盐胁迫对菘蓝幼苗生长和抗性生理的影响

doi:10.11686/cyxb2017282

摘要/Abstract

摘要： 以“定蓝1号”为试验材料,采用不同浓度NaCl溶液(0,30,60,90,120和150 mmol·L^-1)对菘蓝幼苗进行处理,胁迫14 d后测定了其生长指标、生物量积累、叶片相对含水量、根系活力、根质膜透性、渗透调节物质含量以及抗氧化酶活性等指标,分析不同程度盐胁迫对菘蓝幼苗生长和抗性生理的影响。结果表明:NaCl胁迫对菘蓝幼苗株高和根长都有抑制作用,对根和叶的物质积累也有显著影响,并随浓度的递增呈降低的趋势;菘蓝幼苗叶片相对含水量和根系活力均随NaCl浓度的增加呈现下降趋势,而根质膜透性、叶片脯氨酸含量则呈升高趋势;丙二醛(MDA)含量、组织自动氧化速率(RAD)、过氧化物酶(POD)活性和超氧化物歧化酶(SOD)活性都随NaCl浓度的增加呈先升高后降低的趋势。但当NaCl浓度低于90 mmol·L^-1时,菘蓝幼苗的生长没有受到盐胁迫的明显抑制,根系活力降低不明显,脯氨酸、丙二醛含量和保护酶活性也未显著升高,说明菘蓝幼苗对低浓度盐环境具有一定的耐受性。

关键词: 菘蓝, 盐胁迫, 幼苗生长, 根系活力, 生理响应

Abstract: The effects of different concentrations of NaCl solution (0, 30, 60, 90, 120 and 150 mmol·L^-1) on the growth, biomass, leaf relative water content, root activity, root membrane permeability, osmotic adjustment and protective enzyme activity of Isatis indigotica seedlings (cv. Dinglan No.1) were assessed to determine the response of growth and physiological traits associated with resistance to salt stress after 14 days of exposure. Plant height, root length, root dry weight and leaf dry weight of I. indigotica seedlings under NaCl treatment were significantly reduced compared with the control (no salt). Reductions were greater under higher salt concentrations. As NaCl concentration increased leaf relative water content and root activity declined while root membrane permeability and leaf proline content increased. Superoxide dismutase (SOD) and peroxidase (POD) activity, malondialdehyde (MDA) content and rates of tissue autoxidation (RAD) all initially increased at lower salt levels and then declined. However seedling growth was not significantly inhibited under salt stress and root activity showed no obviously decrease. Malondialdehyde and proline content, SOD and POD activity were not affected by NaCl concentration <90 mmol·L^-1. This study showed that I. indigotica seedlings had some tolerance to low salt stress.

Key words: Isatis indigotica, salt tolerance, seedling growth, root activity, physiological responses

米永伟, 王国祥, 龚成文, 蔡子平, 武伟国. 盐胁迫对菘蓝幼苗生长和抗性生理的影响[J]. 草业学报, 2018, 27(6): 43-51.

MI Yong-wei, WANG Guo-xiang, GONG Cheng-wen, CAI Zi-ping, WU Wei-guo. Effects of salt stress on growth and physiology of Isatis indigotica seedlings[J]. Acta Prataculturae Sinica, 2018, 27(6): 43-51.

