乳酸菌和烷基多糖苷对玉米秸秆黄贮品质及其体外发酵特性影响研究

doi:10.11686/cyxb2018341

草业学报 ›› 2019, Vol. 28 ›› Issue (5): 109-120.DOI: 10.11686/cyxb2018341

乳酸菌和烷基多糖苷对玉米秸秆黄贮品质及其体外发酵特性影响研究

杨大盛^1,2,**, 汪水平^1,3,4,**, 韩雪峰², 汤少勋^2,*, 谭支良², 尹梦洁^1,4, 骆东梅^1,3

1.西南大学动物科学学院,重庆 402460;
2.中国科学院亚热带农业生态过程重点实验室,湖南动物安全生产协同创新中心,湖南省畜禽健康养殖工程技术中心,中国科学院亚热带农业生态研究所,湖南长沙 410125;
3.习水富兴牧业有限公司,贵州习水 556400;
4.白玉玉牧丰源畜牧科技有限公司,四川白玉 627150

收稿日期:2018-05-23 修回日期:2018-08-27 出版日期:2019-05-20 发布日期:2019-05-20
通讯作者: E-mail: wangshuiping1979@sina.com, shaoxuntang@163.com
作者简介:杨大盛(1992-),男,重庆璧山人,在读硕士。E-mail: 1134098440@qq.com;汪水平(1979-),男,湖北浠水人,副教授,博士。E-mail: wangshuiping1979@sina.com。
**共同第一作者
基金资助:
中国科学院院地合作项目(2016SYHZ0022),湖南省科技重大专项(2017NK1020)和四川省政府采购项目(5133312018000053)资助

Effects of lactic acid bacteria and alkyl polyglycoside on yellow corn stover silage quality and fermentation in vitro

YANG Da-sheng^1,2,**, WANG Shui-ping^1,3,4,**, HAN Xue-feng², TANG Shao-xun^2,*, TAN Zhi-liang², YIN Meng-jie^1,4, LUO Dong-mei^1,3

1.College of Animal Science, Southwest University, Chongqing 402460, China;
2.Key Laboratory of Subtropical Agro-ecological Processes, Chinese Academy of Sciences, Hunan Collaborative Innovation Center for Animal Production Safety, Hunan Animal and Poultry Breeding Engineering Technology Center, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; 3.Xishui Fuxing Husbandry Co., Ltd., Xishui 556400, China;
4.Baiyu Yumu Fengyuan Animal Husbandry Science and Technology Co., Ltd., Baiyu 627150, China

Received:2018-05-23 Revised:2018-08-27 Online:2019-05-20 Published:2019-05-20
Contact: E-mail: wangshuiping1979@sina.com, shaoxuntang@163.com
About author:These authors contributed equally to this work.

摘要/Abstract

摘要： 研究玉米秸秆中添加乳酸菌和烷基多糖苷时对其黄贮品质与体外发酵特性的影响。采用3×3双因素试验设计,即乳酸菌设3个水平(0、1×10¹⁰、2×10¹⁰ CFU·kg^-1),烷基多糖苷设3个水平(0、0.10、0.20 g·kg^-1),每个处理3个重复。玉米秸秆黄贮完成后,采用体外发酵技术评定其瘤胃发酵特性。结果表明:随乳酸菌添加水平的上升,黄贮玉米秸乳酸含量、比表面积、外观总评分、酸性洗涤纤维和干物质含量显著(P<0.05)提高,氨氮/总氮显著(P<0.05)降低,体外发酵潜在最大产气量显著(P<0.05)增加、总挥发性脂肪酸浓度极显著(P<0.01)增加、产气速率显著(P<0.05)降低;随烷基多糖苷添加水平的升高,黄贮玉米秸外观总评分、酸性洗涤纤维和干物质含量、以及体外发酵后Zeta电位、电子迁移率及干物质消失率显著(P<0.05)升高;黄贮玉米秸粗蛋白质、pH值、乙酸和异丁酸含量以及体外发酵后丁酸、异丁酸、戊酸和异戊酸的摩尔百分比受乳酸菌与烷基多糖苷交互作用(P<0.05)的影响。结果表明:乳酸菌添加量为2×10¹⁰ CFU·kg^-1,烷基多糖苷添加量为0.2 g·kg^-1时,既能够保证玉米秸秆较好的黄贮品质,同时又能提高其降解特性。

关键词: 乳酸菌, 烷基多糖苷, 黄贮品质, 发酵特性, 玉米秸秆

Abstract: The aim of this experiment was to study the effect of lactic acid bacteria and alkyl polyglycoside addition on the quality of yellow corn stover silage and its' in vitro fermentation characteristics. A 3×3 factorial design, i.e. three lactic acid bacteria levels (0, 1×10¹⁰, 2×10¹⁰ CFU·kg^-1) and three alkyl polyglycoside levels (0, 0.10, 0.20 g·kg^-1) with three replicates per treatment was employed in this study. After the yellow silage from corn stover had completed fermentation, its rumen degradation characteristics were evaluated by in vitro fermentation techniques. It was found that the levels of lactic acid, acid detergent fiber, and dry matter in silage, specific surface area and total score of sensory evaluation significantly (P<0.05) increased, while the ratio of ammonia to total nitrogen significantly (P<0.05) decreased with increased addition of lactic acid bacteria. The potential maximum gas production (P<0.05) and the total volatile fatty acids significantly (P<0.01) increased, while the rate of gas production was significantly (P<0.05) reduced with increased lactic acid bacteria supplementation. With increased rate of alkyl polyglycoside addition, the total score and the content of acid detergent fiber and dry matter of the silage, the Zeta potential and electron mobility of microorganism in fermentation liquor and the in vitro dry matter disappearance of the silage were significantly (P<0.05) increased. The lactic acid bacteria interacted (P<0.05) with the alkyl polyglycosides with respect to crude protein content and pH value of the silage, and the proportion of acetate, isobutyrate, butyrate, valerate, and isovalerate in vitro fermentation. In conclusion, when the dosage of lactic acid bacteria was 2×10¹⁰ CFU·kg^-1 and the amount of alkyl polyglycoside was 0.20 g·kg^-1, the quality of yellow corn stover silage could be guaranteed and the degradation characteristics were be improved.

Key words: lactic acid bacteria, alkyl polyglycosides, quality of yellow silage, fermentation characteristics, corn stover

杨大盛, 汪水平, 韩雪峰, 汤少勋, 谭支良, 尹梦洁, 骆东梅. 乳酸菌和烷基多糖苷对玉米秸秆黄贮品质及其体外发酵特性影响研究[J]. 草业学报, 2019, 28(5): 109-120.

YANG Da-sheng, WANG Shui-ping, HAN Xue-feng, TANG Shao-xun, TAN Zhi-liang, YIN Meng-jie, LUO Dong-mei. Effects of lactic acid bacteria and alkyl polyglycoside on yellow corn stover silage quality and fermentation in vitro[J]. Acta Prataculturae Sinica, 2019, 28(5): 109-120.

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乳酸菌和烷基多糖苷对玉米秸秆黄贮品质及其体外发酵特性影响研究

Effects of lactic acid bacteria and alkyl polyglycoside on yellow corn stover silage quality and fermentation in vitro

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