Identification and expression pattern of the WRKY transcription factor family in Medicago sativa

doi:10.11686/cyxb2023214

Abstract

Abstract:

WRKY transcription factors are one of the largest gene families in plants and play important roles in plant growth and development as well as in abiotic stress processes. Currently， members of the WRKY gene family have been extensively studied and analyzed in a variety of plant species. However， the alfalfa （Medicago sativa） WRKY transcription factors have not been identified and analyzed at the genome-wide level. In this study， we systematically identified alfalfa WRKY gene family members and analyzed their expression patterns under different stress treatments. We showed that a total of 198 WRKY transcription factors were unevenly distributed across 32 chromosomes in alfalfa， with chromosome 7 having the largest number. The phylogenetic tree results show that the WRKY gene family members can be divided into three groups. Among these WRKY transcription factors， 42 responded to salt stress， 7 to alkali stress， 19 to cold stress and 37 to drought stress， and MsWRKY154 and MsWRKY166 were found to respond to both salt， drought， and cold as well as alkali stress. The results of this study provide a foundation for the intensive investigation of the biological function of WRKY transcription factors in alfalfa， and can also provide valuable information for molecular breeding in alfalfa.

Key words: WRKY transcription factor, alfalfa, expression pattern, abiotic stress

Xian-yang LI, Hao LIU, Fei HE, Xue WANG, Ming-na LI, Rui-cai LONG, Jun-mei KANG, Qing-chuan YANG, Lin CHEN. Identification and expression pattern of the WRKY transcription factor family in Medicago sativa[J]. Acta Prataculturae Sinica, 2024, 33(4): 154-170.

Figures/Tables 10

References 52

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营养成分 Nutrient content	溶质 Solute	浓度 Concentration （μmol·L^-1）
大量元素 Major element	KNO₃	2500
	CaCl₂·2H₂O	250
	MgSO₄·7H₂O	1000
	KH₂PO₄	500
微量元素 Minor element	H₃BO₃	30
	MnSO₄·H₂O	5
	ZnSO₄·7H₂O	1
	CuSO₄·5H₂O	1
	Na₂MoO₄·2H₂O	0.7
铁盐 Ferric salt	FeSO₄·7H₂O	100
铁盐 Ferric salt	EDTA-Na₂	100

营养成分 Nutrient content	溶质 Solute	浓度 Concentration （μmol·L^-1）
大量元素 Major element	KNO₃	2500
	CaCl₂·2H₂O	250
	MgSO₄·7H₂O	1000
	KH₂PO₄	500
微量元素 Minor element	H₃BO₃	30
	MnSO₄·H₂O	5
	ZnSO₄·7H₂O	1
	CuSO₄·5H₂O	1
	Na₂MoO₄·2H₂O	0.7
铁盐 Ferric salt	FeSO₄·7H₂O	100
铁盐 Ferric salt	EDTA-Na₂	100

基因登录号 Gene ID	基因名 Gene name	PL （aa）	Mw （kDa）	pI	Sl	基因登录号 Gene ID	基因名 Gene name	PL （aa）	Mw （kDa）	pI	Sl
