Relationship between organic acids secreted from rhizosphere phosphate?solubilizing bacteria in Trifolium pratense and phosphate?solubilizing ability

doi:10.11686/cyxb2018066

Abstract

Abstract: This study represents a preliminary exploration of the relationship between organic acid secretion and the phosphate?dissolving ability of rhizosphere phosphate?solubilizing bacteria. Four strains of excellent phosphate?solubilizing bacteria were selected from Trifolium pratense and cultured in improved PKOC2 liquid medium. The pH, phosphate?solubilizing ability, and the types and quantities of organic acids secreted were dynamically monitored by molybdenum blue colorimetry and high performance liquid chromatography (HPLC). The results showed that the strains MHS7, MHS27, MHS30, and MHS49 all secreted lactic acid, oxalic acid, malic acid, fumaric acid, and succinic acid, and strain MHS30 also secreted tartaric acid. In strain MHS7, the phosphate?solubilizing ability was negatively correlated with pH (P>0.05). In strain MHS27, the phosphate?solubilizing ability was positively correlated with pH (P>0.05) and lactic acid content (P<0.05). In strain MHS30, the phosphate?solubilizing ability was negatively correlated with pH (P<0.05), and positively correlated with the amount of organic acids secreted (P<0.01) and the tartaric acid content (P<0.05). In strain MHS49, the phosphate?solubilizing ability was significantly positively correlated with the succinic acid content (P<0.05). The types and quantities of organic acids secreted differed among the four bacterial strains. The phosphate?solubilizing ability of phosphate?solubilizing bacteria was correlated with the total organic acids content, and was also influenced by the types and quantities of organic acids released. However, the effective increase in effective phosphorus content was not determined by the total organic acids content. There were diverse relationships between the phosphate?solubilizing ability of different bacterial strains and the types and amounts of organic acids they released. These findings suggested that different phosphorus?solubilizing pathways exist in different strains of phosphate?solubilizing bacteria.

Key words: phosphate?solubilizing bacteria, phosphate?solubilizing ability, organic acid, correlation

LI Hai?yun, YAO Tuo, ZHANG Rong, ZHANG Jie, LI Zhi?yan, RONG Liang?yan, LU Xiao?wen, YANG Xiao?lei, XIA Dong?hui, LUO Hui?qin. Relationship between organic acids secreted from rhizosphere phosphate?solubilizing bacteria in Trifolium pratense and phosphate?solubilizing ability[J]. Acta Prataculturae Sinica, 2018, 27(12): 113-121.

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