Effect of grazing exclusion on soil organic carbon and stoichiometry characteristics of soil microbial biomass in sagebrush desert

doi:10.11686/cyxb2022267

Abstract

Abstract:

This research aimed to clarify soil microbial biomass eco-stoichiometry characteristics in desert montane grassland in the years following grazing exclusion. Soil organic carbon accumulation and its relationship in degraded desert grassland to soil organic carbon （SOC）， soil microbial biomass carbon （MBC）， nitrogen （MBN）， phosphorus （MBP）and their stoichiometric ratios were analyzed in the 0-50 cm soil layer of sagebrush desert grasslands from Xinyuan， Bole， Manasi， Hutubi and Qitai counties on the northern slopes of the Tianshan Mountains for 4-7 years following grazing exclusion. The relationships between soil organic carbon （SOC）， soil MBC， MBN， MBP and their stoichiometric ratios were analyzed by structural equation modeling （SEM）. It was found that the SOC content of sagebrush desert grassland in the 0-50 cm soil horizon after grazing exclusion was significantly reduced by 15.52% compared with the control （P<0.05）， while the overall changes of soil MBC， MBN， MBP， MBC∶MBN， MBC∶MBP and MBN∶MBP were not significant. SOC was significantly positively correlated with MBC， MBN， and MBP （P<0.01）， and negatively correlated with MBC∶MBN and MBC∶MBP， and the relationship between SOC and MBN∶MBP changed from significantly positive to negative after grazing exclusion （P<0.01）. SEM showed that soil MBC， MBN， MBP and their stoichiometric ratios data directly explained 46% of data variation for SOC accumulation， and MBN had the strongest effect on soil organic carbon （P<0.001）， while grazing exclusion reduced the effect of soil MBC and MBN on soil organic carbon accumulation and increased the direct effect of soil MBP. In conclusion， the effects of 4-7 years of grazing exclusion on soil microbial biomass eco-stoichiometry characteristics of sagebrush desert grassland were not significant， and MBN was the most influential determining factor for soil organic carbon accumulation.

Key words: sagebrush desert, soil organic carbon, soil microbial biomass, stoichiometric characteristics, grazing exclusion

Si-yuan LI, Yu-xuan CUI, Zong-jiu SUN, Hui-xia LIU, Hua-wei YE. Effect of grazing exclusion on soil organic carbon and stoichiometry characteristics of soil microbial biomass in sagebrush desert[J]. Acta Prataculturae Sinica, 2023, 32(6): 58-70.