MS.gene54277	MsWRKY1	343	37.21	9.62	Nucl	MS.gene041042	MsWRKY100	270	29.83	9.48	Nucl
MS.gene49339	MsWRKY2	360	40.73	9.75	Nucl	MS.gene066380	MsWRKY101	419	45.87	7.25	Nucl
MS.gene005502	MsWRKY3	327	36.59	6.16	Nucl	MS.gene43637	MsWRKY102	360	40.10	5.65	Nucl
MS.gene066268	MsWRKY4	311	34.75	5.86	Nucl	MS.gene67236	MsWRKY103	547	60.63	6.61	Nucl
MS.gene016151	MsWRKY5	162	18.68	5.76	Nucl	MS.gene63867	MsWRKY104	548	59.61	8.61	Nucl
MS.gene54046	MsWRKY6	343	37.22	9.62	Nucl	MS.gene38063	MsWRKY105	386	42.86	6.25	Nucl
MS.gene50585	MsWRKY7	357	40.31	9.80	Nucl	MS.gene38065	MsWRKY106	278	32.04	9.23	Nucl
MS.gene07321	MsWRKY8	177	19.87	9.15	Nucl	MS.gene72198	MsWRKY107	502	55.25	7.68	Nucl
MS.gene60622	MsWRKY9	311	34.73	5.86	Nucl	MS.gene55314	MsWRKY108	434	47.53	6.71	Nucl
MS.gene051544	MsWRKY10	162	18.68	5.95	Nucl	MS.gene55386	MsWRKY109	273	30.35	6.31	Nucl
MS.gene86270	MsWRKY11	121	13.98	9.61	Cyto	MS.gene066778	MsWRKY110	273	30.35	6.31	Nucl
MS.gene86268	MsWRKY12	343	37.19	9.62	Nucl	MS.gene55387	MsWRKY111	164	18.34	7.11	Nucl
MS.gene036149	MsWRKY13	219	24.20	9.49	Mito	MS.gene61935	MsWRKY112	360	40.03	5.73	Nucl
MS.gene20870	MsWRKY14	303	33.72	6.75	Chlo	MS.gene99711	MsWRKY113	546	60.50	6.61	Nucl
MS.gene49162	MsWRKY15	311	34.73	5.86	Nucl	MS.gene81399	MsWRKY114	548	59.67	8.61	Nucl
MS.gene021549	MsWRKY16	343	37.19	9.62	Nucl	MS.gene81214	MsWRKY115	386	42.98	6.51	Nucl
MS.gene021548	MsWRKY17	343	37.19	9.62	Nucl	MS.gene015188	MsWRKY116	216	24.03	9.59	Nucl
MS.gene39903	MsWRKY18	360	40.72	9.80	Nucl	MS.gene85887	MsWRKY117	434	47.59	6.98	Nucl
MS.gene050353	MsWRKY19	177	19.87	9.15	Nucl	MS.gene99689	MsWRKY118	348	39.07	6.83	Nucl
MS.gene006220	MsWRKY20	311	34.65	5.86	Nucl	MS.gene010021	MsWRKY119	360	40.10	5.65	Nucl
MS.gene00145	MsWRKY21	324	35.74	8.54	Nucl	MS.gene009725	MsWRKY120	545	60.41	6.84	Nucl
MS.gene00061	MsWRKY22	386	42.82	5.95	Nucl	MS.gene057071	MsWRKY121	309	35.24	6.64	Nucl
MS.gene27204	MsWRKY23	557	60.02	7.70	Nucl	MS.gene48076	MsWRKY122	548	59.55	8.61	Nucl
MS.gene002619	MsWRKY24	220	24.28	5.08	Nucl	MS.gene38195	MsWRKY123	386	42.90	6.37	Nucl
MS.gene67724	MsWRKY25	324	35.70	8.53	Nucl	MS.gene38194	MsWRKY124	278	32.00	9.18	Nucl
MS.gene058334	MsWRKY26	465	51.44	5.94	Nucl	MS.gene53106	MsWRKY125	434	47.55	6.77	Nucl
MS.gene002992	MsWRKY27	563	60.76	7.70	Nucl	MS.gene53174	MsWRKY126	278	31.03	7.15	Nucl
MS.gene01605	MsWRKY28	220	24.28	5.08	Nucl	MS.gene023746	MsWRKY127	359	39.97	5.65	Nucl
MS.gene073173	MsWRKY29	261	28.88	5.85	Nucl	MS.gene060237	MsWRKY128	546	60.54	6.65	Nucl
MS.gene55979	MsWRKY30	322	35.36	8.18	Nucl	MS.gene020691	MsWRKY129	548	59.55	8.61	Nucl
MS.gene55899	MsWRKY31	477	52.71	5.98	Nucl	MS.gene020899	MsWRKY130	386	42.98	6.51	Nucl