参考文献

[1] Zhang Y F, Liang Z W, Sui L, et al. Effect on physiological characteristic of Medicago sativa under saline-alkali stress at seeding stage. Acta Prataculturae Sinica, 2009, 18(4): 230-235.
张永锋, 梁正伟, 隋丽, 等. 盐碱胁迫对苗期紫花苜蓿生理特性的影响. 草业学报, 2009, 18(4): 230-235.
[2] State Pharmacopoeia Commission.People’s Republic of China pharmacopoeia (one). Beijing: Chinese Medical Science and Technology Press, 2015: 21,199,205.
国家药典委员会. 中华人民共和国药典(一部). 北京: 中国医药科技出版社,2015: 21,199,205.
[3] Hu S P, Ding T, Zhang D W, et al. The antimicrobial effect of the compound tablet and its effect on immune function. Pharmacology and Clinics of Chinese Materia Medica, 2007, 23(5): 188.
胡淑平, 丁涛, 张殿文, 等. 复方板蓝根含片的抗菌作用及对免疫功能的影响. 中药药理与临床, 2007, 23(5): 188.
[4] Chen Y, Li X, Chen J W, et al. Study on antiviral effects of the active parts from Radix Isatidis. Journal of Nanjing University of Chinese Medicine, 2011, 27(2): 155-157.
程妍, 李祥, 陈建伟, 等. 板蓝根有效部位的抗病毒药效研究. 南京中医药大学学报, 2011, 27(2): 155-157.
[5] Ma Y M, Li N, Liu C W, et al. Comparative studies on anti-inflammatory and analgesic activities of different fractions from Radix Isatidis. Chinese Traditional and Herbal Drugs, 2014, 45(17): 2517-2521.
马毅敏, 李娜, 刘承伟, 等. 板蓝根不同提取部位抗炎镇痛活性比较研究. 中草药, 2014, 45(17): 2517-2521.
[6] Duan F, Yang J X, Zhou X K, et al. Effects of stress on the indirubin content of Isatis indigotica seedlings. Agricultural Research in the Arid Areas, 2006, 24(3): 111-114.
段飞, 杨建雄, 周西坤, 等. 逆境胁迫对菘蓝幼苗靛玉红含量的影响. 干旱地区农业研究, 2006, 24(3): 111-114.
[7] Wang Z C, Liang Z S, Ding Y H.Effect of water stress on growth and physiological characteristics of Isatis indigotica root. Acta Agriculturae Boreali-occidentalis Sinica, 2010, 19(12): 98-103.
王竹承, 梁宗锁, 丁永华. 水分胁迫对菘蓝幼苗生长和生理特性的影响. 西北农业学报, 2010, 19(12): 98-103.
[8] Chen Y H, Tian H Q, Guo Q S, et al. Effects of various planting densities on dynamic growth rhythm and root yield of Isatis indigotica. China Journal of Chinese Materia Medica, 2008, 33(22): 2599-2602.
陈宇航, 田汉卿, 郭巧生, 等. 种植密度对菘蓝生长动态及产量的影响. 中国中药杂志, 2008, 33(22): 2599-2602.
[9] Xiao Y H, Lü T T, Tang X Q, et al. Effects of topdressing nitrogen on apparent quality, dry matter accumulation and contents of active components in the root of Isatis indigotica. Journal of Plant Nutrition and Fertilizer, 2014, 20(2): 437-444.
肖云华, 吕婷婷, 唐晓清, 等. 追施氮肥量对菘蓝根的外形品质、干物质积累及活性成分含量的影响. 植物营养与肥料学报, 2014, 20(2): 437-444.
[10] Wang X L, Chen M H, Wang F, et al. Chemical consitituents from root of Isatis indigotica. China Journal of Chinese Materia Medica, 2013, 38(8): 1172-1182.
王晓良, 陈明华, 王芳, 等. 板蓝根水提取物的化学成分研究. 中国中药杂志, 2013, 38(8): 1172-1182.
[11] Huang F, Xiong Y T, Xu L H, et al. Pharmacokinetics of epigoitrin, an anti-virus component of Isatis indigotica, in different extraction in rats. Journal of China Pharmaceutical University, 2006, 37(6): 519-522.
黄芳, 熊雅婷, 徐丽华, 等. 板蓝根不同提取物中抗病毒成分表告依春在大鼠体内的药代动力学. 中国药科大学学报, 2006, 37(6): 519-522.
[12] Liu P, Tian C Y, Feng G, et al. Germination and seedling survival after desiccation of three Salsola L. from the solonchak salinities. Arid Land Geography, 2008, 31(2): 271-277.
刘鹏, 田长彦, 冯固, 等. 三种猪毛菜种子耐盐性与幼苗干燥存活能力. 干旱区地理, 2008, 31(2): 271-277.
[13] Guo F X, Chen Y, Li X R, et al. Enhancement of low-temperature tolerance in watermelon (Citrullus lanatus) seedlings by cool-hardening germination. Australian Journal of Experimental Agriculture, 2007, 47(6): 749-754.
[14] Zou Q.Instructional on plant physiology. Beijing: China Agriculture Press, 2007.
邹琦. 植物生理学实验指导. 北京:中国农业出版社, 2007.