Figures/Tables 10

References 48

1	Millard P， Singh B K. Does grassland vegetation drive soil microbial diversity？ Nutrient Cycling in Agroecosystems， 2010， 88（2）： 147-158.
2	Yang Y， Li H， Zhang L， et al. Characteristics of soil water percolation and dissolved organic carbon leaching and their response to long-term fencing in an alpine meadow on the Tibetan Plateau. Environmental Earth Sciences， 2016， 75（23）： 1-10.
3	Martensson L M， Olsson P A. Reductions in microbial biomass along disturbance gradients in a semi-natural grassland. Applied Soil Ecology， 2012， 62： 8-13.
4	Chu H Y. Microbial communities in high latitudes and high altitudes ecosystems. Microbiology China， 2013， 40（1）： 123-136.
	褚海燕. 高寒生态系统微生物群落研究进展. 微生物学通报， 2013， 40（1）： 123-136.
5	Peng X Q， Wang W. Spatial pattern of soil microbial biomass carbon and its driver in temperate grasslands of Inner Mongolia. Microbiology China， 2016， 43（9）： 1918-1930.
	彭晓茜，王娓. 内蒙古温带草原土壤微生物生物量碳的空间分布及驱动因素. 微生物学通报， 2016， 43（9）： 1918-1930.
6	Liu S L， Su Y R， Huang D Y， et al. Response of C_mic-to-C_org to land use and fertilization in subtropical region of China. Scientia Agricultura Sinica， 2006， 39（7）： 1411-1418.
	刘守龙，苏以荣，黄道友，等. 微生物商对亚热带地区土地利用及施肥制度的响应. 中国农业科学， 2006， 39（7）： 1411-1418.
7	Manzoni S， Trofymow J A， Jackson R B， et al. Stoichiometric controls on carbon， nitrogen， and phosphorus dynamics in decomposing litter. Ecological Monographs， 2010， 80（1）： 89-106.
8	Heuch C， Weig A， Spohn M. Soil microbial biomass C∶N∶P stoichiometry and microbial use of organic phosphorus. Soil Biology & Biochemistry， 2015， 85： 119-129.
9	Oduor C O， Karanja N K， Onwonga R N， et al. Enhancing soil organic carbon， particulate organic carbon and microbial biomass in semi-arid rangeland using pasture enclosures. BMC Ecology， 2018， 18（1）： 1-9.
10	Zhang W L， Kolbe H， Zhang R L. Research progress of SOC functions and transformation mechanisms. Scientia Agricultura Sinica，2020， 53（2）： 317-331.
	张维理， Kolbe H，张认连. 土壤有机碳作用及转化机制研究进展. 中国农业科学， 2020， 53（2）： 317-331.
11	Xu P. Grassland resources and their utilization in Xinjiang. Urumqi： Xinjiang Science and Technology Health Press， 1993.
	许鹏. 新疆草地资源及其利用. 乌鲁木齐：新疆科技卫生出版社， 1993.
12	Cui Y X， Sun Z J， Liu H X， et al. Effects of short-term grazing exclusion on standing biomass and plant community diversity in sagebrush desert. Acta Prataculturae Sinica， 2020， 29（12）： 17-26.
	崔雨萱，孙宗玖，刘慧霞，等. 短期封育对蒿类荒漠草地现存生物量及植物群落多样性的影响. 草业学报， 2020， 29（12）： 17-26.
13	Zhu H， Ke M， Li X S， et al. Effect of grazing on plant communities and soil physical and chemical properties of Artemisia desert grassland. Grassland and Turf， 2017， 37（4）： 68-73.
	朱昊，柯梅，李学森，等. 放牧对蒿类荒漠草地植物群落和土壤理化性质的影响. 草原与草坪， 2017， 37（4）： 68-73.
14	Dong Y Q， An S Z， Sun Z J， et al. Effects of grazing exclusion times on soil organic carbon storage and microbial biomass carbon and nitrogen in degraded Seriphidium transiliense desert. Xinjiang Agricultural Sciences， 2017， 54（5）： 961-968.
	董乙强，安沙舟，孙宗玖，等. 禁牧年限对退化伊犁绢蒿荒漠土壤有机碳库和微生物碳，氮的影响. 新疆农业科学， 2017， 54（5）： 961-968.
15	Li J H， Li Z Q， Ren J Z. The effects of grazing on grassland plants. Acta Prataculturae Sinica， 2002， 11（1）： 4-11.
	李金花，李镇清，任继周. 放牧对草原植物的影响. 草业学报， 2002， 11（1）： 4-11.
16	Liu F C， Li H L， Dong Z， et al. Advances in research on enclosure effects on vegetation restoration and soil physicochemical property of degraded grassland. Science of Soil and Water Conservation， 2012， 10（5）： 116-122.
	刘凤婵，李红丽，董智，等. 封育对退化草原植被恢复及土壤理化性质影响的研究进展. 中国水土保持科学， 2012， 10（5）： 116-122.
17	Qin L P， Bai W L， Zheng T J. Research progress on the effect of fencing on grassland soil nutrient improvement. China Cattle Science， 2020， 46（6）： 20-23.
	秦丽萍，白文丽，郑廷杰. 围栏封育对草地土壤养分改良效果的研究进展. 中国牛业科学， 2020， 46（6）： 20-23.
18	Cheng Y T. Research on grassland vegetation and soil recover after fencing in China： A meta-analvsis. Shanghai： East China Normal University， 2020.
	程雨婷. 围栏封育后我国草地植被与土壤恢复的Meta分析研究. 上海：华东师范大学， 2020.
19	Li X L， Zhang D， Yin H L， et al. Effects of short-term enclosure on soil carbon， nitrogen and phosphorus contents in sandy elm sparse forest steppe. Journal of Chifeng University （Natural Science）， 2021， 37（7）： 40-45.
	李晓兰，张丹，尹慧来，等. 短期围封对沙地榆树疏林草原土壤碳氮磷含量的影响. 赤峰学院学报（自然科学版）， 2021， 37（7）： 40-45.
20	Asitaiken J L H T， Dong Y Q， Li J， et al. Effects of grazing exclusion on nutrition and stoichiometry characteristics of Artemisia desert vegetation and soil. Journal of Arid Land Resources and Environment， 2021， 35（11）： 157-164.
	阿斯太肯·居力海提，董乙强，李靖，等. 禁牧对不同气候区蒿类荒漠植被和土壤养分及化学计量特征的影响. 干旱区资源与环境， 2021， 35（11）： 157-164.
21	Reeder J， Schuman G E. Influence of livestock grazing on C sequestration in semi-arid mixed-grass and short-grass rangelands. Environmental Pollution， 2002， 116（3）： 457-463.
22	Fan Y M， Wu H Q， Sun Z J， et al. Effects of fencing on the soil organic carbon of desert grassland in the northern slope of Tianshan. Acta Agrestia Sinica， 2014， 22（1）： 65-69.
	范燕敏，武红旗，孙宗玖，等. 围封对天山北坡荒漠草地土壤有机碳的影响. 草地学报， 2014， 22（1）： 65-69.
23	Jing J， Zhang M Y， Gao Y H. Effects of enclosure on soil microbial carbon utilization in an alpine steppe. Ecological Science， 2021， 40（3）： 25-32.
	敬洁，张梦瑶，高永恒. 围栏禁牧对高寒草原土壤微生物碳源利用的影响. 生态科学， 2021， 40（3）： 25-32.
24	Dong Y Q， Sun Z J， An S Z， et al. Effects of grazing exclusion on soil nutrition in moderate degraded desert grassland of Seriphidium transiliense. Pratacultural Science， 2016， 33（8）： 1460-1468.
	董乙强，孙宗玖，安沙舟，等. 禁牧对中度退化伊犁绢蒿荒漠草地土壤养分的影响. 草业科学， 2016， 33（8）： 1460-1468.
25	He Y T， Dong Y S， Qi Y C， et al. Advances in researches on soil microbial biomass of grassland ecosystems and its influencing factors. Progress in Geography， 2010， 29（11）： 1350-1359.