MS.gene00786	MsWRKY32	563	60.76	7.70	Nucl	MS.gene020902	MsWRKY131	282	32.31	8.95	Nucl
MS.gene03111	MsWRKY33	220	24.28	5.08	Nucl	MS.gene050835	MsWRKY132	265	29.54	6.79	Nucl
MS.gene058246	MsWRKY34	378	41.69	6.35	Nucl	MS.gene09792	MsWRKY133	396	44.46	8.25	Nucl
MS.gene67885	MsWRKY35	307	34.22	6.92	Nucl	MS.gene007479	MsWRKY134	436	48.59	7.55	Nucl
MS.gene02354	MsWRKY36	563	60.74	7.70	Nucl	MS.gene024036	MsWRKY135	676	74.78	6.03	Nucl
MS.gene004297	MsWRKY37	220	24.28	5.08	Nucl	MS.gene024035	MsWRKY136	724	80.37	6.44	Nucl
MS.gene56264	MsWRKY38	701	76.01	6.13	Nucl	MS.gene87798	MsWRKY137	409	45.77	8.29	Nucl
MS.gene064637	MsWRKY39	595	64.76	6.58	Nucl	MS.gene99619	MsWRKY138	313	35.26	5.88	Nucl
MS.gene41592	MsWRKY40	511	57.09	6.29	Nucl	MS.gene021786	MsWRKY139	483	53.14	6.58	Nucl
MS.gene84141	MsWRKY41	340	38.22	6.07	Nucl	MS.gene74774	MsWRKY140	581	64.60	6.42	Nucl
MS.gene049409	MsWRKY42	324	36.89	5.28	Nucl	MS.gene024451	MsWRKY141	333	38.04	6.68	Nucl
MS.gene049350	MsWRKY43	243	27.80	8.31	Nucl	MS.gene28630	MsWRKY142	411	44.60	6.42	Nucl
MS.gene32785	MsWRKY44	164	18.76	5.37	Nucl	MS.gene09793	MsWRKY143	438	48.85	7.96	Nucl
MS.gene014903	MsWRKY45	293	32.92	8.70	Nucl	MS.gene050168	MsWRKY144	438	48.85	7.96	Nucl
MS.gene42609	MsWRKY46	293	32.92	8.70	Nucl	MS.gene007480	MsWRKY145	437	48.72	7.96	Nucl
MS.gene014722	MsWRKY47	452	48.67	9.87	Nucl	MS.gene033472	MsWRKY146	676	74.80	6.03	Nucl
MS.gene009477	MsWRKY48	702	76.15	6.08	Nucl	MS.gene033471	MsWRKY147	616	68.51	6.57	Nucl
MS.gene68485	MsWRKY49	519	56.93	6.42	Nucl	MS.gene85464	MsWRKY148	269	30.01	9.33	Nucl
MS.gene049457	MsWRKY50	512	57.19	6.25	Nucl	MS.gene42866	MsWRKY149	467	52.28	7.13	Nucl
MS.gene81987	MsWRKY51	342	38.45	6.21	Nucl	MS.gene46172	MsWRKY150	296	33.65	5.63	Nucl
MS.gene06539	MsWRKY52	324	36.89	5.28	Nucl	MS.gene057556	MsWRKY151	523	57.23	6.75	Nucl
MS.gene06469	MsWRKY53	243	27.80	8.31	Nucl	MS.gene043270	MsWRKY152	582	64.68	6.20	Nucl
MS.gene63519	MsWRKY54	702	76.23	6.06	Nucl	MS.gene050640	MsWRKY153	333	37.98	6.68	Nucl
MS.gene66170	MsWRKY55	595	64.77	6.58	Nucl	MS.gene072683	MsWRKY154	295	33.20	6.66	Nucl
MS.gene008580	MsWRKY56	506	56.68	6.25	Nucl	MS.gene20234	MsWRKY155	437	48.72	7.96	Nucl
MS.gene06753	MsWRKY57	340	38.30	6.21	Nucl	MS.gene51163	MsWRKY156	676	74.83	5.98	Nucl
MS.gene06784	MsWRKY58	324	36.92	5.28	Nucl	MS.gene51164	MsWRKY157	617	68.59	6.44	Nucl
MS.gene066523	MsWRKY59	98	11.38	9.32	Cyto	MS.gene51229	MsWRKY158	329	36.70	5.83	Nucl
MS.gene066525	MsWRKY60	98	11.38	9.32	Cyto	MS.gene42130	MsWRKY159	546	61.13	6.36	Nucl
MS.gene51632	MsWRKY61	704	76.41	6.10	Nucl	MS.gene96858	MsWRKY160	283	31.98	5.77	Nucl
