Acta Prataculturae Sinica ›› 2020, Vol. 29 ›› Issue (12): 180-187.DOI: 10.11686/cyxb2020158
Previous Articles Next Articles
Cheng ZONG1(), Jian ZHANG2, Tao SHAO1, Zhi-hao DONG1, Jun-feng LI1, Lu TANG2, Qi-fan RAN2, Qin-hua LIU1()
Received:
2020-04-07
Revised:
2020-05-07
Online:
2020-12-20
Published:
2020-12-28
Contact:
Qin-hua LIU
Cheng ZONG, Jian ZHANG, Tao SHAO, Zhi-hao DONG, Jun-feng LI, Lu TANG, Qi-fan RAN, Qin-hua LIU. Effects of additives on fermentation quality of alfalfa silage[J]. Acta Prataculturae Sinica, 2020, 29(12): 180-187.
项目Items | 紫花苜蓿 Alfalfa | 标准偏差 Standard deviation |
---|---|---|
干物质 Dry matter (g·kg-1 FW) | 213.64 | 9.61 |
粗蛋白Crude protein (g·kg-1 DM) | 198.30 | 0.79 |
中性洗涤纤维Neutral detergent fiber (g·kg-1 DM) | 358.65 | 10.02 |
酸性洗涤纤维Acid detergent fiber (g·kg-1 DM) | 224.62 | 6.42 |
水溶性碳水化合物Water-soluble carbohydrates (g·kg-1 DM) | 72.60 | 3.62 |
pH | 6.01 | 0.01 |
缓冲能 Buffer capacity (mEq·kg-1 DM) | 250.13 | 16.73 |
乳酸菌Lactic acid bacteria (lg cfu·g-1 FW) | 6.70 | 0.09 |
好氧性细菌Aerobic bacteria (lg cfu·g-1 FW) | 5.35 | 0.19 |
酵母Yeasts (lg cfu·g-1 FW) | 3.49 | 0.24 |
霉菌 Mold (lg cfu·g-1 FW) | <2.00 | - |
Table 1 Chemical and microbial characteristics of alfalfa before silage
项目Items | 紫花苜蓿 Alfalfa | 标准偏差 Standard deviation |
---|---|---|
干物质 Dry matter (g·kg-1 FW) | 213.64 | 9.61 |
粗蛋白Crude protein (g·kg-1 DM) | 198.30 | 0.79 |
中性洗涤纤维Neutral detergent fiber (g·kg-1 DM) | 358.65 | 10.02 |
酸性洗涤纤维Acid detergent fiber (g·kg-1 DM) | 224.62 | 6.42 |
水溶性碳水化合物Water-soluble carbohydrates (g·kg-1 DM) | 72.60 | 3.62 |
pH | 6.01 | 0.01 |
缓冲能 Buffer capacity (mEq·kg-1 DM) | 250.13 | 16.73 |
乳酸菌Lactic acid bacteria (lg cfu·g-1 FW) | 6.70 | 0.09 |
好氧性细菌Aerobic bacteria (lg cfu·g-1 FW) | 5.35 | 0.19 |
酵母Yeasts (lg cfu·g-1 FW) | 3.49 | 0.24 |
霉菌 Mold (lg cfu·g-1 FW) | <2.00 | - |
项目 Items | pH | 有机酸含量 Organic acid content (g·kg-1 DM) | 酒精 Alcohol (g·kg-1 DM) | |||
---|---|---|---|---|---|---|
乳酸 Lactic acid | 乙酸 Acetic acid | 丙酸 Propionic acid | 丁酸 Butyric acid | |||
CON | 4.32b | 54.25bc | 49.25a | 0.00b | 1.47b | 6.78a |
SB | 4.20b | 76.33a | 51.82a | 0.00b | 1.46b | 4.13bcd |
SC | 4.79a | 30.73d | 51.21a | 0.00b | 17.36a | 3.41d |
HA | 4.35b | 43.90cd | 49.42a | 0.00b | 1.96b | 5.49abc |
M | 4.17b | 57.85bc | 44.54ab | 0.00b | 0.53b | 5.54ab |
PA | 3.89c | 64.48ab | 31.83b | 63.82a | 0.00b | 3.70cd |
标准误 Standard error of means | 0.043 | 3.544 | 3.558 | 1.792 | 0.705 | 0.385 |
显著性 Significance | <0.001 | <0.001 | 0.015 | <0.001 | <0.001 | <0.001 |
Table 2 Effects of additives on pH, organic acid and alcohol of alfalfa silage
项目 Items | pH | 有机酸含量 Organic acid content (g·kg-1 DM) | 酒精 Alcohol (g·kg-1 DM) | |||
---|---|---|---|---|---|---|
乳酸 Lactic acid | 乙酸 Acetic acid | 丙酸 Propionic acid | 丁酸 Butyric acid | |||
CON | 4.32b | 54.25bc | 49.25a | 0.00b | 1.47b | 6.78a |
SB | 4.20b | 76.33a | 51.82a | 0.00b | 1.46b | 4.13bcd |