	何亚婷，董云社，齐玉春，等. 草地生态系统土壤微生物量及其影响因子研究进展. 地理科学进展， 2010， 29（11）： 1350-1359.
26	Bao S D. Soil and agricultural chemistry analysis （Third Edition）. Beijing： China Agriculture Press， 2000.
	鲍士旦. 土壤农化分析（第三版）. 北京：中国农业出版社， 2000.
27	Brookes P C， Powlson D S， Jenkinson D S. Measurement of microbial biomass phosphorus in soil. Soil Biology and Biochemistry， 1982， 14（4）： 319-329.
28	Shi F， Li Y E， Gao Q Z， et al. Effects of managements on soil organic carbon of grassland in China. Pratacultural Science， 2009， 26（3）： 9-15.
	石锋，李玉娥，高清竹，等. 管理措施对我国草地土壤有机碳的影响. 草业科学， 2009， 26（3）： 9-15.
29	Xue K， Zhang B， Zhou S T， et al. Soil microbial communities in alpine grasslands on the Tibetan Plateau and their influencing factors. Chinese Science Bulletin， 2019， 64（27）： 2915-2927.
	薛凯，张彪，周姝彤，等. 青藏高原高寒草地土壤微生物群落及影响因子. 科学通报， 2019， 64（27）： 2915-2927.
30	Hu Y L， Wu X F. Discussion on soil microbial biomass as a bio-indicator of soil quality for latosol earth. Journal of Central South Forestry University， 2002， 22（3）： 51-53.
	胡曰利，吴晓芙. 土壤微生物生物量作为土壤质量生物指标的研究. 中南林学院学报， 2002， 22（3）： 51-53.
31	Liu Y F， Tian Y W. Ecological stoichiometry characteristics of soil and microbial biomass C， N and P in vineyards with different planting years. Journal of Henan Agricultural Sciences， 2021， 50（8）： 57-65.
	刘谊锋，田耀武. 不同种植年限葡萄园土壤及微生物量碳，氮，磷生态化学计量特征. 河南农业科学， 2021， 50（8）： 57-65.
32	Cao C Y， Shao J F， Jiang D M， et al. Effects of fence enclosure on soil nutrients and biological activities in highly degraded grasslands. Journal of Northeastern University （Natural Science）， 2011， 32（3）： 427-430.
	曹成有，邵建飞，蒋德明，等. 围栏封育对重度退化草地土壤养分和生物活性的影响. 东北大学学报（自然科学版）， 2011， 32（3）： 427-430.
33	Yin Y L， Wang Y Q， Li S X， et al. Effects of enclosing on soil microbial community diversity and soil stoichiometric characteristics in a degraded alpine meadow. Chinese Journal of Applied Ecology， 2019， 30（1）： 127-136.
	尹亚丽，王玉琴，李世雄，等. 围封对退化高寒草甸土壤微生物群落多样性及土壤化学计量特征的影响. 应用生态学报， 2019， 30（1）： 127-136.
34	Wang C Y， Zhang J J， Lv Y L， et al. Effects of long-term grazing exclusion on soil organic carbon fractions in the grasslands of Inner Mongolia. Acta Prataculturae Sinica， 2014， 23（5）： 31-39.
	王春燕，张晋京，吕瑜良，等. 长期封育对内蒙古羊草草地土壤有机碳组分的影响. 草业学报， 2014， 23（5）： 31-39.
35	Zhu X Y， Li Z H， Zhao X R， et al. Differences of soil microbial biomass C， N and P contents in typical grasslands of Inner Mongolia under different grazing intensities. Journal of China Agricultural University， 2020， 25（9）： 121-130.
	朱晓亚，李子豪，赵小蓉，等. 连续4年不同放牧强度内蒙古典型草原土壤微生物量碳，氮，磷含量差异. 中国农业大学学报， 2020， 25（9）： 121-130.
36	Chen H. Impact of water status on soil microbial biomass and community structure in typical grasslands of Inner Mongolia. Beijing： China Agricultural University， 2018.
	陈昊. 水分状况对内蒙古典型草原土壤微生物量及群落结构的影响. 北京：中国农业大学， 2018.
37	Liu N， Zhang Y， Chang S， et al. Impact of grazing on soil carbon and microbial biomass in typical steppe and desert steppe of Inner Mongolia. PLoS One， 2012， 7（5）： e36434.
38	Cleveland C C， Liptzin D. C∶N∶P stoichiometry in soil： Is there a “Redfield ratio” for the microbial biomass？ Biogeochemistry， 2007， 85（3）： 235-252.
39	Liu M Q， Hu F， He Y Q， et al. Seasonal dynamics of soil microbial biomass and its significance to indicate soil quality under different vegetations restored on degraded red soils. Acta Pedologica Sinica， 2003， 40（6）： 937-944.
	刘满强，胡锋，何园球，等. 退化红壤不同植被恢复下土壤微生物量季节动态及其指示意义. 土壤学报， 2003， 40（6）： 937-944.
40	Xu X， Thornton P E， Post W M. A global analysis of soil microbial biomass carbon， nitrogen and phosphorus in terrestrial ecosystems. Global Ecology and Biogeography， 2013， 22（6）： 737-749.
41	Zheng H， Xue J B， Gui J H， et al. Short-term effects of grazing intensity on soil stoichiometric characteristics of typical grass-land in the agro-pastoral ecotone of Northern China. Chinese Journal of Applied Ecology， 2021， 32（7）： 2433-2439.
	郑慧，薛江博，桂建华，等. 放牧强度对华北农牧交错带典型草地土壤化学计量特征的短期影响. 应用生态学报， 2021， 32（7）： 2433-2439.
42	Wang C J， Wang Q Q， Xu H， et al. Carbon， nitrogen， and phosphorus stoichiometry characteristics of bulk soil， organic matter， and soil microbial biomass under long-term fertilization in cropland. Acta Ecologica Sinica， 2018， 38（11）： 3848-3858.
	王传杰，王齐齐，徐虎，等. 长期施肥下农田土壤-有机质-微生物的碳氮磷化学计量学特征. 生态学报， 2018， 38（11）： 3848-3858.
43	Pan Y， Fang F， Tang H. Patterns and internal stability of carbon， nitrogen， and phosphorus in soils and soil microbial biomass in terrestrial ecosystems in China： A data synthesis. Forests， 2021， 12（11）： 1544.
44	Chen Y L， Chen L Y， Peng Y F， et al. Linking microbial C∶N∶P stoichiometry to microbial community and abiotic factors along a 3500‐km grassland transect on the Tibetan Plateau. Global Ecology and Biogeography， 2016， 25（12）： 1416-1427.
45	Zhou C N， Ma H P. Distribution of labile organic carbon in soil as affected by vegetation typical of Sygera mountains， Tibet， China. Acta Pedologica Sinica， 2013， 50（6）： 1246-1251.
	周晨霓，马和平. 西藏色季拉山典型植被类型土壤活性有机碳分布特征. 土壤学报， 2013， 50（6）： 1246-1251.
46	Fierer N， Schimel J P， Holden P A. Variations in microbial community composition through two soil depth profiles. Soil Biology and Biochemistry， 2003， 35（1）： 167-176.
47	Zhou Z H， Wang C K. Soil-microbe-mineralization carbon and nitrogen stoichiometry under different land-uses in the Maoershan region. Acta Ecologica Sinica， 2017， 37（7）： 2428-2436.
	周正虎，王传宽. 帽儿山地区不同土地利用方式下土壤-微生物-矿化碳氮化学计量特征. 生态学报， 2017， 37（7）： 2428-2436.
48	Wang Y， Sun J， Ye C C， et al. Climatic factors drive the aboveground ecosystem functions of alpine grassland via soil microbial biomass nitrogen on the Qingzang Plateau. Chinese Journal of Plant Ecology， 2021， 45（5）： 434-443.
	王毅，孙建，叶冲冲，等. 气候因子通过土壤微生物生物量氮促进青藏高原高寒草地地上生态系统功能. 植物生态学报， 2021， 45（5）： 434-443.