MS.gene69700	MsWRKY62	595	64.76	6.48	Nucl	MS.gene001947	MsWRKY161	170	19.46	9.25	Nucl
MS.gene38536	MsWRKY63	511	57.15	6.25	Nucl	MS.gene45218	MsWRKY162	482	53.08	6.75	Nucl
MS.gene013439	MsWRKY64	324	36.89	5.28	Nucl	MS.gene79630	MsWRKY163	583	64.79	6.20	Nucl
MS.gene013373	MsWRKY65	243	27.80	8.31	Nucl	MS.gene22803	MsWRKY164	333	37.96	6.68	Nucl
MS.gene37404	MsWRKY66	164	18.83	5.24	Nucl	MS.gene072639	MsWRKY165	411	44.62	6.42	Nucl
MS.gene80192	MsWRKY67	293	32.90	8.70	Nucl	MS.gene28828	MsWRKY166	288	32.43	6.39	Nucl
MS.gene073017	MsWRKY68	452	48.66	9.87	Nucl	MS.gene22477	MsWRKY167	437	48.72	7.96	Nucl
MS.gene06061	MsWRKY69	361	41.20	5.27	Nucl	MS.gene52577	MsWRKY168	660	73.02	6.06	Nucl
MS.gene06012	MsWRKY70	274	31.17	6.71	Nucl	MS.gene52576	MsWRKY169	711	78.81	7.13	Nucl
MS.gene70298	MsWRKY71	235	26.72	8.92	Nucl	MS.gene87017	MsWRKY170	534	60.01	6.64	Nucl
MS.gene27827	MsWRKY72	244	27.39	8.22	Nucl	MS.gene055272	MsWRKY171	286	32.53	5.73	Nucl
MS.gene36464	MsWRKY73	349	39.33	6.39	Nucl	MS.gene97610	MsWRKY172	388	42.53	5.91	Nucl
MS.gene36419	MsWRKY74	483	54.10	5.43	Nucl	MS.gene021671	MsWRKY173	581	64.61	6.16	Nucl
MS.gene006821	MsWRKY75	380	41.96	8.57	Nucl	MS.gene012661	MsWRKY174	495	53.74	4.99	Nucl
MS.gene048916	MsWRKY76	361	41.20	5.27	Nucl	MS.gene035590	MsWRKY175	518	55.99	6.90	Nucl
MS.gene048869	MsWRKY77	274	31.23	6.46	Nucl	MS.gene56844	MsWRKY176	1028	111.57	9.27	Nucl
MS.gene06130	MsWRKY78	291	33.57	9.24	Nucl	MS.gene011781	MsWRKY177	337	36.70	9.74	Nucl
MS.gene58154	MsWRKY79	235	26.71	8.92	Nucl	MS.gene019695	MsWRKY178	223	24.59	5.45	Nucl
MS.gene028327	MsWRKY80	291	32.30	8.36	Nucl	MS.gene055421	MsWRKY179	227	25.78	8.15	Chlo
MS.gene028371	MsWRKY81	491	54.96	5.37	Nucl	MS.gene68948	MsWRKY180	494	53.67	4.99	Nucl
MS.gene83143	MsWRKY82	319	35.90	9.24	Nucl	MS.gene79844	MsWRKY181	223	24.59	5.45	Nucl
MS.gene82340	MsWRKY83	319	35.90	9.24	Nucl	MS.gene99163	MsWRKY182	227	25.78	8.15	Chlo
MS.gene56502	MsWRKY84	384	42.11	8.38	Nucl	MS.gene042165	MsWRKY183	495	53.74	4.99	Nucl
MS.gene55786	MsWRKY85	361	41.19	5.27	Nucl	MS.gene073340	MsWRKY184	314	34.62	9.85	Nucl
MS.gene55745	MsWRKY86	274	31.17	6.71	Nucl	MS.gene061258	MsWRKY185	426	46.97	5.86	Nucl
MS.gene030702	MsWRKY87	235	26.68	9.05	Nucl	MS.gene90475	MsWRKY186	337	36.70	9.74	Nucl
MS.gene058840	MsWRKY88	244	27.39	8.22	Nucl	MS.gene68851	MsWRKY187	495	53.74	4.99	Nucl
MS.gene023126	MsWRKY89	291	32.29	8.36	Nucl	MS.gene012560	MsWRKY188	518	55.99	6.90	Nucl
MS.gene28744	MsWRKY90	483	54.11	5.36	Nucl	MS.gene001561	MsWRKY189	518	55.99	6.90	Nucl
MS.gene33668	MsWRKY91	378	42.93	8.02	Nucl	MS.gene57898	MsWRKY190	518	55.99	6.90	Nucl
