Effects of lactic acid bacteria and alkyl polyglycoside on yellow corn stover silage quality and fermentation in vitro

doi:10.11686/cyxb2018341

Abstract

Abstract: The aim of this experiment was to study the effect of lactic acid bacteria and alkyl polyglycoside addition on the quality of yellow corn stover silage and its' in vitro fermentation characteristics. A 3×3 factorial design, i.e. three lactic acid bacteria levels (0, 1×10¹⁰, 2×10¹⁰ CFU·kg^-1) and three alkyl polyglycoside levels (0, 0.10, 0.20 g·kg^-1) with three replicates per treatment was employed in this study. After the yellow silage from corn stover had completed fermentation, its rumen degradation characteristics were evaluated by in vitro fermentation techniques. It was found that the levels of lactic acid, acid detergent fiber, and dry matter in silage, specific surface area and total score of sensory evaluation significantly (P<0.05) increased, while the ratio of ammonia to total nitrogen significantly (P<0.05) decreased with increased addition of lactic acid bacteria. The potential maximum gas production (P<0.05) and the total volatile fatty acids significantly (P<0.01) increased, while the rate of gas production was significantly (P<0.05) reduced with increased lactic acid bacteria supplementation. With increased rate of alkyl polyglycoside addition, the total score and the content of acid detergent fiber and dry matter of the silage, the Zeta potential and electron mobility of microorganism in fermentation liquor and the in vitro dry matter disappearance of the silage were significantly (P<0.05) increased. The lactic acid bacteria interacted (P<0.05) with the alkyl polyglycosides with respect to crude protein content and pH value of the silage, and the proportion of acetate, isobutyrate, butyrate, valerate, and isovalerate in vitro fermentation. In conclusion, when the dosage of lactic acid bacteria was 2×10¹⁰ CFU·kg^-1 and the amount of alkyl polyglycoside was 0.20 g·kg^-1, the quality of yellow corn stover silage could be guaranteed and the degradation characteristics were be improved.

Key words: lactic acid bacteria, alkyl polyglycosides, quality of yellow silage, fermentation characteristics, corn stover

YANG Da-sheng, WANG Shui-ping, HAN Xue-feng, TANG Shao-xun, TAN Zhi-liang, YIN Meng-jie, LUO Dong-mei. Effects of lactic acid bacteria and alkyl polyglycoside on yellow corn stover silage quality and fermentation in vitro[J]. Acta Prataculturae Sinica, 2019, 28(5): 109-120.

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