Analysis of differentially expressed protein kinase related genes in the xerophyte Pugionium cornutum under salt treatment

doi:10.11686/cyxb2020368

Abstract

Abstract:

The xerophyte Pugionium cornutum possesses strong salt tolerance. Study of the molecular basis of its salt tolerance will provide important genetic resources for the genetic improvement of stress tolerance in crops and forages. In previous studies， by using transcriptomic analysis， the expression pattern of important functional genes in P. cornutum under salt stress has been analyzed， and a number of candidate functional genes related to salt-tolerance of P. cornutum were identified； however， the expression of regulatory genes responding to salt stress in P. cornutum have not been reported. In order to further explore the molecular mechanism concerning salt tolerance of P. cornutum， in this study， the expression pattern of protein kinase related genes in P. cornutum under salt treatment was analyzed， based on the transcriptomic data of root and shoot tissues of P. cornutum exposed to 50 mmol·L^-1 NaCl for 6 and 24 h. The results showed that there were significant changes in the expression of a large number of protein kinase related genes in P. cornutum under 50 mmol·L^-1 NaCl. Among them， the expression of numerous genes encoding leucine-rich repeat receptor protein kinases （LRR-RLKs） were significantly up-regulated in both the root and shoot of P. cornutum after treatment with 50 mmol·L^-1 NaCl for both 6 and 24 h； many genes related to mitogen-activated protein kinase cascade pathways （MAPK/MAPKK/MAPKKK） were significantly up-regulated under short-term salt stress （50 mmol·L^-1 NaCl for 6 h）. Some genes encoding CTR1， an important negative regulator of the ethylene signal transduction pathway， were expressed in roots of P. cornutum under control condition but not expressed in roots under 50 mmol·L^-1 NaCl treatment for 6 h. These results indicate that LRR-RLK proteins may play an important regulatory role in the adaptation of P. cornutum to salt stress， MAPK/MAPKK/MAPKKK may be involved in regulating the response of P. cornutum to short-term salt stress， and CTR1 might function as a negative regulator in the response of P. cornutum to salt stress.

Key words: Pugionium cornutum, salt stress, protein kinase, differentially expressed genes （DEGs）

Fang-zhen WANG, Cheng-hang YANG, Zi-hua HE, Zi-ru LIN, Hao-yuan ZENG, Qing MA. Analysis of differentially expressed protein kinase related genes in the xerophyte Pugionium cornutum under salt treatment[J]. Acta Prataculturae Sinica, 2021, 30(10): 116-124.

Figures/Tables 9

References 31

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基因Gene	基因编号Gene ID	同源基因Homologous gene	同源物种Homologous species
LRR-RLKs	CL2242.Contig2_All	LRR-RLK （AT5G10020）	拟南芥Arabidopsis thaliana
	CL797.Contig29_All	LRR-RLK （AT3G49670）	亚麻荠Camelina sativa
	CL6717.Contig2_All	LRR-RLK （AT1G29720）	拟南芥A. thaliana
	CL7990.Contig5_All	LRR-RLK （AT3G14350 ）	拟南芥A. thaliana
	Unigene29560_All	LRR-RLK （AT5G07150）	拟南芥A. thaliana
	CL1136.Contig10_All	LRR-RLK （AT1G07560）	拟南芥A. thaliana
WAK	CL807.Contig2_All	WAK 4	拟南芥A. thaliana
EDR1	CL4341.Contig2_All	EDR1	拟南芥A. thaliana
CK	CL1663.Contig1_All	CK II	拟南芥A. thaliana
MAPK/MAPKK/MAPKKK	CL2088.Contig2_All	MAPK 5	拟南芥A. thaliana
MAPK/MAPKK/MAPKKK	CL1291.Contig1_All	MAPK18	拟南芥A. thaliana

基因Gene	基因编号Gene ID	同源基因Homologous gene	同源物种Homologous species
LRR-RLKs	CL2242.Contig2_All	LRR-RLK （AT5G10020）	拟南芥Arabidopsis thaliana
	CL797.Contig29_All	LRR-RLK （AT3G49670）	亚麻荠Camelina sativa
	CL6717.Contig2_All	LRR-RLK （AT1G29720）	拟南芥A. thaliana
	CL7990.Contig5_All	LRR-RLK （AT3G14350 ）	拟南芥A. thaliana
	Unigene29560_All	LRR-RLK （AT5G07150）	拟南芥A. thaliana
	CL1136.Contig10_All	LRR-RLK （AT1G07560）	拟南芥A. thaliana
WAK	CL807.Contig2_All	WAK 4	拟南芥A. thaliana
EDR1	CL4341.Contig2_All	EDR1	拟南芥A. thaliana
CK	CL1663.Contig1_All	CK II	拟南芥A. thaliana
MAPK/MAPKK/MAPKKK	CL2088.Contig2_All	MAPK 5	拟南芥A. thaliana
MAPK/MAPKK/MAPKKK	CL1291.Contig1_All	MAPK18	拟南芥A. thaliana

处理时间Treatments time （h）	基因编号Gene ID	同源基因Homologous gene	同源物种Homologous species
6	CL3516.Contig2_All	CTR1	拟南芥A. thaliana
6	CL2980.Contig2_All	CTR1	琴叶拟南芥Arabidopsis lyrata
24	CL2548.Contig11_All	CTR1	拟南芥A. thaliana
	CL2980.Contig4_All	CTR1	琴叶拟南芥A. lyrata
	CL3385.Contig7_All	CTR1	琴叶拟南芥A. lyrata

处理时间Treatments time （h）	基因编号Gene ID	同源基因Homologous gene	同源物种Homologous species
6	CL3516.Contig2_All	CTR1	拟南芥A. thaliana
6	CL2980.Contig2_All	CTR1	琴叶拟南芥Arabidopsis lyrata
24	CL2548.Contig11_All	CTR1	拟南芥A. thaliana
	CL2980.Contig4_All	CTR1	琴叶拟南芥A. lyrata
	CL3385.Contig7_All	CTR1	琴叶拟南芥A. lyrata

基因Gene	基因编号Gene ID	同源基因Homologous gene	同源物种Homologous species
LRR-RLKs	CL797.Contig15_All	LRR-RLK （AT3G49670）	拟南芥A. thaliana
	CL2242.Contig1_All	LRR-RLK （AT5G10020）	拟南芥A. thaliana
	CL217.Contig3_All	LRR-RLK （AT1G07650）	拟南芥A. thaliana
CDPK	CL5176.Contig7_All	CDPK5	拟南芥A. thaliana
CDPK	Unigene12783_All	CDPK6	拟南芥A. thaliana
CK	Unigene2819_All	CKI	拟南芥A. thaliana