MS.gene069576	MsWRKY92	362	41.30	5.19	Nucl	MS.gene012558	MsWRKY191	518	55.99	6.90	Nucl
MS.gene89711	MsWRKY93	274	31.23	6.46	Nucl	MS.gene041947	MsWRKY192	337	36.70	9.74	Nucl
MS.gene28166	MsWRKY94	116	13.81	10.02	Cyto	MS.gene022631	MsWRKY193	227	25.78	8.15	Chlo
MS.gene023600	MsWRKY95	244	27.39	8.53	Nucl	MS.gene40378	MsWRKY194	324	34.40	9.91	Nucl
MS.gene35131	MsWRKY96	347	39.08	6.35	Nucl	MS.gene32696	MsWRKY195	293	32.89	8.65	Nucl
MS.gene35081	MsWRKY97	490	54.80	5.32	Nucl	MS.gene050416	MsWRKY196	295	33.21	6.66	Nucl
MS.gene068282	MsWRKY98	321	36.56	8.75	Nucl	MS.gene07075	MsWRKY197	295	33.21	6.66	Nucl
MS.gene059659	MsWRKY99	216	24.03	9.59	Nucl	MS.gene95234	MsWRKY198	188	22.22	9.56	Chlo

基因登录号 Gene ID	基因名 Gene name	PL （aa）	Mw （kDa）	pI	Sl	基因登录号 Gene ID	基因名 Gene name	PL （aa）	Mw （kDa）	pI	Sl
MS.gene54277	MsWRKY1	343	37.21	9.62	Nucl	MS.gene041042	MsWRKY100	270	29.83	9.48	Nucl
MS.gene49339	MsWRKY2	360	40.73	9.75	Nucl	MS.gene066380	MsWRKY101	419	45.87	7.25	Nucl
MS.gene005502	MsWRKY3	327	36.59	6.16	Nucl	MS.gene43637	MsWRKY102	360	40.10	5.65	Nucl
MS.gene066268	MsWRKY4	311	34.75	5.86	Nucl	MS.gene67236	MsWRKY103	547	60.63	6.61	Nucl
MS.gene016151	MsWRKY5	162	18.68	5.76	Nucl	MS.gene63867	MsWRKY104	548	59.61	8.61	Nucl
MS.gene54046	MsWRKY6	343	37.22	9.62	Nucl	MS.gene38063	MsWRKY105	386	42.86	6.25	Nucl
MS.gene50585	MsWRKY7	357	40.31	9.80	Nucl	MS.gene38065	MsWRKY106	278	32.04	9.23	Nucl
MS.gene07321	MsWRKY8	177	19.87	9.15	Nucl	MS.gene72198	MsWRKY107	502	55.25	7.68	Nucl
MS.gene60622	MsWRKY9	311	34.73	5.86	Nucl	MS.gene55314	MsWRKY108	434	47.53	6.71	Nucl
MS.gene051544	MsWRKY10	162	18.68	5.95	Nucl	MS.gene55386	MsWRKY109	273	30.35	6.31	Nucl
MS.gene86270	MsWRKY11	121	13.98	9.61	Cyto	MS.gene066778	MsWRKY110	273	30.35	6.31	Nucl
MS.gene86268	MsWRKY12	343	37.19	9.62	Nucl	MS.gene55387	MsWRKY111	164	18.34	7.11	Nucl
MS.gene036149	MsWRKY13	219	24.20	9.49	Mito	MS.gene61935	MsWRKY112	360	40.03	5.73	Nucl
MS.gene20870	MsWRKY14	303	33.72	6.75	Chlo	MS.gene99711	MsWRKY113	546	60.50	6.61	Nucl
MS.gene49162	MsWRKY15	311	34.73	5.86	Nucl	MS.gene81399	MsWRKY114	548	59.67	8.61	Nucl
MS.gene021549	MsWRKY16	343	37.19	9.62	Nucl	MS.gene81214	MsWRKY115	386	42.98	6.51	Nucl
MS.gene021548	MsWRKY17	343	37.19	9.62	Nucl	MS.gene015188	MsWRKY116	216	24.03	9.59	Nucl
MS.gene39903	MsWRKY18	360	40.72	9.80	Nucl	MS.gene85887	MsWRKY117	434	47.59	6.98	Nucl
MS.gene050353	MsWRKY19	177	19.87	9.15	Nucl	MS.gene99689	MsWRKY118	348	39.07	6.83	Nucl
MS.gene006220	MsWRKY20	311	34.65	5.86	Nucl	MS.gene010021	MsWRKY119	360	40.10	5.65	Nucl
MS.gene00145	MsWRKY21	324	35.74	8.54	Nucl	MS.gene009725	MsWRKY120	545	60.41	6.84	Nucl