样地 Site	新源县 Xinyuan （XY）	博乐市 Bole （BL）	玛纳斯县 Manasi （MNS）	呼图壁县 Hutubi （HTB）	奇台县 Qitai （QT）
地理位置 Geographical position	43°22′ N，82°36′ E	44°50′ N，81°39′ E	44°01′ N，86°09′ E	43°58′ N，86°32′ E	43°47′ N，89°25′ E
年均降水量 Mean annual precipitation （mm）	204	200	172	224	160
年均气温 Mean annual temperature （℃）	7.13	6.10	8.18	6.79	7.17
海拔 Altitude （m）	887	1110	1033	978	1227
封育开始时间Start time of grazing exclusion （Year/month）	2012/6	2014/7	2015/7	2015/7	2012/6
土壤基质 Soil texture	土质 Soil	砾石质 Gravelly	土质 Soil	土质 Soil	土质 Soil
优势种 Dominant species	伊犁绢蒿S. transiliense	博洛塔绢蒿，木地肤S. borotalalense，K. prostrata	伊犁绢蒿，叉毛蓬S. transiliense，P. sibirica	伊犁绢蒿，叉毛蓬S. transiliense，P. sibirica	伊犁绢蒿，木地肤，短柱苔草S. transiliense，K. prostrata，C. turkestanica

