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Acta Prataculturae Sinica ›› 2014, Vol. 23 ›› Issue (5): 256-262.DOI: 10.11686/cyxb20140530

• Orginal Article • Previous Articles     Next Articles

Isolation and identification of lucerne seed-borne growth promoting Paenibacillus polymyxa in Gansu Province, China

ZHANG Zhen-fen,NAN Zhi-biao   

  1. State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
  • Received:2012-06-14 Online:2014-10-20 Published:2014-10-20

Abstract:

Thirty nine seed samples from 13 lucerne growing areas of Gansu Province were collected and isolated for seed-borne bacteria. According to phenotypic characteristics and Biolog identification, HTBRC1, HTBRC2, HTBRC3, HTBRC4, HTBRC5, HTBRC6, HTBRC7 and HTBRC8 were preliminarily identified as Paenibacillus polymyxa. A growth promotion function following soaking with strains HTBRC1 was found in a lucerne seed germination experiment. This experiment ascertained the distribution of lucerne seed-borne P. polymyxa in different production regions of Gansu Province and discovered P. polymyxa isolated from the epidermis and internally from lucerne seed with high salt tolerance.

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