MS.gene00061	MsWRKY22	386	42.82	5.95	Nucl	MS.gene057071	MsWRKY121	309	35.24	6.64	Nucl
MS.gene27204	MsWRKY23	557	60.02	7.70	Nucl	MS.gene48076	MsWRKY122	548	59.55	8.61	Nucl
MS.gene002619	MsWRKY24	220	24.28	5.08	Nucl	MS.gene38195	MsWRKY123	386	42.90	6.37	Nucl
MS.gene67724	MsWRKY25	324	35.70	8.53	Nucl	MS.gene38194	MsWRKY124	278	32.00	9.18	Nucl
MS.gene058334	MsWRKY26	465	51.44	5.94	Nucl	MS.gene53106	MsWRKY125	434	47.55	6.77	Nucl
MS.gene002992	MsWRKY27	563	60.76	7.70	Nucl	MS.gene53174	MsWRKY126	278	31.03	7.15	Nucl
MS.gene01605	MsWRKY28	220	24.28	5.08	Nucl	MS.gene023746	MsWRKY127	359	39.97	5.65	Nucl
MS.gene073173	MsWRKY29	261	28.88	5.85	Nucl	MS.gene060237	MsWRKY128	546	60.54	6.65	Nucl
MS.gene55979	MsWRKY30	322	35.36	8.18	Nucl	MS.gene020691	MsWRKY129	548	59.55	8.61	Nucl
MS.gene55899	MsWRKY31	477	52.71	5.98	Nucl	MS.gene020899	MsWRKY130	386	42.98	6.51	Nucl
MS.gene00786	MsWRKY32	563	60.76	7.70	Nucl	MS.gene020902	MsWRKY131	282	32.31	8.95	Nucl
MS.gene03111	MsWRKY33	220	24.28	5.08	Nucl	MS.gene050835	MsWRKY132	265	29.54	6.79	Nucl
MS.gene058246	MsWRKY34	378	41.69	6.35	Nucl	MS.gene09792	MsWRKY133	396	44.46	8.25	Nucl
MS.gene67885	MsWRKY35	307	34.22	6.92	Nucl	MS.gene007479	MsWRKY134	436	48.59	7.55	Nucl
MS.gene02354	MsWRKY36	563	60.74	7.70	Nucl	MS.gene024036	MsWRKY135	676	74.78	6.03	Nucl
MS.gene004297	MsWRKY37	220	24.28	5.08	Nucl	MS.gene024035	MsWRKY136	724	80.37	6.44	Nucl
MS.gene56264	MsWRKY38	701	76.01	6.13	Nucl	MS.gene87798	MsWRKY137	409	45.77	8.29	Nucl
MS.gene064637	MsWRKY39	595	64.76	6.58	Nucl	MS.gene99619	MsWRKY138	313	35.26	5.88	Nucl
MS.gene41592	MsWRKY40	511	57.09	6.29	Nucl	MS.gene021786	MsWRKY139	483	53.14	6.58	Nucl
MS.gene84141	MsWRKY41	340	38.22	6.07	Nucl	MS.gene74774	MsWRKY140	581	64.60	6.42	Nucl
MS.gene049409	MsWRKY42	324	36.89	5.28	Nucl	MS.gene024451	MsWRKY141	333	38.04	6.68	Nucl
MS.gene049350	MsWRKY43	243	27.80	8.31	Nucl	MS.gene28630	MsWRKY142	411	44.60	6.42	Nucl
MS.gene32785	MsWRKY44	164	18.76	5.37	Nucl	MS.gene09793	MsWRKY143	438	48.85	7.96	Nucl
MS.gene014903	MsWRKY45	293	32.92	8.70	Nucl	MS.gene050168	MsWRKY144	438	48.85	7.96	Nucl
MS.gene42609	MsWRKY46	293	32.92	8.70	Nucl	MS.gene007480	MsWRKY145	437	48.72	7.96	Nucl
MS.gene014722	MsWRKY47	452	48.67	9.87	Nucl	MS.gene033472	MsWRKY146	676	74.80	6.03	Nucl
MS.gene009477	MsWRKY48	702	76.15	6.08	Nucl	MS.gene033471	MsWRKY147	616	68.51	6.57	Nucl
MS.gene68485	MsWRKY49	519	56.93	6.42	Nucl	MS.gene85464	MsWRKY148	269	30.01	9.33	Nucl
MS.gene049457	MsWRKY50	512	57.19	6.25	Nucl	MS.gene42866	MsWRKY149	467	52.28	7.13	Nucl
MS.gene81987	MsWRKY51	342	38.45	6.21	Nucl	MS.gene46172	MsWRKY150	296	33.65	5.63	Nucl