样地 Site	新源县 Xinyuan （XY）	博乐市 Bole （BL）	玛纳斯县 Manasi （MNS）	呼图壁县 Hutubi （HTB）	奇台县 Qitai （QT）
地理位置 Geographical position	43°22′ N，82°36′ E	44°50′ N，81°39′ E	44°01′ N，86°09′ E	43°58′ N，86°32′ E	43°47′ N，89°25′ E
年均降水量 Mean annual precipitation （mm）	204	200	172	224	160
年均气温 Mean annual temperature （℃）	7.13	6.10	8.18	6.79	7.17
海拔 Altitude （m）	887	1110	1033	978	1227
封育开始时间Start time of grazing exclusion （Year/month）	2012/6	2014/7	2015/7	2015/7	2012/6
土壤基质 Soil texture	土质 Soil	砾石质 Gravelly	土质 Soil	土质 Soil	土质 Soil
优势种 Dominant species	伊犁绢蒿S. transiliense	博洛塔绢蒿，木地肤S. borotalalense，K. prostrata	伊犁绢蒿，叉毛蓬S. transiliense，P. sibirica	伊犁绢蒿，叉毛蓬S. transiliense，P. sibirica	伊犁绢蒿，木地肤，短柱苔草S. transiliense，K. prostrata，C. turkestanica

