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Acta Prataculturae Sinica ›› 2021, Vol. 30 ›› Issue (10): 105-115.DOI: 10.11686/cyxb2020375

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Expression characteristics and functional analysis of the LcCBF6 gene from Leymus chinensis

Qian LI1,2(), Xiao-xia LI1(), Li-qin CHENG1, Shuang-yan CHEN1, Dong-mei QI1, Wei-guang YANG3, Li-jun GAO1, Ba-yin XIN1, Gong-she LIU1()   

  1. 1.Key Laboratory of Plant Resources,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China
    2.Shandong Zibo No. 7 Middle School,Zibo 255000,China
    3.Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences,Qiqihar 161005,China
  • Received:2020-08-04 Revised:2020-09-21 Online:2021-09-16 Published:2021-09-16
  • Contact: Xiao-xia LI,Gong-she LIU


Chinese wild ryeLeymus chinensis) is an important forage grass in China, and is also ecologically important because it has high tolerance to saline-alkali soil, drought, and low temperature stresses. Chinese wild rye can therefore be an excellent gene pool for molecular breeding to enhance stress tolerance. CBF/DREBs belong to the AP2 transcription factor family and play an important role in plant stress resistance. In this study, the LcCBF6(L. chinensis C-repeat binding factor 6) gene was cloned. It encoded 245 amino acids and contained the AP2 domain. Amino acid sequence alignment showed that the LcCBF6 gene had high sequence conformity to its homologous proteins from Agropyron mongolicum (92%) and Secale cereale (91%). Expression analysis confirmed that LcCBF6 gene was expressed in roots, leaves and seeds, and was significantly induced by salt stress. Overexpression of LcCBF6 significantly enhanced the salt resistance of transgenic Arabidopsis thaliana. Under the salt stress, the number of green cotyledons, the root length, plant biomass and survival rate of transgenic lines were all significantly higher than in the wild type. The results suggest that the LcCBF6 gene might play an important positive role in salt stress tolerance, and be an excellent gene to utilize for enhancing stress tolerance in forages and other important crops.

Key words: Leymus chinensis, CBF gene family, LcCBF6, transgene, salt tolerance