混合接种摩西球囊霉和根瘤菌对紫花苜蓿耐碱能力的影响

doi:10.11686/cyxb2019509

摘要/Abstract

摘要： 紫花苜蓿是多年生优质豆科牧草,对改善生态环境和农牧作物结构调整有重要作用。然而,土地盐碱化严重影响苜蓿正常的生长发育。丛枝菌根真菌(AMF)和根瘤菌是土壤中较为常见的共生菌,可与紫花苜蓿等豆科植物共生,并能与苜蓿形成AMF-苜蓿-根瘤菌共生系统。本研究利用摩西球囊霉菌和中华根瘤菌单接种、混合接种紫花苜蓿,比较单接种和混接种对其耐碱能力的影响。结果表明:在正常情况下,单接种和混合接种与未接种(NI)相比生长量均有所提高,主要表现在叶绿素含量更高,植株的根长、株高相对较大,分枝数较多,生长更加茂密。碱胁迫处理后,各组植株生长均受到不同程度的抑制,而混合接菌组则表现出更高的耐碱性,与NI组相比,主要表现在有较低的MDA含量和相对电导率,有较高的细胞相对含水量, 并且表现出较高的抗氧化酶的活性(SOD、POD、CAT)。综合以上指标分析,单接种和混合接种均能提高植株的渗透调节能力和抗氧化能力,降低细胞膜的损伤,但混合接种的效果更佳。

关键词: 丛枝菌根真菌, 根瘤菌, 紫花苜蓿, 耐碱性, 摩西球囊霉菌

Abstract: Alfalfa is a perennial legume with high forage yield and quality that plays an important role in eco-environment improvement and cropping rotations. However, land salinization seriously inhibits the normal growth and development of alfalfa. Arbuscular mycorrhizal fungi (AMF) and rhizobia (R) are common symbiotic soil microorganisms. They can coexist with legumes such as alfalfa, and form a symbiotic system comprising AMF-alfalfa-R. In this study, we inoculated alfalfa with Glomus mosseae and Sinorhizobium meliloti, and compared the alkali resistance of plants with inoculum combinations. It was found that without alkali stress, the growth of G. mosseae-inoculated plants or R-inoculated plants was higher than that of non-inoculated (NI) ones. Important differences included higher chlorophyll content, relatively longer root length, higher plant height, and increased branch number. With alkali stress treatment, the growth of each group was inhibited in different ways, but the inoculated groups again showed higher alkali tolerance than NI. Compared with NI, the malondialdehyde content and relative conductivity remained at a low level in the NI group. The relative water content of the cells was higher, as were the activities of superoxide dismutase, peroxidase and catalase. Based on these results, single inoculation and mixed inoculation can both improve the osmotic adjustment ability and antioxidant capacity of alfalfa, and reduce damage to the cell membrane, but the results of mixed inoculation is even better.

Key words: AMF, rhizobia, alfalfa, alkali tolerance, Glomus mosseae

邢易梅, 蕫理, 战力峰, 才华, 杨圣秋, 孙娜. 混合接种摩西球囊霉和根瘤菌对紫花苜蓿耐碱能力的影响[J]. 草业学报, 2020, 29(9): 136-145.

XING Yi-mei, DONG Li, ZHAN Li-feng, CAI Hua, YANG Sheng-qiu, SUN Na. Effect of mixed inoculation of Glomus mosseae and Sinorhizobium melilotion alkali resistance of alfalfa[J]. Acta Prataculturae Sinica, 2020, 29(9): 136-145.

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