SC | 4.79a | 30.73d | 51.21a | 0.00b | 17.36a | 3.41d |
HA | 4.35b | 43.90cd | 49.42a | 0.00b | 1.96b | 5.49abc |
M | 4.17b | 57.85bc | 44.54ab | 0.00b | 0.53b | 5.54ab |
PA | 3.89c | 64.48ab | 31.83b | 63.82a | 0.00b | 3.70cd |
标准误 Standard error of means | 0.043 | 3.544 | 3.558 | 1.792 | 0.705 | 0.385 |
显著性 Significance | <0.001 | <0.001 | 0.015 | <0.001 | <0.001 | <0.001 |
项目 Items | 乳酸与乙酸比 LA/AA | 氨态氮 NH3-N (g·kg-1 N) | 微生物数量 Microorganism number (lg cfu·g-1 FW) | |||
---|---|---|---|---|---|---|
乳酸菌 Lactic acid bacteria | 好氧细菌 Aerobic bacteria | 酵母菌 Yeasts | 霉菌 Mold | |||
CON | 1.10bc | 139.87a | 10.15 | 5.31a | <2.30 | 2.50 |
SB | 1.47ab | 133.93ab | 10.31 | 3.83bc | <2.30 | <2.30 |
SC | 0.60c | 131.30ab | 9.14 | 4.42ab | <2.30 | <2.30 |
HA | 0.90c | 141.73a | 9.08 | 4.88ab | <2.30 | <2.30 |
M | 1.30b | 125.47b | 9.61 | 5.45a | <2.30 | <2.30 |
PA | 2.09a | 111.43c | 10.19 | 3.00c | <2.30 | <2.30 |
标准误Standard error of the means | 0.137 | 2.569 | 0.550 | 0.250 | - | 0.082 |
显著性Significance | <0.001 | <0.001 | 0.151 | <0.001 | - | 0.458 |
Table 3 Effects of additives on the ratio of lactic acid to acetic acid, ammonia nitrogen and microorganism of alfalfa silage
项目 Items | 乳酸与乙酸比 LA/AA | 氨态氮 NH3-N (g·kg-1 N) | 微生物数量 Microorganism number (lg cfu·g-1 FW) | |||
---|---|---|---|---|---|---|
乳酸菌 Lactic acid bacteria | 好氧细菌 Aerobic bacteria | 酵母菌 Yeasts | 霉菌 Mold | |||
CON | 1.10bc | 139.87a | 10.15 | 5.31a | <2.30 | 2.50 |
SB | 1.47ab | 133.93ab | 10.31 | 3.83bc | <2.30 | <2.30 |
SC | 0.60c | 131.30ab | 9.14 | 4.42ab | <2.30 | <2.30 |
HA | 0.90c | 141.73a | 9.08 | 4.88ab | <2.30 | <2.30 |
M | 1.30b | 125.47b | 9.61 | 5.45a | <2.30 | <2.30 |
PA | 2.09a | 111.43c | 10.19 | 3.00c | <2.30 | <2.30 |
标准误Standard error of the means | 0.137 | 2.569 | 0.550 | 0.250 | - | 0.082 |
显著性Significance | <0.001 | <0.001 | 0.151 | <0.001 | - | 0.458 |
项目Items | 干物质 Dry matter (g·kg-1 FW) | 营养品质 Nutrient quality (g·kg-1 DM) | |||
---|---|---|---|---|---|
粗蛋白 Crude protein | 中性洗涤纤维 Neutral detergent fiber | 酸性洗涤纤维 Acid detergent fiber | 水溶性碳水化合物 Water-soluble carbohydrates | ||
CON | 198.17 | 207.57ab | 389.40a | 275.67a | 17.38b |
SB | 204.03 | 202.70ab | 355.63b | 253.77b | 14.49bc |
SC | 216.47 | 207.75ab | 395.70a | 261.03b | 14.28bc |
HA | 215.40 | 200.30b | 387.20a | 252.93b | 12.98c |
M | 216.97 | 219.17ab | 322.07c | 227.17c | 23.05a |
PA | 199.53 | 221.73a | 379.87ab | 252.70b | 23.63a |
标准误Standard error of means | 4.905 | 4.274 | 5.465 | 2.958 | 0.879 |
显著性Significance | 0.144 | 0.020 | <0.001 | <0.001 | <0.001 |
Table 4 Effects of additives on nutrient quality of alfalfa silage
项目Items | 干物质 Dry matter (g·kg-1 FW) | 营养品质 Nutrient quality (g·kg-1 DM) | |||
---|---|---|---|---|---|
粗蛋白 Crude protein | 中性洗涤纤维 Neutral detergent fiber | 酸性洗涤纤维 Acid detergent fiber | 水溶性碳水化合物 Water-soluble carbohydrates | ||