MS.gene06539	MsWRKY52	324	36.89	5.28	Nucl	MS.gene057556	MsWRKY151	523	57.23	6.75	Nucl
MS.gene06469	MsWRKY53	243	27.80	8.31	Nucl	MS.gene043270	MsWRKY152	582	64.68	6.20	Nucl
MS.gene63519	MsWRKY54	702	76.23	6.06	Nucl	MS.gene050640	MsWRKY153	333	37.98	6.68	Nucl
MS.gene66170	MsWRKY55	595	64.77	6.58	Nucl	MS.gene072683	MsWRKY154	295	33.20	6.66	Nucl
MS.gene008580	MsWRKY56	506	56.68	6.25	Nucl	MS.gene20234	MsWRKY155	437	48.72	7.96	Nucl
MS.gene06753	MsWRKY57	340	38.30	6.21	Nucl	MS.gene51163	MsWRKY156	676	74.83	5.98	Nucl
MS.gene06784	MsWRKY58	324	36.92	5.28	Nucl	MS.gene51164	MsWRKY157	617	68.59	6.44	Nucl
MS.gene066523	MsWRKY59	98	11.38	9.32	Cyto	MS.gene51229	MsWRKY158	329	36.70	5.83	Nucl
MS.gene066525	MsWRKY60	98	11.38	9.32	Cyto	MS.gene42130	MsWRKY159	546	61.13	6.36	Nucl
MS.gene51632	MsWRKY61	704	76.41	6.10	Nucl	MS.gene96858	MsWRKY160	283	31.98	5.77	Nucl
MS.gene69700	MsWRKY62	595	64.76	6.48	Nucl	MS.gene001947	MsWRKY161	170	19.46	9.25	Nucl
MS.gene38536	MsWRKY63	511	57.15	6.25	Nucl	MS.gene45218	MsWRKY162	482	53.08	6.75	Nucl
MS.gene013439	MsWRKY64	324	36.89	5.28	Nucl	MS.gene79630	MsWRKY163	583	64.79	6.20	Nucl
MS.gene013373	MsWRKY65	243	27.80	8.31	Nucl	MS.gene22803	MsWRKY164	333	37.96	6.68	Nucl
MS.gene37404	MsWRKY66	164	18.83	5.24	Nucl	MS.gene072639	MsWRKY165	411	44.62	6.42	Nucl
MS.gene80192	MsWRKY67	293	32.90	8.70	Nucl	MS.gene28828	MsWRKY166	288	32.43	6.39	Nucl
MS.gene073017	MsWRKY68	452	48.66	9.87	Nucl	MS.gene22477	MsWRKY167	437	48.72	7.96	Nucl
MS.gene06061	MsWRKY69	361	41.20	5.27	Nucl	MS.gene52577	MsWRKY168	660	73.02	6.06	Nucl
MS.gene06012	MsWRKY70	274	31.17	6.71	Nucl	MS.gene52576	MsWRKY169	711	78.81	7.13	Nucl
MS.gene70298	MsWRKY71	235	26.72	8.92	Nucl	MS.gene87017	MsWRKY170	534	60.01	6.64	Nucl
MS.gene27827	MsWRKY72	244	27.39	8.22	Nucl	MS.gene055272	MsWRKY171	286	32.53	5.73	Nucl
MS.gene36464	MsWRKY73	349	39.33	6.39	Nucl	MS.gene97610	MsWRKY172	388	42.53	5.91	Nucl
MS.gene36419	MsWRKY74	483	54.10	5.43	Nucl	MS.gene021671	MsWRKY173	581	64.61	6.16	Nucl
MS.gene006821	MsWRKY75	380	41.96	8.57	Nucl	MS.gene012661	MsWRKY174	495	53.74	4.99	Nucl
MS.gene048916	MsWRKY76	361	41.20	5.27	Nucl	MS.gene035590	MsWRKY175	518	55.99	6.90	Nucl
MS.gene048869	MsWRKY77	274	31.23	6.46	Nucl	MS.gene56844	MsWRKY176	1028	111.57	9.27	Nucl
MS.gene06130	MsWRKY78	291	33.57	9.24	Nucl	MS.gene011781	MsWRKY177	337	36.70	9.74	Nucl
MS.gene58154	MsWRKY79	235	26.71	8.92	Nucl	MS.gene019695	MsWRKY178	223	24.59	5.45	Nucl
MS.gene028327	MsWRKY80	291	32.30	8.36	Nucl	MS.gene055421	MsWRKY179	227	25.78	8.15	Chlo
MS.gene028371	MsWRKY81	491	54.96	5.37	Nucl	MS.gene68948	MsWRKY180	494	53.67	4.99	Nucl