[15] You J, Wang W R, Lu J, et al. Effects of salinity on seed germination and seedling growth in halophyte Limonium aureum (L.) Hill. Acta Ecologica Sinica, 2012, 32(12): 3825-3833.
尤佳, 王文瑞, 卢金, 等. 盐胁迫对盐生植物黄花补血草种子萌发和幼苗生长的影响. 生态学报, 2012, 32(12): 3825-3833.
[16] Guo F X, Zhang M X, Chen Y, et al. Relation of several antioxidant enzymes to rapid freezing resistance in suspension cultured cells from alpine Chorispora bungeana. Cryobiology, 2006, 52: 241-250.
[17] Li B L, Mei H S.Relationship between oat leaf senescence and activated oxygen metabolism. Acta Phytophysiologica Sinica, 1989, 15(1): 6-12.
李柏林, 梅慧生. 燕麦叶片衰老与活性氧代谢的关系. 植物生理学报, 1989, 15(1): 6-12.
[18] Cui J J, Zhang X H, Li Y T, et al. Effect of silicon addition on seedling morphological and physiological indicators of Glycyrrhiza uralensis under salt stress. Acta Prataculturae Sinica, 2015, 24(10): 214-220.
崔佳佳, 张新慧, 李月彤, 等. 外源Si对盐胁迫下甘草幼苗形态及生理指标的影响. 草业学报, 2015, 24(10): 214-220.
[19] Zhang T, Liu X B, Li Z H, et al. Effects of salt stress on the growth and photosynthetic physiology characteristics of Zoysia japonica. Acta Agrestia Sinica, 2015, 23(3): 539-548.
张涛, 刘信宝, 李志华, 等. NaCl胁迫对结缕草生长及光合生理的影响. 草地学报, 2015, 23(3): 539-548.
[20] Zhao K F.Plant resistance to salt. Beijing: China Science and Technology Press, 1993.
赵可夫. 植物抗盐特性. 北京: 中国科学技术出版社, 1993.
[21] Liu A R, Zhang Y B, Zhong Z H, et al. Effects of salt stress on the growth and osmotic accumulation of Coleus blumei. Acta Prataculturae Sinica, 2013, 22(2): 211-218.
刘爱荣, 张远兵, 钟泽华, 等. 盐胁迫对彩叶草生长和渗透调节物质积累的影响. 草业学报, 2013, 22(2): 211-218.
[22] Jia X P, Deng Y M, Sun X B, et al. Impacts of salt stress on the growth and physiological characteristics of Paspalum vaginatum. Acta Prataculturae Sinica, 2015, 24(12): 204-212.
贾新平, 邓衍明, 孙晓波, 等. 盐胁迫对海滨雀稗生长和生理特性的影响. 草业学报, 2015, 24(12): 204-212.
[23] Xiong M B, Luo M S, Tian Y B, et al. Dynamics of soil nutrition and wheat root activities and their relationships during wheat growth. Soils and Fertilizers, 2005, (3): 8-11.
熊明彪, 罗茂盛, 田应兵, 等. 小麦生长期土壤养分与根系活力变化及其相关性研究. 土壤肥料, 2005, (3): 8-11.
[24] Wang S P, Guo S R, Li J, et al. Effects of salt stress on the root growth and leaf water use efficiency of cucumber seedlings. Chinese Journal of Applied Ecology, 2006, 17(10): 1883-1888.
王素平, 郭世荣, 李璟, 等. 盐胁迫对黄瓜幼苗根系生长和水分利用的影响. 应用生态学报, 2006, 17(10): 1883-1888.
[25] Chaleff R S.Further characterization of picloram-tolerant mutants of Nicotiana tabacum. Theoretical and Applied Genetics, 1980, 57(4): 91-95.
[26] Han X L.In vitro selection of L-Hydroxyproline resistant variants of Coronilla varia L. and studies on their salt tolerance. Xi’an: Northwest University, 2006.
韩晓玲. 小冠花抗L-羟基脯氨酸(Hyp)变异系离体筛选及其耐盐性研究. 西安: 西北大学, 2006.
[27] Liu A R, Zhang Y B, Fang Y Y, et al. Effects of salt stress on the growth, antioxidant ability and salt stess protein of Calendula of ficinalis. Acta Prataculturae Sinica, 2011, 20(6): 52-59.
刘爱荣, 张远兵, 方园园, 等. 盐胁迫对金盏菊生长、抗氧化能力和盐胁迫蛋白的影响. 草业学报, 2011, 20(6): 52-59.
[28] Zhu J F, Liu J T, Lu Z H, et al. Effects of salt stress on physiological characteristics of Tamarix chinensis Lour. Seedlings. Acta Ecologica Sinica, 2015, 35(15): 5140-5146.
朱金方, 刘京涛, 陆兆华, 等. 盐胁迫对中国柽柳幼苗生理特性的影响. 生态学报, 2015, 35(15): 5140-5146.
[29] Peng L X, Zhou L J, Feng T, et al. Effects of salt stress on activities of antioxidant enzymes and membrane lipid peroxidation of Elaeagnus angustifolia L. Seedlings. Journal of Tianjin Agricultural University, 2009, 16(4): 1-4.
彭立新, 周黎君, 冯涛, 等. 盐胁迫对沙枣幼苗抗氧化酶活性和膜脂过氧化的影响. 天津农学院学报, 2009, 16(4): 1-4.