CON | 198.17 | 207.57ab | 389.40a | 275.67a | 17.38b |
SB | 204.03 | 202.70ab | 355.63b | 253.77b | 14.49bc |
SC | 216.47 | 207.75ab | 395.70a | 261.03b | 14.28bc |
HA | 215.40 | 200.30b | 387.20a | 252.93b | 12.98c |
M | 216.97 | 219.17ab | 322.07c | 227.17c | 23.05a |
PA | 199.53 | 221.73a | 379.87ab | 252.70b | 23.63a |
标准误Standard error of means | 4.905 | 4.274 | 5.465 | 2.958 | 0.879 |
显著性Significance | 0.144 | 0.020 | <0.001 | <0.001 | <0.001 |
项目Items | TFA | C12:0 | C13:0 | C14:0 | C14:1 | C16:0 | C18:0 | C18:1 | C18:2n6 | C18:3n3 |
---|---|---|---|---|---|---|---|---|---|---|
RAM | 19.22cd | 0.08b | 0.34ab | 0.19a | 0.76a | 5.14ab | 0.70a | 0.64a | 3.41c | 5.83c |
CON | 22.11bc | 0.17ab | 0.33ab | 0.20a | 0.76a | 5.40ab | 0.72a | 0.62a | 4.99ab | 8.02b |
SB | 26.87a | 0.22a | 0.38a | 0.23a | 0.82a | 6.47a | 0.84a | 0.70a | 5.84a | 10.49a |
SC | 24.20ab | 0.22a | 0.35ab | 0.21a | 0.78a | 5.68ab | 0.74a | 0.58ab | 5.21ab | 9.37ab |
HA | 22.93abc | 0.20a | 0.37a | 0.21a | 0.81a | 5.90ab | 0.79a | 0.69a | 4.08bc | 8.68b |
M | 15.84d | 0.12ab | 0.24b | 0.14b | 0.51b | 3.94b | 0.52b | 0.46b | 2.88c | 5.78c |
PA | 24.48ab | 0.23a | 0.36a | 0.22a | 0.78a | 5.46ab | 0.72a | 0.60ab | 4.97ab | 9.74ab |
标准误 Standard error of means | 0.935 | 0.023 | 0.024 | 0.010 | 0.032 | 0.240 | 0.029 | 0.032 | 0.263 | 0.375 |
显著性 Significance | <0.001 | 0.004 | 0.018 | <0.001 | <0.001 | <0.001 | <0.001 | 0.002 | <0.001 | <0.001 |
Table 5 The change of fatty acids in alfalfa silage before and after ensiling (g·kg-1 DM)
项目Items | TFA | C12:0 | C13:0 | C14:0 | C14:1 | C16:0 | C18:0 | C18:1 | C18:2n6 | C18:3n3 |
---|---|---|---|---|---|---|---|---|---|---|
RAM | 19.22cd | 0.08b | 0.34ab | 0.19a | 0.76a | 5.14ab | 0.70a | 0.64a | 3.41c | 5.83c |
CON | 22.11bc | 0.17ab | 0.33ab | 0.20a | 0.76a | 5.40ab | 0.72a | 0.62a | 4.99ab | 8.02b |
SB | 26.87a | 0.22a | 0.38a | 0.23a | 0.82a | 6.47a | 0.84a | 0.70a | 5.84a | 10.49a |
SC | 24.20ab | 0.22a | 0.35ab | 0.21a | 0.78a | 5.68ab | 0.74a | 0.58ab | 5.21ab | 9.37ab |
HA | 22.93abc | 0.20a | 0.37a | 0.21a | 0.81a | 5.90ab | 0.79a | 0.69a | 4.08bc | 8.68b |
M | 15.84d | 0.12ab | 0.24b | 0.14b | 0.51b | 3.94b | 0.52b | 0.46b | 2.88c | 5.78c |
PA | 24.48ab | 0.23a | 0.36a | 0.22a | 0.78a | 5.46ab | 0.72a | 0.60ab | 4.97ab | 9.74ab |
标准误 Standard error of means | 0.935 | 0.023 | 0.024 | 0.010 | 0.032 | 0.240 | 0.029 | 0.032 | 0.263 | 0.375 |
显著性 Significance | <0.001 | 0.004 | 0.018 | <0.001 | <0.001 | <0.001 | <0.001 | 0.002 | <0.001 | <0.001 |
1 | Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects-A review. Journal of Functional Foods, 2015, 18: 820-897. |
2 | Simopoulos A P. N-3 fatty acids and human health: Defining strategies for public policy. Lipids, 2001, 36(1): S83-S89. |
3 | Eggersdorfer M, Wyss A. Carotenoids in human nutrition and health. Archives of Biochemistry and Biophysics, 2018, 652: 18-26. |