MS.gene83143	MsWRKY82	319	35.90	9.24	Nucl	MS.gene79844	MsWRKY181	223	24.59	5.45	Nucl
MS.gene82340	MsWRKY83	319	35.90	9.24	Nucl	MS.gene99163	MsWRKY182	227	25.78	8.15	Chlo
MS.gene56502	MsWRKY84	384	42.11	8.38	Nucl	MS.gene042165	MsWRKY183	495	53.74	4.99	Nucl
MS.gene55786	MsWRKY85	361	41.19	5.27	Nucl	MS.gene073340	MsWRKY184	314	34.62	9.85	Nucl
MS.gene55745	MsWRKY86	274	31.17	6.71	Nucl	MS.gene061258	MsWRKY185	426	46.97	5.86	Nucl
MS.gene030702	MsWRKY87	235	26.68	9.05	Nucl	MS.gene90475	MsWRKY186	337	36.70	9.74	Nucl
MS.gene058840	MsWRKY88	244	27.39	8.22	Nucl	MS.gene68851	MsWRKY187	495	53.74	4.99	Nucl
MS.gene023126	MsWRKY89	291	32.29	8.36	Nucl	MS.gene012560	MsWRKY188	518	55.99	6.90	Nucl
MS.gene28744	MsWRKY90	483	54.11	5.36	Nucl	MS.gene001561	MsWRKY189	518	55.99	6.90	Nucl
MS.gene33668	MsWRKY91	378	42.93	8.02	Nucl	MS.gene57898	MsWRKY190	518	55.99	6.90	Nucl
MS.gene069576	MsWRKY92	362	41.30	5.19	Nucl	MS.gene012558	MsWRKY191	518	55.99	6.90	Nucl
MS.gene89711	MsWRKY93	274	31.23	6.46	Nucl	MS.gene041947	MsWRKY192	337	36.70	9.74	Nucl
MS.gene28166	MsWRKY94	116	13.81	10.02	Cyto	MS.gene022631	MsWRKY193	227	25.78	8.15	Chlo
MS.gene023600	MsWRKY95	244	27.39	8.53	Nucl	MS.gene40378	MsWRKY194	324	34.40	9.91	Nucl
MS.gene35131	MsWRKY96	347	39.08	6.35	Nucl	MS.gene32696	MsWRKY195	293	32.89	8.65	Nucl
MS.gene35081	MsWRKY97	490	54.80	5.32	Nucl	MS.gene050416	MsWRKY196	295	33.21	6.66	Nucl
MS.gene068282	MsWRKY98	321	36.56	8.75	Nucl	MS.gene07075	MsWRKY197	295	33.21	6.66	Nucl
MS.gene059659	MsWRKY99	216	24.03	9.59	Nucl	MS.gene95234	MsWRKY198	188	22.22	9.56	Chlo

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[2]	Yan LI, Fu-long MA, Lu HAN, Hai-zhen WANG. Productivity and adaptability of ‘WL’ alfalfa varieties with different fall dormancy in the extremely arid region of Southern Xinjiang [J]. Acta Prataculturae Sinica, 2024, 33(3): 139-149.
[3]	Xue WANG, Xiao-jing LIU, Jing WANG, Yong WU, Chang-chun TONG. Root and carbon-nitrogen metabolism characteristics of alfalfa-oat mixed stands under continuous intercropping [J]. Acta Prataculturae Sinica, 2024, 33(3): 85-96.
[4]	Ying TANG, Xiao-jing LIU, Ya-jiao ZHAO, Lin DONG. Characteristics and driving factors of lactic acid bacteria communities in silage made from alfalfa in different regions of Gansu Province [J]. Acta Prataculturae Sinica, 2024, 33(2): 112-124.
[5]	Kong-qin WEI, Jun-wei ZHAO, Qian-bing ZHANG. Effects of phosphorus application on soil respiration rate and active organic carbon components of alfalfa [J]. Acta Prataculturae Sinica, 2024, 33(2): 80-92.
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