Liang Q L, et al. Impact and colonisation ability of Trichoderma biocontrol on lawn soil microflora[J]. Acta Prataculturae Sinica, 2013, 22(3): 321-326.

[25] Grundmann G L, Debouzie D. Geostatistical analysis of the distribution of NH4+ and NO2 oxidizing bacteria and serotypes at the millimeter scale along a soil transect[J]. FEMS Microbial Ecology, 2000, 34: 57-62.

[26] Zhang C B. Study on the seasonal dynamics model of soil microbial growth on the leymus chinensis meadow in the Northeast of China[J]. Acta Agrestia Sinica, 2002, 10(1): 134-138.

[27] Eviner V T, Chapin III F S. Selective gopher disturbance influences plant species effects on nitrogen cycling[J]. OIKOS, 2005, 109: 154-166.

[28] Tresserras R M C, lvarez M T S. Role of small mammal disturbances in mountain grasslands[J]. PASTOS, 2004, 1: 47-60.

[29] Canals R M, Eviner V T, Herman D J, et al. Plant colonizers shape early N-dynamics in gopher mounds[J]. Plant and Soil, 2005, 276: 327-334.

[30] Cao L, Qin S H, Zhang J L, et al. Effect of leguminous forage rotations on soil microbe consortiums and enzyme activity in continuously cropped potato fields[J]. Acta Prataculturae Sinica, 2013, 22(3): 139-145.

[31] Dinesman L G. Influence of vertebrates on primary production of terrestrial communities[A]. In: Petrusewica K. Secondary Productivity of Terrestrial Ecosystems[M]. Minnesota, 1967: 261-266.

[32] Ross B A, Tester J R, Breckenridge W J. Ecology of mima-type mounds in north-western Minnesota[J]. Ecology, 1968, 49: 172-177. 

[33] Zlotin R E, Kodashova K S. The Role of Animals in the Biological Cycle of the Forest steppe Ecosystem[M]. Moscow: Science Publishing House, 1974.

[34] Wearn J A, Gange A C. Above ground herbivory causes rapid and sustained changes in mycorrhizal colonization of grasses[J]. Oecologia, 2007, 153: 959-971.

参考文献：

[1] 李希来. 青藏高原“黑土滩”形成的自然因素与生物学机制[J]. 草业科学, 2002, 19(1): 20-22. 

[2] 马玉寿, 尚占环, 施建军, 等. 黄河源区“黑土滩”退化草地群落类型多样性及其群落结构研究[J]. 草业科学, 2006, 23(12): 6-11.

[3] 李希来, 黄葆宁. 青海黑土滩草地成因及治理途径[J]. 中国草地, 1995, 4: 64-67, 51.

[4] 刘维香, 马寿, 刘贵萍. 天峻县黑土滩成因及治理对策[J]. 青海草业, 2010, 19(1): 21-24.

[5] 程晓月, 后源, 任国华, 等. “黑土滩”退化高寒草地6种常见毒杂草水浸液对垂穗披碱草的化感作用[J]. 西北植物学报, 2011, 31(10): 2057-2064.

[6] 李世雄, 马玉寿, 王彦龙, 等. 黑土滩人工草地土壤种子库季节动态研究[J]. 青海畜牧兽医杂志, 2011, 41(5): 4-6.

[7] 王彦龙, 马玉寿, 施建军, 等. 黄河源区“黑土滩”混播草地牧草植物量及营养动态初探[J]. 草业科学, 2010, 27(5): 19-22.

[8] 李希来. 补播禾草恢复“ 黑土滩” 植被的效果[J]. 草业科学, 1996, 13(5): 17-19.

[9] 陈生云, 刘文杰, 叶柏生, 等. 疏勒河上游地区植被物种多样性和生物量及其与环境因子的关系[J]. 草业学报, 2011, 20(3): 70-83.

[10] 姚拓, 龙瑞军. 天祝高寒草地不同扰动生境土壤三大类微生物数量动态研究[J]. 草业学报, 2006, 15(4): 93-96.

[11] 姚拓, 龙瑞军, 师尚礼, 等. 高寒草地不同扰动生境土壤微生物氮素生理群数量特征研究[J]. 土壤学报, 2007, 44(1): 122-129.