4 | Han L Y, Zhou H, Yu Z. Research progress of fatty acid in silage. Acta Agrestia Sinica, 2012, 20(6): 990-994. |
韩立英, 周禾, 玉柱. 青贮饲料中脂肪酸研究进展. 草地学报, 2012, 20(6): 990-994. | |
5 | Alves S P, Cabrita A R J, Jerónimo E, et al. Effect of ensiling and silage additives on fatty acid composition of ryegrass and corn experimental silages. Journal of Animal Science, 2011, 89(8): 2537-2545. |
6 | Williams C M. Dietary fatty acids and human health. Annales De Zootechnie, 2000, 49(3): 165-180. |
7 | Warren H E, Scollan N D, Nute G R, et al. Effects of breed and a concentrate or grass silage diet on beef quality in cattle of 3 ages. II: Meat stability and flavour. Meat Science, 2008, 78(3): 270-278. |
8 | Bernes G, Turner T, Pickova J. Sheep fed only silage or silage supplemented with concentrates: 2. Effects on lamb performance and fatty acid profile of ewe milk and lamb meat. Small Ruminant Research, 2012, 102(2/3): 114-124. |
9 | Liu Q H, Dong Z H, Shao T. Effect of additives on fatty acid profile of high moisture alfalfa silage during ensiling and after exposure to air. Animal Feed Science and Technology, 2018, 236: 29-38. |
10 | Arvidsson K, Gustavsson A M, Martinsson K. Effects of conservation method on fatty acid composition of silage. Animal Feed Science and Technology, 2009, 148(2/3/4): 241-252. |
11 | Boufaïed H, Chouinard P Y, Tremblay G F, et al. Fatty acids in forages. I. Factors affecting concentrations. Canadian Journal of Animal Science, 2003, 83(3): 501-511. |
12 | Elgersma A, Ellen G, Van der Horst H, et al. Comparison of the fatty acid composition of fresh and ensiled perennial ryegrass (Lolium perenne L.), affected by cultivar and regrowth interval. Animal Feed Science and Technology, 2003, 108(1/2/3/4): 191-205. |
13 | Kishino S, Ogawa J, Yokozeki K, et al. Metabolic diversity in biohydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production. Applied Microbiology and Biotechnology, 2009, 84(1): 87-97. |
14 | Ding W R, Long R J, Guo X S. Effects of plant enzyme inactivation or sterilization on lipolysis and proteolysis in alfalfa silage. Journal of Dairy Science, 2013, 96(4): 2536-2543. |
15 | Wang X J, Li W F, Tang C B, et al. Synergistic effect of sodium caseinate and sunflower oil on characteristics of emulsified sausages. Food Science, 2015, 36(3): 51-56. |
王晓娟, 李伟锋, 唐长波, 等. 酪蛋白酸钠-葵花籽油协同对乳化肠品质特性的影响. 食品科学, 2015, 36(3): 51-56. | |
16 | Liang Q. Study on founctional properties of sodium caseinate. Food Science, 2002(3): 30-33. |
梁琪. 酪蛋白酸钠功能性的研究. 食品科学, 2002(3): 30-33. | |
17 | Liu Q H, Lindow S E, Zhang J G. Lactobacillus parafarraginis ZH1 producing anti-yeast substances to improve the aerobic stability of silage. Animal Science Journal, 2018, 89(9): 1302-1309. |
18 | Liu Q H, Wu J X, Shao T. Roles of microbes and lipolytic enzymes in changing the fatty acid profile, α-tocopherol and β-carotene of whole-crop oat silages during ensiling and after exposure to air. Animal Feed Science and Technology, 2019, 253: 81-92. |
