Transcriptome analysis of Tilletia horrida at different infection time points

doi:10.11686/cyxb2019505

Abstract

Abstract: Rice kernel smut (RSB) caused by Tilletia horrida, is one of the most important rice diseases in hybrid rice growing areas worldwide. In order to classify the mechanisms of pathogenicity, transcriptome analysis of the T. horrid strain JY-521 was conducted at different times post inoculation (8, 12, 24, 48, and 72 h) early in the infection. The highest number of differentially expressed genes (DEGs) occurred at 8 h post inoculation. Based on kyoto encyclopedia of genes and genomes (KEGG) pathway analysis of the DEGs, autophagy processes and lipid degradation were key pathways for T. horrida pathogenicity. The expression patterns of carbohydrate-active enzyme genes, pathogen-host interaction genes, and secreted protein genes were also analyzed at different times during the infection, and some DEGs that may play an important role in pathogenic progress of T. horrid were found. In summary, this research provides a new foundation for future study of the infection mechanism and control of this important rice disease.

Key words: rice, kernel smut of rice, transcriptome, pathogenic mechanism

SHU Xin-yue, JIANG Bo, MA Li, ZHENG Ai-ping. Transcriptome analysis of Tilletia horrida at different infection time points[J]. Acta Prataculturae Sinica, 2020, 29(9): 190-202.

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