[12] 肖风劲, 欧阳华. 生态系统健康及其评价指标和方法[J]. 自然资源学报, 2002, 17(2): 203-209.

[13] 高清竹, 段敏杰, 李玉娥, 等. 1981-2006年藏西北地区草地植被盖度动态变化分析[J]. 中国农业气象, 2010, 31(4): 582-585.

[14] 张云霞, 李晓兵, 陈云浩. 草地植被盖度的多尺度遥感与实地测量方法综述[J]. 地球科学进展, 2003, 18(1): 85-93.

[15] 范永刚, 胡玉昆, 李凯辉, 等. 不同干扰对高寒草原群落物种多样性和生物量的影响[J]. 干旱区研究, 2008, 25(4): 531-536.

[16] 中国科学院南京土壤研究所微生物室. 土壤微生物研究法[M]. 北京: 科学出版社, 1985.

[17] 许光辉, 郑洪元. 土壤微生物分析方法手册[M]. 北京: 农业出版社, 1986.

[18] 姚槐应, 黄昌勇. 土壤微生物生态学及其实验技术[M]. 北京: 科学出版社, 2007.

[19] 鲁如坤. 土壤农业化学分析方法[M]. 北京: 中国农业科技出版社, 2000: 147-195.

[20] 王一博, 王根绪, 吴青柏, 等. 植被退化对高寒土壤水文特征的影响[J]. 冰川冻土, 2010, 32(5): 989-998.

[21] 王素娟, 苏和, 高丽. 库布齐沙地土壤微生物数量初步研究[J].中国草地学报, 2008, (6): 91-95.

[22] 丁玲玲, 祁彪, 尚占环, 等. 东祁连山不同高寒草地型土壤微生物数量分布特征研究[J].农业环境科学学报, 2007, (6): 106-113

[23] 高晓星, 满百膺, 陈秀蓉, 等. 东祁连山线叶嵩草内生细菌X4的产吲哚乙酸、解磷、抗菌和耐盐特性研究及分子鉴定[J]. 草业学报, 2013, 22(4): 137-146.

[24] 古丽君, 徐秉良, 梁巧兰, 等. 生防木霉对草坪土壤微生物区系的影响及定殖能力研究[J]. 草业学报, 2013, 22(3): 321-326.

[25] Grundmann G L, Debouzie D. Geostatistical analysis of the distribution of NH4+-and NO2-oxidizing bacteria and serotypes at the millimeter scale along a soil transect[J]. FEMS Microbial Ecology, 2000, 34: 57-62.

[26] 张崇邦. 东北羊草草原土壤微生物生长动态模型的研究[J]. 草地学报, 2002, 10(1): 134-138.

[27] Eviner V T, Chapin III F S. Selective gopher disturbance influences plant species effects on nitrogen cycling[J]. OIKOS, 2005, 109: 154-166.

[28] Tresserras R M C, lvarez M T S. Role of small mammal disturbances in mountain grasslands[J]. PASTOS, 2004, 1: 47-60.

[29] Canals R M, Eviner V T, Herman D J, et al. Plant colonizers shape early N-dynamics in gopher-mounds[J]. Plant and Soil, 2005, 276: 327-334.

[30] 曹莉, 秦舒浩, 张俊莲, 等. 轮作豆科牧草对连作马铃薯田土壤微生物菌群及酶活性的影响[J]. 草业学报, 2013, 22(3): 139-145.

[31] Dinesman L G. Influence of vertebrates on primary production of terrestrial communities[A]. In: Petrusewica K. Secondary Productivity of Terrestrial Ecosystems[M]. Minnesota, 1967: 261-266.

[32] Ross B A, Tester J R, Breckenridge W J. Ecology of mima-type mounds in north-western Minnesota[J]. Ecology, 1968, 49: 172-177. 

[33] Zlotin R E, Kodashova K S. The Role of Animals in the Biological Cycle of the Forest-steppe Ecosystem[M]. Moscow: Science Publishing House, 1974.

[34] Wearn J A, Gange A C. Above-ground herbivory causes rapid and sustained changes in mycorrhizal colonization of grasses[J]. Oecologia, 2007, 153: 959-971.