19 | Chen X Z, Gao C F, Zhang X P, et al. Effects of molasses on the fermentation quality of mixed silage of ramie and hybrid pennisetum. Acta Agrestia Sinica, 2016, 24(6): 1358-1362. |
陈鑫珠, 高承芳, 张晓佩, 等. 糖蜜对不同比例苎麻杂交狼尾草混合青贮发酵品质的影响. 草地学报, 2016, 24(6): 1358-1362. | |
20 | Liu Q H, Li X Y, Li J F, et al. Effect of temperature and additives on fermentation and α-tocopherol and β-carotene content of Pennisetum purpureum silage. Acta Prataculturae Sinica, 2015, 24(7): 116-122. |
刘秦华, 李湘玉, 李君风, 等. 温度和添加剂对象草青贮发酵品质、α-生育酚和β-胡萝卜素的影响. 草业学报, 2015, 24(7): 116-122. | |
21 | Zhao Q J, Yuan X J, Guo G, et al. Effect of adding an inoculant and molasses on fermentation quality of mixed silage of hull-lessbarley straw and perennial ryegrass in Tibet. Acta Prataculturae Sinica, 2014, 23(4): 100-106. |
赵庆杰, 原现军, 郭刚, 等. 添加糖蜜和乳酸菌制剂对西藏青稞秸秆和多年生黑麦草混合青贮发酵品质的影响. 草业学报, 2014, 23(4): 100-106. | |
22 | Yan Y H, Li J L, Guo X S, et al. A study on fermentation quality of Italian ryegrass and soybean straw mixed silage. Acta Prataculturae Sinica, 2014, 23(4): 94-99. |
闫艳红, 李君临, 郭旭生, 等. 多花黑麦草与大豆秸秆混合青贮发酵品质的研究.草业学报, 2014, 23(4): 94-99. | |
23 | Wang C, Nishino N. Effects of storage temperature and ensiling period on fermentation products, aerobic stability and microbial communities of total mixed ration silage. Journal of Applied Microbiology, 2013, 114(6): 1687-1695. |
24 | Liu Q H, Zhang J G, Lu X L. The effects of lactic acid bacteria inoculation on the fermentation quality and aerobic stability of king grass silage. Acta Prataculturae Sinica, 2009, 18(4): 131-137. |
刘秦华, 张建国, 卢小良. 乳酸菌添加剂对王草青贮发酵品质及有氧稳定性的影响. 草业学报, 2009, 18(4): 131-137. | |
25 | Wang X N, Sun Q Z, Han H B, et al. The quality of silage in Inner Mongolia. Acta Prataculturae Sinica, 2011, 20(3): 149-155. |
王晓娜, 孙启忠, 韩海波, 等. 内蒙古青贮饲料质量研究. 草业学报, 2011, 20(3): 149-155. | |
26 | Liu Q H, Wu J X, Dong Z H, et al. Effects of overnight wilting and additives on the fatty acid profile, α-tocopherol and β-carotene of whole plant oat silages. Animal Feed Science and Technology, 2020, 260: DOI: 10.1016/j.anifeedsci.2019.114370. |
27 | Ennahar S, Cai Y, Fujita Y. Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis. Applied and Environmental Microbiology, 2003, 69(1): 444-451. |
28 | Wang J, Wang J Q, Zhou H, et al. Effects of addition of previously fermented juice prepared from alfalfa on fermentation quality and protein degradation of alfalfa silage. Animal Feed Science and Technology, 2009, 151(3): 280-290. |
29 | Zhang Z X, Shao T. The effect of propionic acid addition on the dynamic fermentation changes of Italian ryegrass (Lolium multiflorum) silage. Acta Prataculturae Sinica, 2009, 18(2): 102-107. |
张增欣, 邵涛. 丙酸对多花黑麦草青贮发酵动态变化的影响. 草业学报, 2009, 18(2): 102-107. | |
