Acta Prataculturae Sinica ›› 2021, Vol. 30 ›› Issue (6): 141-150.DOI: 10.11686/cyxb2020323
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Wang-pan QI1,2(), Ying-yu MU1,2, Tao ZHANG1,2, Ji-you ZHANG1,2, Sheng-yong MAO1,2()
Received:
2020-07-07
Revised:
2020-11-02
Online:
2021-05-21
Published:
2021-05-21
Contact:
Sheng-yong MAO
Wang-pan QI, Ying-yu MU, Tao ZHANG, Ji-you ZHANG, Sheng-yong MAO. Plasma biochemical indexes and metabolomics profile changes of dairy cows with subacute ruminal acidosis[J]. Acta Prataculturae Sinica, 2021, 30(6): 141-150.
项目 Item | 对照组 CON | 处理组 SARA |
---|---|---|
日粮组成 Diet ingredient | ||
苜蓿草 Alfalfa (%DM) | 24.00 | 17.00 |
燕麦草 Oat grass (%DM) | 24.00 | 17.00 |
青贮玉米 Silage (%DM) | 12.00 | 6.00 |
玉米 Corn (%DM) | 19.40 | 24.92 |
豆粕 Soybean meal (%DM) | 13.50 | 13.48 |
大麦 Barley (%DM) | 0.00 | 12.00 |
玉米酒精糟 Corn distiller’s grains (%DM) | 3.80 | 5.91 |
石粉 Limestone (%DM) | 0.80 | 1.48 |
磷酸氢钙 Calcium monohydrogen phosphate (%DM) | 1.10 | 0.92 |
食盐 Salt (%DM) | 0.40 | 0.37 |
预混料 Premix1 (%DM) | 1.00 | 0.92 |
营养水平 Nutritional level | ||
粗蛋白 Crude protein (%DM) | 16.16 | 16.12 |
钙 Ca (%DM) | 1.14 | 1.18 |
磷 P (%DM) | 0.52 | 0.51 |
中性洗涤纤维 Neutral detergent fiber (%DM) | 36.14 | 29.92 |
非纤维性碳水化合物 Non-fibrous carbohydrate (%DM) | 35.39 | 42.34 |
粗灰分 Crude ash (%DM) | 5.97 | 4.87 |
泌乳净能 Net energy (Mcal·kg-1) | 1.57 | 1.64 |
粗脂肪 Ether extracts (%DM) | 3.05 | 3.05 |
淀粉 Starch (%DM) | 17.96 | 27.82 |
Table 1 Ingredient and nutrient composition of diet
项目 Item | 对照组 CON | 处理组 SARA |
---|---|---|
日粮组成 Diet ingredient | ||
苜蓿草 Alfalfa (%DM) | 24.00 | 17.00 |
燕麦草 Oat grass (%DM) | 24.00 | 17.00 |
青贮玉米 Silage (%DM) | 12.00 | 6.00 |
玉米 Corn (%DM) | 19.40 | 24.92 |
豆粕 Soybean meal (%DM) | 13.50 | 13.48 |
大麦 Barley (%DM) | 0.00 | 12.00 |
玉米酒精糟 Corn distiller’s grains (%DM) | 3.80 | 5.91 |
石粉 Limestone (%DM) | 0.80 | 1.48 |
磷酸氢钙 Calcium monohydrogen phosphate (%DM) | 1.10 | 0.92 |
食盐 Salt (%DM) | 0.40 | 0.37 |
预混料 Premix1 (%DM) | 1.00 | 0.92 |
营养水平 Nutritional level | ||
粗蛋白 Crude protein (%DM) | 16.16 | 16.12 |
钙 Ca (%DM) | 1.14 | 1.18 |
磷 P (%DM) | 0.52 | 0.51 |
中性洗涤纤维 Neutral detergent fiber (%DM) | 36.14 | 29.92 |
非纤维性碳水化合物 Non-fibrous carbohydrate (%DM) | 35.39 | 42.34 |
粗灰分 Crude ash (%DM) | 5.97 | 4.87 |
泌乳净能 Net energy (Mcal·kg-1) | 1.57 | 1.64 |
粗脂肪 Ether extracts (%DM) | 3.05 | 3.05 |
淀粉 Starch (%DM) | 17.96 | 27.82 |
