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草业学报 ›› 2013, Vol. 22 ›› Issue (5): 183-189.DOI: 10.11686/cyxb20130521

• 研究论文 • 上一篇    下一篇

羊草种子休眠和萌发的激素调控研究

黄晓辉,胡小文*,徐宗海,王彦荣   

  1. 兰州大学草地农业科技学院 草地农业生态系统国家重点实验室, 甘肃 兰州 730020
  • 出版日期:2013-10-20 发布日期:2013-10-20
  • 通讯作者: E-mail:huxw@lzu.edu.cn
  • 作者简介:黄晓辉(1987-),女,内蒙古阿左旗人,在读硕士。
  • 基金资助:
    甘肃省科技重大专项计划(1203FKDA035)和国家自然科学基金(31001030,30771532)资助。

Effects of exogenous hormones on the dormancy and germination of Leymus chinensis

HUANG Xiao-hui, HU Xiao-wen, XU Zong-hai, WANG Yan-rong   

  1. College of Pastoral Agriculture Science and Technology, Lanzhou University, State Key Laboratory of Grassland Agro-ecosystems, Lanzhou 730020, China
  • Online:2013-10-20 Published:2013-10-20

摘要: 采用脱落酸(ABA)、赤霉素3(GA3)、赤霉素4+7(GA4+7)3种外源激素和氟啶酮(fluridone,FL)、多效唑(paclobutrazol,PA)2种激素生物合成抑制剂处理羊草种子,研究其对羊草种子休眠和萌发的影响,结果表明,低浓度外源ABA对羊草种子休眠和萌发无影响,高浓度显著抑制其萌发;ABA的生物合成抑制剂FL可逆转ABA对萌发的抑制作用,表明吸胀过程中ABA的合成是维持羊草种子休眠的重要原因;GA4+7 显著促进羊草种子萌发,而GA3没有影响;GA的生物合成抑制剂PA可显著降低种子萌发率,但其抑制作用可为外源GA3所逆转,说明GA对于非休眠种子的萌发是必须的;FL+PA处理后,尽管吸胀过程中ABA的合成受到抑制,但种子的萌发率仍显著降低,进一步支持GA是种子萌发所必需的结论。除FL溶液冷层积8周可显著破除羊草种子休眠外,其他介质冷层积均不同程度的诱导种子形成二次休眠,这一过程可能与冷层积中ABA含量的增加有关。

Abstract: To study the effects of plant hormones on the seed dormancy and germination of Leymus chinensis, three exogenous hormones including Abscisic acid (ABA), Gibberellin 3 (GA3), Gibberellin 4+7 (GA4+7) and their biosynthetic inhibitors were used to treat seeds. Seed germination was not affected by low exogenous ABA concentrations, but was significantly reduced at the highest concentration. This reduction was reversed by FL application indicating that ABA biosynthesis may play a critical role in maintenance of seed dormancy. The type of gibberellin affected its effect: GA4+7 significantly promoted seed germination, but GA3 had no effect. Percentage seed germination significantly decreased after immersion in PA which is a biosynthesis inhibitors of GA, but this effect can be reversed by exogenous GA3. This shows that GA is necessary for non-dormant seed germination. This was further confirmed by a combination of PA plus FL treatments, which inhibited ABA biosynthesis during imbibition, although the percentage seed germination still decreased. In addition, 8 weeks cold stratification in FL can significantly break seed dormancy of L. chinensis, but other media can induce secondary dormancy, a process that may be related to an increase of ABA during imbibition.

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