30 | Kung Jr L, Myers C L, Neylon J M, et al. The effects of buffered propionic acid-based additives alone or combined with microbial inoculation on the fermentation of high moisture corn and whole-crop barley. Journal of Dairy Science, 2004, 87(5): 1310-1316. |
31 | Kung Jr L, Ranjit N K. The effect of lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. Journal of Dairy Science, 2001, 84(5): 1149-1155. |
32 | Wang J, Wang Y Q, Wen A Y, et al. Effects of molasses addition on the fermentation quality of broccoli residue, rice straw and alfalfa mixed silage. Acta Prataculturae Sinica, 2014, 23(3): 248-254. |
王坚, 王亚琴, 闻爱友, 等. 糖蜜添加对西兰花茎叶稻秸苜蓿混合青贮发酵品质的影响. 草业学报, 2014, 23(3): 248-254. | |
33 | Ke W C, Yang F Y, Undersander D J, et al. Fermentation characteristics, aerobic stability, proteolysis and lipid composition of alfalfa silage ensiled with apple or grape pomace. Animal Feed Science and Technology, 2015, 202: 12-19. |
34 | Van R G, Fievez V, Vandewalle M, et al. Influence of herbage species, cultivar and cutting date on fatty acid composition of herbage and lipid metabolism during ensiling. Grass and Forage Science, 2009, 64(2): 196-207. |
35 | Luna C M, Trippi V S. Membrane permeability-regulation by exogenous sugars during senescence of oat leaf in light and darkness. Plant and Cell Physiology, 1986, 27: 1051-1061. |
36 | Heimann E, Nyman M, Degerman E. Propionic acid and butyric acid inhibit lipolysis and de novo lipogenesis and increase insulin-stimulated glucose uptake in primary rat adipocytes. Adipocyte, 2015, 4(2): 81-88. |
[1] | Fa-ming PAN, Sheng-hua CHANG, Guo-dong WANG, Sheng-yan HAO, Jia LIU, Hui-yuan ZHANG, Yin-ping XU. Effects of phenological period on the composition of fatty acids and conjugated linoleic acids in rumen fluid, forage and milk fat of grazing yak and their correlation analysis [J]. Acta Prataculturae Sinica, 2021, 30(3): 110-120. |
[2] | Sheng-wei ZHANG, Xiao-ping WANG, Zhan-hai ZHANG, You-ji MA, Shuang-bao GUN, Qiao-li YANG, Xiao-li GAO, Bao-jun ZHANG. Effects of Broussonetia papyrifera silage on growth performance, serum biochemical indexes and meat quality of Dorper×Hu crossbred sheep [J]. Acta Prataculturae Sinica, 2021, 30(3): 89-99. |
[3] | Ji-qing WANG, Ji-yuan SHEN, Xiu LIU, Shao-bin LI, Yu-zhu LUO, Meng-li ZHAO, Zhi-yun HAO, Na KE, Yi-ze SONG, Li-rong QIAO. Comparative analysis of meat production traits, meat quality, and muscle nutrient and fatty acid contents between Ziwuling black goats and Liaoning cashmere goats [J]. Acta Prataculturae Sinica, 2021, 30(2): 166-177. |
[4] | WANG Yu-ping, GAO Chun-xiao, WANG Sheng-xiang, HE Xiao-tong. Changes in photoinhibition and fatty acid composition in the thylakoid membrane of kidney bean leaves under low temperature and weak light stress [J]. Acta Prataculturae Sinica, 2020, 29(8): 116-125. |