封育 Grazing exclusion	对照Control
封育 Grazing exclusion	SOC	MBC	MBN	MBP	MBC/MBN	MBC/MBP	MBN/MBP
SOC	1.000	0.628**	0.745**	0.647**	-0.182*	-0.135	0.206**
MBC	0.428**	1.000	0.838**	0.746**	-0.217**	-0.068	0.187*
MBN	0.572**	0.798**	1.000	0.871**	-0.392**	-0.182*	0.283**
MBP	0.553**	0.730**	0.847**	1.000	-0.319**	-0.305**	0.017
MBC/MBN	-0.274**	-0.259**	-0.408**	-0.348**	1.000	0.279**	-0.449**
MBC/MBP	-0.245**	-0.181*	-0.237**	-0.291**	0.793**	1.000	0.442**
MBN/MBP	-0.102	-0.021	0.067	-0.151	-0.073	0.415**	1.000

封育 Grazing exclusion	对照Control
封育 Grazing exclusion	SOC	MBC	MBN	MBP	MBC/MBN	MBC/MBP	MBN/MBP
SOC	1.000	0.628**	0.745**	0.647**	-0.182*	-0.135	0.206**
MBC	0.428**	1.000	0.838**	0.746**	-0.217**	-0.068	0.187*
MBN	0.572**	0.798**	1.000	0.871**	-0.392**	-0.182*	0.283**
MBP	0.553**	0.730**	0.847**	1.000	-0.319**	-0.305**	0.017
MBC/MBN	-0.274**	-0.259**	-0.408**	-0.348**	1.000	0.279**	-0.449**
MBC/MBP	-0.245**	-0.181*	-0.237**	-0.291**	0.793**	1.000	0.442**
MBN/MBP	-0.102	-0.021	0.067	-0.151	-0.073	0.415**	1.000

