喷施茉莉酮酸甲酯及感染内生真菌促进醉马草抗虫性的生理作用研究

doi:10.11686/cyxb2019289

草业学报 ›› 2020, Vol. 29 ›› Issue (3): 121-129.DOI: 10.11686/cyxb2019289

喷施茉莉酮酸甲酯及感染内生真菌促进醉马草抗虫性的生理作用研究

何雅丽¹, 陈振江¹, 魏学凯¹, 张海娟¹, 刘阳², 刘辉², 李春杰^1,*

1.兰州大学草地农业生态系统国家重点实验室,兰州大学农业部草牧业创新重点实验室,兰州大学草地农业教育部工程研究中心,兰州大学甘肃省西部草业技术创新中心,兰州大学草地农业科技学院,甘肃兰州730020;
2.甘肃省临夏州农业科学院,甘肃临夏 731100

收稿日期:2019-06-19 出版日期:2020-03-20 发布日期:2020-03-20
通讯作者: *E-mail: chunjie@lzu.edu.cn
作者简介:何雅丽(1996-),女,甘肃武威人,在读硕士。E-mail: Heyl2018@lzu.edu.cn
基金资助:
国家973项目(2014CB138702),长江学者和创新团队发展计划(IRT_17R50),国家自然科学基金(30070546),中央高校基本科研业务费(LZUJBKY-2019-kb10)和111引智基地(B12002)资助

Physiological aspects of insect resistance of Achnatherum inebrians after spraying with methyl jasmonate or with Epichloё gansuensis infection

HE Ya-li¹, CHEN Zhen-jiang¹, WEI Xue-kai¹, ZHANG Hai-juan¹, LIU Yang², LIU Hui², LI Chun-jie^1,*

1.State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, Western China Technology Innovation Centre for Grassland Industry, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
2.Linxia State Academy of Agricultural Sciences, Linxia 731100, China

Received:2019-06-19 Online:2020-03-20 Published:2020-03-20

摘要/Abstract

摘要： 醉马草所携带的内生真菌能促进共生体的生长和提高共生体的生物与非生物逆境抗性。在温室栽培条件[温度:(25±2) ℃;湿度:37.7%~48.3%]下,以来自甘肃榆中的醉马草种质资源,栽培E+、E-幼苗后分别喷施200和400 mg·L^-1浓度茉莉酮酸甲酯(methyl jasmonate,MJ)以模拟昆虫的取食,测定了醉马草的生理生化指标。结果发现:与对照相比,外源喷施MJ能够诱导醉马草幼苗可溶性糖含量、叶绿素含量显著(P<0.05)降低,总酚含量显著(P<0.05)增加。MJ处理对E+和E-醉马草防御能力的诱导有所不同,对于E-醉马草,MJ处理显著提高了其防御酶多酚氧化酶和苯丙氨酸解氨酶的活性;而对于E+醉马草,MJ处理显著(P<0.05)增加胰蛋白酶抑制剂的活力。表明MJ处理对内生真菌侵染的醉马草主要通过降低可溶性糖含量、增加总酚含量以及提高体内蛋白酶抑制剂活性等途径来降低宿主植物的适口性,从而抑制植食性昆虫的取食,对其抗虫性有增益效应。

关键词: 茉莉酮酸甲酯, 内生真菌, 醉马草, 抗虫性

Abstract: The grass Achnatherum inebrians forms a symbiotic relationship with Epichloё gansuensis endophyte, which can promote plant growth and improve abiotic and biotic stress tolerance. The experiment was carried out in a greenhouse on the Yuzhong campus of Lanzhou University [Temperature (25±2) ℃; Humidity: 37.7%-48.3%], using endophyte-infected (E+) and nil-endophyte (E-) seedlings of A. inebrians from the Yuzhong region of Gansu province. After planting, seedings were exposed to exogenous methyl jasmonate (MJ) at concentrations of 200 or 400 mg·L^-1 to simulate herbivore feeding, and the physiological response assessed. It was found that, compared with the control, MJ treatments significantly decreased the soluble sugar content, and chlorophyll content (P<0.05) but significantly increased total phenol content (P<0.05). Effects of MJ treatment on herbivore resistance were different between E+ and E- plants. For E- plants, MJ treatment significantly increased polyphenoloxidase activity and phenylalanine ammonia-lyase activity. For E+ plants, the activity of trypsin inhibitors was significantly increased compared with the control group (P<0.05). In conclusion, MJ treatment strengthened the defensive ability of the host plant at the cost of reduced palatability, thus inhibiting the feeding of herbivorous insects. MJ treatment did enhance A. inebrians-mediated insect resistance.

Key words: methyl jasmonate, Epichloё endophyte, drunken horse grass, insect resistance

何雅丽, 陈振江, 魏学凯, 张海娟, 刘阳, 刘辉, 李春杰. 喷施茉莉酮酸甲酯及感染内生真菌促进醉马草抗虫性的生理作用研究[J]. 草业学报, 2020, 29(3): 121-129.

HE Ya-li, CHEN Zhen-jiang, WEI Xue-kai, ZHANG Hai-juan, LIU Yang, LIU Hui, LI Chun-jie. Physiological aspects of insect resistance of Achnatherum inebrians after spraying with methyl jasmonate or with Epichloё gansuensis infection[J]. Acta Prataculturae Sinica, 2020, 29(3): 121-129.

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喷施茉莉酮酸甲酯及感染内生真菌促进醉马草抗虫性的生理作用研究

Physiological aspects of insect resistance of Achnatherum inebrians after spraying with methyl jasmonate or with Epichloё gansuensis infection

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