[5] | DONG Wen-cheng, LIN Yu-fan, ZHU Hong-fu, ZHANG Huan, ZHANG Gui-jie. Effects of different grape variety on proteolysis and aerobic stability of alfalfa silage made with added grape pomace [J]. Acta Prataculturae Sinica, 2020, 29(4): 129-137. |
[6] | YU Hao-ran, GE Gen-tu, WANG Zhi-jun, JIA Yu-shan, LIAN Zhi, JIA Peng-fei. Effects of formic acid additives and ensiling time on the quality of alfalfa silage [J]. Acta Prataculturae Sinica, 2020, 29(3): 89-95. |
[7] | REN Yu-xin, DAI Han-ling, TIAN Xin-hui, DU Wen-hua. Effects of different additives and cutting dates on nutritional and silage fermentation quality of Triticale silage in an alpine pastoral area of Gansu Province [J]. Acta Prataculturae Sinica, 2020, 29(3): 197-206. |
[8] | LI Yan-fen, CHENG Jin-hua, TIAN Chuan-yao, TIAN Yu-jia, LU Dong-ya, ZHANG Jian-bin. Effects of sodium diacetate on the quality, nutrient composition and protein molecular structure of alfalfa silage [J]. Acta Prataculturae Sinica, 2020, 29(2): 163-171. |
[9] | MAO Cui, LIU Fang-yuan, SONG En-liang, WANG Ya-fang, WANG Yong-jun, ZHAN Xiang, LI Yuan, CHENG Hai-jian, JIANG Fu-gui. Effects of lactic acid bacteria inoculant level and ensiling time on nutritional value and fermentation quality of whole-crop maize silage [J]. Acta Prataculturae Sinica, 2020, 29(10): 172-181. |
[10] | ZHANG Xiang, YANG Yong, LIU Xue-yong, XIANG Zuo-xiang. Effect of exogenous salicylic acid on the antioxidant enzyme activities and fatty acid profiles in seashore paspalum under low temperature stress [J]. Acta Prataculturae Sinica, 2020, 29(1): 117-124. |
[11] | JU Ze-liang, ZHAO Gui-qin, CHAI Ji-kuan, JIA Zhi-feng, LIANG Guo-ling. Comprehensive evaluation of nutritional value and silage fermentation quality of different oat varieties in central Gansu Province [J]. Acta Prataculturae Sinica, 2019, 28(9): 77-86. |
[12] | ZHANG Xia, LI Miao-shan, ZHOU En-guang, WANG Hu-cheng. An in vitro study of fermentation properties of four high-quality forages and their mixtures treated with rumen fluid from beef cattle in northwest China [J]. Acta Prataculturae Sinica, 2019, 28(9): 135-145. |
[13] | LI Xiao-ling, GUAN Hao, SHUAI Yang, LI Xiao-mei, PENG An-qi, LI Chang-hua, PU Qi, YAN Yan-hong, ZHANG Xin-quan. Effects of single and multiple inoculants on Hemarthria compressa silage quality [J]. Acta Prataculturae Sinica, 2019, 28(6): 119-127. |
[14] | LIU Yue, WANG Guo-gen, WU Hao, MENG Qing-xiang, SONG En-liang, CHENG Hai-jian, ZHOU Zhen-ming. Variety effects on fermentation quality and nutritive value of whole-plant maize silage [J]. Acta Prataculturae Sinica, 2019, 28(6): 148-156. |
[15] | LUO Ying-jie, CHEN Gui-hua, MU Lin, HU Long-xing, ZHANG Zhi-fei, GAO Shuai, WEI Zhong-shan. Effects on silage quality of mixing different ratios of rice straw with alfalfa and wheat bran [J]. Acta Prataculturae Sinica, 2019, 28(5): 178-184. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||