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Acta Prataculturae Sinica ›› 2023, Vol. 32 ›› Issue (2): 119-130.DOI: 10.11686/cyxb2022044

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Effects of exogenous hydrogen sulfide on amino acid metabolism in naked oat leaves under saline-alkali stress

Jian-xin LIU(), Rui-rui LIU, Xiu-li LIU, Xiao-bin OU, Hai-yan JIA, Ting BU, Na LI   

  1. Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration,College of Life Sciences and Technology,Longdong University,Qingyang 745000,China
  • Received:2022-01-22 Revised:2022-03-09 Online:2023-02-20 Published:2022-12-01
  • Contact: Jian-xin LIU

Abstract:

The mechanism where by hydrogen sulfide alleviates saline-alkali stress in plants has attracted much attention. This experiment investigated the mechanisms underlying exogenous hydrogen sulfide effects on the amino acid metabolism of plants under saline-alkali stress in potted plants of the naked oat variety ‘Dingyou 9’ grown in soil. There were four treatments: a 2×2 factorial combination of no saline-alkali or 3.00 g·kg-1 saline-alkali (molar ratio NaCl∶Na2SO4∶Na2CO3∶NaHCO3=12∶8∶1∶9) added to the soil in the pot, and the leaves were sprayed with distilled water or 50 μmol·L-1 sodium hydrosulfide (a hydrogen sulfide donor, NaHS) solution at the heading stage. Effects of exogenous hydrogen sulfide on the total amino acid and malondialdehyde contents in leaves and on the grain yield of naked oats under the four treatments were studied. Liquid chromatography and mass spectrometry were used for chemical analysis, and data were analyzed by principal component analysis of levels of the 22 amino acids that make up the protein, in order to determine the regulatory effect of exogenous hydrogen sulfide on amino acid metabolic pathways. It was found that: homocysteine was not detected in the leaves of naked oat. There was no significant decrease in total amino acid content in naked oat leaves under saline-alkali stress as a result of spraying NaHS, but NaHS significantly alleviated the increase in malondialdehyde content in leaves and the decrease in grain yield induced by saline-alkali stress. The results of principal component analysis showed that under saline-alkali stress, spraying NaHS solution significantly down-regulated the contents of ornithine (α-ketoglutarate family) and asparagine (oxaloacetate family), and significantly up-regulated the contents of glutamine, proline, arginine (α-ketoglutarate family), leucine (pyruvate family), and tyrosine (aromatic family) in leaves, but had no significant effect on the contents of glycine, serine (3-phosphoglycerate family), tryptophan, phenylalanine acid (aromatic family), alanine, valine (pyruvate family), 4-aminobutyric acid, histidine, glutamic acid (α-ketoglutarate family), isoleucine, threonine, methionine, lysine and aspartic acid (oxaloacetic acid family). The above results indicate that exogenous hydrogen sulfide is involved in the regulation of amino acid metabolic pathways in naked oat under saline-alkali stress, and that NaHS can alleviate the oxidative damage and inhibition of assimilate accumulation caused by saline-alkali stress.

Key words: saline-alkali stress, hydrogen sulfide, naked oats, amino acid metabolism