[1]	Xin GUO, Huan LUO, Xue-mei XU, Ai-xia MA, Zhen-yan SHANG, Tian-hu HAN, De-cao NIU, Hai-yan WEN, Xu-dong LI. Effects of litter decomposition with different qualities on soil organic carbon content and its stability in grassland on the Loess Plateau [J]. Acta Prataculturae Sinica, 2023, 32(5): 83-93.
[2]	Yang LI, Yi WANG, Guo-dong HAN, Jian SUN, Ya-feng WANG. Soil microbial biomass carbon and nitrogen levels and their controlling factors in alpine grassland， Qinghai-Tibet Plateau [J]. Acta Prataculturae Sinica, 2022, 31(6): 50-60.
[3]	Xiao-yu HAN, Ning GUO, Dong-dong LI, Ming-yang XIE, Feng JIAO. Effects of nitrogen addition on soil carbon and nitrogen and biomass change in different grassland types in Inner Mongolia [J]. Acta Prataculturae Sinica, 2022, 31(1): 13-25.
[4]	Xing WANG, Shuang YU, Dong-mei XU, Ke-chen SONG. Effects of different restorative measures on soil carbon and nitrogen and their component fractions in a degraded desert steppe [J]. Acta Prataculturae Sinica, 2022, 31(1): 26-35.
[5]	Ji-xiong GU, Tian-dou GUO, Hong-mei WANG, Xue-ying LI, Dan-ni LIANG, Qing-lian YANG, Jin-yue GAO. Responses of soil microbes across an anthropogenic transition from desert steppe grassland to shrubland in eastern Ningxia [J]. Acta Prataculturae Sinica, 2021, 30(4): 46-57.
[6]	Shuai-nan LIU, Guang LI, Jiang-qi WU, Wei-wei MA, Chuan-jie YANG, Shi-kang ZHANG, Yao YAO, Yan-hua LU, Xing-xing WEI, Juan ZHANG. Characteristics of soil nutrients under different land types in the loess hill region based on ecological chemometrics [J]. Acta Prataculturae Sinica, 2021, 30(3): 200-207.
[7]	Hui-xia LIU, Yi-qiang DONG, Yu-xuan CUI, Xing-hong LIU, Pan-xing HE, Qiang SUN, Zong-jiu SUN. Environmental factors influencing soil organic carbon and its characteristics in desert grassland in Altay， Xinjiang [J]. Acta Prataculturae Sinica, 2021, 30(10): 41-52.
[8]	Shi-jing ZHOU, Jia-ning LUO, Zhong-miao LIU, Chao DONG, Yan QIN, Shu-juan WU, Hong-jun GAN, Fei XIE, Guang-hui ZHUANG, Bing-zhe FU, De-cao NIU. The effects of Vicia sativa planting density on soil microbial nutrient metabolism [J]. Acta Prataculturae Sinica, 2021, 30(10): 63-72.
[9]	Bo JI, Jian-long HE, Xu-dong WU, Zhan-jun WANG, Ying-zhong XIE, Qi JIANG. Characteristics of soil organic carbon and active organic carbon in typical natural grassland in Ningxia [J]. Acta Prataculturae Sinica, 2021, 30(1): 24-35.
[10]	BAO Gen-sheng, SONG Mei-ling, WANG Yu-qin, YIN Ya-li, WANG Hong-sheng. Effects of grazing exclosure and herbicide on soil physical-chemical properties and microbial biomass of Stellera chamaejasme patches in degraded grassland [J]. Acta Prataculturae Sinica, 2020, 29(9): 63-72.
[11]	Yu-xuan CUI, Zong-jiu SUN, Hui-xia LIU, Yi-qiang DONG. Effects of short-term grazing exclusion on standing biomass and plant community diversity in sagebrush desert [J]. Acta Prataculturae Sinica, 2020, 29(12): 17-26.
[12]	WANG Xiao-jiao, QI Peng, CAI Li-qun, CHEN Xiao-long, XIE Jun-hong, GAN Hui-jiong, ZHANG Ren-zhi. Effects of alternative fertilization practices on components of the soil organic carbon pool and yield stability in rain-fed maize production on the Loess Plateau [J]. Acta Prataculturae Sinica, 2020, 29(10): 58-69.
[13]	WANG Li-na, LUO Jiu-fu, YANG Mei-xiang, ZHANG Li, LIU Xue-min, DENG Dong-zhou, ZHOU Jin-xing. Effects of nitrogen addition on the soil microbial biomass C and microbial biomass N in degraded alpine grassland in Zoige County [J]. Acta Prataculturae Sinica, 2019, 28(7): 38-48.
[14]	YU Shuang, XU Dong-mei, XU Ai-yun, LIU Jin-long, TAO Li-bo. Effects of different restoration measures on the soil organic carbon and nitrogen reserves in a desert steppe grassland ecosystem in Ningxia [J]. Acta Prataculturae Sinica, 2019, 28(3): 12-19.
[15]	ZHANG Miao-miao, CHEN Wei, LIN Li, ZHANG De-gang, WU Yu-xin, XIAO Hai-long. A study of soil nutrient characteristics and soil soluble organic carbon levels in different types of alpine grassland in Qinghai Province [J]. Acta Prataculturae Sinica, 2019, 28(3): 20-28.