时间 Time (min) | 流速 Flow rate (mL·min-1) | 流动相A Mobile phase A (%) | 流动相B Mobile phase B (%) |
---|---|---|---|
0 | 0.3 | 95 | 5 |
1 | 0.3 | 95 | 5 |
2 | 0.3 | 60 | 40 |
7 | 0.3 | 20 | 80 |
11 | 0.3 | 5 | 95 |
15.5 | 0.3 | 95 | 5 |
19.5 | 0.3 | 95 | 5 |
Table 2 Mobile phase elution procedures
时间 Time (min) | 流速 Flow rate (mL·min-1) | 流动相A Mobile phase A (%) | 流动相B Mobile phase B (%) |
---|---|---|---|
0 | 0.3 | 95 | 5 |
1 | 0.3 | 95 | 5 |
2 | 0.3 | 60 | 40 |
7 | 0.3 | 20 | 80 |
11 | 0.3 | 5 | 95 |
15.5 | 0.3 | 95 | 5 |
19.5 | 0.3 | 95 | 5 |
项目 Item | 对照组 CON (mmol·L-1) | 试验组 SARA (mmol·L-1) | 标准误 SEM | P值 P-value | ||
---|---|---|---|---|---|---|
饲喂天数 Feeding days | 日粮 Diet | 饲喂天数*日粮 Day*diet | ||||
β-羟丁酸 β-hydroxybutyric acid (BHBA) | 0.449 | 0.393 | 0.013 | 0.200 | 0.007 | 0.002 |
葡萄糖 Glucose (Glu) | 6.166 | 6.894 | 0.224 | 0.003 | 0.057 | 0.867 |
胆固醇 Cholesterol (TC) | 5.323 | 5.151 | 0.171 | 0.691 | 0.645 | 0.783 |
甘油三酯 Triglyceride (TG) | 1.228 | 1.508 | 0.061 | 0.064 | 0.014 | 0.499 |
游离脂肪酸Nonestesterified fatty acid (NEFA) | 0.153 | 0.148 | 0.004 | 0.284 | 0.584 | 0.865 |
Table 3 Effects of SARA on plasma biochemical indexes
项目 Item | 对照组 CON (mmol·L-1) | 试验组 SARA (mmol·L-1) | 标准误 SEM | P值 P-value | ||
---|---|---|---|---|---|---|
饲喂天数 Feeding days | 日粮 Diet | 饲喂天数*日粮 Day*diet | ||||
β-羟丁酸 β-hydroxybutyric acid (BHBA) | 0.449 | 0.393 | 0.013 | 0.200 | 0.007 | 0.002 |
葡萄糖 Glucose (Glu) | 6.166 | 6.894 | 0.224 | 0.003 | 0.057 | 0.867 |
胆固醇 Cholesterol (TC) | 5.323 | 5.151 | 0.171 | 0.691 | 0.645 | 0.783 |
甘油三酯 Triglyceride (TG) | 1.228 | 1.508 | 0.061 | 0.064 | 0.014 | 0.499 |
游离脂肪酸Nonestesterified fatty acid (NEFA) | 0.153 | 0.148 | 0.004 | 0.284 | 0.584 | 0.865 |
差异代谢物 Differential metabolites | 贡献度 Contribution (VIP) | P 值 P-value (FDR) | 倍数关系Multiple relations (FC, SARA∶CON) |
---|---|---|---|
脂质及脂肪酸 Lipids and fatty acids | |||
7-酮脱氧胆酸 7-ketodeoxycholic acid | 1.743 | 0.010 | 4.557 |
脱氧胆酸Deoxycholic acid | 1.518 | 0.021 | 3.271 |
胆酸Cholic acid | 1.635 | 0.006 | 2.758 |
12-酮脱氧胆酸12-Ketodeoxycholic acid | 1.944 | 0.012 | 2.706 |
12(13)Ep-9-KODE | 2.494 | 0.006 | 1.475 |
12,13-DHOME | 1.753 | 0.007 | 1.403 |
十一烷二酸Undecanedioic acid | 1.636 | 0.025 | 0.771 |
十六烷二酸Hexadecanedioic acid | 1.816 | 0.006 | 0.623 |
9-HODE | 2.331 | 0.001 | 0.491 |
血氧烷B3 Thromboxane B3 | 1.963 | 0.001 | 0.373 |
前列腺素E2 Prostaglandin E2 | 2.062 | 0.001 | 0.330 |
氨基酸类及其衍生物 Amino acids and derivatives | |||
L-天冬酰胺L-Asparagine | 1.035 | 0.012 | 1.832 |
