Pb胁迫下内生真菌侵染对德兰臭草生长及生理的影响

doi:10.11686/cyxb2019285

摘要/Abstract

摘要： 采用盆栽试验,对带菌(E+)和不带菌(E-)的德兰臭草进行不同浓度的铅(Pb)胁迫处理,探究Pb胁迫下内生真菌侵染对德兰臭草生长及生理的影响。结果表明,随着Pb浓度的升高,德兰臭草的株高呈现先增加后减少的趋势,当胁迫浓度达到1500 mg·L^-1时,德兰臭草的株高受到了显著的抑制,且根鲜重的下降幅度最大,显著低于对照82.5%(P<0.05);德兰臭草叶片中MDA含量显著增加(P<0.05),在胁迫浓度为2000 mg·L^-1时,E+植株MDA含量显著高于对照87.3%(P<0.05);德兰臭草体内的脯氨酸含量也呈现先增加后减少的趋势,当胁迫浓度达到500 mg·L^-1时,E+植株脯氨酸含量显著高于对照35.2%(P<0.05)。与不带菌植株相比,E+植株地上和地下的生物量均高于E-植株;E+德兰臭草的MDA显著低于E-(P<0.05),在Pb浓度为2000 mg·L^-1时,E+植株的MDA含量显著低于E-25.0%(P<0.05);E+植株的脯氨酸含量在Pb浓度为1500 mg·L^-1时显著高于E-植株84.1%(P<0.05)。总的来说,感染内生真菌的德兰臭草减轻了Pb对宿主的毒害,增强其对重金属Pb胁迫的生理生化适应能力。

关键词: 德兰臭草, 内生真菌, 铅胁迫, 生理特性

Abstract: The growth and physiological characteristics of Epichloё endophyte infected (E+) and endophyte free (E-) Melica transsilvanica were observed under different Pb concentrations (0, 200, 500, 1000, 1500, 2000 mg·L^-1) in a pot experiment. It was found that with increase in Pb concentration, plant height initially increased and then decreased. At the Pb concentration of 500 mg·L^-1, the proline content of E+ plants was 35.2% higher (P<0.05) than in the Controls. At Pb concentrations of 1500 mg·L^-1 and above, plant height was significantly inhibited. The root fresh weight exhibited the largest decrease, being 82.5% lower than the control (P<0.05); meanwhile, the MDA concentration in leaves increased significantly. When the Pb concentration was 2000 mg·L^-1, the MDA content of E+ plants were significantly higher than the Control (P<0.05), and the content of proline in the plant increased initially, and then decreased. Compared with M. transsilvanica plants without Epichloё endophytic fungi, the aboveground and underground biomass of E+ plants were both higher than in E- plants; the proline content of E+ plants was significantly higher than in the E- plants at the 1500 mg·L^-1 Pb concentration (P<0.05); and the MDA content of E+ plants was 25% lower than in E- plants (P<0.05), especially at the 2000 mg·L^-1 Pb concentration. In conclusion, the growth and physiological adaptability to Pb stress of M. transsilvanica infected with endophyte fungi was enhanced.

Key words: Melica transsilvanica, endophyte, Pb stress, physiological characteristics

李柯, 施宠, 何飞焱, 李昊宇. Pb胁迫下内生真菌侵染对德兰臭草生长及生理的影响[J]. 草业学报, 2020, 29(3): 112-120.

LI Ke, SHI Chong, HE Fei-yan, LI Hao-yu. Effects of endophyte infection on growth and physiological characteristics of Melica transsilvanica under Pb stress[J]. Acta Prataculturae Sinica, 2020, 29(3): 112-120.

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