Acta Prataculturae Sinica ›› 2024, Vol. 33 ›› Issue (10): 135-146.DOI: 10.11686/cyxb2023426
Zheng WANG(), Xin LI, Jian-gui ZHANG, Ji-kuan CHAI, Gui-qin ZHAO, Kui-ju NIU(
)
Received:
2023-11-14
Revised:
2024-01-18
Online:
2024-10-20
Published:
2024-07-15
Contact:
Kui-ju NIU
Zheng WANG, Xin LI, Jian-gui ZHANG, Ji-kuan CHAI, Gui-qin ZHAO, Kui-ju NIU. Exogenous melatonin mediates the antioxidant system and phenylpropanoid metabolism to induce resistance to leaf spot disease in oat[J]. Acta Prataculturae Sinica, 2024, 33(10): 135-146.
引物Primer | 上游引物Upstream primer | 下游引物Downstream primer |
---|---|---|
超氧化物歧化酶SOD | TGTCGATCGGGCAGATGATG | CCCCACCACCAGGTTTCATT |
过氧化物酶POD | AACGTGCAGAACCCGATCAT | AGCGGTAGTCCCTCTGATGT |
过氧化氢酶CAT | CGGAGTCCCTCCACATGTTC | TTGACGTAGTGGGACTTGCC |
抗坏血酸过氧化物酶APX | TTGTCGCTCTATCTGGTGGC | AGCCCCTCAGATTCTCCCTT |
苯丙氨酸解氨酶PAL | CTATCGATCTGCGCCACCTT | TGTGATTGGTGCTCAGGGTC |
4-香豆酸∶辅酶A连接酶4CL | AGGATGCTGCTGTCGTATCG | TGCGACGAACTGCTTGATCT |
肉桂酸-4-羟化酶C4H | AACTACGGCGACTTCATCCC | TCCTTGAAGAGCTTGAGGCG |
肌动蛋白基因The actin gene | GAGCGGGAAATTGTAAGGGAC | ATGGATGGCTGGAAGAGGAC |
Table 1 The qRT-PCR primer design
引物Primer | 上游引物Upstream primer | 下游引物Downstream primer |
---|---|---|
超氧化物歧化酶SOD | TGTCGATCGGGCAGATGATG | CCCCACCACCAGGTTTCATT |
过氧化物酶POD | AACGTGCAGAACCCGATCAT | AGCGGTAGTCCCTCTGATGT |
过氧化氢酶CAT | CGGAGTCCCTCCACATGTTC | TTGACGTAGTGGGACTTGCC |
抗坏血酸过氧化物酶APX | TTGTCGCTCTATCTGGTGGC | AGCCCCTCAGATTCTCCCTT |
苯丙氨酸解氨酶PAL | CTATCGATCTGCGCCACCTT | TGTGATTGGTGCTCAGGGTC |
4-香豆酸∶辅酶A连接酶4CL | AGGATGCTGCTGTCGTATCG | TGCGACGAACTGCTTGATCT |
肉桂酸-4-羟化酶C4H | AACTACGGCGACTTCATCCC | TCCTTGAAGAGCTTGAGGCG |
肌动蛋白基因The actin gene | GAGCGGGAAATTGTAAGGGAC | ATGGATGGCTGGAAGAGGAC |
Fig.4 The effect of exogenous melatonin on the disease index and chlorophyll content of oat leaves after inoculation with pathogenic bacteria during the seedling stage
Fig.5 Effects of exogenous melatonin on H2O2 content, superoxide anion production rate, and malondialdehyde content in leaves of oat seedlings inoculated with pathogenic bacteria
Fig.7 Effect of exogenous melatonin on the activity of phenylpropanoid metabolism related enzymes in oat leaves after inoculation with pathogenic bacteria during seedling stage
Fig.8 The effect of exogenous melatonin on the lignin, total phenol, and flavonoid content of oat leaves after inoculation with pathogenic bacteria during the seedling stage
Fig.9 The effect of exogenous melatonin on the relative expression of enzyme genes in oat leaves after inoculation with pathogenic bacteria during the seedling stage
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