L-精氨酸L-Arginine | 1.626 | 0.045 | 0.816 |
L-色氨酸L-Tryptophan | 1.482 | 0.018 | 0.792 |
L-苏氨酸L-Threonine | 1.716 | 0.045 | 0.763 |
异丁酰甘氨酸Isobutyrylglycine | 2.022 | 0.001 | 0.645 |
异戊酰甘氨酸Isovalerylglycine | 1.558 | 0.039 | 0.628 |
有机酸 Organic acids | |||
3-羟基十二烷二酸3-Hydroxydodecanedioic acid | 1.630 | 0.018 | 0.719 |
酮亮氨酸Ketoleucine | 1.767 | 0.025 | 0.709 |
苯甲酸及其衍生物 Benzoic acids and derivatives | |||
马尿酸Hippuric acid | 2.152 | 0.006 | 0.728 |
4-羟基马尿酸4-Hydroxyhippuric acid | 1.958 | 0.005 | 0.592 |
其他 Others | |||
6-磷酸-2-脱氢-D-葡萄糖酸酯6-phospho-2-dehydro-D-gluconate | 2.319 | 0.045 | 0.811 |
硬脂酰乙醇酰胺Stearoylethanolamide | 1.527 | 0.039 | 0.781 |
吡哆胺Pyridoxamine | 1.978 | 0.045 | 0.748 |
吲哚Indole | 2.257 | 0.006 | 0.726 |
吲哚乳酸Indolelactic acid | 1.731 | 0.039 | 0.560 |
Table 4 Plasma differential metabolites
差异代谢物 Differential metabolites | 贡献度 Contribution (VIP) | P 值 P-value (FDR) | 倍数关系Multiple relations (FC, SARA∶CON) |
---|---|---|---|
脂质及脂肪酸 Lipids and fatty acids | |||
7-酮脱氧胆酸 7-ketodeoxycholic acid | 1.743 | 0.010 | 4.557 |
脱氧胆酸Deoxycholic acid | 1.518 | 0.021 | 3.271 |
胆酸Cholic acid | 1.635 | 0.006 | 2.758 |
12-酮脱氧胆酸12-Ketodeoxycholic acid | 1.944 | 0.012 | 2.706 |
12(13)Ep-9-KODE | 2.494 | 0.006 | 1.475 |
12,13-DHOME | 1.753 | 0.007 | 1.403 |
十一烷二酸Undecanedioic acid | 1.636 | 0.025 | 0.771 |
十六烷二酸Hexadecanedioic acid | 1.816 | 0.006 | 0.623 |
9-HODE | 2.331 | 0.001 | 0.491 |
血氧烷B3 Thromboxane B3 | 1.963 | 0.001 | 0.373 |
前列腺素E2 Prostaglandin E2 | 2.062 | 0.001 | 0.330 |
氨基酸类及其衍生物 Amino acids and derivatives | |||
L-天冬酰胺L-Asparagine | 1.035 | 0.012 | 1.832 |
L-精氨酸L-Arginine | 1.626 | 0.045 | 0.816 |
L-色氨酸L-Tryptophan | 1.482 | 0.018 | 0.792 |
L-苏氨酸L-Threonine | 1.716 | 0.045 | 0.763 |
异丁酰甘氨酸Isobutyrylglycine | 2.022 | 0.001 | 0.645 |
异戊酰甘氨酸Isovalerylglycine | 1.558 | 0.039 | 0.628 |
有机酸 Organic acids | |||
3-羟基十二烷二酸3-Hydroxydodecanedioic acid | 1.630 | 0.018 | 0.719 |
酮亮氨酸Ketoleucine | 1.767 | 0.025 | 0.709 |
苯甲酸及其衍生物 Benzoic acids and derivatives | |||
马尿酸Hippuric acid | 2.152 | 0.006 | 0.728 |
4-羟基马尿酸4-Hydroxyhippuric acid | 1.958 | 0.005 | 0.592 |
其他 Others | |||
6-磷酸-2-脱氢-D-葡萄糖酸酯6-phospho-2-dehydro-D-gluconate | 2.319 | 0.045 | 0.811 |
硬脂酰乙醇酰胺Stearoylethanolamide | 1.527 | 0.039 | 0.781 |
吡哆胺Pyridoxamine | 1.978 | 0.045 | 0.748 |
吲哚Indole | 2.257 | 0.006 | 0.726 |
吲哚乳酸Indolelactic acid | 1.731 | 0.039 | 0.560 |
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